Sarah Thömmes

Heinrich-Heine-Universität Düsseldorf, Düsseldorf, North Rhine-Westphalia, Germany

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Publications (2)0.66 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed by SDS-PAGE of the outer membrane proteins, and the number of autodisplayed streptavidin molecules on a single E. coli cell was evaluated with densitometric analysis. The biotin-binding activity of the autodisplayed streptavidin was estimated after treatment with fluorescently labeled biotin by fluorescence microscopy and flow cytometry. The biotin-binding activity of the E. coli with autodisplayed streptavidin was compared with the activity of streptavidin immobilized on magnetic beads. Finally, the outer membrane presenting autodisplayed streptavidin was isolated and layered on a 96-well microplate for an immunoassay.
    No preview · Article · Apr 2011
  • Source
    S. Thömmes · F. Blasshofer · J. Jose

    Preview · Article · Sep 2010 · Chemie Ingenieur Technik

Publication Stats

10 Citations
0.66 Total Impact Points

Top Journals


  • 2010-2011
    • Heinrich-Heine-Universität Düsseldorf
      • Institute of Pharmaceutical and Medicinal Chemistry
      Düsseldorf, North Rhine-Westphalia, Germany