Huan Jiang

Jinan University (Guangzhou, China), Shengcheng, Guangdong, China

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Publications (10)9.1 Total impact

  • Qi-Zhi Liu · Wei-Jie Zhu · Hai-Peng Liu · Huan Jiang
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    ABSTRACT: Objective: To evaluate the level of antisperm antibody (ASA) in infertile women with chronic salpingtis. Methods: Forty-eight infertile women with chronic salpingtis were enrolled into this study. Serum samples were screened by the direct immunobead test for ASA type IgG and IgA according to the WHO laboratory manual. About 50% or more of the motile sperm attaching to one or more immunobeads were regarded as clinical positivity according to the WHO criteria. About 20%-50% motile sperm that had adherent particles were deemed to be sub-positive. Results: Of the 48 patients with chronic salpingtis assessed for ASA-IgG, 4 had immunobead bindings ranged from 50% to 60%, and the positive rate was 8.3%. In addition, 5 cases had 20%-40% of immunobead bindings, and the sub-positive rate was 10.4%. For ASA-IgA detecting, 2 cases with ASA-IgG positivity also had ASA-IgA immunobead bindings, and the positive rates were 22%, and 28%, respectively. Conclusion: The incidence of ASA could be found in infertile women with chronic salpingtis, which suggests that an increased risk for the production of ASA would exist in the inflammatory status of fallopian tube. © 2014 The Editorial Board of Journal of Reproduction and Contraception.
    No preview · Article · Sep 2014 · Journal of Reproduction and Contraception
  • Yi-chao Xu · Wei-jie Zhu · Huan Jiang
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    ABSTRACT: Objective To investigate the level of antisperm antibodies (ASA) in infertile men with absolute immotile sperm (AIS). Methods Thirteen men with AIS were enrolled into this study. Sperm immotility of ejaculates from these infertile men was proven by two or three consecutive semen analyses. Sperm vitality was assessed by the eosin exclusion. Serum and seminal plasma were screened by the indirect immunobead test (IBT) for ASA type IgG and IgA according to the WHO laboratory manual. Results Semen samples from these patients showed ejaculate volume, pH, and sperm count in the normal range. Sperm vitality, sperm concentration, and sperm abnormal forms in these cases were 40.2 ± 11.1%, 45.0 ± 11.9 × 106/ml, and 97.8 ± 1.4%, respectively. No case had the positive or sub-positive level of ASA type IgG and IgA in samples of both serum and seminal plasma. Conclusion ASA is not associated with the pathogenesis of AIS-associated infertility.
    No preview · Article · Jun 2014 · Journal of Reproduction and Contraception
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    ABSTRACT: To quantitatively assess the histological and ultrastructural changes resulting from aging in the human testis. Age-related histological and ultrastructural changes were evaluated using light microscopy, transmission electron microscopy (TEM) and immunohistochemistry on 41 testicular samples obtained from elderly men and, respectively, assigned to group A (n = 20), 54-69 years old or group B (n = 21), 70-89 years old. Testicular samples derived from 17 young men were used for control. The numbers of Sertoli cells in the aged groups were significantly lower than that in the controls (p < 0.05). With the exception of the Sertoli cell ratios (germ cells/Sertoli cells) of spermatogonia and primary spermatocytes, results showed lower levels of the Sertoli cell ratios of round spermatids and elongated spermatids in the elderly men compared with the young men (p < 0.05). A similar degenerative pattern of the organelles was shown in germ cells and Sertoli cells in the aging testes under TEM. Immunohistochemistry revealed an increased apoptosis index (AI) (0.81 ± 0.13) accompanied by a decreased proliferation index (PI) (30.08 ± 4.86) in the group B (p < 0.05), while both AI and PI were similar between the group A (0.54 ± 0.06; 36.38 ± 7.38) and the controls (0.50 ± 0.15; 40.55 ± 7.92) (p > 0.05). Aging has negative influence on testicular morphology and spermatogenesis, and the failure of spermatogenic cell development is evident from the spermatid level.
