[Show abstract][Hide abstract] ABSTRACT: 2014): Array-based genotyping and genetic dissimilarity analysis of a set of maize inbred lines belonging to different heterotic groups -Genetika, Vol 46, No. 2, 343-352. Here we describe the results of the detailed array-based genotyping obtained by using the Illumina MaizeSNP50 BeadChip of eleven inbred lines belonging to different heterotic groups relevant for maize breeding in Southeast Europe -European Corn Belt. The objectives of this study were to assess the utility of the MaizeSNP50 BeadChip platform by determining its descriptive power and to assess genetic dissimilarity of the inbred lines. The distribution of the SNPs was found not completely uniform among chromosomes, but average call rate was very high (97.9%) and number of polymorphic loci was 33200 out of 50074 SNPs with known mapping position indicating descriptive power of the MaizeSNP50 BeadChip. The dendrogram obtained from UPGMA cluster analysis as well as principal component analysis (PCA) confirmed pedigree information, undoubtedly distinguishing lines according to their background in two population varieties of Reid Yellow Dent and Lancaster Sure Crop. Dissimilarity analysis showed that all of the inbred lines could be distinguished from each other. Whereas cluster analysis did not definitely differentiate Mo17 and Ohio inbred lines, PCA revealed clear genetic differences between them. The studied inbred lines were confirmed to be genetically diverse, representing a large proportion of the genetic variation occurring in two maize heterotic groups.
[Show abstract][Hide abstract] ABSTRACT: The rate of genome damage elimination after therapeutic exposure to ionising radiation was estimated in stage I testicular seminoma patients monitored over a seven-year follow-up. DNA damage elimination in peripheral lymphocytes of ten subjects was analysed by the chromosome aberration assay. Seven years after the end of radiotherapy, signifi cantly increased frequency of ring and dicentric chromosomes was still detected in comparison with baseline values. These results indicate the induction of genome instability. Long-term follow-up studies of cancer patients after radiotherapy could give us valuable information on the rate of genome damage elimination after exposure to ionising radiation and about the duration and manifestation of genome instability. This may be used in health risk assessment related to the possible development of secondary neoplasia. Studies such as this could have a great value both for oncology and radiation protection management protocols, especially after accidental overexposures.
[Show abstract][Hide abstract] ABSTRACT: Digitalization of the HTV News Programme has changed the techniques used so far for archiving the AV material. Access to the server now gives archivists more independency in their work and also a more direct contact with the work of journalists (raw material and semi–finalized products). This has made possible a more creative approach to archiving, which is one of the crucial aspects of the production, required at the end of production, but often at its beginning as well. Digitalization enables the creation of on–line archives – the Workspace within the scope of the Media Manager. The archives are created from the existing analogue material entered into the server and from the material that is already on the server, which – due to its (most often) use value – needs to be part of the Workspace Archives. As a result, the archives are divided in the so – called »legacy« (existing, inherited) and »born« (newly established) archives. The first ones contain materials from the old media and their access to the digital world is possible only by migration. The other archived material is available on the server upon direct ingestion, or transfer from the digital storage. Its content may never be copied and physically handled. Instead, it will remain stored for the virtual availability to users.
[Show abstract][Hide abstract] ABSTRACT: This study attempts to answer the question if any level of head and neck circulation takes a part in development of Age-Related Macular Degeneration (ARMD) and hearing and equilibrium impairments. Condition of large blood vessels was examined by Color-Doppler ultrasound, and carotid and ophthalmic arteries were included. The microcirculatory changes were examined directly by fundus photography and fluorescein angiography and indirectly testing hearing and equilibrium. The study group included 40 patients (21 females, 19 males) aging from 53 to 84 years with different stages of ARMD. The control group included 40 patients (18 females, 22 males) aging from 51 to 82 years without ARMD. Patients were inhabitants of Primorsko-Goranska County. There was no relationship between ARMD and condition of large blood vessels because significant stenosis of carotid arteries was found in 2 patients (5%) in study group and 3 patients (7.5%) in the control group (p>0.05). On the contrary, we found correlation between ARMD and hearing (p=0.0127) and equilibriium impairments (p=0.0242). Fluorescein angiograms shows raised number of ischemic retinal capillaries in patients with ARMD (p=0.0053). Results lead to conclusion that circulatory disorders on microcirculatory level take a great part in development of ARMD and hearing and equilibrium impairments in the elderly. The key is damage of sensory cells of the retina and inner ear caused by microcirculatory disorders. Interesting data was noticed that 9 patients with more serious ARMD on one side of head had greater hearing loss on the same side. If we find a new treatment for microcirculatory disorders, maybe we can treat both sensory impairments in earlier stage.
