H P Rohr

University of Innsbruck, Innsbruck, Tyrol, Austria

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Publications (159)199.39 Total impact

  • Article: Case 4
    H P Rohr · G Bartsch
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    ABSTRACT: Prostatic biopsies of two groups of 4 and 6 patients with benign prostatic hyperplasia (BPH) (average age 69.5 years) before hormonal treatment with cyproterone acetate (group A) or bromocryptine (2-bromo-α-ergocryptine) (group B), respectively (Figs. 1 and 2), were evaluated by stereologic methods. To assess the pituitary gonadal function of the patients, the following steroid determinations were performed 1 day before the biopsy: luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin, 17β-estradiol, testosterone. The drugs were administered over a period of 8 weeks, then suprapubic prostatectomy was performed.
    No preview · Article · Jul 2009 · Ultrastructural Pathology
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    ABSTRACT: ZusammenfassungStereologie eine neue morphologische Methode zum Studium der Funktion des NebenhodensStereologische Methoden erlauben eine Bestimmung von relativen und absoluten Werten von Volumina, Oberflchen und Zahl von Gewebs- und Zellkomponenten innerhalb des Nebenhodens. Die stereologischen Methoden, die grundstzlich auf den Methoden geometrischer Wahrscheinlichkeit beruhen, werden dargestellt. Ein stereo-logisches Modell wurde für den Nebenhodenkopf der Ratte erarbeitet. Dieses gibt Aus-kunft über relative Verhltnisse von Volumina, Oberflchen und numerischen Dichten der verschiedenen Gewebs- und Zellkomponenten des Nebenhodenkopfes der Ratte. Als experimentelles Anwendungsbeispiel wird der Einfluß einer Langzeithypophysek-tomie auf den Nebenhodenkopf der Ratte aufgezeigt. Die Moglichkeit einer Korrela-tion dieser quantitativen morphologischen Methoden mit biochemischen Methoden wird erörtert.
    No preview · Article · Apr 2009 · Andrologia
  • G Bartsch · I Wagner · H.P. ROHR
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    ABSTRACT: The alterations induced in principal cells of the epididymal head tissue of the rat by administration of a synthetic progestine (R2323, ethinyl-norgestrienone) were studied by electron microscopy. A total of 3 male, sex mature rats was applicated ethinyl-norgestrienone during a 3 months period in low dosage (60 μg) per day and was studied with 5 control animals. Stereological methods devised by Weibel (1969) and Rohr et al. (1976) were applied to light and electron micrographs. The calculated values are related to 1 cm3 of epididymal tissue, 1 cm3 of epididymal cell and 1 cm3 of epididymal cell cytoplasm. The Golgi apparatus was further subdivided; the values for the Golgi compartments are therefore refered to 1 cm3 of Golgi apparatus. In the treated animals there was, related to the unit of epididymal tissue, a significant decrease in the volume density of the interductular tissue, whereas that for the lumina was significantly higher. The volume density of the glandular epithelium remains unchanged. Refered to the unit volume of principal cell cytoplasm the rough endoplasmic reticulum shows a significant decrease by 6.5%, whereas the smooth endoplasmic reticulum is increased by 6.3%. The volume density of the lysosomes was calculated to be 4.3% (Control 2.5%). The stereological data of the Golgi apparatus indicate a vacuolar transformation: Increased volume of vacuoles, decreased volume fraction of saccules and vesicles. However, the volume density of the whole Golgi apparatus remains unchanged. An impaired secretory activity of the principal cell could be assumed. Während korrelierende biochemische Befunde über den Nebenhoden quantitativer Natur sind, beschränkt sich die bisherige morphologische Literatur in elektronenmikos-kopischer Hinsicht auf subjektiv erhobene deskriptive Befunde. Stereologische Metho-den erlauben eine Quantifizierung morphologischer Befunde (Weibel - 1969; Rohr et al. - 1976). In einer licht- und ultrastrukturellen morphometrischen Analyse wird über die Veränderungen des Nebenhodenkopfes unter Einfluß des synthetischen Gestagens Äthinyl-Norgestrienon (R2323, Fa. Roussel, France) berichtet. Die Ergebnisse von 3 männlichen, geschlechtsreifen behandelten Wisterratten (tägliche Zufuhr von 60 μg Äthinyl-Norgestrienon durch 3 Monate) wird mit jenen von 5 Kontrolltieren verglichen. Die errechneten Werte beziehen sich jeweils auf 1 cm3 Nebenhodengewebe, 1 cm3 Nebenhodenzelle und 1 cm3 Cytoplasma. Bei den mit dem synthetischen Gestagen Äthinyl-Norgestrienon behandelten Tieren zeigt sich, bezogen auf das Einheitsvolumen Nebenhodengewebe, eine signifikante Abnahme des interductulären Gewebes und eine signi-fikante Zunahme der Lumina der Nebenhodenkanälchen. Das Drüsenparenchym bleibt unverändert. Bezogen auf das Einheitsvolumen Cytoplasma findet sich eine signifikante Abnahme des rauhen endoplasmatischen Retikulums (um 6, 5%) und eine signifikante Zunahme des glatten endoplasmatischen Retikulums (um 6, 3%). Die Volumendichte der Lysosomen beträgt 4, 3% (Kontrolltier: 2, 5%). Die stereologischen Daten des Golgi-Apparates lassen eine vacuoläre Transformation dieser Zellorganelle annehmen; erhöhte Volumendichte für die Golgi Vacuolen, erniedrigte Volumendichte für die Golgi Säck-chen und Bläschen. Die Volumendichte des gesamten Golgiapparates bezogen auf das Einheitsvolumen Cytoplasma bleibt unverändert. Diese stereologischen Daten lassen eine verminderte sekretorische Aktivität der Hauptzellen – principal cells – des Nebenhodenkopfes unter dieser Versuchsanordnung annehmen.
    No preview · Article · Jan 2009 · Andrologia
  • A Schmassmann · G Mikuz · G Bartsch · C Gysin · H.P. ROHR
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    ABSTRACT: Desoxyribonukleinsäure-Gehalt morphologisch unterschiedlicher Spermatozoentypen bei normalen und subfertilen Männern 20 männliche Patienten (G2), bei denen man eine idiopathische Oligozoospermie festgestellt hatte, und 10 normale, fertile Männer (G***1) wurden untersucht. Der DNA-Gehalt von 50 nach Feulgen gefärbten Spermienköpfen pro Person wurde mit Hilfe eines Einzelzellphotometers gemessen. In einem zweiten Arbeitsgang wurde die Fläche, der Umfang, die Länge und die Breite der gleichen Spermienköpfe mit Hilfe eines halbautomatischen Bildanalyse-gerätes gemessen. Bei einem Vergleich zwischen G1 und G2 unter Berücksichtigung von nur morphologisch normalen Köpfen ist der mittlere DNA-Gehalt nur leicht reduziert in G2. Ganz im Gegen-teil dazu ist die DNA-Variation stark erhöht in G2. Unglücklicherweise, zeigen auch morphologisch defekte Köpfe eine erhöhte DNA-Variation. Bei einer Bestimmung der DNA-Variation in einem Samen mit vielen morphologisch defekten Köpfen ist deshalb eine gemeinsame Bestimmung von DNA-Gehalt und Kopfmorphologie notwendig. Dies kann nur mit Hilfe der Einzelzellzytophotometrie erreicht werden. Auf dieses Problem bezogen ist deshalb die Einzelzellzytophotometrie der Durchflußzytophotometrie überlegen. Viele Sper-mienköpfe mit stark abnormalem DNA-Gehalt zeigen keine abnormen Morphologiemerk-male. Diese subzellulären Veränderungen können nur mit einer DNA-Bestimmung aufge-zeigt werden.
    No preview · Article · Jan 2009 · Andrologia

