M.-C. Hennion

École Supérieure de Physique et de Chimie Industrielles, Lutetia Parisorum, Île-de-France, France

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Publications (81)237.04 Total impact

  • V. Pichon · F. Chapuis-Hugon · M.-C. Hennion
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    ABSTRACT: To improve selectivity during sample pretreatment, various biomolecular tools can be immobilized on a solid phase by covalent and noncovalent immobilization procedures and applied as extraction sorbents for the treatment of complex samples. These bioaffinity sorbents can be prepared using, for example, antibodies or aptamers, thus giving rise to a retention mechanism during the extraction based on molecular recognition. Due to the very high affinity and high selectivity of these biomolecules for target compounds, they have been shown to be a unique tool in the sampling field. Other types of biological molecules presenting high affinity for a particular part of a molecule such as protein A/G, lectins, enzymes, as well as molecular receptors sensitive to molecular interactions with certain groups of molecules, can also be used as selective tools in sample extraction procedures. This chapter describes the development and the properties of these different types of bioaffinity sorbents (immobilization procedure, capacity, recoveries of extraction, stability, reusability, etc.) for the treatment of complex samples. Their association with analytical devices, from conventional to miniaturized analytical systems, is also discussed.
    No preview · Article · Dec 2012
  • M.-C. Hennion · P. Gareil · A. Hagège · A. Kuhn · V. Pichon
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    ABSTRACT: Analytical chemistry is strongly involved in sustainable development and several aspects ot tne actual research are described in this paper. For trace and ultra-trace analysis in complex samples, new sorbents have been synthesized for a high selective extraction and concentration of analytes involving interaction based on the use of antibodies, aptamers or imprinted polymers. The toxicity of a given sample depends on its concentration and on its speciation. Therefore it is important to characterize the complex between metals and proteins, which can be achieved by new devices coupling zone electrophoresis separation and ICP/MS detection. In order to achieve rapid separations from small size samples, new trends are given in electrokinetic separations coupled to in-capillary separation and MS are described, as well as in the area of microsystems on chips. The last part describes the trends in bioelectrochemical biosensors with the development of macroporous materials with a controlled architecture.
    No preview · Article · Feb 2010
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    Florence Chapuis · Valérie Pichon · M.-C. Hennion
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    ABSTRACT: Cet article a pour objectif de présenter les caractéristiques de l'extraction en phase solide pour les analyses environnementales à partir de matrices ou d'extraits liquides. Les principes fondamentaux qui régissent l'extraction sont expliqués afin de pouvoir choisir la nature et la quantité de phase extractante selon la matrice des échantillons et les propriétés des solutés recherchés en s'appuyant sur les mécanismes de rétention des phases. Les derniers développements concernant les formats disponibles, la nature des phases et l'automatisation sont présentés. Un intérêt particulier est porté aux phases sélectives émergentes, notamment celles qui agissent par reconnaissance moléculaire telles les immunoadsorbants ou les polymères à empreinte moléculaire permettant en une seule étape d'avoir un extrait propre. Les performances de leur couplage en ligne avec la séparation par chromatographie en phase liquide sont illustrées par des exemples dans le domaine environnemental. Quelques exemples particuliers à l'analyse des produits pétroliers sont également présentés.
    Full-text · Article · Nov 2005 · Oil & Gas Science and Technology
  • V. Pichon · F. Chapuis · M.-C. Hennion
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    ABSTRACT: The trace-level determination of micropollutants in environmental matrices requires high performance from analytical separation and detection as well as from the sample preparation technique. Low detection limits are difficult to obtain owing to many interfering compounds co-eluted with the target analytes because they are co-extracted by non-selective reversed-phase sorbents used for the solid-phase extraction of aqueous samples or by organic solvents used for the treatment of solid matrices. Immunoaffinity sorbents based on the use of antibodies that are specific of the target analytes can be applied to the direct extraction of a group of structurally related pollutants from liquid samples or to the purification of extracts (resulting of solvent based extraction methods applied to solid matrices). The high selectivity of the antigen-antibody interaction allows reaching the detection limits according to the regulatory guidelines in the various studied matrices. However, the production of antibodies is time consuming and cost effective. An alternative SPE method is emerging that consists in synthesizing highly cross-linked polymers that possess cavities that are specific of a particular molecule. These molecularly imprinted polymers possess properties that can be directly compared to those of immunosorbents with the advantage of being very stable and less cost effective.
