Bing Li

Changchun Normal University, Hsin-ching, Jilin Sheng, China

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Publications (66)136.79 Total impact

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    ABSTRACT: We have performed in situ low-temperature Raman spectroscopy studies on iodine molecules confined in the one-dimensional (1D) round channels of AlPO4-5 (AFI) crystals. Polarized Raman measurements show that the Raman intensity of individual iodine molecules parallel to channel axis (termed lying molecules) increases more than that of iodine molecules perpendicular to channel axis (termed standing molecules) upon cooling temperature down to -196 °C. This suggests that an orientation transformation of individual iodine molecules has occurred to increase the population of iodine molecules lying along channel axis as temperature decreases. Consequently, the corresponding Raman mode shows a redshift due to the enhancement of intermolecular interaction. This low-temperature induced orientation transformation is observed to recover when heated back to room temperature, indicating that orientation of iodine molecules confined in this porous medium can be modulated reversibly at a molecular level by cooling temperature down.
    No preview · Article · Feb 2016 · Microporous and Mesoporous Materials

  • No preview · Article · Feb 2016
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    ABSTRACT: Introduction: Reverse transcriptase polymerase chain reaction assay (RT-PCR) has been proved to have high sensitivity and specificity to detect anaplastic lymphoma kinase (ALK) rearrangements. The aim of this study was to investigate the response to crizotinib in patients of advanced non-small-cell lung cancer (NSCLC) with ALK rearrangements detected by RT-PCR. Methods: Only patients with advanced NSCLC who had their ALK rearrangement status detected by RT-PCR were included in this analysis. The utility of RT-PCR and fluorescence in situ hybridization assay (FISH) were compared in patients who were treated with crizotinib based on their positive ALK rearrangements. Results: 1010 patients were included in this study. Among them, 104 patients were ALK RT-PCR positive and 53 of them received crizotinib treatment. Among 255 tumors simultaneously analyzed by FISH and RT-PCR, the latter successfully detected all the 25 tumors with arrangements, including two cases that were missed by FISH. The overall response rate (ORR) and median progression free survival (mPFS) of the 53 patients with ALK rearrangements who received crizotinib treatment were 60.4% (95%CI, 47.2-73.6) and 8.4 months (95% CI 6.75-10.05) respectively, which were similar to the 21 patients detected by FISH with ORR of 57.1% (95% CI 33.3-76.2) (p=0.799) and mPFS of 7.4 months (95% CI 4.43-10.38) (p=0.833) after crizotinib treatment. Interestingly, there were 2 patients responded to crizotinib had their ALK rearrangement detected by RT-PCR but not FISH. Conclusions: RT-PCR should be considered as an alternative/supplemental approach to detect ALK fusion oncogene in NSCLC patients who might benefit from crizotinib treatment.
    No preview · Article · Sep 2015 · Journal of thoracic oncology: official publication of the International Association for the Study of Lung Cancer
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    ABSTRACT: Raman spectra of iodine species confined in one-dimensional elliptical channels of AlPO4-11 (AEL) crystals have been studied from room temperature down to −196 °C. As temperature decreases, thermal fluctuations of individual iodine molecules confined in AEL channels are slowed down and they prefer to rotate to channel axis direction, which increases the population of iodine molecules along channel axis (i.e., lying molecules and chains). Such temperature-driven orientation transformation of iodine molecules is found to be reversible upon heating up to room temperature. The experimental observations are in good agreement with our theoretical simulations by molecular dynamics on low density iodine-filled AEL crystals. We thus provide a new way to modulate the orientation of iodine molecules in nanochannels, which may have implications in low-temperature-sensitive nanoscale devices. Copyright © 2015 John Wiley & Sons, Ltd.
    No preview · Article · Mar 2015 · Journal of Raman Spectroscopy
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    ABSTRACT: Nb doped TiO2 microspheres modified with WO3 (Nb-TiO2/WO3) was prepared by ultrasonic spray pyrolysis method combined with impregnation method. The microspheres were characterized with SEM, TEM, XRD, BET, photoluminescence and UV-vis absorption spectra. The Nb-doping was observed to extend the spectral absorption of TiO2 into visible spectrum, and the absorption onset was red-shifted for about 88 nm compared to pristine TiO2 microspheres. Nb-TiO2/WO3 microspheres do not display a red-shifted absorption edge compared with Nb doped TiO2 microspheres. Under solar irradiation, Nb-TiO2/WO3 microspheres showed higher photocatalytic activity for methylene blue degradation compared with that of pure TiO2 microspheres and Nb doped TiO2 microspheres, which could be ascribed to the extended light absorption range and the suppression of electron-hole pair recombination.
    No preview · Article · Mar 2015 · Materials Research Bulletin
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    ABSTRACT: Epithelial to mesenchymal transition (EMT) has profound impacts on cancer progression and also on drug resistance, including epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs). Nowadays there is still no predictive biomarker identified for the use of EGFR-TKIs in non-small cell lung cancer (NSCLC) patients with wild-type EGFR. To clarify the role of EMT phenotype as a predictive marker for EGFR-TKI, we performed a retrospective study in 202 stage IV or recurrent NSCLC patients receiving gefitinib or erlotinib therapy from Jun 2008 to Sep 2012 in our institute. Clinical data and EGFR mutational status were collected, while epithelial, epithelial to mesenchymal, not specified or mesenchymal phenotype were classified according to EMT markers such as E-cadherin, fibronectin, N-cadherin and vimentin by immunohistochemistry. Epithelial phenotype was more frequently found in patients with EGFR mutation (P=0.044). Epithelial phenotype was associated with a significantly higher objective response rate (23.5% vs 11.1% vs 0.0% vs 2.4% p=0.011), longer PFS (4.4 vs 1.9 vs 1.7 vs 1.0 months, P<0.001) and longer OS (11.5 vs 8.9 vs 4.5 vs 4.9 months, P<0.001) compared to epithelial to mesenchymal, not specified and mesenchymal phenotype in the wild-type EGFR subgroup. In the subgroup with EGFR mutation, the trend remained but without a statistically significant difference. In conclusion, epithelial phenotype was more likely expressed in patients with EGFR mutation and was associated with a better outcome in advanced NSCLC patients with wild-type EGFR, which indicates that the EMT phenotype might be a potential marker to guide EGFR-TKI therapy in this population. © 2014 Wiley Periodicals, Inc.
    No preview · Article · Dec 2014 · International Journal of Cancer
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    ABSTRACT: To investigate the effectiveness of double arthrodesis to correct flatfoot deformity with pes valgus. Between May 2009 and May 2012, 12 patients with flatfoot deformity and pes valgus were treated using subtalar and talonavicular joints arthrodesis through a single medial incision approach. There were 5 males and 7 females with an average age of 53.3 years (range, 21-78 years), including 5 left feet and 7 right feet. Of them, 11 cases had posterior tibial tendon dysfunctions; 6 cases were at Johnson-Strom stage III, 5 cases at stage II(c); and 1 case had tarsal coalition. Preoperative American Orthopaedic Foot and Ankle Society (AOFAS) score and visual analogue scale (VAS) score were 48.75 ± 3.46 and 6.08 ± 1.14, respectively. The mean operation time was 85.6 minutes (range, 65-125 minutes). Eleven patients were followed up for 19.4 months on average (range, 13-30 months). All of the cases obtained primary healing of incision, with no complication of infection and nerve or blood vessel injury. X-ray film showed that the mean time of bone union was 9.8 weeks (range, 7-18 weeks): no bone nonunion occurred. No loosening or breakage of internal fixation was observed. Pain occurred at the calcaneal-cuboid joint (1 case) and at fixation site (1 case), and was relieved after symptomatic treatment. The mean AOFAS score and VAS score were significantly increased to 81.36 ± 2.98 and 0.72 ± 0.11 respectively, showing significant differences when compared with preoperative scores (t = 19.946, P = 0.000; t = 16.288, P = 0.000). Subtalar and talonavicular joints arthrodesis by a single medial incision approach is a useful alternative to triple arthrodesis for the correction of flatfoot deformity with pes valgus.
    No preview · Article · Nov 2014 · Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery

  • No preview · Article · Oct 2014 · Foot & Ankle International
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    Huihui You · Shaohui Chen · Lin Mao · Bing Li · Ye Yuan · Rui Li · Xu Yang
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    ABSTRACT: Di-(2-ethylhexyl) phthalate, as the most commonly used plasticizer, is considered to be related to the asthma prevalence. There are studies affirming that the DEHP has an adjuvant effect in the pathogenesis of allergy asthma. Oxidative stress is one possible pathway for DEHP-adjuvant effect. Thus, this study explored whether DEHP could induce adjuvant effect in mouse asthma model via oxidative stress pathway. Male BALB/c mice were randomly divided into six groups: (1) saline group, (2) DEHP group, (3) ovalbumin (OVA) group, (4) DEHP+OVA group, (5) OVA +vitamin E (Vit E) group, (6) DEHP+OVA+Vit E group. The exposure dose of DEHP was 30 mg/kg body weight (bw)/day. After 18 days of the exposure protocol. Reactive oxygen species (ROS), glutathione (GSH) and malonaldehyde (MDA) levels and biomarkers related to asthma model were measured. Collectively, these data indicated higher ROS and MDA levels and lower GSH contents in DEHP+OVA group than that in OVA group, while Vit E, an antioxidant, could restore ROS, MDA and GSH levels to control levels and attenuate the DEHP and/or OVA effects. Our observations suggested that there was a relationship between oxidative stress and the adjuvant effect induced by DEHP in this mouse asthma model.
    Preview · Article · Jun 2014 · Food and Chemical Toxicology

