Li Li

Changhai Hospital, Shanghai, Shanghai, Shanghai Shi, China

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Publications (42)106.76 Total impact

  • Li Li · Jianbo Liu · Xiaohai Yang · Jin Huang · Dinggeng He · Xi Guo · Lan Wan · Xiaoxiao He · Kemin Wang
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    ABSTRACT: Amino acid-dithiocarbamate (amino acid-DTC) was developed as both the reductant and ligand stabilizer for biomimetic synthesis of gold nanoparticles (AuNPs), which served as an excellent surface-enhanced Raman scattering (SERS) contrast nanoprobe for cell imaging. Glycine (Gly), glutamic acid (Glu), and histidine (His) with different isoelectric points were chosen as representative amino acid candidates to synthesize corresponding amino acid-DTC compounds through mixing with carbon disulfide (CS2), respectively. The pyrogenic decomposition of amino acid-DTC initiated the reduction synthesis of AuNPs, and the strong coordinating dithiocarbamate group of amino acid-DTC served as a stabilizer that grafted onto the surface of the AuNPs, which rendered the as-prepared nanoparticles a negative surface charge and high colloidal stability. MTT cell viability assay demonstrated that the biomimetic AuNPs possessed neglectful toxicity to the human hepatoma cell, which guaranteed them good biocompatibility for biomedical application. Meanwhile, the biomimetic AuNPs showed a strong SERS effect with an enhancement factor of 9.8 × 10(5) for the sensing of Rhodamine 6G, and two distinct Raman peaks located at 1363 and 1509 cm(-1) could be clearly observed in the cell-imaging experiments. Therefore, biomimetic AuNPs can be explored as an excellent SERS contrast nanoprobe for biomedical imaging, and the amino acid-DTC mediated synthesis of the AuNPs has a great potential in bio-engineering and biomedical imaging applications.
    No preview · Article · Feb 2016 · Nanotechnology
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    ABSTRACT: Multidrug resistance (MDR) in epithelial ovarian cancer (EOC) remains a public health issue for women worldwide, and its molecular mechanisms remain to be fully elucidated. The present study aimed to predict the potential genes involved in MDR, and examine the mechanisms underlying MDR in EOC using bioinformatics techniques. In the present study, four public microarray datasets, including GSE41499, GSE33482, GSE15372 and GSE28739, available in Gene Expression Omnibus were downloaded, and 11 microRNAs (miRNA; miRs), including miR‑130a, miR‑214, let‑7i, miR‑125b, miR‑376c, miR‑199a, miR‑93, miR‑141, miR‑130b, miR‑193b* and miR‑200c, from previously published reports in PubMed were used to perform a comprehensive bioinformatics analysis through gene expression analysis, signaling pathway analysis, literature co‑occurrence and miRNA‑mRNA interaction networks. The results demonstrated that the expression of neuropilin 1 (NRP1) was upregulated, thereby acting as the most important hub gene in the integrated gene network. NRP1 was targeted by miR‑130a and miR‑130b at the binding site of chromosome 10: 33466864‑3466870, which was involved in the axon guidance signaling pathway. These results suggested that alteration of the gene expression levels of NRP1 expression may contribute to MDR in EOC. These data provide important information for further experimental investigations of the drug resistance‑associated functions of NRP1 in EOC.