Full-text · Article · Apr 2010 · Collegium antropologicum
[Show abstract][Hide abstract] ABSTRACT: The aim of this study is to evaluate the content of phenolics: total phenols (TPC), flavonoids (TF), anthocyanins (TA), flavan-3-ols (TF3ols), as well as total antioxidant capacity (TAC) and reducing power (RP) in four blueberry cultivars (Vaccinium corymbosum L.) introduced in the Northwest Croatian climate conditions. Phenolic compounds were measured by spectrophotometric methods, TAC was determined using DPPH and ABTS assays and RP by FRAP assay. All cultivars contained high mass fraction of TPC, TF, TA and lower mass fraction of TF3ols. Among the researched fruits, Sierra cultivar contained the highest amounts of all groups of phenolics, followed by Elliott>Bluecrop≥Duke. Significant differences were observed in phenolic mass fraction among different cultivars and growing seasons (p<0.05), and phenolic compounds were significantly higher in growing season 2006. Examined cultivars possess high antioxidant capacity and reducing power, and all phenolics were highly correlated with TAC and RP (R=0.46 to 0.99). The study indicated that growing and climate conditions in Northwest Croatia are convenient for introducing blueberry cultivars. Generally, blueberry fruits are a rich source of phenolics, which show evident antioxidant capacity.
Full-text · Article · Jan 2010 · Food Technology and Biotechnology
[Show abstract][Hide abstract] ABSTRACT: Aim: The aim is to determine possible advantage of application of Tramadolum, Diclofenacum and Dexamethasonum in therapy of postoperative pain after lumbar discectomy in relation to standard postoperative application of Tramadolum and Metamizolum Natricum. Methods: The research covered 46 patients with mean age of 45.29 ± 14.06. All patients underwent open lumbar discectomy. The first group, consisting of 23 patients, was treated with Tramadolum and after the surgery , and the other one, also with 23 patients, was treated with Tramadolum, Dexamethasonum and Diclofenacum. The VAS questionnaire of postoperative pain consisted of two parts: the first one, filled in by a surgeon, included clinical findings, and the second one, filled in by a patient, contained data entered by the patient on postoperative pains and painful discomforts two and eight days after the surgery, with the scale from 0 to 10. Statistical 5.0 programme was used in statistical analysis. Statistical significance of differences was determined by Student’s independent samples T-test, while significance of differences between individual samples in respect of pain and time of filling in the questionnaire was determined by Student’s dependent samples T-test. Results: The research results, not considering measurement of preoperative pain, show that the group treated with Tramadolum, Dexamethasonum and Diclofenacum experienced significantly lower pain in the leg area (p< 0.01), while pain in sacral area was somewhat lower but still with statistical significance (p = 0.03; p = 0.01) compared to the group receiving Tramadolum and Metamizolum Natricum. Conclusion: The research results confirm efficiency and advantage of combined therapy with Tramadolum, Diclofenacum and Dexamethasonum in coping with postoperative pain after lumbar discectomy compared to the therapy with Tramadolum and Metamizolum Natricum.
[Show abstract][Hide abstract] ABSTRACT: The opportunistic cervical cancer screening has been conducted in Croatia since its introduction in the 1960s, in the context of a high quality gynaecological cytology with a long tradition and a wide network of primary care gynaecologists. In 2006, a pilot screening programme under the title »Early detection of cervical cancer was conducted in Primorsko-Goranska County (PGC)«, as the first organised cervical cancer screening ever conducted in the Republic of Croatia. The pilot screening programme targeted women aged 20–64 years. The pilot group consisted of 6,000 randomly sampled primary care patients of six gynaecologists. The women were invited via a personal letter and were given a questionnaire. The results of the first and the second year of screening, as well as of both years together were analysed. The response rate to the anamnestic questionnaire was 49.1%. The participation rates to the screening were 35.2% in 2007, and 46.5% in 2008, total of 42.7%. The increase in participation between years 2007 and 2008 was statistically significant (p=0.01). According to the age, the lowest participation rate of 33.3% was observed in the youngest group of women (20–29) and the highest of 60.7% in the oldest group (60–64). The detection rate of cytological abnormalities was 4.6% with 2.6% of borderline (ASCUS) cytology and referral rate of 1.2%. The highest abnormal Pap test frequencies of 6.8% and 7.1% were observed in the youngest age groups (20–29 and 30–39), and the lowest (2%) in the age group of 60–64. Specimen adequacy was generally of high quality with unsatisfactory rate of 0.8%, with statistically significant improvement in 2008, compared to the previous year (p=0.001). Although to a limited extension, during two-year pilot cervical cancer screening programme in PGC the participation rates and Pap smear adequacy have improved. We expect that the continuation of the programme will result in further increase of participation and higher overall quality of the programme.
