H. Abe

Niigata University, Niahi-niigata, Niigata, Japan

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Publications (108)44.38 Total impact

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    ABSTRACT: Purpose: To report changes in microcirculation of optic nerve head after switching from topical latanoprost to tafluprost in eyes with glaucoma. Cases and Method: This study was made on 20 eyes of 20 patients treated by topical latanoprost for primary open-angle glaucoma. The age ranged from 43 to 78 years, average 66 years. Microcirculation in the optic nerve head was measured before, one and 3 months after switching to tafluprost. Laser speckle flowmetry was used in the measurement with mean blur rate serving as the indicator. Measurement was made on the optic nerve head as a whole and in each of 4 sectors. Results: There was no change in intraocular pressure, ocular perfusion pressure, systemic blood pressure, or pulse rate after switching. Microcirculation of optic nerve head increased significantly 3 months after switching (p=0.005-0.048 depending on the site of optic nerve head). Conclusion: Microcirculation in the optic nerve head increased significantly 3 months after switching from topical latanoprost to tafluprost in eyes with glaucoma.
    No preview · Article · Mar 2011
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    ABSTRACT: Purpose: To evaluate the change of follow-up intraocular pressure in the eyes with open-angle glaucoma (OAG) depending on improvement of glaucoma medication in Japan. Patients and methods: A total of 472 eyes from 279 OAG patients were enrolled into this study. Mean follow-up IOP of 1997 and 1998(1997/98) was compared with those for the 2 years between 2003 and recent. Additionally the number of eye drops and total dose frequency per day were compared between the 2 terms. Results: Follow-up IOP in OAG patients reduced from 15.3 ±2.79 mmHg to 14.0±2.71mmHg (8.63 %). The IOP reduction was significantly conspicuous in the medication group not in the group with filtering surgery. While the number of eye drops was similar but the dose frequency per day was reduced in POAG patients, the number of eye drops was greater but the dose frequency per day was almost similar in NTG cases. Conclusions : Recent glaucoma medication resulted in an increase in the efficiency for IOP treatments.
    No preview · Article · Mar 2011 · Folia Japonica de Ophthalmologica Clinica
  • K. Yaoeda · M. Shirakashi · H. Abe
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    ABSTRACT: Purpose : To report changes in the microcirculation in the optic nerve head following instillation of tafluprost in healthy humans. Cases and Method : This study was made on 9 eyes of 9 healthy males. The age ranged from 24 to 49 years, average 36 years. Microcirculation in and around the optic nerve head was measured using laser speckle flowgraphy before and after 7 days of instillation of 0.0015% tafluprost once daily, Results : Intraocular pressure decreased significantly after instillation of tafluprost (p=0.03). After 7 days of instillation, microcirculation increased significantly in the overall area of optic nerve head (p=0.008), superior to the disc (p=0.044), and temporal to the disc (D=0.044). Conclusion : Instillation of 0.0015% tafluprost for 7 days results in increased microcirculation in the optic nerve head.
    No preview · Article · Apr 2010
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    ABSTRACT: This study aimed to use adenoviral gene transfer to express matrix metalloproteinase (MMP)-2 in cultured porcine trabecular meshwork cells and to evaluate the duration of adenovirus-mediated MMP-2 expression and its enzymatic activity. MMP-2 cDNA was synthesized by ligating three segments of MMP-2 cDNA obtained by reverse transcription-polymerase chain reaction (RT-PCR) with mRNA extracted from mouse lungs. MMP-2 cDNA was inserted into replication-deficient adenoviral vectors. Western blotting revealed that MMP-2 was highly expressed by adenoviral gene transfer in cultured porcine trabecular meshwork cells. Zymography confirmed that the expressed MMP-2 possessed enzymatic activity and that MMP-2 activity increased dose-responsively with the viral titer. MMP-2 expression was detected two days after the additional virus preparation and continued for at least three weeks. Adenoviral vectors could efficiently deliver MMP-2 cDNA to cultured trabecular meshwork cells, with MMP-2 gene expression persisting for three weeks after infection. Our data have implications for future gene therapy in glaucoma.