  • No preview · Article · Feb 1994 · Progress in clinical and biological research
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    ABSTRACT: Morphometric studies show that benign human prostatic hyperplasia is a stromal disease caused by the activation of smooth muscle cells. This activation manifests itself in increased amounts of cytoplasmic organelles, which are preferably localized in the perinuclear region. Moreover, marked vesicular activity is present. Besides considerable overproduction of type I and III collagens, the architecture of the extracellular matrix is altered distinctly. In spite of strong evidence that androgens and estrogens regulate the growth of epithelial and stromal cells in the prostate and the induction of fibromuscular overgrowth in various animal models, the exact role of steroids in the pathogenesis of benign prostatic hyperplasia still remains to be elucidated.
    No preview · Article · Dec 1989 · Der Urologe
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    ABSTRACT: Unilateral testicular disease has been reported to damage the contralateral testis. In order to find out whether this detrimental influence is permanent in nature or can be avoided by therapeutic measures, and furthermore to quantify the damage, the following experiments were performed. Seventy-five rats were classified into the following five groups: I) testicular torsion persisting for eight hours; II) ipsilateral semicastration after torsion persisting for eight hours; III) semicastration; IV) sham operation as control; V) immunosuppression with azathioprine after torsion persisting for eight hours. The contralateral testes were removed two months later and perfused with fixative via the testicular artery. Stereologic techniques were employed to obtain quantitative morphologic data. Serum hormone levels were determined. The volume density of the contralateral germinal epithelium was not decreased two months after torsion for eight hours, torsion following by semicastration or torsion followed by immunosuppression. The same was true of the total volume of germinal epithelium per rat testis. The hormone levels remained essentially unchanged.
    No preview · Article · Nov 1987 · The Journal of Urology
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    ABSTRACT: In previous light and electron-microscopic analyses human benign prostatic hyperplasia was shown to be predominantly a stromal disease; the aim of the present study was to correlate the stereological data with the levels of the endogenous tissue hormones (androgens, estrogens, progesterone) in normal (N) and hyperplastic human prostatic tissues (BPH). BPH tissue specimens were obtained by open prostatectomy (n = 25); normal prostatic tissue was obtained from kidney donors (n = 5). No statistically significant difference was found between normal and hyperplastic tissue. Testosterone BPH 0.69 +/- 0.44, N 0.25 +/- 0.12; 5 alpha-dihydrotestosterone BPH 7.0 +/- 2.9, N 4.2 +/- 0.7; progesterone BPH 0.059 +/- 0.022, N 0.058 +/- 0.005; estrone BPH 0.10 +/- 0.03, N 0.14 +/- 0.03; estradiol BPH 0.07 +/- 0.02, N 0.05 +/- 0.02; estriol BPH 0.02 +/- 0.01, N 0.04 +/- 0.02. Using a Spearman rank correlation coefficient a statistical analysis was performed for age, weight of the prostate, absolute stereological data and the endogenous prostatic hormones. As can be seen from the statistical analysis there is a poor correlation for 5 alpha-dihydrotestosterone and the amount of the glandular epithelium; otherwise no correlation of the endogenous tissue hormones with the stereological data investigated was found. These data show that the stromal overgrowth of benign hyperplasia is not reflected in the tissue hormone levels.
    No preview · Article · Apr 1987 · The Journal of Urology
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    ABSTRACT: A combined light-microscopic and stereologic analysis of the canine prostate was performed under the following experimental conditions: intact and castrated dogs, spontaneous benign prostatic hyperplasia, intact and castrated dogs after treatment with testosterone, 5 alpha-dihydrotestosterone or 3 alpha-androstanediol in combination with estradiol. Regarding the absolute amounts of the glandular and stromal parts of the prostate as well as the glandular cells, no difference was found among the testosterone, 3 alpha-androstanediol and 5 alpha-dihydrotestosterone treated castrated dogs. Treatment with 5 alpha-dihydrotestosterone or 3 alpha-androstanediol in combination with 17 beta-estradiol induced a four-fold increase in glandular and a two-fold increase in stromal tissue. The glandular to stromal tissue ratio equals that found in spontaneous canine hyperplasia, which is indicative of the glandular type of spontaneous canine hyperplasia. Therefore, it can be stated that treatment with 5 alpha-dihydrotestosterone or 3 alpha-androstanediol combined with 17 beta-estradiol not only induces prostatic overgrowth but also leads to prostatic hyperplasia of the glandular type. However, the stereologic analysis of canine prostates following steroid administration shows that canine hyperplasia is primarily a glandular disease, while human benign prostatic hyperplasia shows more stromal activation.
    No preview · Article · Apr 1987 · The Journal of Urology
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    ABSTRACT: Objective and reproducible determinations of sperm motility and morphology are achieved using a semi-automatic image analysis system under optomanual control. The method was used to evaluate the short-and long-term effects of the operative treatment of varicocele on sperm density, motility and velocity in 27 men with left-sided varicosities. Whereas a significant improvement (p=0.0001) in sperm density was found within 3 months after surgery, almost no increases in sperm motility and velocity occurred. The actual post-operative improvement in sperm density did not correlate with the preoperative sperm counts (r=0.291). Between the preoperative values of motility and velocity and the postoperative improvement, however, a negative correlation of –0.431 and –0.759, respectively, was found. Considering the 21 patients, who were followed up for a minimum of 9 months, a sustained increase in sperm density, motility and velocity of at least 50% was observed in 14 (66.7%), 7 (33.3%) and 5 (23.8%), respectively. In general, the better results were obtained in the group with the poorer preoperative semen characteristics. However, only 4 patients (19%) showed a lasting improvement in all 3 parameters. Even more interesting was the sustained decrease in sperm velocity observed in 9 men (42.9%), who had rather good preoperative values. It is concluded, that a timely operative treatment of varicocele might prevent further impairment of semen quality. However, more research on the natural course of varicocele is necessary.
