Shan Huang

Guangxi Medical University, Yung-ning, Guangxi Zhuangzu Zizhiqu, China

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Publications (13)29.59 Total impact

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    ABSTRACT: Background: The aim of this study was to set a reference interval (RI) for osteocalcin (OC) in a healthy Han male population from the Fangchenggang Area Male Health and Examination Survey (FAMHES) project and study the effects of age, BMI, smoking, and alcohol consumption. Methods: We used data from 2018 Han ethnic males who participated in FAMHES project from September 2009 to December 2009. Serum OC values were measured by electrochemiluminescence immunoassay on COBAS 6000 system E601 (Elecsys module) immunoassay analyzers. Results: OC data does not have a normal distribution or Gaussian pattern (Z = 3.791, p < 0.001). After log-transformation, data took on a normal, Gaussian distribution (Z = 0.968, p = 0.306). The RI of serum OC was 12.49 - 43.94 ng/mL. No difference in OC concentration was noted either between non-smoker or smoker groups (p = 0.629) or non-drinker and drinker groups (p = 0.748). OC levels varied with age (r = -0.371, p < 0.001) and BMI (r = -0.331, p < 0.001), and the age-dependent and BMI-dependent RIs were calculated. Conclusions: The RIs for serum OC exhibit slight differences compared to previously reported reference ranges. Age-dependent and BMI-dependent RIs for serum OC should be implemented in clinical laboratories.
    No preview · Article · Aug 2014 · Clinical laboratory
  • Li Xie · Xiao-Nan Liang · Yan Deng · Jian Wang · Yu He · Tai-Jie Li · Shan Huang · Xue Qin · Shan Li
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    ABSTRACT: Quantitative real-time PCR (QRT-PCR) assays are faster, more precise, and more sensitive quantitative laboratory methods for monitoring serial CMV DNA viral load in patients undergoing organ or hematopoietic stem cell transplantation. Clinical laboratories often face practical concerns about the storage of specimens from these patients to ensure the accuracy and reproducibility of CMV viral load test results. Different studies that have assessed CMV DNA stability have shown mixed results. Therefore, we analysed CMV DNA stability of 30 EDTA plasma samples in samples containing between 300 and 100,000 copies/ml over a 21 day period. The concentration of CMV DNA in all samples stored at 4°C for 21 days did not differ significantly from the baseline viral load (t=0.242, p=0.810), and no trend was evident to indicate continued degradation over a two week period.
    No preview · Article · Jul 2014 · Journal of Virological Methods
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    Shan Li · Cuiju Mo · Shan Huang · Shi Yang · Yu Lu · Qiliu Peng · Jian Wang · Yan Deng · Xue Qin · Yinkun Liu
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    ABSTRACT: Hepatocellular carcinoma (HCC) is a male-predominant cancer. Previous studies have focused on the sex-related disparity in HCC, but the underlying mechanism remains unclear. Here, we aimed to discover characteristic biomarkers for male HCC. Clinical samples were subjected to iTRAQ labeling followed by 2DLC-ESI-MS/MS analysis. Seventy-three differential proteins containing 16 up-regulated and 57 down-regulated proteins were screened out in the male HCC group compared to that in female HCC group. Testis-specific Protein Y-encoded 1(TSPY1) is characteristically present in male HCC and was chosen for further investigation. The data from the functional effects of TSPY1 indicated that over-expression of TSPY1 could potentiate HCC cell proliferation, increase soft agar colonization, induce higher cell invasive ability and correlate with the metastatic potential of the HCC cell lines. In addition, TSPY1 and androgen receptor (AR) were co-expressed simultaneously in HCC cell lines as well as in HCC tissue. TSPY1 up- or down-regulation could lead to a high or low level expression of AR. These results implied that TSPY1 may be included in the regulation of AR expression involved in male HCC and it may act as a novel biomarker for male HCC.
