[Show abstract][Hide abstract] ABSTRACT: Thirty seven isolates of E. coli from broiler birds intestines and raw meat were serotyped and investigated for pathogenic attributes i.e. CR binding and haemolysin production. Serotyping of these isolates revealed 24 (64.8%) 'O' serotypes. The prominent serotype O2 (9) accounting for 37.5% was recovered from meat. Of all the strains tested, 18 (48.6%) showed CR binding ability. Prevalence of CR positive E. coli was higher in meat (13/65%) than in intestinal content (5/29.4%). However, in the present study none of the 37 isolates tested were haemolysin positive. Pathogenic attributes were revealed more in meat E. coli than intestinal E. coli of broiler birds. Present results indicated that the presence of virulent determinant in E. coli from broiler intestine and meat can have important implications to public health.
No preview · Article · Jan 2012 · The Indian veterinary journal
[Show abstract][Hide abstract] ABSTRACT: Biofilm formation in Staphylococcus aureus is considered an important virulence factor in bovine mastitis. Intercellular adhesion gene A (icaA) is a significant genetic determinant that contributes in biofilm formation. The aim of the present study was to determine the presence of the icaA gene in S. aureus isolated from bovine mastitis from seven states of India. A total of 88 out of 150 Staphylococcus aureus strains were found to be positive for biofilm marker icaA gene by PCR. The icaA gene was confirmed by dot blot hybridization in 41 of 150 S. aureus strains tested. Results obtained with dot blot hybridization were comparable to those obtained with PCR. Partial sequences of the icaA gene of the two S. aureus isolates showed deletion of some bases in different positions that might reduce/stop transcription leading to no biofilm formation. PCR was found to be a rapid test but dot blot hybridization was more accurate than PCR for detection of icaA genes. This study showed that detection of biofilm marker the icaA gene in S. aureus would allow the detection of virulence factors present in mastitis and early application of corrective measures.
No preview · Article · Nov 2011 · Tropical Animal Health and Production
[Show abstract][Hide abstract] ABSTRACT: A total of 294 serum samples were collected from apparently healthy goats of different age and sex from 13 tehsils of Nagpur district. All the samples were screened for Contagious Caprine Pleuro Pneumonia antibodies by slide agglutination test using colored CCPP antigen. Out of 294 serum samples screened 99 were found to be positive indicating overall seroprevalence of 33.67 per cent. The higher prevalence was observed in Saoner tehsil (44.44%) followed by Bhivapur (41.46%), Kalmeshwar (40.00 %) and Kamptee (38.46%). The higher incidence in these tehsils could be attributed to the presence of endemic foci in this area. Slide agglutination test for CCPP detection using colored antigen was found to be quick, simple, low cost with ease of application in the field without the need of any specialized training and equipments.
No preview · Article · Jan 2008 · Veterinary World