[Show abstract][Hide abstract] ABSTRACT: Carotid artery stenosis (CAS) is one of the major complications of external irradiation (radiotherapy, RT) for laryngeal carcinoma. Considering amaurosis fugax is often one of the first signs of significant CAS our focus was to determine weather patients with post-irradiation CAS also develop ocular microangiopathy as a result of insufficient ophthalmic circulation. In our study Carotid Duplex ultrasound scans revealed that 33.33% of patients had significant radiation–induced CAS. The majority (over 85.71%) of radiation-induced CAS had more than one atherosclerotic plaque including any degree of stenosis in the RT group, and had significantly more than that of the control group. Microangiopathic changes were documented only with the patients that have had an increased cerebrovascular risk (diabetes mellitus and arterial hypertension).
[Show abstract][Hide abstract] ABSTRACT: The purpose of the present study was to investigate the bilateral deficit (BLD) in elite sprinters and examine the relationship between the BLD and sprint start performance. Twelve male elite sprinters (age: 22.41±3.39 years, 100m personal best: 10.82±.25s) performed sprint starts, two- and one-leg counter- -movement jumps (CMJ). A system of eight CCD cameras with a frequency of 200 Hz was used for the 3D kinematic measurements of CMJ. The ground reaction forces of sprint starts and vertical jumps were measured unilaterally and bilaterally by means of two independent and synchronized force platforms. Significantly lower values of force production of the front leg in the double start compared to the force production in the single start indicated the existence of a phenomenon similar to the bilateral deficit (BLD). The main findings of the present study were that: 1) lower values of BLD in the CMJ are related to higher peak force production of the rear leg in the double start of the sprint start (r=-.630; p=.000), 2) lower BLD in the CMJ is also related to higher total impulse of force on the blocks (r=-.550; p=.000) and 3) BLD values in CMJ are higher in elite sprinters compared to team sport athletes examined in the previous studies. The BLD measured in CMJ is a good indicator of a lower performance in the sprint start. As a consequence, the sprinters with higher BLD produced a lower total impulse of force on the blocks and lower block velocity, which are related to the overall 60m and 100m sprint performance.
[Show abstract][Hide abstract] ABSTRACT: Lichen planus is a chronic immunologic inflammatory disease that frequently involves oral mucosa. Systemic diseases and medications are considered to be precipitating factors in the development of oral lichen planus. Objective: The purpose of this study was to examine the prevalence of systemic diseases and medications in patients with oral lichen planus (OLP) and to determine possible causal relationship. Materials and methods: Retrospective data included medical records of 145 patients, 104 women and 41 men who were referred to the Department of Oral Medicine. All patients had clinical and histopathological criteria fulfilled for diagnosis of oral lichen planus. Data were collected for the period of two years (autumn 2007- autumn 2009). These data were compared with the prevalence of systemic diseases in patients with oral leukoplakia (LPL) and general Croatian population according to the national statistical register. The same comparison was made for the used medications. Obtained data were analyzed by use of χ2 test with level of significance p<0,05. Results: Systemic diseases associated with OLP were found in 77.9% of patients. The most frequent disease was hypertension. Frequency of systemic diseases between patients with OLP and LPL has shown statistically significant difference. Conclusion: Increased prevalence of hypertension among OLP patients in comparison to the prevalence in the general population can not be interpreted as the etiological factor for the development of OLP since hypertension is the most prevalent disease in general population.