    Full-text · Article · Feb 2014 · PLoS ONE
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    ABSTRACT: The objective of this study was to provide a precise evaluation of whether expression levels of excision repair cross-complementation group 1 (ERCC1) are associated with objective response, overall survival (OS), and median survival in patients with advanced bladder cancer treated with platinum-based chemotherapy. Systematic computerized searches of the electronic databases PubMed, EMBASE, Ovid, ASCO, and CNKI were performed and a meta-analysis was carried out to evaluate the correlation between ERCC1 expression levels and objective response rate, OS, or progression-free survival in patients with advanced bladder cancer receiving platinum-based chemotherapy. References within the articles identified were also searched manually. STATA package version 11.0 was used for the comprehensive quantitative analyses. A total of six studies involving 356 patients, of which ERCC1 expression was high/positive in 138 (38.8%) and low/negative in 218 (61.2%), were included in the meta-analysis. The median age of the patients was 63.7 years. The objective response rate favored patients with ERCC1 low/negative expression after platinum-based chemotherapy, but showed no significant difference [odds ratio 0.86, 95% confidence interval (CI) 0.36-2.06, P=0.734]. The median OS time and the median progression-free survival time were significantly prolonged when ERCC1 low/negative expression was compared with ERCC1 high/positive expression (hazard ratio 0.69, 95% CI 0.54-0.89, P=0.004, and hazard ratio 0.76, 95% CI 0.66-0.89, P=0.000, respectively). In conclusion, low/negative expression of ERCC1 was associated with higher objective response, median progression-free survival, and median OS in patients with advanced bladder cancer treated with platinum-based chemotherapy. ERCC1 may be a suitable marker of prognosis and sensitivity to platinum-based chemotherapy in patients with advanced bladder cancer. Larger studies and further clinical trials are warranted to confirm these findings.
    No preview · Article · Sep 2013 · Anti-cancer drugs
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    ABSTRACT: A fibrinolytic enzyme isolated from marine Bacillus subtilis HQS-3 was purified to electrophoretic homogeneity using ammonium sulfate precipitation, alkaline solution treatment, membrane concentration, dialysis, ion exchange, and gel filtration chromatography. SDS-PAGE and gel filtration chromatography showed that it was a monomeric protein with an apparent molecular weight of 26kDa. The purified enzyme was active at pH 6.0-10.0 with an optimum pH of 8.0. It was stable at temperatures ranging from 25 to 37°C, exhibiting maximum activity between 45 to 50°C. The isoelectric point of the enzyme was 9.0-9.2, which was higher than those of other known fibrinolytic enzymes from Bacillus species. PMSF, EDTA, Cu(2+), Zn(2+), and Co(2+) inhibited the enzyme activity significantly. This enzyme did not cause hemolysis in vitro and preferred direct degradation of fibrin in the following order: α, β, and γ-γ chains. Thus, these results suggest that the marine-derived enzyme is a plasmin-like serine metalloprotease, which is distinct from other fibrinolytic enzymes from genus Bacillus.
    No preview · Article · Sep 2013 · International journal of biological macromolecules
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    ABSTRACT: This study aims to investigate the reference values of serum rheumatoid factor (RF) in coastal residents. We collected 1,762 serum samples from 20 - 69 year-old males who participated in the population-based Fangchenggang Area Male Health and Examination Survey from September 2009 to December 2009. The values of serum RF were measured by immunoturbidimetry. The established normal reference value range of serum RF (< or = 18.90 IU/mL) in healthy adult males who live in coastal areas is lower than the current value that is extensively used (20 IU/mL). Age, ethnicity, and smoking specific reference values were calculated. Differences were found within groups, but no significant difference in drinking was observed. It is suggested that every special region should establish respective normal reference values of serum RF in healthy individuals.