    No preview · Article · Nov 2015 · Molecular Medicine Reports
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    ABSTRACT: Methionine synthase reductase (MTRR) is involved in the DNA synthesis and production of S-adenosylmethionine (SAM) and plays an important role in the carcinogenesis. However, the role of MTRR in the resistance of ovarian cancer (OC) to chemotherapy has yet to be elucidated. In order to investigate the clinical significance of MTRR in OC, MTRR expression was reduced by using the RNA interference technique, and therefore, and the tumor growth and cisplatin-resistance were evaluated in vitro and in vivo. Results showed MTRR expression increased orderly from normal tissues, benign ovarian tumor to OC tissue. MTRR over-expression in OC tissue was correlated with pathologic type (P=0.005), grade (P=0.037), FIGO stage (P=0.001), organ metastasis (P=0.009) and platinum resistance (P=0.038). MTRR silencing inhibited cell proliferation, cisplatin resistance and autophagy, and induced apoptosis of OC cells. In addition, MTRR silencing also affected the caspase expression as well as mTOR signaling pathway. Further, the tumor volume in MTRR-suppressed SKOV3/DDP mice treated with cisplatin significantly decreased when compared with controls (P<0.05). In summary, MTRR expression, which is increased in human OC, is related to the differentiation and cisplatin resistance of OC cells. MTRR silencing inhibits cell growth and cisplatin resistance by regulating caspase expression and mTOR signaling pathway in OC cells. It is suggested that MTRR may be a potential target for the therapy of OC.
    No preview · Article · Nov 2015 · American Journal of Translational Research
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    ABSTRACT: Poor response to clopidogrel is often associated with recurrent ischemic events, and reliable platelet function tests are needed to identify clopidogrel low response (CLR). The aim of the study was to compare the consistency of VerifyNow P2Y12 and thrombelastography (TEG) in acute ischemic stroke patients treated with clopidogrel. Patients hospitalized in Changhai Hospital from August 2012 to September 2013 and assigned to treatment with a daily 75-mg dose of clopidogrel. The blood samples were taken on the 5–7th day to assess the capability of VerifyNow P2Y12 and TEG for evaluation of clopidogrel response, and all instrument parameters were used to perform correlation analysis. Patients with CLR were detected by using the methods and criteria published earlier (PRU ≥ 230 assayed by VerifyNow P2Y12 or TEG-Inhib% ≤30 % measured by TEG). Totally 58 patients were enrolled for the study and there were wide varieties in parameters of VerifyNow P2Y12 and TEG. Results showed a total of 17 and 9 patients, respectively, identified as CLR assessed by VerifyNow P2Y12 and TEG, but only three patients were detected to be clopidogrel low responders with both tests. The kappa consistency analysis showed poor consistency between VerifyNow P2Y12 and TEG results in terms of CLR (Kappa = −0.0349, p = 0.7730). Linear regression also demonstrated poor correlation between VerifyNow-PRU/VerifyNow-Inhib% and TEG-Inhib% (p = 0.07901 and p = 0.3788, respectively). Our study demonstrated that there was poor correlation between VerifyNow P2Y12 and TEG results, and VerifyNow P2Y12 showed a larger proportion of CLR than TEG.
    No preview · Article · Oct 2015 · Neurological Sciences
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    ABSTRACT: Taking beta-cyclodextrin (β-CD) as reducting agent and dispersant, PtRh nanoparticles with varied metal composition were uniformly deposited on the β-CD functionalized carbon nanotubes (β-CD-CNTs) by one-pot hydrothermal synthesis method. Fourier transform infrared spectroscopy and thermogravimetric analysis were used to demonstrate the successful modification of CNTs with β-CD. The morphology, chemical composition and structure of the β-CD-CNTs supported PtRh catalysts (PtRh/β-CD-CNTs) have been characterized by transmission electron microscopy, X-ray diffraction and energy dispersive X-ray spectroscopy, respectively. Their electrochemical performances were investigated by typical electrochemical technologies. The results indicate that PtRh/β-CD-CNTs catalyst with 1:1 atomic ratio of Pt/Rh (Pt1Rh1/β-CD-CNTs) shows superior electrocatalytic properties towards methanol oxidation. In addition, compared with the acid-treatment CNTs (AO-CNTs) supported PtRh nanoparticles (PtRh/AO-CNTs) prepared by typical NaBH4 reduction, Pt1Rh1/β-CD-CNTs exhibits more excellent electrocatalytic performance for methanol electro-oxidation due to the smaller particle size and more uniform deposition of PtRh nanoparticles.