    No preview · Article · Aug 2004 · L'Actualité chimique
  • Valérie Pichon · AI Krasnova · M.-C. Hennion
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    ABSTRACT: An immunoaffinity solid-phase-extraction sorbent has been developed by raising polyclonal antibodies against propanil and covalently bonding them to activated silica. This immunosorbent (IS) had high affinity for propanil and for structurally related phenylurea herbicides whereas structurally related chloroanilines were retained only poorly. Studies of breakthrough volumes, capacity, and calibration ranges of selected pesticides showed that the antibodies have different affinities for each compound. Propanil and phenylureas could be quantitatively recovered by elution with methanol–water; average recoveries were close to 100%. The IS was also packed in a precolumn and coupled on-line to HPLC–UV. The high selectivity provided by the IS was demonstrated by comparing results with those obtained by use of a non-selective polymeric sorbent when both were used for direct extraction of propanil and phenylureas from a 50-mL sample of river-Seine water spiked at 0.1 g L–1 or from lemon juice spiked at 50 g L–1.
    No preview · Article · Jan 2004 · Chromatographia
  • C Miège · J Dugay · M C Hennion
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    ABSTRACT: There is a need for a better characterization of sludges from wastewater treatment plants which are destined to be spread on agricultural lands. Inorganic pollutants are regularly controlled but organic pollutants have received few attention up to now. On this paper, we have been interested on the analysis of the 16 polycyclic aromatic hydrocarbons (PAHs) listed in the US Environmental Protection Agency (US EPA) priority list and more particularly of the six PAHs listed in the European community list (fluoranthene, benzo[b and k]fluoranthene, benzo[a]pyrene, benzo[ghi]perylene, indeno[1,2,3-cd]pyrene). The analysis step consists on liquid chromatography with both fluorescence and UV detections as described in the EPA Method 8310. As for the extraction step, several techniques such as supercritical fluid extraction, pressurized liquid extraction, focused microwave extraction in open vessels, Soxhlet and ultrasonic extractions are compared after optimization of the experimental conditions (solvent nature and quantity, temperature, pressure, duration, ... ) and validation with certified sludges. When optimized, these five extraction techniques are as much efficient with similar relative standard deviation. Whatever the extraction techniques used, the whole analysis protocol permits to quantify PAHs in the range of 0.09 to 0.9 mg/kg of dried sludges.
    No preview · Article · Jun 2003 · Journal of Chromatography A
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    ABSTRACT: Direct, selective solid-phase extraction of triazines from aqueous samples is presented using a molecularly imprinted polymer (MIP) made with terbutylazine as template molecule. After optimization of the steps of the procedure, 14 triazines including degradation products were studied and satisfactory extraction recoveries were obtained except for thiotriazines. By comparing results obtained with the terbutylazine MIP and a similar non-imprinted polymer, it was determined that retention was achieved via specific interactions except for hydroxyterbutylazine. Selectivity of the extraction procedure was also verified by applying the MIP for the extraction of phenylureas that were not retained on it. The effects of the charge distribution and of molecular volume of the triazines (obtained by molecular modeling) on the selectivity of interactions between the analytes and the MIP were studied. However, when the optimized procedure was applied to real samples, low extraction recoveries were obtained due to strong matrix effects: ion-exchange occurs between the carboxylate groups of the MIP and the ionic species of the sample, that prevents subsequent specific interactions. By introducing an acid wash step, the procedure was successfully applied for the class-selective extraction of triazines from industrial effluent and surface water samples. Finally, increased extraction recoveries were achieved for the polar degradation products of triazines by using a mixed-phase composed of a polymeric sorbent and the MIP.
