A V Pavlov

Yaroslavl State Medical Academy, Jaroslawl, Jaroslavl, Russia

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Publications (4)0.72 Total impact

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    ABSTRACT: The smears of isolated thyrocytes with micronuclei taken from 25 patients with nodular pathology of the thyroid gland were investigated. In all kinds of benign nodular pathology the number of micronucleated thyrocytes comes to 0.1-0.3%. These values correspond to the spontaneous level of the content of aberrant thyrocytes in the thyroid gland of highly specialized cell populations. In papillary carcinoma of the thyroid gland the average occurrence of micronucleated thyrocytes in the tumor nodule was about 0.4%. In the tissue outside the tumor taken from both the same and the opposite lobes, the content of cells with micronuclei proved to be substantially higher--0.93-1%. So, the marked increase of the concentration of genetically damaged cells in the thyroid parenchyma proved to be characteristic of the carcinoma of the thyroid gland.
    No preview · Article · Feb 2007 · Vestnik khirurgii imeni I. I. Grekova
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    ABSTRACT: We studied the possibility of using the micronucleus test in in vivo experiments on the model of rat follicular thyrocytes prestimulated to cell division (hemithyroidectomy). Single administration of N-nitroso-N-methylurea produced a significant dose-dependent effect on micronucleus formation in thyrocytes and polychromatophilic erythrocytes of the bone marrow. The test system allowed us to reveal a cumulative effect of 2-fold and 4-fold treatment with the mitogen in low or subthreshold doses on the thyroid gland. Our results indicate that the micronucleus test is an informative method for the analysis of the effect of genotoxic agents on the thyroid parenchyma.
    No preview · Article · Feb 2006 · Bulletin of Experimental Biology and Medicine
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    ABSTRACT: This paper presents a detailed description of the method for obtaining of high quality smears of thyrocytes from the fragments of rat (30 animals) and human (20 cases) thyroid gland using the method of enzymatic dissociation in 0.25% aqueous solution of collagenase (collagenase for pancreatic islet isolation type XI, collagen digestive activity 1205 units/mg, Sigma, USA) at 37 degrees C during 1; 1.5; 2; 2.5 and 3 hours. Optimal time for dissociation in each individual case should be determined using control smears. This approach permitted to successively perform morphological, morphometric and cytophotometric analysis of the glandular cells, to evaluate the relative proportions of different cell types (mitotically dividing cells, binucleate cells, aberrant forms with the micronuclei) in animal experiments as well as in the various types of human thyroid pathology.
    No preview · Article · Feb 2006 · Morfologiia (Saint Petersburg, Russia)
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    ABSTRACT: Ploidy of thyroid parenchyma was studied in adult rats in the control and on day 9 after hemithyroidectomy; operated animals received 3 injections of N-nitroso-N-methylurea. The population of follicular thyrocytes is mainly diploid; total count of polyploid cells increased from 4.4% in the control to 8.5% in experimental rats. All thyrocytes containing micronuclei were tetraploid. No diploid micronucleated elements were detected. This suggests that genetically damaged thyrocytes divide by the mechanism of acytokinetic (polyploidizing) mitosis.
    No preview · Article · Jan 2006 · Bulletin of Experimental Biology and Medicine