[Show abstract][Hide abstract] ABSTRACT: Based on the known approaches and published mathematical models, as well as on theoretical consideration using our experimental data, the integrated approach to mathematical modelling of atrazine biodegradation processes has been employed and sophisticated mathematical models for different reaction systems have been developed. The applicability of these models which take into account physical, chemical, biochemical and biological complexity of atrazine biodegradation was further analyzed in comparison with mathematical models describing simple consecutive reaction systems using first-order kinetics. Kinetics of atrazine degradation in liquid media and soil contaminated with atrazine at the temperatures of 10 and 30 °C was assessed and compared. Biodegradation experiments in liquid media were conducted at atrazine concentrations ranging from 0.14 to 25 mmol/L, while the experiments in soil were conducted at atrazine concentration of approx. 0.44 μmol/g. Computer simulations were applied to explain experimental results and test the adequacy of mathematical models. Detailed analysis of computer simulation data showed that the developed integrated mathematical models could be considered as the most convenient for describing kinetics of atrazine biotransformation in both liquid media and contaminated soil, although even simple mathematical models are suitable for explaining some experimental results, especially when evaluating the temperature effects on biodegradation efficacy of the applied mixed bacterial culture.
[Show abstract][Hide abstract] ABSTRACT: Strong competition and continuously changing market demands require constant change and improvements of a company’s business. For production companies, it is very important to keep their production processes efficient by constantly improving them. In this paper the production management process approach is presented, as well as the fundamentals of Lean production. Value stream mapping has proved as one of the efficient tools for the analysis of production. Application of this tool for the analysis of waste in production process is presented in the case study.
[Show abstract][Hide abstract] ABSTRACT: The purpose of this study was to determine microbial population of “Slavonian kulen” produced in the traditional way, in rural house- holds, because it is a risk group of food which can be a source of various causes of disease. Furthermore, as a part of the indigenous microbial population and potential starter cultures that are responsible for a certain avor and texture of traditional meat products, except for lactic acid bacteria, bacteria of the genus Staphylococcus were isolated. In analyzed samples of “Slavonian kulen”, neither sample showed evidence of pathogen bacteria. Among isolates of the genus Staphylococcus, a typical representative of starter cul- tures for meat, there prevailed: S. xylosus (5.56 log10CFU/g), S warneri (5.36 log10CFU/g), S. lentus (3.30 log10CFU/g) and S. auricula- ris (3.30 log10CFU/g). All staphylococcal isolates, except for the isolates of S. auricularis SK4, showed signicant antimicrobial activity to the test pathogenic microorganisms through the production of high concentrations of lactic acid, and showed one of the impor- tant properties of potential starter cultures. Furthermore, the isolate S. xylosus SK1 has also shown adhesion properties on epithelial cells of mice intestine and thus satised one of the selection criteria for the selection of probiotic strains. The strongest inhibitory eect on the growth of pathogenic isolates was showed by S. xylosus SK1 and S. warneri SK2, whereas S. auricularis SK4 follows the growth curve of the selected test organisms.
[Show abstract][Hide abstract] ABSTRACT: Wood processing and furniture manufacture in the Republic of Croatia have been developed on high quality forest raw material, and their activities are based on the use of forest raw material, long wood-processing tradition and good quality of human resources. Therefore, this is an important economic segment of the country. However, due to global economic trends, and also due to inadequately developed and low production efficiency, insuffi cient use of capacities and underdeveloped production of higher processing stages, the total income of the industrial wood processing is relatively low with respect to the potential of raw materials. It is, therefore, important to determine the position of the sector with the focus on market research. For this reason, the analysis was conducted and comparison made between certain market indicators such as production, exports, imports, consumption, and sales share of Croatian manufacturers, wood processing (DD 20) and furniture manufacture (DN 36), in the domestic market. In the observed period, an increasing trend was recorded of the production of other wood products and furniture manufacture, in favor of production of other wood products. From 2000 to the end of 2008 export values of other wood products were higher in relation to furniture export values, while in the field of import determinant values reversed. In the observed period, product sales of Croatian manufacturers in sectors DD 20 and DN 36 in the domestic market were variable.