    No preview · Article · Oct 2015 · International Journal of Hydrogen Energy
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    ABSTRACT: Here we present a novel large Stokes shifting NIR fluorescent nanomicelle through encapsulation of quantum dot/ methylene blue FRET pair, which is employed as an excellent contrast reagent for NIR fluorescent bioimaging.
    No preview · Article · Aug 2015 · Chemical Communications
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    ABSTRACT: Nitrogen-doped porous carbon micropolyhedra (N-PCMPs) were successfully prepared by direct carbonization of ZIF-11 polyhedra and further activated with fused KOH to obtain N-PCMPs-A. The morphology and microstructure of samples were examined by scanning electron microscopy, X-ray diffraction, and micropore and chemisorption analyzer. Electrochemical properties were characterized by cyclic voltammetry and galvanostatic charge/discharge method in 1.0 M H2SO4 aqueous solution on a standard three-electrode system. Results show that, compared with N-PCMPs, N-PCMPs-A has higher specific surface area (2188 m2 g−1) and exhibits improved electrochemical capacitive properties (307 F g−1 at 1.0 A g−1). The mass specific capacitance of N-PCMPs-A is also higher than that of most MOF-derived carbons, some carbide-derived carbons and carbon aerogel-derived carbons. In addition, the capacitance of the N-PCMPs-A retains 90% after 4000 cycles even at a high current density of 10 A g−1. These imply that N-PCMPs-A is the promising materials for the construction of a high-performance supercapacitor.
    Full-text · Article · Jun 2015 · Materials Research Bulletin
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    ABSTRACT: A new Pt-based electrocatalyst with one-dimensional tubular Mn3O4-C as the catalyst support was synthesized by a dual-sacrificial template strategy. The morphology, structure, and composition of the Pt-Mn3O4- C composite were characterized by transmission electron microscopy, X-ray diffraction, and energy dispersive X-ray spectroscopy, respectively. The electrochemical performance of Pt-Mn3O4-C was investigated by cyclic voltammetry. The results show that Pt nanoparticles with an average size of 1.8 nm are uniformly dispersed on tubular Mn3O4-C, and Pt-Mn3O4-C exhibits superior electrocatalytic activity and higher stability for methanol oxidation than the commercial E-TEK Pt/C catalyst (20% (w, mass fraction) Pt). The excellent performance of Pt-Mn3O4-C is attributed to the uniform dispersion of Pt nanoparticles on Mn3O4-C and the synergetic catalytic effect of Pt and Mn3O4.
    No preview · Article · May 2015 · ACTA PHYSICO-CHIMICA SINICA
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    ABSTRACT: Taking malic acid as the carbon source, carbon coated-MnO2 nanorods (MnO2@C NRs) were prepared through solid-state grinding/low-temperature calcining synthesis method. The morphology, structure and electrochemical capacitive property of MnO2@C NRs were characterized by transmission electron microscopy, scanning electron microscopy, X-ray diffraction, surface area and pore size analyzer and electrochemical methods. The results demonstrate that the thickness of the carbon layer on the surface of MnO2 NRs is about 3 nm and the MnO2@C NRs have improved capacitive performance and excellent long-term cycling stability. This work provides a facile route with energy savings, cost effectiveness, environmental-friendliness and large-scale production ability in the synthesis of carbon-coated metal oxide nanomaterials.