    No preview · Article · Nov 2013 · International Urology and Nephrology
  • Yu-Xia Wang · Wei-Jie Zhu · Huan Jiang
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    ABSTRACT: Purpose: Intercourse during menses is a sexual behavior practiced by 3-30 % of sexually active women. Defense responses of mucosal immunity in the female genital tract regulated by sex hormones would be impaired during menses. The present study seeks to clarify whether unprotected sexual activity during menses will increase the risk for antisperm antibody (ASA) production in female. Methods: In this prospective study, 27 women who had a usual practice of vaginal intercourse during menses admitted in the First Affiliated Hospital of Jinan University with infertility were included, while 30 age-matched infertile women without this practice were set as controls. Indirect immunobead test (I-IBT) performed according to the World Health Organization (WHO) manual was used to measure the ASA levels. Results: No case was revealed to develop significant ASA level. 6 of 27 women (22.2 %) in the case group and 1 of 30 women (3.3 %) in the control group were detected to be ASA subpositive, and a significant difference was found in the subpositive incidence between these two groups (P < 0.05). Among these 6 subpositive cases, 3 became pregnant in the subsequent 2-year follow-up after condom therapy for 6 months. Conclusions: Sperm exposure during menses is a risk factor for ASA production in female. Although a precisely causal linkage between ASA and infertility in these women cannot be drawn from the present data, the potential disadvantages of sexual activity during menses should still be given importance.
    No preview · Article · May 2013 · Archives of Gynecology
  • Huan Jiang · Wei-Jie Zhu
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    ABSTRACT: Introduction: Infertility in adulthood is a well-recognized consequence of cryptorchidism, even after successful orchidopexy. Autoimmune reactions against spermatozoa are associated with infertility and often found in cryptorchids. The purposes of this study were to evaluate the linkage between antisperm antibody (ASA) and cryptorchidism, and furthermore, to clarify whether ASA is involved in cryptorchidism-associated infertility. Materials and methods: We investigated a total of 48 infertile males with a history of unilateral (n = 30) or bilateral (n = 18) cryptorchidism who had undergone successful orchidopexy in prepuberty, and 20 age-matched fertile and healthy males were collected as controls. ASA in sperm samples was detected by the direct immunobead test, and semen analysis was performed concomitantly. Results: No infertile case satisfied the diagnostic criteria of ASA-mediated infertility set forth by the World Health Organization. Decreases in both sperm concentration and motility accompanied by increases in abnormal morphology were seen in infertile cryptorchids when compared with the healthy controls. Conclusions: Testicular heat exposure in prepuberty is not a risk factor for ASA production. It is evident that the mechanisms that underlie cryptorchidism-associated infertility do not involve ASA. Poor sperm characteristics in cryptorchids resulting from thermal damage to the testes seem to be responsible for their infertility, even after successful orchidopexy.
    No preview · Article · Apr 2013 · Urologia Internationalis
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    ABSTRACT: Objective To evaluate the effects of different sera on the growth of human granulosa cells (GCs) cultured in vitro. Methods GCs were obtained from women who underwent follicular aspirates during in vitro fertilization (IVF) program. Five groups were divided according to media supplemented with different types of sera. Group A, 10% heat-inactivated fetal bovine serum (FBS); group B, 10% heat-inactivated newborn bovine serum; group C, 10% non heat-inactivated FBS; group D, 10% human serum; group E, bovine serum albumin. Morphological characteristics and viability of GCs measured by trypan blue exclusion assay were evaluated after 24 h of incubation. Results GCs cultured in group A and group B showed multiform morphology compared with other groups. GCs cultured in group D and group E were present with cytoplasmic atrophy and less pseudopodium. Moreover, group A and group B showed a similar level in the viability of GCs (P>0.05), which displayed no difference between group D and group E as well (P>0.05). Group C had a lower level of viability than group A (P<0.05) but a higher level than group D (P<0.01). Conclusion Heat-inactivated sera can improve the growth of GCs. Different types of sera would have different effects on the growth of GCs cultured in vitro. The pre-culture with different types of sera should be performed to get better efficacy.
    No preview · Article · Sep 2012 · Journal of Reproduction and Contraception
  • Jian-Ying Xu · Wei-Jie Zhu · Huan Jiang
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    ABSTRACT: The objective of this study was to investigate the expression of the von Hippel-Lindau (VHL) gene in tissues of human fallopian tube and tubal pregnancy. Twenty patients undergoing salpingectomy for tubal pregnancy were recruited into the study group. Tissues of tubal pregnancy were separated into both the implantation and non-implantation sites as the implantation group and the non-implantation group, respectively. Samples of ampullary fallopian tube during mid-secretory phase were collected from twenty patients with benign uterine disease as the control group. Immunohistochemistry, real-time reverse transcription polymerase chain reaction, and Western blotting analysis were performed to detect expressions of VHL mRNA and protein. The results showed that VHL immunostaining appeared in the cytoplasm of tubal epithelial cells. Expression of VHL mRNA in the implantation group was higher than that in the non-implantation group or the control group (P < 0.01). Intensity of VHL protein in the implantation group was increased compared with that in the non-implantation group (P < 0.05) or in the control group (P < 0.01). There was no difference on expressions of VHL mRNA and protein between the non-implantation group and the control group (P > 0.05). In conclusion, VHL mRNA and protein are present in human tubal tissues. The VHL gene expression is increased in the implantation site of tubal pregnancy, and locally elevated expression of the VHL gene might be associated with human tubal pregnancy.