    No preview · Article · Aug 1986 · World Journal of Urology
  • G. Bartsch · R. Heilbronner · M. Hurt · H. P. Rohr
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    ABSTRACT: In previous light and electron-microscopic analyses, human benign prostatic hyperplasia was shown to be predominantly a stromal disease. Smooth muscle cell activation and stromal overgrowth was achieved in beagle dogs following estrogen administration; this experiment indicates that estrogens play a role in the induction of stromal growth in the prostate. In the present investigation, histological and electron-microscopic studies on the estrogen-induced enlargement of the prostate in a/J mice were performed. After treatment with estrogens (0.1 mg estradiol valerianate per week for 12 weeks) two types of stromal reaction patterns were observed: 1. tightly packed smooth muscle cells with formation of whorl-like nodules, 2. loose interstitial tissue surrounding the stromal and glandular cells. Electron microscopy of the stromal tissue reveals fibrocytes, smooth muscle cells and bundles of collagen fibres in between. The tightly packed smooth muscle cells are highly activated (increased amount of cytoplasmic organelles). Similar to the stromal tissue, the glandular cells do not show a homogeneous reaction to estrogen stimulation. If the glandular cells were surrounded by loose interstitial tissue, only mild metaplastic changes were observed; however, inside the whorl-like nodules of the smooth muscle cells, severe metaplastic changes were observed.
    No preview · Article · Aug 1986 · World Journal of Urology
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    ABSTRACT: A combined electron microscopic stereological and biochemical study of the smooth muscle cells of guinea pig seminal vesicles was performed in intact, castrated, castrated and dihydrotestosterone- or estradiol-treated adult animals. Castration led to cell atrophy as determined stereologically by a decreased single cell volume and biochemically by no change in DNA content coupled with an increase in the DNA concentration. Treatment of castrates with dihydrotestosterone restored both the stereological and biochemical parameters of the cell size to slightly supranormal levels. The estrogen-induced increase in muscle weight and DNA content appeared to be due only to hyperplasia of muscle cells and not to a proliferation of fibroblasts or to infiltration by inflammatory cells. In all treatment groups, including the estrogen-treated castrates, more than 95% of the cells in the tissue were smooth muscle cells, and there was no evidence that polyploidy contributed to changes in DNA levels. In addition, in the estrogen-treated muscles, DNA concentration remained high, and the stereologically determined cell size remained low. Therefore, both morphological and biochemical evidence indicate that androgen induces hypertrophy, whereas estrogen induces hyperplasia of muscle cells. The correction of stereological and biochemical data validates the application of stereological cell size determination for smooth muscle cells in organs that hardly can be separated into stromal and epithelial components; eg, the prostate.
    No preview · Article · Jan 1986 · The Prostate
  • H U Schweikert · P Totzauer · H P Rohr · G Bartsch
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    ABSTRACT: The growth and function of the human prostate is dependent upon a continuous supply of androgens, mainly 5 alpha-dihydrotestosterone, the 5 alpha-reduced metabolite of testosterone. Within the human prostate dihydrotestosterone is thought to be the intracellular mediator of androgen action. Although it is well documented that dihydrotestosterone is evenly distributed between the stromal and epithelial compartment of the prostate, the anatomical localization of dihydrotestosterone formation within the normal and hyperplastic prostate is still not established. To provide further insight into this problem we have measured, under conditions approximating the in vivo state, dihydrotestosterone formation in prostates obtained from 4 men with normal prostates and 36 men with benign prostatic hyperplasia. In addition to this we have performed histometric analysis of the cellular composition of the samples incubated, in order to correlate the morphological and the histochemical findings. Dihydrotestosterone is the major metabolite, and androstanediol and androstenedione were formed in smaller quantities. Under the given conditions metabolite formation from testosterone increased linearly for 60 minutes and the half maximum rate of dihydrotestosterone formation (Km) was observed at about 1.25 X 10(-6) M testosterone, a value similar to that reported for rat prostatic nuclei and human prostatic tissue. Dihydrotestosterone formation was higher in hyperplastic prostates than in the normal prostate. (Student's t test: p less than 0.05). The stroma in both the normal and hyperplastic tissue converts testosterone to dihydrotestosterone very actively. No significant relation was found between dihydrotestosterone formation and the per cent distribution of the stromal and epithelial compartment in any sample studied. In conclusion, our results are compatible first with the thesis that the rate of dihydrotestosterone formation is increased in the hyperplastic prostate and secondly with the concept that the rate of dihydrotestosterone formation is approximately the same in the epithelial and stromal compartments of the prostate.
    No preview · Article · Sep 1985 · The Journal of Urology
  • M Oberholzer · H Durrer · H P Rohr · P U Heitz