    No preview · Article · Sep 2007
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    ABSTRACT: Purpose: To evaluate the results of mitomycin C (MMC) trabeculectomy combined with phacoemulsification and intraocular lens implantation (trabeculectomy triple) for patients with primary open-angle glaucoma (POAG) or normal-tension glaucoma (NTG). Methods: Trabeculectomy triple was performed in 31 eyes of 25 patients. The results were evaluated by Kaplan-Meier life table analysis. Trabeculectomy triple was considered to have failed if postoperative intraocular pressure (IOP) was decreased by less than 30% from the preoperative level. In addition, factors that could affect the outcome of surgery (patient age, sex, refraction, and diagnosis) were analyzed using the Cox proportional-hazards regression model. Results: The average±1 standard deviation for patient age was 74.3±5.89 years. The average preoperative IOP was 15.9±3.14mmHg, the average 1 year after surgery was 12.2±3.00mmHg, and the average 2 years postoperatively was 12.0±3.59mmHg. The probability of success (achievement of 30% or greater reduction in IOP) was 25.8% at 1 year. Higher patient age or a diagnosis of NTG was risk factors for less reduction in IOP. Conclusions: In this study, it was difficult using trabeculectomy triple to achieve more than 30% reduction in IOP. One possible reason was that many eyes had low IOP preoperatively. In cases of low preoperative IOP, trabeculectomy triple is less successful in lowering IOP.
    No preview · Article · Sep 2006 · Folia Japonica de Ophthalmologica Clinica
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    ABSTRACT: This study sought to elucidate the effects of timolol and dorzolamide on intraocular pressure (IOP) and retinal ganglion cell (RGC) death in an experimental model of glaucoma in rat. Mild elevation of IOP was induced in rats by intracameral injection of India ink and subsequent laser trabecular photocoagulation. IOP was measured before the surgical procedures and weekly thereafter. Timolol (0.5%), timolol XE (0.5%), dorzolamide (1%), and artificial tears (vehicle) were topically applied daily. Retinal sections were prepared for histology to determine RGC number. Timolol, timolol XE, and dorzolamide induced a significant reduction in IOP (p<0.05) and counteracted the reduction in RGC number that occurred in vehicle treated glaucomatous eyes (p<0.05). The coefficient of correlation between RGC number and IOP was significant in the dorzolamide treated group (r = -0.908, p<0.005), but not in other groups (p>0.05). Both timolol formulation and dorzolamide reduced IOP and protected RGCs in a rat model of experimental glaucoma. It cannot be ruled out that timolol might protect RGCs by additional mechanisms other than simply lowering of IOP.
    Full-text · Article · Apr 2005 · British Journal of Ophthalmology
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    ABSTRACT: Background: We report on a patient in whom unilateral Axenfeld-Rieger (A-R) syndrome was diagnosed and seclusion of the micropupil led to acute angle-closure glaucoma. Case Report: A 3-month-old baby with A-R syndrome in his right eye had seclusion of the right micropupil. The right eye also had acute corneal edema, loss of the anterior chamber, total angle closure, and increased intraocular pressure. On the basis of these findings we diagnosed acute angle-closure glaucoma. Ultrasound biomicroscopy of the right eye showed a tissue strand extending from the peripheral iris to a prominent Schwalbe's line and total closure of the angle due to total closure of the pupil. We performed pupilloplasty on the right eye with a vitreous cutter introduced through a side port placed at the corneal limbus. After pupilloplasty, the anterior chamber of the right eye was deeper and intraocular pressure in the right eye dropped to normal. Conclusion: Ophthalmologists must be prepared to manage an attack of acute angle-closure glaucoma in a patient with A-R syndrome if the patient has seclusion of the pupil or a micropupil.