    No preview · Article · Apr 2015 · New Journal of Chemistry
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    ABSTRACT: A novel exciton energy transfer-based fluorescence sensing array for the discrimination of different nucleobases was developed through target nucleobase-triggered self-assembly of quantum dots (QDs). Four QD nanoprobes with different ligand receptors, including mercaptoethylamine, N-acetyl-L-cysteine, 2-dimethyl-aminethanethiol, and thioglycolic acid, were created to detect and identify nucleobase targets. These QDs served as both selective recognition scaffolds and signal transduction elements for biomolecule target. The extent of particle assembly, induced by the analyte-triggered self-assembly of QDs, led to an exciton energy transfer effect between interparticles that gave a readily detectable fluorescence quenching and distinct fluorescence response patterns. These patterns are characteristic for each nucleobase and can be quantitatively differentiated by linear discriminate analysis. Furthermore, a fingerprint-based barcode was established to conveniently discriminate the nucleobases. This pattern sensing was successfully used to identify nucleobase samples at unknown concentration and five rare bases. In this "chemical noses" strategy, the robust characteristics of QD nanoprobes, coupled with the diversity of surface functionality that can be readily obtained using nanoparticles, provides a simple and label-free biosensing approach that shows great promise for biomedical applications.
    No preview · Article · Dec 2014 · Analytical Chemistry
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    ABSTRACT: Due to their high loading capacity, permeability modulation, and biological compatibility, mesoporous silica spheres provide a suitable system for mimicking cellular compartments. Herein, a surface amine group functionalized mesoporous silica sphere was developed as a biomimetic compartment model, in which the ion permeability could be well modulated through the external phosphate ion. The amine group was selectively modified on the surface of the mesoporous silica sphere in the presence of a template molecule (CTAB) in the mesochannel. The surface amine group was employed as a gatekeeper shell and the specific binding of the anionic phosphate with the amine groups reversed the surface charge from positive to negative and gated the permeability of the model cationic Ru(bipy)32+. Ion channel decorated compartment is one of the key architectual principles of the cell, which maintains a high local reagent concentration inside it via the high surface area interior, and its permeability is tuned by a collection of ligand-gated ion channels outside to ensure a metabolic balance. The permeability modulation in the mesoporous silica sphere closely resembles that observed in a biological ion channel decorated compartment in vivo. This protocol provides the possibility of simulating the process of ion permeability in biological compartments based on mesoporous silica spheres, and it also contributes to the design of artificial cells and bio-inspired responsive nanoreactors.
    No preview · Article · Dec 2014
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    ABSTRACT: Platinum nanoparticles (Pt NPs) encapsulated in nitrogen-doped mesoporous carbons (NMCs) are prepared by direct carbonization of zeolitic imidazolate framework-8 (ZIF-8)-encapsulated Pt NPs, and used as a methanol-tolerant oxygen reduction electrocatalyst. ZIF-8 is used as the carbon and nitrogen precursors and as a matrix for Pt NPs. The obtained Pt NP–NMC hybrids are characterized in detail. The results show that the Pt NP–NMC hybrids possess high surface area (1226 m2 g−1), abundant mesopores with narrow pore size distribution (centered at 3.9 nm), nitrogen doping (5.13 at %), and small and well-dispersed encapsulated Pt NPs (3.7 nm). Furthermore, the prepared Pt NP–NMC catalyst exhibits high electrocatalytic activity, high stability and excellent methanol tolerance in the oxygen reduction reaction.