  • No preview · Article · May 2014
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    ABSTRACT: Posterior pilon fractures are rare injuries and have not yet gained well recognition. The purpose of this study was to present the treatment outcome for patients with posterior pilon fractures treated with buttress plate. In this retrospective study we identified patients with posterior pilon fractures of the distal tibia who had undergone open reduction and internal fixation at our institute. Between January 2007 and December 2009, 10 patients (mean age, 46.5 years) who had undergone buttress plating via either a posterolateral approach or a dual posterolateral-posteromedial approach, were selected. All 10 patients were available for follow-up. The clinical outcome was evaluated with the American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot score and the visual analogue scale (VAS). The radiological evaluation was performed using the osteoarthritis-score (OA-score). Satisfactory reduction and stable fixation were accomplished in all patients. At a mean follow-up of 36.2 months, all patients had good radiological results and showed satisfactory clinical recovery. The mean AOFAS sore was 87.8, the mean OA-score was 0.6, and the mean VAS scores during rest, active motion, and weight-bearing walking were 0.6, 0.8, and 1.4, respectively. Buttress plating for posterior pilon fractures gave satisfactory clinical outcomes. It also ensured rigid fixation which in turn enabled earlier postoperative mobilization. Level of Evidence IV, Retrospective Study.
    Preview · Article · Mar 2014 · Acta Ortopédica Brasileira
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    ABSTRACT: TiO2-graphene porous microspheres were prepared by ultrasonic spray pyrolysis (USP) of aqueous suspension of graphene oxide containing TiO2 nanoparticles (Degussa P25). The composite microspheres were characterized with SEM, XPS, photoluminescence, Raman and UV-Vis absorption spectra. TiO2-graphene porous microspheres displayed higher photocatalytic activity for the degradation of methylene blue solution than pristine TiO2 microspheres under the irradiation of Xe lamp, and the highest activity was obtained at a weight percentage of graphene around 1%. The effect of graphene on photocatalytic activity of porous microsphere was discussed in terms of the enhanced charge separation by TiO2-graphene heterojunction, increased absorption of the visible light, as well as the possible hindrance of mass transportation in microspheres.
    No preview · Article · Jan 2014 · Journal of Alloys and Compounds
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    ABSTRACT: To explore the anatomy of the plantar aponeurosis (PA) and its biomechanical effects on the first metatarsophalangeal (MTP) joint and foot arch. Anatomic parameters (length, width and thickness of each central PA bundle and the main body of the central part) were measured in 8 cadaveric specimens. The ratios of the length and width of each bundle to the length and width of the central part were used to describe these bundles. Six cadaveric specimens were used to measure the range of motion of the first MTP joint before and after releasing the first bundle of the PA. Another 6 specimens were used to evaluate simulated static weight-bearing. Changes in foot arch height and plantar pressure were measured before and after dividing the first bundle. The average width and thickness of the origin of the central part at the calcaneal tubercle were 15.45 mm and 2.79 mm respectively. The ratio of the length of each bundle to the length of the central part was (from medial to lateral) 0.29, 0.30, 0.28, 0.25, and 0.27, respectively. Similarly, the ratio of the widths was 0.26, 0.25, 0.23, 0.19 and 0.17. The thickness of each bundle at the bifurcation of the PA into bundles was (from medial to lateral) 1.26 mm, 1.04 mm, 0.91 mm, 0.84 mm and 0.72 mm. The average dorsiflexion of the first MTP joint increased 10.16° after the first bundle was divided. Marked acute changes in the foot arch height and the plantar pressure were not observed after division. The first PA bundle was not the longest, widest, or the thickest bundle. Releasing the first bundle increased the range of motion of the first MTP joint, but did not acutely change foot arch height or plantar pressure during static load testing.
    Full-text · Article · Jan 2014 · PLoS ONE
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    Huihui You · Shaohui Chen · Lin Mao · Bing Li · Ye Yuan · Rui Li · Xu Yang
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    Preview · Article · Jan 2014
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    ABSTRACT: Waveband-dependent photochemical reduction properties of graphene oxide (GO) were investigated. The ratio of oxygenated carbon to graphitic carbon of the GO film was reduced to 39% and 28% by 400-800 nm and 350-800 nm irradiation, respectively from the initial value of 47%, while this ratio changed negligibly by 520-800 nm irradiation. This waveband-dependent photoreduction is closely related to the wide size distribution of sp2 carbon clusters with different band gaps within the GO sheet, which play the role of the photochemical reacting centers. In addition, the photochemical activity of GO was also demonstrated by photoreduction of Ag+ in water. Our research suggests the possibilities of tuning the microstructure of GO by careful selection of irradiation conditions and of preparing noble metal nanoparticles with a neat surface on GO sheets.
    Full-text · Article · Jan 2014 · RSC Advances