    No preview · Article · Jul 2013 · Clinical laboratory
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    ABSTRACT: NANOG is a major transcription factor essential to the stem cell self-renewal and is associated with tumor malignancy, but the NANOG signaling in cancer metastasis is still elusive. In this study, we determined the expression of NANOG in hepatocellular carcinoma (HCC) and investigated its underlying mechanism in the metastasis of HCC. The expression levels of NANOG were examined in tumor tissues by immunohistochemistry. Functional effect of NANOG was investigated both in vivo and in vitro. Our data shows that high level of NANOG expression correlates with metastasis and low survival rate in HCC. HCC cells overexpressing NANOG are characterized by active epithelial-mesenchymal transition (EMT), and exhibit increased ability of invasion, soft agar colonization, sphere formation and drug resistance, whereas SB-431542, an antagonist of activin receptor-like kinase (ALK) receptors, attenuates EMT and invasion of HCC cells. NANOG activates NODAL and CRIPTO-1 to promote SMAD3 phosphorylation and SNAIL expression. The transcriptional activity of NODAL gene is dependent on two NANOG binding motifs in its promoter region. This study shows a significant correlation between the NANOG expression and the expression of NODAL, P-SMAD3 or SNAIL, and the combination of NANOG and P-SMAD3 is a potential predictor of poor prognosis of HCC. Additionally, cells in the tumor edge area displays higher NANOG expression than cells in the tumor center. These results present novel mechanistic insight into an important role of NANOG in HCC metastasis, and suggest a potential application of NANOG in HCC prognosis and treatment.
    No preview · Article · Mar 2013 · The international journal of biochemistry & cell biology
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    ABSTRACT: The association between methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and hepatocellular carcinoma (HCC) risk was inconsistent and underpowered. To clarify the effects of MTHFR gene polymorphisms on the risk of HCC, a meta-analysis of all available studies relating C677T and/or A1298C polymorphisms of MTHFR gene to the risk of HCC was conducted. The authors searched PubMed, EMBASE, Cochrane Library, Web of Science, and Chinese Biomedical Literature database (CBM) for the period up to July 2012. Data were extracted by two independent authors and pooled odds ratio (OR) with 95% confidence interval (CI) was calculated. Metaregression and subgroup analyses were performed to identify the source of heterogeneity. Finally, 12 studies with 2,351 cases and 4,091 controls were included for C677T polymorphism and 6 studies with 1,333 cases and 1,878 controls were included for A1298C polymorphism. With respect to A1298C polymorphism, significantly decreased HCC risk was found in the overall population (CC vs. AA: OR = 0.660, 95%CI 0.460-0.946, P = 0.024; recessive model: OR = 0.667, 95%CI = 0.470-0.948, P = 0.024). In subgroup analyses, significantly decreased HCC risk was found in Asian population (CC vs. AA: OR = 0.647, 95%CI = 0.435-0.963; P = 0.032) and population-based studies (CC vs. AA: OR = 0.519, 95%CI = 0.327-0.823; P = 0.005). With respect to C677T polymorphism, no significant association with HCC risk was demonstrated in overall and stratified analyses. We concluded that MTHFR A1298C polymorphism may play a protective role in the carcinogenesis of HCC. Further large and well-designed studies are needed to confirm this association.
    Full-text · Article · Feb 2013 · PLoS ONE
  • Shan Li · Shan Huang · Z N Mo · Yong Gao · X B Yang · X J Chen · J M Zhao · Xue Qin
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    ABSTRACT: Introduction: Circulating insulin concentrations provide important information for the evaluation of insulin secretion and insulin resistance. Reference intervals are the most widely applied tool for the interpretation of clinical laboratory results. We carried out an analysis of the data available from the Fangchenggang Area Male Health and Examination Survey in order to derive a reference interval for fasting insulin specific to the Chinese population. Methods: A total of 1,434 fasting serum insulin results were obtained from healthy nondiabetic adult men aged 20-69 years, after taking into consideration the inclusion and exclusion criteria. Serum insulin was measured using electrochemiluminescence immunoassays. Nonparametric statistical methods were used to calculate and analyse the data. Results: The reference interval for fasting serum insulin for Chinese adults was in the range 1.57-16.32 μU/mL (median 5.79 μU/mL). Significant correlations were found between fasting serum insulin and glucose and diastolic blood pressure (p < 0.001). Statistically significant differences were observed in insulin concentration with respect to age and body mass index (BMI; p < 0.001). Younger people had a higher fasting serum insulin concentration. Increased fasting serum insulin was also found to be associated with BMI. Conclusion: We established a reference interval for fasting serum insulin in healthy nondiabetic adult Chinese men that is lower than what was previously suggested. BMI and age (but not smoking, alcohol consumption or physical activity) were found to be important factors associated with fasting serum insulin. Our results will help improve the diagnostic interpretation of investigations for metabolic and cardiovascular disorders in a Chinese population.