    No preview · Article · Feb 1985 · Acta histochemica. Supplementband
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    ABSTRACT: The problems and the interpretation of combined stereological-biochemical investigations in benign prostatic hyperplasia (BPH) research are discussed. As BPH is characterized by a primary stromal enlargement the role of the smooth muscle cell (SMC) is focussed. For determination of the single cell volume of the SMC several methods are applicable leading to different results. Thus interpretation of these results has to be done with caution. Finally as an example there is a discussion of a correlation of stereological-biochemical dates of human prostatic tissue samples.
    No preview · Article · Dec 1984 · Pathology - Research and Practice
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    ABSTRACT: A morphometric procedure is presented, which allows quantitative information to be obtained from the epidermis at the light microscope level. The application of this procedure to human skin grafted to the nude mouse revealed acanthosis of the grafted epidermis compared to the original donor skin. All epidermal layers were thicker, but the increase in the granular layer was especially marked. The ratio of the basement membrane surface to the epithelial surface showed no significant change. A possible explanation for the acanthosis of the graft might be the higher mechanical stress on the nude mouse compared to the original site on the abdomen. This adaptation of the grafted epidermis does not limit the usefulness of this animal model for dermatological research, when it is assessed by objective methods, allowing statistical comparison as described here.
    No preview · Article · Feb 1984 · Archives for Dermatological Research