  • No preview · Article · Dec 2013 · International Orthopaedics

  • No preview · Article · Dec 2013
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    ABSTRACT: To explore the operative methods and the short-term effectiveness to repair chronic tears of the 2nd plantar plate. Between June 2012 and June 2013, 14 patients with chronic tears of the 2nd plantar plate were treated. There were 4 males and 10 females with an average age of 65.9 years (range, 51-82 years) and with an average disease duration of 6.2 years (range, 5-9 years). The left side was involved in 5 cases and the right side in 9 cases. One patient had simple hammer toe; 2 patients had hallux valgus and claw toe; and 11 patients had hallux valgus and hammer toes. All of them had unstable the 2nd metatarsophalangeal joints. The results of modified Lachman test and drawer test were positive. The surgical treatment included a Weil osteotomy and plantar plate repair operation for rupture via dorsal incision after correcting hallux valgus. Primary healing of incision was obtained in all patients. Twelve patients were followed up 6-12 months (mean, 8.2 months). Hallux valgus, claw toe, and hammer toe were all corrected. The results of modified Lachman test and drawer test were negative at 3 months after operation. Mild pain of the 2nd metatarsophalangeal joint occurred, but no stiff was observed in 2 cases at 6 months after operation. The midfoot and forefoot score of American Orthopedic Foot and Ankle Society (AOFAS) was 90.50 +/- 3.73 at last follow-up, which was significantly higher (t=21.724, P=0.000) than preoperative score (57.33 +/- 4.99). The plantar plate is the key to maintain the stability of the metatarsophalangeal joints, and surgical repair can achieve good short-term effectiveness in treating chronic tears of the 2nd plantar plate.
    No preview · Article · Dec 2013 · Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
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    ABSTRACT: Background and Purpose With the increasing use of epidermal growth factor receptor tyrosine kinase inhibitor (EGFR TKI) in patients with advanced non-small cell lung cancer (NSCLC), its acquired resistance has become a major clinical problem. Recent studies revealed that miR-21 was involved into the resistance of cytotoxic agents. The aim of this study was to investigate its role in the acquired resistance of NSCLC to EGFR-TKI. Methods EGFR-TKI-sensitive human lung adenocarcinoma cell line PC9 and the acquired resistant cell line, PC9R, were used. Lentiviral vectors were used to infect PC9 or PC9R to regulate the miR-21 expression. The expression of targeted proteins PTEN and PDCD4 was controlled by RNA interference. MicroRNA array, RT-PCR and TaqMan MicroRNA Assays were used to detect miR-21 expression. The MTT and Annexin V assays were used to determine proliferation and apoptosis. Western Blot and immunohistochemistry were used to analyze target protein expression (PTEN, PDCD4, Akt, p-Akt). We also constructed PC9R xenograft tumor model to observe the relationship between miR-21 and EGFR-TKI resistance in vivo and validated it in the clinical serum specimens of NSCLC patients treated with EGFR-TKI. Result MiR-21 was overexpressed in the EGFR-TKI resistant cell line PC9R relative to PC9. The level of miR-21 was reversely correlated with the expression of PTEN and PDCD4 and positive correlated with PI3 K/Akt pathway. Inhibiting miR-21 with lentivirus vector induces apoptosis in PC9R cell line and inhibiting miR-21with ASO suppressed tumor growth in nude mice treated with EGFR-TKI. Furthermore, serum miR-21 expression in NSCLC patients treated with EGFR-TKI was significantly higher at the time of acquiring resistance than at baseline (p < 0.01). Conclusion miR-21 is involved in acquired resistance of EGFR-TKI in NSCLC, which is mediated by down-regulating PTEN and PDCD4 and activating PI3 K/Akt pathway.
    No preview · Article · Nov 2013 · Lung cancer (Amsterdam, Netherlands)

  • No preview · Article · Nov 2013 · Foot & Ankle International

Publication Stats

521 Citations
136.79 Total Impact Points


  • 2015
    • Changchun Normal University
      Hsin-ching, Jilin Sheng, China
  • 2011-2015
    • Tongji University
      • Medical School
      Shanghai, Shanghai Shi, China
  • 2009-2014
    • Northeast Normal University
      • Center for Advanced Optoelectronic Functional Materials Research
      Hsin-ching, Jilin Sheng, China
  • 2008-2014
    • Huazhong (Central China) Normal University
      • College of Life Sciences
      Wu-han-shih, Hubei, China
  • 2012
    • Tongji Medical University
      Shanghai, Shanghai Shi, China
  • 2007-2012
    • Jilin University
      • State Key Lab of Superhard Materials
      Yung-chi, Jilin Sheng, China