    No preview · Article · Dec 2014
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    ABSTRACT: A series of novel tetramethylguanidinium ionic liquids and hexaalkylguanidinium ionic liquids have been synthesized based on 1,1,3,3-tetramethylguanidine. The structures of the ionic liquids were confirmed by 1H NMR spectroscopy and mass spectrometry. A green guanidinium ionic liquid based microwave-assisted extraction method has been developed with these guanidinium ionic liquids for the effective extraction of Praeruptorin A from Radix peucedani. After extraction, reversed-phase high-performance liquid chromatography with UV detection was employed for the analysis of Praeruptorin A. Several significant operating parameters were systematically optimized by single-factor and L9 (34) orthogonal array experiments. The amount of Praeruptorin A extracted by [1,1,3,3-tetramethylguanidine]CH2CH(OH)COOH is the most, reaching 11.05 ± 0.13 mg/g. Guanidinium ionic liquid based microwave-assisted extraction presents unique advantages in Praeruptorin A extraction comparing with guanidinium ionic liquid based maceration extraction, guanidinium ionic liquid based heat reflux extraction and guanidinium ionic liquid based ultrasound-assisted extraction. The precision, stability and repeatability of the process were investigated. The mechanisms of guanidinium ionic liquid based microwave-assisted extraction were researched by scanning electron microscopy and IR spectroscopy. All the results show that guanidinium ionic liquid based microwave-assisted extraction has a huge potential in the extraction of bioactive compounds from complex samples.This article is protected by copyright. All rights reserved
    No preview · Article · Dec 2014 · Journal of Separation Science
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    ABSTRACT: The aim of this study is to examine the microRNA (miRNA) expression of epithelial ovarian cancer (EOC) in both drug-resistant and drug-sensitive tissues and to explore the pathogenic characteristics of drug-resistant miRNAs in EOC. The samples with 10 cases of drug-resistant and drug-sensitive EOC tissue were obtained from undergoing surgical resection of ovarian cancer (OC). Total miRNAs were extracted and isolated, respectively. Hybridization was carried out on miRNA microarray chip. Real-time polymerase chain reaction (RT-PCR) was performed to confirm the difference of miRNA expression. Bioinformatic software was used to predict the possible target genes of each miRNA which expressed differently. The results indicated that four miRNAs related drug-resistance been identified, and the expression of hsa-miR-152 and hsa-miR-381 in drug-resistant OC tissue was significantly higher compared with those in drug-sensitive tissue (P < 0.01). However, expression of hsa-miR-200a-3p and hsa-miR-429 were downregulated in drug-resistant tissues (P < 0.01). The results obtained by miRNA microarrays of differential expression with hsa-miR-106b-3p, hsa-miR-152, hsa-miR-200a-3p, hsa-miR-381, and hsa-miR-429 were confirmed by real-time PCR. There were 62 significantly different miRNAs, including 42 significant upregulated miRNAs and 20 significant downregulated miRNAs in the drug-resistant tissue. Five databases, including Target Scan, miRanda, miRDB, PicTar5, and RNA22, were used for bioinformatics prediction. In conclusion, miRNA microarray analysis has become a fast and efficient molecular biological technology for the study of biological information. hsa-miR-152, hsa-miR-200a-3p, hsa-miR-381, and hsa-miR-429 may participate in the formation of drug resistance in EOC through the target genes predicted.
    No preview · Article · May 2014 · Tumor Biology
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    ABSTRACT: Avian influenza virus H5 is a widespread virus among humans and animals which has caused fatally systemic diseases through poultry-to-person transmission in the past few years. Reverse transcription polymerase chain reaction (RT-PCR) has been proved to be an effective approach for the identification and detection of avian influenza viruses. However, conventional tube RT-PCR is slow and reagent consuming and cannot meet the need for rapid and low cost detection of pathogenic bacteria and viruses. Microfluidic PCR is a burgeoning field among the techniques based on molecular analysis. In this paper, we reported a microfluidic PCR system that integrated RT-PCR and real time fluorescence detection for rapid identification of avian influenza virus H5. This microfluidic device mainly consisted of a thermal controlling unit providing actuation for the temperature cycling needed for amplification, an optical inspection system for online recording fluorescence and a microfluidic chip fabricated using polydimethylsiloxane (PDMS). In this study, influenza virus H5 from clinical chicken throat swab specimens was rapidly detected using the RT-PCR microfluidic system, which was consistent with the results of embryonated egg culture. Compared with a large-scale device, the integrated microfluidic system presented here can perform rapid nucleic acid amplification and analysis, possibly making it a crucial platform for pathogenic bacterium and virus detection in the future.