    No preview · Article · Feb 2005 · Folia Japonica de Ophthalmologica Clinica
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    ABSTRACT: Background: Neovascular glaucoma due to internal carotid artery (ICA) occlusion is resistant to ophthalmic treatment and causes serious visual function loss. We report a case of neovascular glaucoma caused by ICA occlusion in which visual function was conserved for several years after diagnosis. Case Report: A 71-year-old male developed neovascular glaucoma in his left eye and bilateral retinal ischemia. The right ICA was completely occluded and the left ICA also had severe obstruction. He was treated with trabeculectomy followed by superficial temporal artery-middle cerebral artery anastomosis and carotid endarterectomy. After these surgical procedures, neovascularization disappeared and retinal ischemia improved. In spite of the severe ICA occlusion in this case, surgery resulted in conservation of this patient's visual acuity for 12 years until his death from unrelated causes. Conclusion: Ocular ischemic syndrome can be managed effectively by vascular reconstructive surgery to promote blood flow in addition to surgery to control intraocular pressure.
    No preview · Article · Nov 2004 · Folia Japonica de Ophthalmologica Clinica
  • T. Fukuchi · J. Ueda · H. Abe

    No preview · Article · Jan 2003
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    ABSTRACT: We measured the retinal nerve fiber layer thickness (NFLT) in 50 normal eyes and 50 glaucomatous eyes, using a Nerve Fiber Analyzer GDx (GDx). The glaucomatous eyes showed mean deviation of -5dB or greater by Humphrey 30-2 program. We used the following 11 parameters: symmetry, superior ratio, inferior ratio, superior/nasal, max. modulation, ellipse modulation, with the newly introducted parameters: normalized sup. area, normalized inf. area, elipse standard daviation, disciminant analysis, and sector analysis. When eyes were defined as abnormal if at least one parameter was abnormal, the sensitivity was 86% and the specificity was 54%. These findings show the potential usefulness of measurement of NELT using GDx with new parameters in the detection of early glaucoma.
    No preview · Article · Jan 2002
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    S Hasegawa · H Abe
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    ABSTRACT: To objectively measure a visual field and to sensitively detect glaucomatous visual field defects by measuring the contrast sensitivity function (CSF), using multifocal visual evoked cortical potentials (MVEPs). MVEPs of normal subjects (n = 28) and of patients with glaucoma (n = 12) or ocular hypertension (OH, n = 1) were recorded. A multi-input procedure was used to obtain 37 local VEP responses to each scaled hexagon, composed of 24 triangular patterns, reversing in a counterphase manner. Two pattern contrasts of 32% and 8% were used for measuring the contrast threshold. To improve the signal-to-noise ratio, 37 MVEPs were averaged into 20 groups. The root-mean-square (RMS) measures at both contrasts were calculated. Contrast thresholds were estimated by extrapolating the regression line of the amplitude versus contrast to the mean noise levels. RMS amplitudes of each local MVEP decreased as the eccentricity increased and as the pattern contrast decreased in normal subjects. It was also revealed that the amplitudes were smaller in the upper-half field than those in the lower-half field. Compared with the RMS amplitudes, CSFs estimated by MVEPs were relatively constant without being strongly influenced by retinal eccentricity. In patients with glaucoma, the CSFs, even from the locations where the mean perimetric sensitivities ranged to more than 30 dB, were significantly smaller than those in the normal control subjects (P < 0.001, Mann-Whitney test). CSFs in the 20- to 30-dB and 100 to 20-dB groups were also significantly smaller than those in the more-than-30-dB or 20- to 30-dB groups, respectively (P < 0.001, Mann-Whitney test). A significant correlation was found between the logarithmic function of the CSF and perimetric sensitivity (r = 0.57, P < 0.001, n = 216). The CSFs were evaluated on the basis of normal SD. Mappings of the CSFs agreed well with those of the perimetric sensitivity in all patients with glaucoma and was even more sensitive in detecting slight optic nerve damage by glaucoma than was perimetry. Local optic nerve damage caused by glaucoma can be sensitively detected by measuring contrast sensitivity using the MVEP.
