D Rajasekaran

Annamalai University, Anamalainagar, Tamil Nadu, India

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Publications (21)11.61 Total impact

  • D Rajasekaran · S Manoharan · M M Prabhakar · A Manimaran
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    ABSTRACT: Oral cancer is one of the most common malignancies worldwide, and India has recorded the highest annual incidence of oral cancer in comparison with other countries. Altered lipid peroxidation and antioxidant status along with defect in detoxification cascade have been implicated in the pathogenesis of several cancers including oral cancer. The aim of this study was to investigate the chemopreventive potential of ethanolic extract of Enicostemma littorale leaves (ElELet) in 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. Oral tumor was developed in the buccal pouches of male golden Syrian hamsters by painting with 0.5% DMBA three times a week for 14 weeks. We observed 100% tumor formation with increase in tumor volume and tumor burden in the hamsters treated with DMBA alone. Imbalance in phase I (cytochrome P450 and cytochrome b5) and phase II (glutathione reductase, glutathione-S-transferase, glutathione, and Deoxythymidine-diaphorase (DT)-diaphorase) detoxification agents and lipid peroxidation by-products (thiobarbituric acid reactive substances) and antioxidant (superoxide dismutase, catalase, glutathione peroxidase, and vitamins E and C) status was noticed in hamsters treated with DMBA alone. Oral administration of ElELet at a dose of 250 mg/kg body weight to hamsters treated with DMBA significantly prevented both precancerous and cancerous lesions in the oral cavity. ElELet modulated the status of phase I and II detoxification agents and antioxidants in favor of the suppression of oral carcinogenesis. This study thus suggests that E. littorale might have inhibited the oral carcinogenesis in DMBA-treated hamsters through its antioxidant potential. The present findings are also substantiated by histological studies during DMBA-induced oral carcinogenesis. © The Author(s) 2014.
    No preview · Article · Sep 2015 · Human & Experimental Toxicology
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    ABSTRACT: Enicostemma littorale leaves are traditionally used for the treatment of several diseases, including inflammation and cancer. This study has taken effort to explore the antitumor initiating potential of E. littorale leaves (ElELet) by analyzing the expression pattern of apoptotic (p53, Bcl-2 and Bcl-2 associated X-protein), cell-proliferative (cyclin D1 and proliferating cell nuclear antigen), angiogenic (vascular endothelial growth factor), invasive (matrix metalloproteinase-2 and 9), and inflammatory (NF-κB and cyclooxygenase-2) markers during 7, 12-dimethylbenz (a) anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Oral tumors were induced in the buccal pouches of hamsters using the potent site and organ specific carcinogen, DMBA. DMBA application 3 times a week for 14 weeks resulted in tumor formation in the buccal pouches. Hundred percent tumor formations with dysregulation in the expression pattern of apoptotic, cell proliferative, inflammatory, angiogenic, and invasive markers were observed in the buccal pouches of hamsters treated with DMBA alone. ElELet at a dose of 250 mg/kg body weight orally to DMBA treated hamsters significantly prevented the tumor formation as well as corrected the abnormalities in the expression pattern of above mentioned molecular markers. ElELet thus modulated the expression pattern of all the above mentioned molecular markers in favor of the suppression of cell proliferation occurring in DMBA induced hamster buccal pouch carcinogenesis.
    No preview · Article · Jan 2015 · Toxicology International
  • D. Rajasekaran · R. Kowsalya · R. Selvasundaram · S. Manoharan
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    ABSTRACT: Glycoconjugates [protein bound hexose, protein bound hexosamine, total sialic acid and fucose] play a pivotal role in neoplastic transformation if they are abnormally expressed. The protective effect of Enicostemma littorale leaves on cell surface abnormalities was analyzed by measuring the status of the above said glycoconjugates during 7,12-dimethylbenz(a) anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Tumors were developed in the buccal mucosa of hamsters by painting with DMBA thrice a week for 14 weeks. Elevated levels of glycoconjugates were observed in both plasma and tumor tissues of hamsters treated with DMBA alone. Oral administration of Enicostemma littorale leaf extract at a dose of 250 mg/kg bw to hamsters treated with DMBA significantly brought back the levels of glycoconjugates to near normal range. Enicostemma littorale leaves, thus have the potential to prevent the abnormalities in the glycoconjugates status occurring in DMBA induced oral carcinogenesis.
    No preview · Article · Jan 2015 · International Journal of Pharma and Bio Sciences
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    Dataset: O&P-adp
    D Palanimuthu · N Baskaran · S Silvan · D Rajasekaran · S Manoharan

    Full-text · Dataset · Feb 2014
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    Dataset: jalal-adp
    D Palanimuthu · N Baskaran · S Silvan · D Rajasekaran · S Manoharan

    Full-text · Dataset · Feb 2014
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    Dataset: JCTR- adp
    D Palanimuthu · N Baskaran · S Silvan · D Rajasekaran · S Manoharan