    No preview · Article · Jun 2012 · Molecular and Cellular Biochemistry
  • Huan JIANG · Wei-jie ZHU
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    ABSTRACT: Objective To investigate the role of circulating antisperm antibody (ASA) in the pathogenesis of endometriosis-associated infertility. Methods Serum samples were collected from 75 infertile women with minimal or mild endometriosis. Indirect immunobead test reporting on the percentage of motile sperm with adherent immunobeads was used to detect the circulating ASA levels. Results No infertile cases enrolled in the present study developed significant ASA level in the serum samples. That is to say, no one could achieve the diagnosis of ASA-mediated infertility according to the World Health Organization criteria (50% or more of the motile sperm with immunobead binding). There were only 5 cases (6.7%) who had 20%-40% of the motile sperm that were found to have adherent particles of ASA-IgG. All the cases were found to be completely absent of ASA-IgA. Conclusions Endometriosis seems to have little impact on the production of circulating ASA. Clearly, ASA is not the key factor implicated in the pathogenesis of endometriosis-associated infertility. The presence of a low titer of ASA in a small proportion of the infertile women with endometriosis may exist by chance and, at least in part, explain the impaired fecundity in those patients.
    No preview · Article · Jun 2012 · Journal of Reproduction and Contraception
  • Xiao-Yi Yang · Wei-Jie Zhu · Huan Jiang
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    ABSTRACT: Tyrosine kinase receptor erythropoietin-producing hepatocellular receptor A2 (EphA2) and its predominant ligand EphrinA1 have been studied extensively for their roles of mediating cell adhesion in epithelial cells. However, EphA2 signaling in human fallopian tube epithelial cells is poorly understood. In this study, primary cultured fallopian tube epithelial cells were used as a model treated with EphrinA1-Fc or IgG-Fc (control), to explore the role of EphA2 signal and its network involved in the regulation of cell adhesion of tubal epithelia cells. The activation of EphA2 and focal adhesion kinase (FAK) was evaluated by western blotting assay in the cultured fallopian tube epithelia cells, of which the cell adhesion activity was determined by MTT assay. A significantly negative correlation was found between phosphorylated-EphA2 (Pho-EphA2) and phosphorylated-FAK (Pho-FAK) after exposure to EphrinA1-Fc (P = 0.000; r = -0.848). EphrinA1-Fc increased Pho-EphA2 and reduced Pho-FAK in seconds, with the apex level of Pho-EphA2 and the nadir level of Pho-FAK detected at the same time (10 min). Cell adhesion of the cultured cells supplemented with EphrinA1-Fc appeared to be weaker than that of the controls at the later time points of the treatment (from 30 to 120 min) (P < 0.05). Taken together, the EphrinA1 addition directly induces an elevated Pho-EphA2 accompanied by a decreased Pho-FAK in human fallopian tube epithelia cells. Furthermore, activation of EphA2 participates in the regulation of fallopian tube cell adhesion via FAK dephosphorylation.
    No preview · Article · Feb 2012 · Molecular and Cellular Biochemistry
  • Huan Jiang · Wei-Jie Zhu · Jing Li
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    ABSTRACT: To investigate the association between β3 integrin-fibronectin signaling and salpingitis.Methods Thirteen chronically inflamed tubal samples were included in the case group, while 29 normal fallopian tube samples were set as the controls. Immunohistochemistry was used to determine the distribution patterns and expressions of β3 integrin and fibronectin in the fallopian tube samples.Resultsβ3 integrin was presented in the ciliated cells within the whole length of the normal fallopian tube, but no staining was detected in the tubal epithelium of salpingitis. A similar distribution pattern of fibronectin was revealed between the cases and the controls, with exclusive staining in the tubal mucosal matrix and the basement membranes of the tubal epithelium and mucosal vessels, whereas no staining was detected in the tubal epithelial cells. Fibronectin in the samples of salpingitis showed a remarkably stronger-staining than that of the normal controls (P<0.01).Conclusion Salpingitis could reduce β3 integrin expression and raise fibronectin expression in the fallopian tube epithelium, concomitantly changed concentrations of β3 integrin and fibronectin seem to have impacts on the onset of salpingitis.
    No preview · Article · Dec 2011 · Journal of Reproduction and Contraception