    Preview · Article · Dec 2001 · Investigative Ophthalmology & Visual Science
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    ABSTRACT: We studied the control of intraocular pressure(IOP) by various types of blebs after non-penetrating trabeculectomy(NPT) and the difference between bleb formation after penetrating trabeculectomy(PT) and that after NPT. The filtering blebs of 45 yeys from 40 patients after NPT were studied using ultrasound biomicroscopy. They were grouped into four types, and the space under the scleral flap was classified into three types. The filtering blebs and the space under the scleral flap were correlated with IOP level. Overall, 40% of the blebs were L(low-reflective) type, 16% H(high-reflective) type, 16% E (encapsulated) type, and 29% F(flattened) type, but in good IOP control cases 59% of the blebs were L type, 14% H type, 14% E type, and 14% F type. L type blebs were found in 94% of eyes with good IOP control. Though filtering blebs of the L type could produce sufficient IOP reduction, blebs after NPT showed a greater tendency to become flattened than after PT. Additional systematic therapy must be designed to maintain the L type of filtering blebs after NPT.
    No preview · Article · Aug 2001 · Nippon Ganka Gakkai zasshi
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    T Fukuchi · J Ueda · H Abe · S Sawaguchi
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    ABSTRACT: The distribution of the cell adhesion glycoproteins, laminin, fibronectin, tenascin, vitronectin, thrombospondin, and entactin/nidogen, was examined in the human lamina cribrosa. Frozen sections of the optic nerve head from 7 normal human elderly donors were stained by immunohistochemistry. All six glycoproteins were detected in this tissue. While laminin and entactin/nidogen were observed linearly, reflecting the localization of basement membranes, fibronectin was identified diffusely. Marked tenascin immunoreactivity was apparent in the lamina cribrosa, but little or no tenascin staining was detected in the sclera. Vitronectin showed a fine fibrillar staining pattern in the lamina cribrosa, and, to a lesser extent, in the sclera and pial septa. Thrombospondin staining was apparent only in the sclera and the lamina cribrosa, which traversed the optic nerve. These results indicate that extracellular matrix components in the lamina cribrosa differ from those in the sclera or pial septa. This study is the first report that the human lamina cribrosa includes vitronectin and thrombospondin.
    Full-text · Article · Jul 2001 · Japanese Journal of Ophthalmology
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    ABSTRACT: To determine the role in the eye of chondromodulin (ChM)-I, which has been identified in cartilage as an angiogenic inhibitor, the expression and localization and a possible function of ChM-I were investigated. Expression and localization of ChM-I in rat eyes were examined by RNase protection assay and in situ hybridization and by immunostaining, using an antibody against a synthetic peptide. The effect of recombinant ChM-I on tube morphogenesis of retinal endothelial cells was examined in culture. The rat ChM-I gene was determined to encode the open reading frame of 334 amino acid residues, and ChM-I mRNA was exclusively expressed in cartilage, eye, and cerebellum in rats. ChM-I mRNA expression was evident in the iris-ciliary body, retina, and scleral compartments, but not in other compartments of the eye. In situ hybridization revealed mRNA expression in the ganglion cells, inner nuclear layer cells, and pigment epithelium in the retina and in the nonpigment epithelium of the ciliary body. Immunoreactive ChM-I was present in these cells and also in the vitreous body. Western blot analysis detected an approximately 25-kDa band of ChM-I presumed as a secretory form in the aqueous humor and vitreous body and an approximately 37-kDa band as a precursor form in the retina. Recombinant human ChM-I inhibited tube morphogenesis of human retinal endothelial cells in vitro. These observations indicate a potential role for ChM-I in inhibition of angiogenesis in the rat eye.
