Hisayuki Takashima

Gifu University, Gihu, Gifu, Japan

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Publications (8)4.27 Total impact

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    ABSTRACT: Two Japanese Black bull calves from a dam showed muscular weakness and became recumbent after birth. At necropsy, skeletal muscles, including face, neck, body and proximal and distal forelimb and hindlimb were extremely pale in color and edematous. Histopathological examination of skeletal muscles revealed degenerative changes as follows: replacement of muscle with fat, variation in muscle fiber diameter, internal nuclei, central core-like structures, and vacuolar and hyaline degeneration of muscle fibers. Ultrastructurally, the lesions were characterized by focal myofibrillar disorganization with streaming or irregularity of Z bands. The present cases seem to be classified as congenital myopathy based on pathological alterations and age of onset.
    No preview · Article · Sep 2004 · Journal of Veterinary Medical Science
  • Hisayuki Takashima · Hiroki Sakai · Tokuma Yanai · Toshiaki Masegi
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    ABSTRACT: To detect serum antibody against Pasteurella multocida (P. multocida) in infected rabbits. a modified immunoperoxidase assay was applied. An outbreak of P. multocida infection in rabbits started from sudden death. The infected rabbits had severe fibrinous and purulent pneumonia with hemorrhage, and a large number of P. multocida (A:12) was isolated from the trachea and lungs of the animals. Antibodies of IgM and IgG to P. multocida were assessed by immunohistochemical staining using the sera of the animals as primary antibodies and applying them to formalin-fixed, paraffin-embedded sections of P. multocida attached to calf fibrin. IgM antibodies to P. multocida were first detected 7 days after the onset of the disease. IgG antibodies began to rise on the 7th or 14th day. These results suggested that the modified immunoperoxidase assay could detect antibodies against P. multocida.
    No preview · Article · Mar 2001 · Journal of Veterinary Medical Science
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    Hisayuki TAKASHIMA · Hiroshi TOMIMATSU · Toshiaki MASEGI
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    ABSTRACT: In February 1999, 5 of 11 fattening pigs in a litter on a farm in Gifu Prefecture demonstrated depression, coughing, and respiratory distress when 55 days old. Later the remaining 6 demonstrated the same symptoms. Five of the pigs died sporadically between the ages of 60 and 70 days. Necropsies showed typical pleuropneumonia in the thoracic cavity. Histopathological examination revealed severe fibrino-purulent pneumonia in 5 pigs.Actinobacillus pleuropneumoniaeserotype 1 was isolated in pure culture from the lungs of all the animals. Sera of the surviving 6 pigs were collected, and antibody responses againstA. pleuropneumoniae serotype1 isolated from the lungs of the dead pigs were examined by means of immuno-histochemical staining (IS) and the complement fixation (CF) test. IS-IgG antibodies were detected in 4 of 6 pig sera 4 days after the onset of the disease and in all sera 8 days after the onset. CF titers, on the other hand, were detected in 3 of 6 pig sera 4 days after the onset of the disease and in 4 of 6 pig sera 8 days after the onset. As a result of this study, we concluded that the IS test is more sensitive than the CF test and is a useful tool for the diagnosis of the incipient stage ofA. pleuropneumoniaeinfection.
    Preview · Article · Jan 2001
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    ABSTRACT: A total of 120 swine stomachs collected at a slaughterhouse in Gifu Prefecture was examined for the presence of Gastrospirillum-like organisms (GLO). All the pigs investigated had been slaughtered as healthy animals. Of 120 pigs, gastric ulcers were observed in 30 (25.0%) and erosion in 41 (34.2%). Of 21 pigs with spiral bacteria in the stomach mucosa, 8 (26.7%) demonstrated gastric ulcers, 9 (22.0%) erosion, 2 (9.5%) chronic gastritis, and 2 (7.1%) normal gastric mucosa. Ordinary bacteriological methods, however, cultured no GLO from any of the pigs. Spiral bacteria were found immersed in the mucous layer or inside gastric foveolae. Occasionally they were found in the lumens of gastric glands. The presence of GLO was seen as significantly associated (P<0.05) with the incidence of gastric ulcer and erosion.
