A Fujiwara

Hiroshima University, Hirosima, Hiroshima, Japan

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Publications (28)11.32 Total impact

  • S Ohta · N Nagai · H Fujimoto · H Kioka · A Fujiwara
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    ABSTRACT: Cervical scrapes from 43 patients (12 with chronic cervicitis, 13 with dysplasia, 11 with carcinoma in situ and 7 with invasive carcinoma) were examined by the polymerase chain reaction (PCR), to detect the amplified E7 gene of the HPV types 16 and 18 DNA sequence. HPV types 16 and/or 18 DNA were detected in 25 of 43 cases by the PCR (type 16 in 16, type 18 in 8, and both types 16 and 18 in 1). In comparison with the results for histological grades, the positive rate significantly increased as the grade of cervical dysplasia became higher. All cases were also examined with a HPV detection kit, the "Vira Pap" which contains 32P-labeled mixed RNA probes complimentary to HPV types 6, 11, 16, 18, 31, 33, 35 DNA. Six of 18 cases in which HPV DNA were not detected by the Vira Pap were positive for HPV types 16 and/or 18, so that the PCR was a highly sensitive method compared to the Vira Pap. The PCR has some advantages: 1. it requires only a small amount of specimens and 2. paraffin-embedded sections can also be used. We suggest that the PCR is a useful method for the screening and for retrospective investigation of HPV infection.
    No preview · Article · Oct 1991 · Nippon Sanka Fujinka Gakkai zasshi
  • H Fujimoto · N Nagai · H Kioka · S Ohta · H Tanimoto · A Fujiwara
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    ABSTRACT: Human papillomavirus (HPV) types 16 and 18 have been found closely associated with squamous cell carcinoma and related lesions of the uterine cervix. In order to investigate the relationship between HPV and adenocarcinoma and adenosquamous carcinoma of the uterine cervix, formalin-fixed, paraffinembedded tissues prepared from 38 cases consisting of 30 cases of adenocarcinoma and 8 cases of adenosquamous carcinoma were examined for the presence of HPV DNA by in situ hybridization with digoxigenin labeled HPV 6/11, 16, 18 DNA probes. HPV DNA was localized on the nuclei of the cancer cells in adenocarcinoma and adenosquamous carcinoma. HPV DNA was detected in 13 cases (43.3%) of adenocarcinoma and 4 cases (50.0%) of adenosquamous carcinoma, and HPV type 18 DNA was detected in 13 cases (34.2%) of adenocarcinoma and adenosquamous carcinoma. These findings suggest an association between HPV, especially HPV type 18, and adenocarcinoma and adenosquamous carcinoma of the uterine cervix.
    No preview · Article · Jul 1991 · Nippon Sanka Fujinka Gakkai zasshi
  • N Nagai · H Kioka · S Ohta · H Fujimoto · H Tanimoto · T Fujii · A Fujiwara
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    ABSTRACT: We investigated human papillomavirus (HPV) DNA of vulvar diseases obtained from 18 cases of condyloma acuminatum, 3 cases of hyperplastic dystrophy without atypia, 4 cases of lichen sclerosus, 4 cases of Bowenoid papulosis, one case of Bowen disease and 8 cases of squamous cell cancer by in situ hybridization with biotinylated HPV 6, 11, 16 and 18 DNA probes. The results of in situ hybridization analysis showed that HPV 6/11 was positive in 94.4% (17/18) of condyloma acuminatum. In 17 cases of HPV 6/11 positive condyloma acuminatum, 3 cases were positive for HPV 16 and one for HPV 18, respectively. HPV 16 was positive in 75.0% (3/4) of Bowenoid papulosis and one case of Bowen disease was positive for HPV 18. In two cases of Bowenoid papulosis which were positive for HPV 16, cervical carcinoma in situ containing HPV 16 DNA sequences was also found during the follow up period. In 8 cases of squamous cell cancer, 2 cases were positive for HPV 18, one for HPV 16 and one for HPV 6/11. All cases of hyperplastic dystrophy without atypia and lichen sclerosus were negative for HPV DNA. Our results suggested HPV is closely associated with vulvar neoplasia.
    No preview · Article · Aug 1990 · Nippon Sanka Fujinka Gakkai zasshi
  • N Nagai · S Ohta · H Fujimoto · H Tanimoto · H Kioka · A Fujiwara

