[Show abstract][Hide abstract] ABSTRACT: Campylobacter spp. may be responsible for unreported outbreaks of food-borne disease. The detection of these outbreaks is made more difficult
by the fact that appropriate methods for detecting clusters of Campylobacter have not been well defined. We have compared the characteristics of five molecular typing methods on Campylobacter jejuni and C. coli isolates obtained from human and nonhuman sources during sentinel site surveillance during a 3-year period. Comparative genomic
fingerprinting (CGF) appears to be one of the optimal methods for the detection of clusters of cases, and it could be supplemented
by the sequencing of the flaA gene short variable region (flaA SVR sequence typing), with or without subsequent multilocus sequence typing (MLST). Different methods may be optimal for
uncovering different aspects of source attribution. Finally, the use of several different molecular typing or analysis methods
for comparing individuals within a population reveals much more about that population than a single method. Similarly, comparing
several different typing methods reveals a great deal about differences in how the methods group individuals within the population.
Full-text · Article · Dec 2011 · Journal of clinical microbiology