    Preview · Article · Jan 2001
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    Hisayuki TAKASHIMA · Hiroshi TOMIMATSU
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    ABSTRACT: In 1998, 36% of all test sera from Gifu Prefecture contained an antibody against the porcine reproductive and respiratory syndrome (PRRS) virus. A retrospectively serological survey employing the enzyme-linked immunosorbent assay (ELISA) on sera collected from 1982 to 1998 showed that the antibody to PRRS virus was first detected in sera collected from fattening pigs in May 1988. Thereafter, the positive rate in Gifu Prefecture increased rapidly. No significant difference were observed between positive rates obtained with ELISA and those obtained from indirect immunofluorescence assay.
    Preview · Article · Jan 2000
  • H Takashima · H Sakai · T Yanai · T Masegi
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    ABSTRACT: Whole cells of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1, 2, 5 or 7 attached to fibrins were fixed in 10% neutral buffered formalin and embedded in paraffin. The sections on a slide glass were stained by the avidin-biotin complex immunoperoxidase (ABC) method. Test sera were applied to sections as primary antibodies. The serum antibodies against A.pleuropneumoniae (serotypes 1, 2, 5 and 7) were measured by the ABC method and complement fixation (CF) test. There was good correlation between the ABC and CF tests. The present results indicate that the immunohistochemical staining is as useful as the CF test for the detection and quantification of antibody in swine sera.
    No preview · Article · Jul 1999 · Journal of Veterinary Medical Science
  • Hisayuki Takashima · Hiroki Sakai · Tokuma Yanai · Toshiaki Masegi
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    ABSTRACT: Whole cells of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1, 2, 5 or 7 attached to fibrins were fixed in 10% neutral buffered formalin and embedded in paraffin. The sections on a slide glass were stained by the avidin-biotin complex immunoperoxidase (ABC) method. Test sera were applied to sections as primary antibodies. The serum antibodies against A.pleuropneumoniae (serotypes 1, 2, 5 and 7) were measured by the ABC method and complement fixation (CF) test. There was good correlation between the ABC and CF tests. The present results indicate that the immunohistochemical staining is as useful as the CF test for the detection and quantification of antibody in swine sera.
    No preview · Article · Jan 1999 · Journal of Veterinary Medical Science
  • T Yanai · C Matsumoto · H Takashima · K Yoshida · H Sakai · K Isowa · T Iwasaki · Y Sato · T Masegi
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    ABSTRACT: Bromodeoxyuridine (BrdU), a non-radioactive thymidine analogue, was administered to 15 cattle at a dosage of 1-10 mg/kg intravenously or intraperitoneally to demonstrate S-phase cells in the tissues. The organs and tissues were fixed in 10% neutral buffered formalin or in 70% ethanol, sectioned, denatured with hydrochloric acid, and treated with monoclonal antibody against BrdU. Immunohistochemical methods were used to "visualize" BrdU-labelled nuclei. BrdU-positive cells were satisfactorily demonstrated in both formalin- and ethanol-fixed tissues of animals given doses of 2 mg/kg or over, by either route of administration. Large numbers of BrdU-positive cells indicative of active cell production were found in the basal region of the stratified squamous epithelium, the neck between gastric pits and gastric glands in the abomasum, and the crypts of Lieberk├╝hn of the small and large intestines. Moderate numbers of positive cells were observed amongst inflammatory cells in cases of nephritis and in granulation tissue. Numerous positive cells were detected in leukaemia cells. The study showed that BrdU can be used to measure proliferative S-phase cells in cattle, as in human beings, mice and rats.
    No preview · Article · May 1996 · Journal of Comparative Pathology