    No preview · Article · Jun 2003 · Journal of Chromatography A
  • V Pichon · MC Hennion

    No preview · Article · May 2003 · Journal of Chromatography A
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    ABSTRACT: Phenolic compounds present in olive mill wastewater (OMW) need to be quantified because of their pollution capacity toward the environment. In the present study, six representative phenolic compounds of OMW were chosen to develop a LC-MS method. The high polarity of the compounds caused problems when using traditional reversed-phase liquid chromatography (RPLC). Consequently, a method was developed on another kind of chromatographic phase: Porous Graphitic Carbon (PGC) involving the use of a tetrahydrofuran (THF) gradient. The influence of THF as mobile phase in LC-MS coupling, which is not common practice, was evaluated. In Atmosperic Pressure Chemical Ionisation (APCI) in the negative ion mode, the presence of THF in the mobile phase did not degrade the MS signal of the target compounds in the conditions studied. On the contrary, an improvement was even observed when the percentage of THF increased. The proposed PGC-LC-MS method was selective and linear for the six phenolic compounds analysed with limits of quantification lower than 5 ppm in all cases. The precision was satisfactory (pooled RSD around 6%). The analyses of OMW matrix spiked sample confirmed the good performance of the proposed method.
    Full-text · Article · Jan 2002 · Journal of Chromatography A
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    Preview · Article · Jan 2002
  • S Nicol · J Dugay · MC Hennion
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    ABSTRACT: PAHs and their nitrated derivatives (Nitro-PAHs) have been analyzed in extracts from particulate matter by GC-MS and GC-MS-MS. Using GC-MS, most of these compounds could not be detected in raw extracts or their confirmation was uncertain because of interfering compounds from the matrix. Detection and quantification was greatly improved by using GC-MS-MS. Limits of detection were lower than 40 pg (injected) in standard solution and in diesel particulate matter extracts (for 10 mg of particles extracted). Low traces of PAHs and Nitro-PAHs, in the range of a few pg . m(-3), could be detected without purification or fractionation of the extract.
    No preview · Article · Jul 2001 · Journal of Separation Science
  • Sophie Nicol · José Dugay · Marie‐Claire Hennion
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    ABSTRACT: PAHs and their nitrated derivatives (Nitro-PAHs) have been analyzed in extracts from particulate matter by GC-MS and GC-MS-MS. Using GC-MS, most of these compounds could not be detected in raw extracts or their confirmation was uncertain because of interfering compounds from the matrix. Detection and quantification was greatly improved by using GC-MS-MS. Limits of detection were lower than 40 pg (injected) in standard solution and in diesel particulate matter extracts (for 10 mg of particles extracted). Low traces of PAHs and Nitro-PAHs, in the range of a few pg·m–3, could be detected without purification or fractionation of the extract.
    No preview · Article · Jun 2001 · Journal of Separation Science
  • L Malleret · † and A. Bruchet · M C Hennion
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    ABSTRACT: "Earthy-musty" off-flavor problems in water samples are due to organic compounds present at the sub-part-per-trillion level. Numerous analytical methods such as purge and trap, liquid/liquid extraction, and closed-loop stripping analysis (CLSA) followed by GC/MS analysis have been used to determine these compounds. However, these methods offer poor sensitivity (detection limits of approximately 1 to 10 ng/L) when compared to the 20-30 pg/L of sensorial sensitivity. The purpose of this study was to develop a new method involving a modified CLSA preconcentration technique together with large volume injection GC/MS in order to attain analytical sensitivity equal to or better than olfactory sensitivity. For eight target compounds that cause taste and odor problems in water at trace levels, the method developed was linear in the 0.05-10 ng/L range and provided recoveries greater than 70% together with satisfactory repeatability. Detection limits as low as 15-30 pg/L were achieved, representing a 50-fold improvement in sensitivity as compared to current methods. The accuracy and sensitivity of the method were demonstrated in different aqueous matrixes, including raw surface water. The method was successfully applied to earthy-musty water samples that had remained unsolved by conventional techniques, thus proving its effectiveness.
    No preview · Article · May 2001 · Analytical Chemistry
  • N. Delaunay-Bertoncini · V. Pichon · M.-C. Hennion
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    ABSTRACT: A description of immunosorbents, which are solid-phase extraction sorbents based on molecular recognition using antibodies, is provided. The sysntesis of immunosorbents for small molecules is discussed as are the main parameters governing their extraction. The high selectivity of these reagents is demonstrated in a number of applications.
