B Fernández's scientific contributionswhile working at Instituto de Salud Carlos III (Madrid, Spain) and other institutions

Publications (2)

  • Source
    Abstract: Few cases of allergy to pine nuts have been described. We report a case of anaphylactic reaction to pine nuts. The patient needed to be treated in the emergency room due to a systemic reaction immediately after eating pine nuts. The patient was studied by prick tests and prick by prick tests. Specific IgE was measured by CAP and by SDS-PAGE/immunoblotting by a diffusion method. The patient showed positive prick by prick tests to pine nuts (12 mm of maximum wheal diameter). Specific IgE was... Show More
    Full-text available · Article · Apr 2000 · Allergy
  • F García · I Moneo · B Fernández · J M García-Menaya · Gonzalo
    Abstract: Some members of the Anacardiaceae family, such as cashew nut, pistachio nut and mango, have been reported to cause immediate allergic reactions. We report three cases of anaphylaxis to cashew nuts. With the aim of describing the allergens existing in cashew and pistachio nuts, patients were prick tested with cashew and pistachio extracts. Specific IgE against both nuts was studied by CAP and SDS-PAGE/immunoblotting. It was found that skin tests and specific IgE to cashew and pistachio nuts... Show More
    Article · May 2000 · Journal of investigational allergology & clinical immunology: official organ of the International Association of Asthmology (INTERASMA) and Sociedad Latinoamericana de Alergia e Inmunología

Publications citing this author (66)

    • and genus Nothopegia Blume, have one species in India as well as in Sri Lanka (Ashton et al., 1997;Meijer, 1983). Members of this family in other countries have been investigated for their occurrence (Tien-lu, 1980;Swaminath, 2000;Yi et al.,2004;Wang, 2014), chemical composition and their applications (Garcia et al., 1999;Ippen, 1982;Benzi, 2009;Makom, 2010;Ojewole, 2005;Ameyapoh, 2010;Al Sayed, 2010;Murray, 2009;Descamps, 2011) and as well as for their molecular data (Wang et al., 2014;Miller et al., 2001, Pell, 2004Yi, 2008). However, in Sri Lanka, members of this family have been studied for their ecological contributions (Weerakkody, and Parkinson, 2006;Gunatilleke, et al., 2005;Jayasekara, et al., 2003;Ganashan, 1996;Gunatilleke and Gunatilleke, 1991;De Zoysa, et al., 1988;Jayasekera, 1992) and medicinal values (Kankanamalage, et al., 2014).
    [Show abstract] [Hide abstract] ABSTRACT: Sri Lanka is a tropical island with variety of climatic, geographical and weather combinations. These significant variations have resulted high biodiversity in the country. Sri Lanka together with the Western Ghats is one of the biodiversity hotspots. Presently, Sri Lanka harbors 3,154 species of Angiosperm families of which 894 are endemic. The family Anacardiaceae is one of the flora groups that did not attract attention of the researchers. However, 47.4% of this family, including 46.7% endemics was considered as nationally threatened during the National Red listing in 2012. This study was carried out to investigate the current occurrence and conservation status of the members of this family. Observations on plant species were made in both protected and un-protected areas. This study present the recent occurrences of 14 species in 6 genera with 11 endemics belongs to this family. These species are dispersed in two strict nature reserves, one Man and Biosphere Reserve, one sanctuary, five forest reserves and two un-protected locations. Among the observed species 35.7% including 36.4% endemics are accounted as nationally threatened which includes ‘Critically Endangered’, ‘Endangered’ and ‘Vulnerable’ species. This information will be contributed to establish precise conservation measures and updating of the conservation status of these species in Global and National level red listing.
    Full-text · Article · Jun 2017
    • The literature is mainly based on case reports, and only recently there have been attempts to clarify, at molecular basis, which and how many allergens hold the highest importance in the sensitization (Asero et al., 2014; Cabanillas et al., 2012; Novembre et al., 2012). Several proteins of different molecular weights have been found able to bind IgE (Cabanillas et al., 2012; García-Menaya et al., 2000; Ma et al., 2002; Rodrigues-Alves et al., 2008), but only one allergen from P. koraiensis, a 7S vicilin named Pin ko 1, has been so far properly characterized (Jin et al., 2014 ). Despite the severity of the symptoms , the reported occurrence of allergy to pine nuts seems to be low, and so far these seeds have not yet been included in the European list among the other tree nuts whose presence must be reported on the label, as indicated by the EU Regulation ⇑ Corresponding author.
    [Show abstract] [Hide abstract] ABSTRACT: Pine nuts are a known source of food allergens and several cases of adverse immunological reaction after ingestion have been reported. To protect allergic consumers, methods to unequivocally detect the presence of pine nuts in complex matrices must be developed. A Taqman-based real time PCR method for the detection of Pinus spp. was set up. A homemade pesto spiked at known concentration of pine nut powder was used as model food. Moreover, DNA was purified from commercial foods declaring or not the presence of pine nuts. The method displayed a very high efficiency and specificity for the genus Pinus. The intrinsic LOD was 1 pg of DNA, while the practical LOD evaluated on model foods was 0.1 ppm of pine nuts powder, the lowest ever registered for the detection of food allergens via real-time PCR. Finally, the declared presence/absence of pine nut in commercial foods was confirmed.
    Full-text · Article · Mar 2016