    No preview · Article · Dec 2012 · Singapore medical journal
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    ABSTRACT: The Interleukin-1A (IL-1A) -889C/T polymorphism has been reported to be associated with Alzheimer's disease (AD) susceptibility, but the results of these previous studies have been inconsistent. The aim of this study was to explore whether the IL-1A -889C/T polymorphism confers susceptibility to AD. All studies published up to July 2011 on the association between the IL-1A -889C/T polymorphism and AD risk were identified by searching electronic databases PubMed, Embase and Alzgene. The association between the IL-1A -889C/T polymorphism and AD risk was assessed by odds ratios (ORs) together with their 95% confidence intervals (CIs). A total of 32 case-control studies including 7,046 AD cases and 7,534 controls were eventually identified. Overall, positive associations of the IL-1A -889C/T polymorphism with AD risk were found in allele comparison T versus C (OR = 1.019, 95% CI= 1.027-1.198), recessive model TT versus CT + CC (OR = 1.278, 95% CI = 1.073-1.522) and dominant model TT + CT versus CC (OR = 1.102, 95% CI = 1.013-1.200). In subgroup analysis stratified by ethnicity, significant associations were demonstrated in Caucasians but not in Asians. In subgroup analysis according to the age of onset, no significant association was detected. The present meta-analysis suggests that the IL-1A is a candidate gene for AD susceptibility. The IL-1A -889C/T889C/T polymorphism may be a risk factor for AD in Caucasians. Further investigations taking the APOE ε4 status and other confirmed genetic factors and potential gene-gene and gene-environmental interactions into consideration for this polymorphism should be conducted.
    No preview · Article · Jan 2012 · Journal of Neurology
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    ABSTRACT: The aim was to establish reference intervals for serum sex hormones in adult men in the Fangchenggang area of China. Serum samples from 1,191 healthy male subjects, aged 20 - 69 years, were collected from the Fangchenggang Area Male Health and Examination Survey (FAMHES). Total testosterone (T), estrogen (E2), luteinising hormone (LH), follicle-stimulating hormone (FSH), and sex hormone-binding globulin (SHBG) were measured by electrochemiluminescence immunoassay (Elecsys). Free testosterone (FTc) was calculated from the concentrations of T, SHBG, and albumin. The total nonparametric reference intervals for male sex hormones in our study were 12.6 - 37.0 nmol/L for T, 0.24 - 0.69 nmol/L for FTc, 65.4 - 207.7 pmol/L for E2, 2.2 - 11.6 IU/L for LH, 1.8 - 16.5 IU/L for FSH, and 18.2 - 95.1 nmol/L for SHBG. Age had a significant positive association with LH (r = 0.218), FSH (r = 0.427), and SHBG (r = 0.427) and a negative association with FTc (r = -0.383) and E2 (r = -0.098), but no significant association with T after adjustment for BMI. The age-dependent reference intervals were also calculated. Higher T and FTc concentrations were found in men who smoke compared with non-smokers, while no significant differences were found in E2, LH, FSH, and SHBG. When stratified for the number of cigarettes smoked per day, we found that the distributions of serum T levels were significantly higher only in the smokers who smoked 11 or more cigarettes a day than the non-smokers. Unlike T, the distributions of serum FTc levels were significantly higher only in the smokers who smoked less than 11 cigarettes a day, while the FSH levels were significantly lower. In clinical practice, single reference intervals can be used for men aged 20 - 69 years for T and E2 measured with the Elecsys method, but separate age-dependent reference intervals should be used for FTc, LH, FSH, and SHBG. In addition, distinct reference intervals for T should be established for non-smokers or smokers (1 - 10 cigarettes/day) and smokers (> or = 11 cigarettes/day).