    Full-text · Article · Jun 2001 · Investigative Ophthalmology & Visual Science
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    T Fukuchi · J Ueda · T Hanyu · H Abe · S Sawaguchi
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    ABSTRACT: Remodeling of the extracellular matrix occurs in the lamina cribrosa in progressed glaucomatous optic nerve damage including disc cupping. We examined immunohistochemical changes in the transforming growth factor (TGF)-beta and platelet-derived growth factor (PDGF) in the optic nerve head in an experimentally induced glaucoma model. We used 3 cynomolgus and 2 Japanese monkey eyes. Glaucoma was induced by repeated argon laser photocoagulation of the chamber angle. Eyes were enucleated after disc cupping had formed 3 to 5 months after photocoagulation. The optic nerve head was examined for expression of TGF-beta1, -beta2 and -beta3 and PDGF-A and -B in frozen sections and by the biotin ExtraAvidin-alkali phosphatase method. Normal monkey eyes showed TGF-beta1, -beta2 and -beta3, and PDGF-A and -B in the optic nerve head including the nerve fibers, glial cells, and vascular cells. Glaucomatous eyes showed stronger expression of TGF-beta1 and -beta2 in the glial cells around the lamina cribrosa. The staining intensities for TGF-beta3, PDGF-A and -B were the same as in normal eyes. Eyes with experimental glaucoma showed higher expression of TGF-beta1 and -beta2 around the lamina cribrosa. These findings may show upregulation of extracellular matrix production as related to remodeling of the lamina cribrosa in glaucoma.
    Full-text · Article · Nov 2000 · Japanese Journal of Ophthalmology
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    ABSTRACT: Purpose: We attempted to analyze the three-dimensional ultrastructure of human corneal and scleral collagen fibrils with an atomic force microscope (AFM).Methods: A normal eye removed from a 66-year-old male was used in the study. Suspended corneal and scleral collagen fibrils were individually attached to glass slides by centrifugation. These collagen fibrils were air-dried and observed with a noncontact mode AFM in air.Results: AFM imaging provided information on the surface topography of both corneal and scleral collagen fibrils. The corneal collagen fibrils had a height of 11.9 +/- 1.0 (mean +/- standard deviation) nm and the scleral fibrils of 82.5 +/- 35.6 nm. A periodic banding pattern of grooves and ridges was clearly found in both types of fibrils: the D-periodicity and the groove depth were 65.7 +/- 0.8 nm and 1.46 +/- 0.50 nm in the corneal fibrils, and 67.3 +/- 1.1 nm and 6.16 +/- 1. 23 nm in the scleral fibrils.Conclusions: Surface topographic images of human corneal and scleral collagen fibrils were clearly obtained with the AFM. This technique provides quantitative information on the surface morphology of the collagen fibrils at high resolution.
    Preview · Article · Jun 2000 · Japanese Journal of Ophthalmology
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    ABSTRACT: Purpose: To evaluate the results of trabeculotomy for primary congenital glaucoma performed between 1982 and 1998 at Niigata University Hospital. Methods: The success rate of one or more trabeculotomies performed before 3 years of age was evaluated by Kaplan-Meier analysis of results for 54 eyes with congenital or early-onset glaucoma (diagnosis before 3 months of age, n=28) compared to late-onset glaucoma (diagnosis after 3 months of age, n=26). Results: The 54 eyes underwent 1 to 3 (mean 1.67± 0.6) trabeculotomies. The follow-up periods after trabeculotomy ranged from 6 months to 16.7 years (mean 91.3± 59.7 months). The mean intraocular pressure was 24.8±7.5 mmHg before surgery. The probable success rate of 1 or more trabeculotomies was 70.1% at 1 year and 62.7% at 5 years. The probability of success was significantly lower (p< 0.01) for the 28 eyes with early-onset glaucoma compared to the 26 eyes with late-onset glaucoma. Conclusions: Trabeculotomy is a clinically successful treatment for primary congenital glaucoma; however, the operation has less probability of success in patients with early-onset glaucoma (diagnosis before 3 months of age).
