Dominika Oleś

Poznan University of Medical Sciences, Posen, Greater Poland Voivodeship, Poland

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Publications (4)1.52 Total impact

  • Dominika Oleś · Aleksandra Szczepankiewicz
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    ABSTRACT: Toll-like receptors (TLR) are a family of receptors that play an important role in innate immune response. Their presence has been confirmed in macrophages, dendritic cells, neutrophils and mast cells. Toll-like receptors receptors recognize microbial products and their activation triggers the innate immune response mechanism. They can also participate indirectly in the adaptive immune response. Allergic asthma is a chronic inflammatory disease with characteristic clinical symptoms including airway hypersensitivity, airway obstruction and wheezing. Airway inflammation is related to eosinophilia, elevated levels of inflammatory mediators and excessive mucus secretion. In animal models it has been shown that Toll-like receptors, because of their role in immunological processes, may be an important therapeutic target in asthma.
    No preview · Article · Jan 2012
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    ABSTRACT: Introduction: Bronchial epithelial cell lines enable analysis of influence of different factors, such as exposure to inhaled antiasthmatic agents, on their morphology and function in vitro. Aim: To investigate whether chronic exposure to different concentrations of therapeutic agents used in the inhalation therapy of asthma influence bronchial epithelial cells growth and morphology. Material and methods: Bronchial epithelial cell line 16HBE14o- was used. We analyzed changes in viability between cells treated with different drug concentrations: budesonide, fluticasone propionate, salbutamol and ipratropium bromide. Observations were performed every 24 h for 4 days. Cells viability was analyzed by fluorescent staining and XTT assay. Results: An inverse correlation between drug concentration and cells viability was observed. The only exception was ipratropium bromide which was toxic at all studied concentrations. Steroids at the two highest concentrations led to a significant decrease in cells viability with fluticasone propionate being more potent than budesonide. Incubation with salbutamol also demonstrated decreased cells viability at the two highest concentrations. Ipratropium bromide was toxic for the bronchial cells at all concentrations leading to a significant decrease in cells viability. Conclusions: Chronic exposure to the highest concentrations of steroids or β2-agonists decreases viability of epithelial cells, whereas ipratropium bromide has the strongest influence on cell viability regardless of its concentration.
    No preview · Article · Jan 2012
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    ABSTRACT: Chromosomal imbalance in gametes and embryos is one of the factors contributing to early embryonic mortality. Although the rate of chromosomally abnormal sperm cells is low and usually does not exceed 1%, there is no clear indication of fertilizing potential of such gametes. The aim of the experiment was to investigate the type and incidence of numerical chromosomal aberrations in spermatozoa produced by fertile boars used in artificial insemination (AI). We used the protocol of fluorescent in situ hybridization (FISH) on sperm interphase nuclei with molecular probes for porcine chromosome pairs 1 and 10. Altogether 12 348 sperm cells were examined. Disomy was observed in spermatozoa of all seven AI boars whereas only one diploid cell was identified in all screened sperm cells. The average rate of chromosomally unbalanced sperm was 0.105% (13/12 348) with an inter-individual variation from 0.048% to 0.194%. Among abnormal sperm cells, both disomy (0.097%) and diploidy (0.008%) were detected. Nullisomy was not included into calculations. The estimated aneuploidy rate calculated by doubling the number of disomic cells was 0.194%. Chromosome pair 10 was significantly more often involved in non-disjunction (75%, 9/12 aneuploid sperm cells) than chromosome pair 1 (25%, 3/12). We have shown for the pig that the rate of disomic cells falls into a range presented by other authors, whereas that of diploid spermatozoa appeared to be lower in the present study. In conclusion, numerical chromosome aberrations were present in spermatozoa of all AI boars analyzed in this study. Therefore, it can be assumed that the presence of unbalanced spermatozoa at the level observed in fertile males does not significantly affect their reproductive potential.
    Preview · Article · Nov 2011 · Reproductive biology
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    ABSTRACT: Airway remodeling observed in asthmatic patients is a process involving the number of negative changes within the following structures: bronchial epithelium, bronchial miocytes, blood vessels and goblet cells. Remodeling contributes to adverse changes in the airways due to increased airflow resistance in the bronchi. Further effects depend on functional deterioration of lung function. Bronchial epithelial cells play an essential role in this process, because of their protective and secretory functions. Remodeling leads to the damage of epithelial cells by activating numerous factors (proteases, metaloproteinases, elastases), which in turn impairs the protective function of the epithelium. The use of inhaled drugs provides the direct action in the airways and increases the speed of action of these drugs. However, as treatment with inhaled drugs is related to their direct effect on bronchial epithelial cells, it is important to determine the influence of particular groups of drugs on the morphology and function of epithelial cells. The following paper describes changes in the airways observed during remodeling, and current knowledge on the influence of drugs on the airways' structure and function.
    No preview · Article · Mar 2011 · Alergia Astma Immunologia

Publication Stats

11 Citations
1.52 Total Impact Points

Institutions

  • 2012
    • Poznan University of Medical Sciences
      Posen, Greater Poland Voivodeship, Poland
  • 2011
    • Poznań University of Life Sciences
      • Department of Genetics and Animal Breeding
      Posen, Greater Poland Voivodeship, Poland