    No preview · Article · Jul 1990 · Nippon Sanka Fujinka Gakkai zasshi
  • H Kioka · N Nagai · Y Tanioka · T Fujii · Y Katsube · K Egawa · A Fujiwara
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    ABSTRACT: Cytological, histological, and molecular biological studies were conducted in 3 cases of vulvar Bowenoid papulosis, using biotinylated HPV DNA probes by in situ hybridization. 1) Cytological findings showed dyskaryotic cells that revealed hyperchromatism with a coarse granular pattern, and a high N/C ratio was observed among the dyskeratotic cells. 2) In 2 cases of Bowenoid papulosis lesions, HPV 16 DNA was detected in the nucleus of the dysplastic cells. 3) In one case of Bowenoid papulosis, a complicated carcinoma in situ of the uterine cervix was observed, and the HPV 16 DNA was found to be positive in both the vulva and cervix.
    No preview · Article · Oct 1989 · Gan no rinsho. Japan journal of cancer clinics
  • K Inai · K Arihiro · S Tokuoka · Y Katsube · A Fujiwara
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    ABSTRACT: Two cases of mesonephric duct hyperplasia of the uterus, together with three cases of uterine mesonephric duct remnant, are reported. The latter cases were identified by serial transverse sectioning of 42 surgically resected uteri, yielding a frequency of approximately 7%. Histochemical studies showed no mucin in the cytoplasm of cells lining the mesonephric duct remnant, as well as mesonephric duct hyperplasia. In contrast, the endocervical epithelium had abundant neutral mucin, sialomucin and sulfomucin. Immunohistochemical studies on lectin binding showed positive binding of PNA and SBA lectins only to the lining cells of mesonephric duct hyperplasia.
    No preview · Article · Aug 1989 · Acta pathologica japonica
  • Y Katsube · H Fujiwara · Y Tanioka · M Imajo · A Fujiwara
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    ABSTRACT: A case of ovarian clear cell adenofibroma of borderline malignancy in a 59-year-old woman is presented with histochemical and electron microscopic observation. The tumor was histologically composed of tubular component lined in part with flat or cuboidal clear as well as hobnail cells with mild cytologic atypia and abundant stromal component containing foci of calcification. The epithelial cells had diastase-digestive PAS-positive material in the cytoplasm, and occasionally the luminal surface and intraluminal substance were stained with mucicarmine. Some of the epithelial cells showed positive lipid staining with Sudan III stain. On ultrastructural study, the tumor featured a moderate number of small mitochondria, poorly developed rough endoplasmic reticulum (rER), short microvilli, intercellular tight junction and desmosome and small amount of glycogen granules. The patient is alive and well with no evidence of recurrence six and half years after the operation.
    No preview · Article · Jul 1989 · Hiroshima journal of medical sciences
  • N Nagai · H Kioka · K Shigemasa · A Fujiwara
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    ABSTRACT: Human papillomavirus (HPV) types 16 and 18 have been found closely associated with cervical cancer. In order to investigate the relationship between HPV DNA and cervical precancerous lesions, we examined the formalin fixed specimens obtained from 22 cases of mild dysplasia, 33 cases of moderate dysplasia and 31 cases of severe dysplasia of the uterine cervix for the presence of HPV 6/11, 16 and 18 DNAs by in situ hybridization using the biotinylated HPV DNA probes. We also followed some HPV DNA positive cases of cervical dysplasia for more than 6 months prospectively. The results of in situ hybridization analysis revealed that HPV DNA was detected in the nuclei of koilocytosis, dysplastic cells and metaplastic cells. HPV 6/11 was positive in 27.3% (6/22) of mild dysplasia and 21.2% (7/33) of moderate dysplasia. On the other hand, HPV 16 positive rate increased with the grade of dysplasia and 36.4% (12/33) of moderate dysplasia, 61.3% (19/31) of severe dysplasia were positive for HPV 16 DNA. Some of the follow-up cases which were positive for HPV 16 DNA were later found to have carcinoma in situ. Our results suggest that HPV type 16 might play an important role in cervical carcinogenesis.
    No preview · Article · Apr 1989 · Nippon Sanka Fujinka Gakkai zasshi
  • H Myoga · M Tamaki · Y Shinko · S Ohta · K Shigemasa · T Nogawa · Y Katsube · A Fujiwara
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    ABSTRACT: A first case of synchronous double cancers in the ovary and kidney is presented. The patient is a 37-year-old woman complaining of an abdominal mass. Histopathological findings showed a mucinous cystadenocarcinoma of the right ovary and a renal cell carcinoma, common type, clear cell subtype of the right kidney. A metastatic renal cell carcinoma was found on the pelvic peritoneum and characteristic renal cancer cells were identified in the ascitic fluid. (Evaluations: ovarian cancer, stage Ia; renal cancer, stage IV) The patient received postoperative chemotherapy and, after recovery, was discharged in a healthy condition. Her 11-month postoperative evaluation revealed no evidence of disease.
    No preview · Article · Dec 1988 · Gan no rinsho. Japan journal of cancer clinics
  • N Nagai · H Kioka · K Shigemasa · A Fujiwara · T Hozumi