    No preview · Article · Jan 2000 · Folia Japonica de Ophthalmologica Clinica
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    ABSTRACT: A 74-year-old female presented with suprachoroidal hemorrhage about 3 months after combined glaucoma and cataract surgery including trabeculectomy with mitomycin C (MMC), extracapsular cataract extraction (ECEE), and intraocular lens (IOL) implantation in the left eye. She complained of sudden loss of vision, and examination showed "kissing" ciliochoroidal detachment, visual acuity of light perception only, and intraocular pressure of 10 mmHg. She underwent posterior sclerotomy, during which bloody suprachoroidal fluid was drained. On the first day after fluid drainage, ciliochoroidal detachment resolved and by about 2 months postoperatively, her visual acuity had improved to 20/20, almost the same as before suprachoroidal hemorrhage. Generally, suprachoroidal hemorrhage occurs during or within a few days after intraocular surgery. However, ophthalmologists should watch for delayed suprachoroidal hemorrhage in cases of combined glaucoma and cataract surgery, particularly those including trabeculectomy, ECCE, and IOL implantation.
    No preview · Article · Jan 2000 · Folia Japonica de Ophthalmologica Clinica
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    ABSTRACT: We attempted to analyze the three-dimensional ultrastructure of human corneal and scleral collagen fibrils with an atomic force microscope (AFM). A normal eye removed from a 66-year-old male was used in the study. Suspended corneal and scleral collagen fibrils were individually attached to glass slides by centrifugation. These collagen fibrils were air-dried and observed with a non-contact mode AFM in air. AFM imaging provided information on the surface topography of both corneal and scleral collagen fibrils. The corneal collagen fibrils had a height of 11.9 +/- 1.0 (mean +/- standard deviation) nm and the scleral fibrils of 82.5 +/- 35.6 nm. A periodic banding pattern of grooves and ridges was clearly found in both types of fibrils; the D-periodicity and the groove depth were 65.7 +/- 0.8 nm and 1.46 +/- 0.50 nm in the corneal fibrils, and 67.3 +/- 1.1 nm and 6.16 +/- 1.23 nm in the scleral fibrils. Surface topographic images of human corneal and scleral collagen fibrils were clearly obtained with the AFM. This technique provides quantitative information on the surface morphology of the collagen fibrils at high resolution.
    No preview · Article · Dec 1999 · Nippon Ganka Gakkai zasshi
  • T Fukuchi · J Ueda · T Hanyu · H Abe · S Sawaguchi
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    ABSTRACT: Remodeling of the extracellular matrix occurs in the lamina cribrosa in progressed glaucomatous optic nerve damage including disc cupping. We examined immunohistochemical changes in the transforming growth factor (TGF)-beta and platelet derived growth factor (PDGF) in the optic nerve heads in experimentally induced glaucoma. We used 3 cynomolgus and 2 Japanese monkey eyes. Glaucoma was induced by repeated argon laser photocoagulation of the chamber angle. Eyes were enucleated after disc cupping had formed 3 to 5 months after treatment. The optic nerve head was examined for expression of TGF beta 1, beta 2, and beta 3, and PDGF A and B in frozen sections and by the biotin-ExtrAvidin-Alkali Phosphatase method. Normal monkey eyes showed TGF beta 1, beta 2, and beta 3, and PDGF A, and B in the optic nerve head including the nerve fibers, glial cells, and vascular cells. Glaucomatous eyes showed stronger expression of TGF beta 1 and beta 2 in the glial cells around the lamina cribrosa. The staining intensities for TGF beta 3, PDGF A, and PDGF B were the same as in normal eyes. Eyes with experimental glaucoma showed higher expressions of TGF beta 1 and beta 2 around the lamina cribrosa. This finding may show upregulation of extracellular matrix production as related to remodeling of the lamina cribrosa in glaucoma.
    No preview · Article · Apr 1999 · Nippon Ganka Gakkai zasshi

Publication Stats

716 Citations
44.38 Total Impact Points

Institutions

  • 1991-2005
    • Niigata University
      • • Division of Ophthamology and Visual Sciences
      • • School of Medicine
      Niahi-niigata, Niigata, Japan
  • 1997
    • Kyoto University
      • Department of Ophthalmology and Visual Sciences
      Kioto, Kyōto, Japan
  • 1990-1996
    • St. Marianna University School of Medicine
      • Department of Cardiovascular Surgery
      Kawasaki, Kanagawa-ken, Japan