    Full-text · Dataset · Feb 2014
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    Dataset: IJPSR-adp
    D Palanimuthu · N Baskaran · S Silvan · D Rajasekaran · S Manoharan

    Full-text · Dataset · Feb 2014
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    Dataset: bas-adp I
    D Palanimuthu · N Baskaran · S Silvan · D Rajasekaran · S Manoharan

    Full-text · Dataset · Feb 2014
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    Dataset: kow-adp
    D Palanimuthu · N Baskaran · S Silvan · D Rajasekaran · S Manoharan

    Full-text · Dataset · Feb 2014
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    Dataset: IJRPS-adp
    D Palanimuthu · N Baskaran · S Silvan · D Rajasekaran · S Manoharan

    Full-text · Dataset · Feb 2014
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    Dataset: bas-adp
    D Palanimuthu · N Baskaran · S Silvan · D Rajasekaran · S Manoharan

    Full-text · Dataset · Feb 2014
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    Dataset: raj-adp
    D Palanimuthu · N Baskaran · S Silvan · D Rajasekaran · S Manoharan

    Full-text · Dataset · Feb 2014
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    ABSTRACT: Our aim was to explore anti-cell proliferative and anti-angiogenic potential of andrographolide by analyzing the expression pattern of cell proliferative (PCNA, Cyclin D1) and angiogenic (VEGF) markers during 7, 12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch carcinogenesis. DMBA painting three times a week for 14 weeks in the buccal pouch of golden Syrian hamsters resulted in oral tumors which were histopathologically diagnosed as well differentiated squamous cell carcinoma. Immunohistochemical (PCNA, VEGF) and RT-PCR (Cyclin D1) studies revealed over expression of PCNA, VEGF and Cyclin D1 in the buccal mucosa of hamsters treated with DMBA alone. Oral administration of andrographolide at a dose of 50 mg/kg bw to hamsters treated with DMBA not only suppressed the histological abnormalities but also down regulated the expression of PCNA, VEGF and Cyclin D1. The results of the present study suggest that andrographolide suppressed tumor formation in the buccal mucosa of hamsters treated with DMBA through its anti-cell proliferative and anti-angiogenic potential.
    Full-text · Article · Oct 2013 · Asian Pacific journal of cancer prevention: APJCP
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    ABSTRACT: Our aim was to investigate the chemopreventive potential of saffron in DMBA-induced hamster buccal pouch carcinogenesis. Assessment was by monitoring the percentage of tumor bearing hamsters, tumor size as well as the status of detoxification agents, lipid peroxidation and antioxidants. Oral squamous cell carcinomas were induced in the buccal pouch of Syrian golden hamsters by painting them with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. We observed 100% oral tumor formation with severe histopathological abnormalities in all the hamsters treated with DMBA alone, activities of phase I and phase II detoxification enzymes, lipid peroxidation and antioxidants being significantly altered. Though oral administration of saffron completely prevented the formation of tumors, we noticed severe hyperplasia and dysplasia in hamsters treated with DMBA, suggesting that tumors might eventually develop. Oral administration of saffron return detoxification enzymes, lipid peroxidation and antioxidants to normal ranges. The chemopreventive potential of saffron thus is likely due to antioxidant properties and modulating effects on detoxification in favour of the excretion of carcinogenic metabolites during DMBA-induced hamster buccal pouch carcinogenesis.
    No preview · Article · Feb 2013 · Asian Pacific journal of cancer prevention: APJCP
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    ABSTRACT: The present study has investigated the modulating effect of carnosic acid on the expression pattern of cell proliferative (proliferating cell nuclear antigen (PCNA) cyclin D1 and a transcription factor c-fos), apoptotic (p53, Bcl-2, Bax caspase -3 and 9), inflammatory (Nuclear factor kappa B (NFκB) and cyclooxygenase-2 (COX- 2) and angiogenic (vascular endothelial growth factor (VEGF) markers during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. Oral tumors were developed in the hamsters buccal pouches by painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. Hundred per cent tumour formation (well-differentiated squamous cell carcinoma) accompanied by deregulation in the above mentioned molecular markers was noticed in hamsters treated with DMBA alone (tumour bearing hamsters). Oral administration of carnosic acid at dose of 10mg/kg bw to hamsters treated with DMBA not only completely prevented the tumour formation, but also corrected the abnormalities in the expression pattern of molecular markers. The present study suggests that carnosic acid might have inhibited the tumour formation by exerting anti-cell-proliferative, anti-inflammatory, anti-angiogenic and apoptotic potential during DMBA-induced hamster buccal pouch carcinogenesis.
    Preview · Article · Oct 2012 · African Journal of Traditional, Complementary and Alternative Medicines
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    D Palanimuthu · N Baskaran · S Silvan · D Rajasekaran · S Manoharan