    No preview · Article · Jan 2012 · Clinical laboratory
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    ABSTRACT: Interleukin-16 (IL16) as a multifunctional cytokine, plays a key role in inflammatory and autoimmune diseases as well as tumour growth and progression. Recently, genetic polymorphisms of IL16 have been reported to be associated with susceptibility to a range of cancers. This study was undertaken to investigate the IL16 gene polymorphisms and determine whether these genetic factors are related to the occurrence of hepatocellular carcinoma (HCC) in a Chinese population. We analyzed three polymorphisms of the IL16 gene (rs11556218T/G, rs4072111C/T and rs4778889T/C) in 206 patients with HBV-related HCC, 270 chronic hepatitis B patients and 264 healthy controls, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and DNA sequencing technology. IL16 polymorphisms were not associated with risk of HCC when compared with healthy controls. However, IL16 polymorphisms were significantly associated with susceptibility to HBV-related HCC when using chronic hepatitis B patients as controls. The rs11556218T/G TG and GG genotypes were associated with significantly increased risk of HBV-related HCC compared with the TT genotype (OR = 1.96 and OR = 3.33). The data also revealed that subjects with the G allele appeared to have higher susceptibility to HBV-related HCC than those with the T allele (OR = 2.10). Under the dominant model genotype TG+GG appeared to be associated with an increased risk of HBV-related HCC (OR = 2.18). The rs4072111C/T TT genotype was associated with a significantly increased risk of HBV-related HCC compared with the CC genotype (OR = 6.67). Polymorphisms of the IL16 gene were significantly associated with susceptibility to chronic hepatitis B when using healthy subjects as controls. The rs11556218T/G TG and GG genotypes were associated with significantly decreased risk of chronic hepatitis B compared with the TT genotype (OR = 0.49 and OR = 0.29). The data also revealed that subjects with the G allele appeared to have lower susceptibility to chronic hepatitis B than those with the T allele (OR = 0.46). Under the dominant model genotype TG + GG appeared to have lower susceptibility to chronic hepatitis B (OR = 0.44). This study showed that the genotypes and allele of IL16 SNPs were associated with chronic HBV infection and HCC. However, further investigation with a larger sample size and haplotype analysis with other SNPs may be required to validate the genetic effects of the IL16 polymorphisms on chronic HBV infection and HCC.
    No preview · Article · Dec 2011 · Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases
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    ABSTRACT: We calculated central-95-interpercentile reference values for serum ferritin (SF). Serum samples were collected from the Fangchenggang Area Male Health and Examination Survey (FAMHES). After screening, we enrolled a total of 1,223 Chinese Han men between the ages of 20 and 69 years. SF values were measured by electrochemiluminescence immunoassay. The two-sided 95-percentile reference values were calculated using both parametric and nonparametric statistical methods. SF values were distributed log-normally. The central-95-percentile reference values for SF were 88.2-771.7 μg/L (nonparametric) or 104.0-832.2 μg/L (parametric). Neither the non-smoking and smoking group (P=0.921) nor the non-drinking and drinking group (P=0.584) exhibited any difference in SF. Higher concentrations of SF were associated with a higher Body Mass Index (BMI) (r=0.266, P<10(-5)) but not with increasing age of the participants (P=0.808). The reference values for SF calculated in this study are higher than previously-reported reference levels.
    No preview · Article · Aug 2011 · Clinical biochemistry

Publication Stats

98 Citations
29.59 Total Impact Points


  • 2011-2014
    • Guangxi Medical University
      Yung-ning, Guangxi Zhuangzu Zizhiqu, China
  • 2013
    • Guangxi University
      • College of Life Science and Technology
      Yung-ning, Guangxi Zhuangzu Zizhiqu, China