    No preview · Article · Jan 2001
  • S. Nicol · J. Dugay · M.-C. Hennion

    No preview · Article · Jan 2001
  • N. Delaunay-Bertoncini · V. Pichon · M. -C. Hennion
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    ABSTRACT: Adsorbents involving antigen-antibody interactions, so-called immunosorbents, have high affinity and class-selective extraction properties. Although most studies of immunosorbents are based on the use of polyclonal antibodies, to ensure a good repeatability with this kind of sorbent monoclonal antibodies are required. This study shows that the cross-reactivity of monoclonal anti-isoproturon antibodies is similar to that of polyclonal anti-isoproturon antibodies. To reduce the price of the resulting immunosorbent, the bonding density has been optimized. The choice of activated silica rather than Sepharose as solid support is discussed. The effect of sample volume and of the amount of immunosorbent on extraction recoveries for some phenylureas has been studied. It appears that the amount of immunosorbent can be readily adapted to the detection limit required.
    No preview · Article · Dec 2000 · Chromatographia
  • S. Nicol · J. Dugay · M. -C. Hennion
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    ABSTRACT: O-PAHs have been analyzed in extracts of particulate matter by GC-MS-MS and GC-MS. Using GC-MS, most O-PAHs could not be detected in raw extracts, or their confirmation was uncertain due to interfering compounds from the matrix. Detection and quantification was greatly improved by using GC-MS-MS. Linearity was observed in the range from 10 to 1000 pg injected. Low traces of O-PAHs, in the range of a few pg·m−3 of air, could be detected without purification or fractionation of the extract.
    No preview · Article · Dec 2000 · Chromatographia
  • M. C. Hennion

    No preview · Chapter · Dec 2000
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    ABSTRACT: An on-line technique coupling preconcentration via a microprecolumn with microcolumn-liquid chromatography (micro-LC) on a 1-mm inner diameter (i.d.) analytical column was investigated. The system was found to be very simple, fast, and efficient for the trace-level determination of some natural hepatotoxins synthesized by Cyanobacteria, namely, microcystins, in environmental water. Particular attention was paid to band broadening, and a good quality coupling was obtained. A cleanup was introduced in the solid-phase extraction (SPE) sequence to eliminate part of the interferents co- extracted during the sample percolation. The technique was reproducible, with relative standard deviations ranging from 3 to 8%, linear in the range 0.05–50 μg/L. Microcystins could be detected below the 0.04 μg/L level in drinking and surface water using only 5-mL sample volumes, with UV detection. The very small sample volume, resulting from lower detection limits, in absolute amounts, obtained in micro-LC represents a definite advantage over on-line classical chromatography. The suitability of on-line micro-SPE–micro-LC–MS was also evaluated. Application to the determination of microcystins in environmental and drinking water samples are presented. © 2000 John Wiley & Sons, Inc. J Micro Sep 12: 323–332, 2000
    No preview · Article · May 2000 · Journal of Microcolumn Separations
  • C Miège · M Bouzige · S Nicol · J Dugay · V Pichon · M.C. Hennion
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    ABSTRACT: A selective clean-up procedure using immunoaffinity solid-phase extraction was applied for the trace-level determination of polycyclic aromatic hydrocarbons (PAHs) in urban waste water and sewage sludges used for soil amendment. Anti-pyrene antibodies have been immobilized on a silica-based sorbent and the cross-reactivity of the antibodies towards structurally related compounds were allowed to extract the whole class of priority PAHs. The selectivity of the antibodies provided clean extracts from sludges and, therefore, the identification and quantification were shown to be easier using either liquid chromatography (LC) with UV diode array and fluorescence detection in series or gas chromatography-mass spectrometry (GC-MS), although some loss of up to 50% was observed for the clean-up. The identification of the PAHs by matching of UV and MS spectra was greatly improved. The procedure, including immunoclean-up and LC coupled to diode array and fluorescence detection, was validated using certified reference materials with native PAHs of concentrations in the range of 0.57-2.16 mg/kg (dry sludges).
    No preview · Article · Nov 1999 · Journal of Chromatography A