[Show abstract][Hide abstract] ABSTRACT: Cold haemagglutinin disease (CHD) was diagnosed in two newborn French bulldog pups. Necropsy revealed cyanosis and necrosis, formation of crusts on all anatomic extremities and amputation of the lower parts of the hind limbs. Histologically, cutaneous necrosis was found on the limbs of both animals, with blood vessel thrombosis, hemorrhages and predominantly neutrophilic infiltration, which affected the periosteum of the metatarsal bones. The surfaces of the necrotic areas were covered with thick crusts with Gram positive bacterial colonies. In the spleen and liver erythrophagocytosis was present. Lung congestion, alveolar edema and thrombosis of pulmonary artery branches, cerebral and chorioidal vessels were present. Immunohistochemicaly, lymphocytes and plasma cells in necrotic tissue were positive for IgM. Bacteriological examination of swabs from necrotic areas revealed the presence of Staphylococcus aureus. The diagnosis of CHD was based on macroscopic and histopathologic findings, which were identical to those described in literature and were supported by immunohistochemical reactivity to IgM.
[Show abstract][Hide abstract] ABSTRACT: Tourism as one of the most distinct modern socio-economical phenomena of the world in its quantitative indicators is approaching gradually in the framework of international relations to billions of participants, with the economic effects that exceed the 800 billion USD. These sizes are almost triplicate by inclusion of domestic tourist migration, which gives more impressive social and economic dimension to a whole figure. It is logical that this movement with their economic effects are accomplishing 30% of world trade exchanges, 17% GDP, 12% of employment, 7% of investments, through direct, conversion or multiplier effects includes almost all spheres of modern economic and social superstructure and are manifesting very expressive with their positive but also possible negative connotations to the sociological and especially on the spatial sphere. Today very actual and extended mutual interactions between tourism, health and health culture in the modern society in this topic shall be analyzed considering two basic aspects and they are the following: - Influence of level of health culture in the area of tourist initiative about making decision on joining the tourist migration as the important element of health preservation, and the quality of living, being familiar with all medical advantages in temporary changing working and living environment.. - Influence of health tourism as polyfunctional social phenomenon on the health of tourists and the level of health and social culture of certain area where it is developed in the context of accompanying possible negative consequences of intensive tourist development on the environment space and social sphere.
[Show abstract][Hide abstract] ABSTRACT: Mycelial growth and afl atoxin B1 accumulation by toxigenous mould Aspergillus flavus were inhibited by dehydroacetic acid and its newly synthesized analogues, 4-hydroxy-3-(p-toluoyl)-6-(p-tolil)-2H-yrane-2-one (DHT) and 5-Bromo-4-hydroxy-3-(p-toluoyl)-6-(p-tolil)-2Hpyrane-2-one (BrDHT) during the growth of investigated mould on solid substrate (soy-beans). Experiments were carried out in a stationary culture at 20 0C and 30 0C, respectively, during 21 days. Mycelial growth (biomass dry weight) was determined by measuring the chitin content and concentration of afl atoxin B1 (AFB1) was quantitated by HPLC. Concentrations of DHA and DHT of 0.1 and 0.2 μg x g-1 of substrate were stimulated, but concentrations higher than 0.5 μg x g-1 of these compounds, mould growth and AFB1 accumulation partially inhibited. In experiments with 0.2 μg x g-1 of BrDHT mould growth and AFB1 accumulation were inhibited completely depending on the time and temperature of cultivation.
[Show abstract][Hide abstract] ABSTRACT: Aim: Menopausal women, due to lack of hormones-estrogen are exposed to accelerated bone loss leading to bone defi ciency diseases, such as osteoporosis. In menopause, other risk factors such as: low body mass index, unbalanced diet and physical inactivity may reduce bone mass. The aim of this study was to examine whether, and to what extent anthropometric parameters and age predict the value of quanti tati ve ultrasound parameters of the calcaneus in women with respect to hormonal status. The study included 499 women of the City of Rijeka. Methods: Peripheral density of the calcaneus was measured using an ultrasound machine Clinical Bone Sonometar Sahara Hologic (Hologic Inc., Massachusett s, USA). The measurements were performed in sitting subjects, at a constant location (right foot). Results: Stati sti cal analysis of all subjects has determined that independent variables (age, menopause, anthropometric parameters) signifi cantly predict the value of quanti tati ve ultrasound parameters of the calcaneus. In women who are more than 5 years of menopause, the same independent variables, with the excepti on of menopause significantly predicted the value of quanti tati ve ultrasound parameters of the calcaneus. Conclusion: In menopause, the beneficial effect of hormones on the physiological processes in bone ti ssue is absent, which therefore become catabolic. In such conditi ons, achieved body mass remain the only backup system that preserves skeletal system from bone deficiency diseases such as osteoporosis and osteopenia.