    No preview · Article · Oct 1988 · Nippon Sanka Fujinka Gakkai zasshi
  • N Nagai · H Kioka · K Shigemasa · Y Shinko · A Fujiwara
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    ABSTRACT: In order to investigate the relationship between the presence of human papillomavirus (HPV) DNA and cervical carcinoma, we examined the cervical screening cells as well as the biopsy specimens obtained from 3 cases of severe dysplasia, 13 cases of carcinoma in situ (CIS) and 2 cases of microinvasive carcinoma for the presence of HPV types 6, 11, 16 and 18 DNA by DNA-DNA in situ hybridization using the biotinylated HPV DNA probes. The results of in situ hybridization analysis revealed that HPV 16 DNA sequences were detected in the nuclei of koilocytosis of severe dysplasia and CIS cases. The nuclei of atypical cells obtained from cervical screening cells were positive for HPV 16 or 18 DNA sequences. Two CIS cases were positive for the presence of HPV 16 and 18 DNA sequences. None of them contained HPV 6/11 DNA sequences. Eighteen cervical screening cases were examined and 10 contained HPV 16 DNA sequences and 6 contained HPV 18 DNA sequences. We suggest that the identification of HPV DNA types in cervical screening cells by in situ hybridization might be of diagnostic and prognostic value in early cervical neoplasia.
    No preview · Article · Oct 1988 · Nippon Sanka Fujinka Gakkai zasshi
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    ABSTRACT: The first case of ovarian sclerosing stromal tumor associated with endometrial adenocarcinoma in a 44-year-old woman is presented. By ultrastructural study, typical steroid-secreting type theca cells and various transitional tumor cells from fibroblast-like cells to theca-like cells were observed in the ovarian tumor. The clinical history of the patient and the ultrastructural study of this tumor suggested hormonal activity, but no apparent evidence of active hormone secretion was detected by plasma analysis for steroid hormones.
    No preview · Article · Apr 1988 · Gynecologic Oncology
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    ABSTRACT: 卵巣漿液性嚢胞腺癌10例を対象として, 酵素抗体法を用いたCA125およびEMAの免疫染色を行い, それらの組織局在および腹水中癌細胞, 中皮細胞, 組織球における染色性を検討した. また対照として非癌症例の腹水中中皮細胞におけるCA125およびEMAの染色性もあわせ検討し, 以下の結果を得た.(1) 摘出組織において, CA125およびEMAはそれぞれ90%(9/10), 100%(10/10) とともに高率に陽性所見が認められた.(2) 腹水中癌細胞においては, CA125およびEMAはともに10例全例 (100%) で陽性所見が観察され, またCA125-EMA二重染色によりCA125はEMAと比較し高率に癌細胞を検出し得る可能性が示唆された.(3) 癌症例および非癌症例における腹水中中皮細胞でのCA125の陽性率はともに高率であり, 一部の活動性中皮細胞においては癌細胞類似の染色性が観察された. 一方, EMAはごく一部の中皮細胞と反応するのみであり, また活動性中皮細胞における染色性も癌細胞と比較すると明らかに微弱であった.(4) 腹水中組織球においては, CA125, EMAともに陽性所見を認めなかった.以上の結果, CA125, EMAの両者を組みあわせて腹水細胞診に応用することにより, 原発巣の推定や良・悪性の鑑別の補助として利用し得ることが期待された.
    No preview · Article · Jan 1988

  • No preview · Article · Jan 1988
  • H Myohga · K Date · S Ohta · Y Katsube · H Itoh · A Fujiwara