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    ABSTRACT: The oral cancer chemopreventive efficacy of lupeol, a bioactive triterpene, was assessed by monitoring the tumor incidence and using the status of phase I and II xenobiotic metabolizing enzymes, lipid peroxidation and antioxidants as biochemical end points during 7,12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Oral tumors were developed in the buccal pouch of golden Syrian hamsters by painting with 0.5 % DMBA three times a week for 14 weeks. Well differentiated oral squamous cell carcinoma with marked abnormalities in the status of biochemical markers were noticed in hamsters treated with DMBA alone. Oral administration of lupeol at a dose of 50 mg/kg bw completely inhibited the formation of oral tumors and restored the status of biochemical markers during DMBA induced oral carcinogenesis. The present study thus demonstrates the chemopreventive potential of lupeol in DMBA induced oral carcinogenesis. The chemopreventive potential of lupeol is probably due to its antioxidant or free radical scavenging property and modulating effect on phase I and II xenobiotic metabolizing enzymes in favour of the excretion of carcinogenic metabolites during DMBA induced hamster buccal pouch carcinogenesis.
    Full-text · Article · Jul 2012 · Pathology & Oncology Research
  • Palanimuthu D · Baskaran N · Silvan S · Rajasekaran D · Manoharan S
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    ABSTRACT: Abstract The oral cancer chemopreventive efficacy of lupeol, a bioactive triterpene, was assessed by monitoring the tumor incidence and using the status of phase I and II xenobiotic metabolizing enzymes, lipid peroxidation and antioxidants as biochemical end points during 7,12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Oral tumors were developed in the buccal pouch of golden Syrian hamsters by painting with 0.5 % DMBA three times a week for 14 weeks. Well differentiated oral squamous cell carcinoma with marked abnormalities in the status of biochemical markers were noticed in hamsters treated with DMBA alone. Oral administration of lupeol at a dose of 50 mg/kg bw completely inhibited the formation of oral tumors and restored the status of biochemical markers during DMBA induced oral carcinogenesis. The present study thus demonstrates the chemopreventive potential of lupeol in DMBA induced oral carcinogenesis. The chemopreventive potential of lupeol is probably due to its antioxidant or free radical scavenging property and modulating effect on phase I and II xenobiotic metabolizing enzymes in favour of the excretion of carcinogenic metabolites during DMBA induced hamster buccal pouch carcinogenesis. PMID: 22806881
    No preview · Article · Jul 2012 · Pathology & Oncology Research
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    ABSTRACT: Cell surface glycoconjugates play an important role in pinocytosis, cell differentiation, intra cellular recognition, cell adhesion and tumorigenesis. Aim of the present study was to investigate the protective effect of androgra-pholide on cellular integrity by measuring the status of glycoconjugates in the buccal mucosa and plasma of ham-sters during 7,12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Oral squam-ous cell carcinoma was developed in the buccal pouch of hamsters by painting with DMBA three times a week for 14 weeks. The status of glycoconjugates was assayed in plasma and buccal mucosa using the specific calorimetric methods. We noticed 100% tumours formation with marked abnormalities in the status of plasma and buccal mu-cosa glycoconjugates in hamsters treated with DMBA alone. Oral administration of andrographolide at a dose of 50 mg/kg bw not only completely prevents the tumour formation, but also restored the status of glycoconjugates in DMBA treated hamsters. The present study thus demonstrates the protective effect of andrographolide on cell surface glycoconjugates abnormalities during DMBA-induced hamster buccal pouch carcinogenesis.
    Full-text · Article · Jan 2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: The present study has investigated the modulating effect of carnosic acid on the expression pattern of cell proliferative (proliferating cell nuclear antigen (PCNA) cyclin D1 and a transcription factor c-fos), apoptotic (p53, Bcl-2, Bax caspase -3 and 9), inflammatory (Nuclear factor kappa B (NFκB) and cyclooxygenase-2 (COX- 2) and angiogenic (vascular endothelial growth factor (VEGF) markers during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. Oral tumors were developed in the hamsters buccal pouches by painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. Hundred per cent tumour formation (well-differentiated squamous cell carcinoma) accompanied by deregulation in the above mentioned molecular markers was noticed in hamsters treated with DMBA alone (tumour bearing hamsters). Oral administration of carnosic acid at dose of 10mg/kg bw to hamsters treated with DMBA not only completely prevented the tumour formation, but also corrected the abnormalities in the expression pattern of molecular markers. The present study suggests that carnosic acid might have inhibited the tumour formation by exerting anti-cell-proliferative, anti-inflammatory, anti-angiogenic and apoptotic potential during DMBA-induced hamster buccal pouch carcinogenesis.
    No preview · Article · Jan 2012 · African Journal of Traditional, Complementary and Alternative Medicines
  • Nagarethinam Baskaran · Duraisamy Rajasekaran · Shanmugam Manoharan

    No preview · Article · Jan 2011 · International Journal of Nutrition, Pharmacology, Neurological Diseases