    No preview · Article · Apr 2014 · Analytical methods
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    ABSTRACT: NEK2 [NIMA (never in mitosis gene A)-related expressed kinase 2] is associated with various biological behaviors in the development of cancer, while research concerning its association with drug resistance is limited. The association of NEK2 with drug resistance in ovarian cancer has not yet been reported. In the present study, on the basis of microarray results from Oncomine and the GEO Profiles online database, we revealed that NEK2 mRNA expression in ovarian cancer tissues is upregulated. In addition, its expression in drug-resistant ovarian cancer cells was upregulated when compared with expression with their sensitive or parental counterparts. Finally, we performed a comprehensive bioinformatic analysis consisting of protein/gene-protein/gene interaction network, annotation of biological processes and microRNA-mRNA interaction analysis. We observed that NEK2 directly or indirectly interacts with a number of genes, proteins, microRNAs and biological processes associated with drug resistance in ovarian and other types of cancer. These results indicate that NEK2 contributes to drug resistance in ovarian cancer and it may be an important therapeutic target.
    Preview · Article · Dec 2013 · Oncology Reports
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    ABSTRACT: A novel exciton energy transfer-based ultrasensitive fluorescent sensing strategy for the detection of biological small molecule and protein has been established through split aptamer- programmed self-assembly of quantum dots (QDs). The signal is produced from exciton energy transfer of the self-assembled QDs. The recognition is accomplished using an aptamer sensor scaffold designed with two split fragment sequences, which specifically bind to the model analytes. The extent of particle assembly, induced by the analyte-triggered self-assembly of QDs, leads to an exciton energy transfer effect between interparticles, giving a readily detectable fluorescent quenching and redshift of the emission peak, which enables us to quantitate the target in dual signal modes. The application of the technique is well demonstrated using two representative split aptamer- based model systems for the detection of adenosine and thrombin. The sensitivity of this exciton energy transfer-based fluorescent sensing is much better than that of plasmonic coupling-based colorimetric methods. Limit of detections (LODs) down to 12 nM and 15 pM can be achieved for adenosine and thrombin, respectively. The sensing strategy is proposed as a general platform for robust and specific aptamer-target analysis which could be further developed to monitor a wide range of target analytes. The concept and methodology developed in this work shows a good promise in the study of molecular binding events in the biological and medical applications.
    No preview · Article · Oct 2013 · Analytical Chemistry
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    ABSTRACT: Sex hormones and gonadotropins exert a wide variety of effects in physiological and pathological processes. Accumulated evidence shows a strong heritable component of circulating concentrations of these hormones. Recently, several genome-wide association studies (GWASs) conducted in Caucasians have identified multiple loci that influence serum levels of sex hormones. However, the genetic determinants remain unknown in Chinese populations. In this study, we aimed to identify genetic variants associated with major sex hormones, gonadotropins, including testosterone, oestradiol, follicle-stimulating hormone (FSH), luteinising hormone (LH) and sex hormone binding globulin (SHBG) in a Chinese population. A two-stage GWAS was conducted in a total of 3495 healthy Chinese men (1999 subjects in the GWAS discovery stage and 1496 in the confirmation stage). We identified a novel genetic region at 15q21.2 (rs2414095 in CYP19A1), which was significantly associated with oestradiol and FSH in the Chinese population at a genome-wide significant level (p=6.54×10(-31) and 1.59×10(-16), respectively). Another single nucleotide polymorphism in CYP19A1 gene was significantly associated with oestradiol level (rs2445762, p=7.75×10(-28)). In addition, we confirmed the previous GWAS-identified locus at 17p13.1 for testosterone (rs2075230, p=1.13×10(-8)) and SHBG level (rs2075230, p=4.75×10(-19)) in the Chinese population. This study is the first GWAS investigation of genetic determinants of FSH and LH. The identification of novel susceptibility loci may provide more biological implications for the synthesis and metabolism of these hormones. More importantly, the confirmation of the genetic loci for testosterone and SHBG suggests common genetic components shared among different ethnicities.