    No preview · Article · Jul 1987 · Hiroshima journal of medical sciences
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    ABSTRACT: A case of carcinosarcoma, mixture of endometrial stromal sarcoma and adenocarcinoma, of the uterus with alpha-fetoprotein (AFP) production in a 69-year-old woman was reported. By the results of serum AFP levels (preoperative: 2,950.0 ng/ml, post-operative and chemotherapy: 2.1 ng/ml) and immunohistochemical localization of AFP, the AFP was thought to be produced from this tumor. Immunoreactive AFP was found in the carcinomatous components and no AFP was present in the sarcomatous components at all. It seems from this that immunohistochemical study supports the composition theory of mixed mullerian sarcoma of the uterus.
    No preview · Article · Mar 1987 · Gan no rinsho. Japan journal of cancer clinics

  • No preview · Article · Jan 1987
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    ABSTRACT: 子宮頸部原発carcinoid腫瘍は比較的少なく, その細胞診, 組織診, 電顕像についての総合的な報告はまだわずかである.今回われわれは, 細胞診で頸部腺癌と考え, 摘出物の組織診でcarcinoid腫瘍と判明した症例を経験したので報告する.症例は31歳の主婦で, 接触出血を訴え受診初診時の内診で子宮腟部前唇に栂指頭大の易出血性ポリープ状腫瘤を認め, 子宮頸癌Ib期の診断のもとに拡大単純子宮全摘術を施行した.子宮頸部擦過細胞診では, 腫瘍細胞は散在性ないし集塊状に出現していた.核は比較的小型で類円形から楕円形を示し, 大小不同は軽度で核縁は比較的平滑, 肥厚も著明でなく, 顆粒状ないし細顆粒状のchromatin patternを認め, 核小体は1ないし数個認めたが特徴的ではなかった.腫瘍細胞のN/C比は大きく, 細胞質はlight greenで比較的少なく細胞境界は不明瞭で, 頸部腺癌と判定した.摘出物の病理組織像は, H-E染色で好酸性の胞体をもつ小型異型細胞が充実性胞巣状に増殖し, rosette形成を認めた、Masson-Fontana染色は陰性であったが, Grimelius染色では細胞質内に微細な好銀顆粒が染色され, 電顕での検索で限界膜を有する神経分泌顆粒が認められ, 子宮頸部原発の低分化型carcinoid腫瘍と診断された.
    No preview · Article · Jan 1986
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    ABSTRACT: A case of a vaginal hemangiopericytoma in a 20-year-old woman was studied by light and electron microscopy. The neoplastic cells had round-to-oval nuclei with one or two nucleoli; fine chromatin; a foamy, cyanophilic cytoplasm; and an increased nuclear cytoplasmic ratio. Light microscopically, the tumor was composed of spindle-shaped or round cells proliferating around vascular spaces. Mitotic figures, necrosis, and hemorrhage were difficult to find. Electron microscopically, the neoplastic cells occurred in clusters, each of which was enclosed by a basal lamina. The cytoplasm contained abundant free ribosomes, flattened elements of rough endoplasmic reticulum, small Golgi apparatus, round or elongated mitochondria, lysosomes, glycogen particles, and sometimes pinocytotic vesicles and bundles of microfilaments with large dense spots. Desmosomes were also seen. In addition, the cytoplasmic process of the benign vascular pericytes was directly in contact with the neoplastic cells which had varying degrees of cytodifferentiation. The neoplastic cells in this lesion are consistent with an origin from pericytes, confirming the findings of this neoplasm when it arose in other sites.
    No preview · Article · Aug 1985 · Gynecologic Oncology
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    ABSTRACT: During the period between 1972 and 1978, 226 patients were operated on for carcinoma of the uterine cervix at Hiroshima University, and 91 patients were treated by postoperative external irradiation. Patients with lymph node metastasis showed markedly lower five-year survival rates stage I: 44.4%; stage II: 45.0%) than those without lymph node metastasis (99.3% and 84.0%, respectively). In view of benefits of postoperative radiotherapy in the treatment of cervical cancer, only to the node positive and tumor rest patients is recommended.
    No preview · Article · May 1985 · Gan no rinsho. Japan journal of cancer clinics

Publication Stats

45 Citations
11.32 Total Impact Points

Institutions

  • 1981-1990
    • Hiroshima University
      • Department of Obstetrics and Gynecology
      Hirosima, Hiroshima, Japan
  • 1988
    • George Washington University
      Washington, Washington, D.C., United States