  • No preview · Article · Feb 1984 · Progress in clinical and biological research
  • A Brüngger · M Hubler · H P Rohr

    No preview · Article · Feb 1984 · Experimental cell biology
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    ABSTRACT: Human benign prostatic hyperplasia is a predominantly stromal hyperplasia with accumulation of connective tissue. The main ultrastructural finding, which may be causal, is an activation of the smooth muscle cells, as seen by an increase of the volume density of the organelles within these cells. The dog is widely used as an animal model for human prostatic hyperplasia in spite of several differences. In this work the smooth muscle cells of the spontaneous and steroid-induced (by treating castrates with dihydrotestosterone and estradiol) prostatic hyperplasia of dogs were analysed by electron microscopical morphometry, and compared to estrogen or tamoxifen (antiestrogen) treated dogs as well as to untreated or castrated control dogs. The results clearly show that the prostatic smooth muscle cells of the dog can be activated by estrogen as well as tamoxifen, which proves the estrogenic side activity of the latter. In marked contrast to that, neither in the spontaneous nor in the steroid-induced prostatic hyperplasia could an activation of the smooth muscle cells be found. This is a most important difference from human benign prostatic hyperplasia, which limits the use of this animal model, and it might even be the explanation of the different reaction of human and canine prostatic hyperplasia to therapeutic hormonal manipulations.
    No preview · Article · Jan 1984 · The Journal of Urology
  • A. Brüngger · M. Hubler · H. P. Rohr

    No preview · Article · Jan 1984 · Pathobiology

Publication Stats

2k Citations
199.39 Total Impact Points

Institutions

  • 1975-2009
    • University of Innsbruck
      • Institute of Biochemistry
      Innsbruck, Tyrol, Austria
    • University of Leuven
      Louvain, Flanders, Belgium
  • 1969-1986
    • Universität Basel
      • • Institute of Geology and Paleontology
      • • Institut für Pathologie
      • • Zoological Institute
      Bâle, Basel-City, Switzerland
  • 1976
    • Harvard Medical School
      Boston, Massachusetts, United States
  • 1973
    • Pathologie Institut Enge
      Zürich, Zurich, Switzerland
  • 1967
    • University of Freiburg
      Freiburg, Baden-Württemberg, Germany