    Full-text · Article · Sep 2013 · Journal of Medical Genetics
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    ABSTRACT: Mesoporous silica nanoparticles (MSN) have emerged as appealing host materials to accommodate guest molecules for biomedical applications, and recently various methods have been developed to modulate the loading of guest molecules in the silica matrix. Herein, it was demonstrated that pH and ion strength showed great influence on the loading of charged species into the nanoparticles, taking MCM-41 as a host MSN model and methylviologen (MV(2+)) and 1,5-naphthalene disulfonate (NDS(2-)) as typical charged ionic guest molecules. As the pH increased from 3.0 to 8.0, the loading amount of MV(2+) increased gradually, while on the contrary, it decreased gradually for NDS(2-), for the solution pH changed the electrostatic interaction between the silica matrix and the ionic guest molecules. Additionally, the adding of NaCl reduced the electrostatic interaction, which resulted in a decreasing of the electrostatic rejection and electrostatic accumulation for the molecules carrying the same and the opposite charge to the particle respectively. Thus, pH and ion strength can be employed as simple approaches to modulate the loading of charged molecules and permselectivity in MSN. This work has a definite guidance function for molecule loading, transport modulation, controlled release as well as sensors based on MSN.
    No preview · Article · Sep 2013 · Nanotechnology
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    ABSTRACT: Increased serum uric acid (SUA) levels may be involved in the development of non-alcoholic fatty liver disease (NAFLD) in men presenting with metabolic syndrome (MetS) and/or insulin resistance. We aimed to determine the independent relationship between SUA and NAFLD in non-diabetic Chinese male population, and to explore the determinants of SUA levels among indexes of adiposity, lipid, and genotypes pertaining to triglycerides metabolism, inflammation, oxidative stress, and SUA concentrations. A total of 1440 men, classified depending on the presence of ultrasonographically detected NAFLD, underwent a complete healthy checkup program. Genotypes were extracted from our previously established genome-wide association study database. After adjusting for age, smoking, drinking, body mass index, homeostasis model assessment of insulin resistance, C-reactive protein, creatinine, alanine aminotransferase (ALT) and components of metabolic syndrome, the odds ratio for NAFLD, comparing the highest with the lowest SUA quartile, was 2.81 (95% confidence interval 1.66-4.76). A stepwise multivariate linear regression analysis (R(2) = 0.238, P<0.001) retained age, waist circumference, serum creatinine, triglycerides, the Q141K variant in ABCG2 (rs2231142) and NAFLD as significant predictors of SUA levels (all P<0.001). Besides, ALT and Met196Arg variant in TNFRSF1B (rs1061622) additionally associated with SUA among individuls with NAFLD. Our data suggest that in Chinese men, elevated SUA is significantly associated with NAFLD, independent of insulin resistance and other metabolic disorders, such as central obesity or hypertriglyceridemia. Meanwhile, among subjects with NAFLD, index of liver damage, such as elevated ALT combined with genetic susceptibility to inflammation associated with increased SUA levels.
    Full-text · Article · Jul 2013 · PLoS ONE

Publication Stats

222 Citations
106.76 Total Impact Points


  • 2015
    • Changhai Hospital, Shanghai
      Shanghai, Shanghai Shi, China
  • 2013-2015
    • Hunan University
      • College of Chemistry and Chemical Engineering
      Ch’ang-sha-shih, Hunan, China
  • 2011-2015
    • Guangxi Medical University
      • • School of Public Health
      • • Department of Gynecologic Oncology
      Yung-ning, Guangxi Zhuangzu Zizhiqu, China
    • Fudan University
      • Fudan-VARI Center for Genetic Epidemiology
      Shanghai, Shanghai Shi, China
  • 2012-2014
    • China Agricultural University
      • • College of Information and Electrical Engineering
      • • Key Laboratory of MOE on Modern Precision Agriculture System Integration Research
      Peping, Beijing, China
    • Nanchang University
      Nan-ch’ang-shih, Jiangxi Sheng, China
    • Guilin Medical University
      Ling-ch’uan, Guangxi Zhuangzu Zizhiqu, China