Hesheng Luo

Wuhan University, Wu-han-shih, Hubei, China

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Publications (29)51.52 Total impact

  • Ning Cui · Hesheng Luo · Hong Xia · Wei Chen · Guang Yu
    [Show abstract] [Hide abstract] ABSTRACT: Background: The purpose of this study was to study the relationships among Hp infection, secretion of gastrointestinal hormones and contraction of colon muscles of Sprague Dawley rats. Helicobacter pylori (Hp) is proved to be a chief criminal of gastric diseases. It can even be detected outside upper digestive tract like lung and kidney. Scientists found that Hp might modulate hormone secretion by influencing brain-gut axis. However, the specific relationships among Hp infection, hormone secretion and colonic motility are still undefined. Materials and methods: Hp infection procedures were completed by intragastric administration to male Sprague Dawley rats. All model groups' rats were confirmed of Hp infection by rapid urease test and gastric mucosa biopsy. Plasma hormones were determined using Enzyme Immunoassay Kits after Hp infection. Colonic motility was assessed by counting numbers of fecal pellet output and recording contractions of isolated colonic strips through isometric force transducer. Results: Hp infection groups significantly increased fecal pellet output compared with negative Hp infection groups (P < 0.05). The Hp infection groups' mean tension of spontaneous contraction of colon smooth muscle was significantly greater than the negative Hp infection groups' (P < 0.05). Significant differences can be found between Hp infection groups and negative Hp infection groups, concerning the concentration of gastrin, cholecystokinin and substance P (P < 0.05). Conclusions: Hp infection probably activate the regulation of brain-gut peptides and gastrointestinal hormones, which could interact with associated receptors in smooth muscle cells, leading to increased count of fecal pellet output and increased colon motility of smooth muscles.
    No preview · Article · Mar 2016 · The American Journal of the Medical Sciences
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    [Show abstract] [Hide abstract] ABSTRACT: Background/aims: Neurotensin is a gut-brain peptide with both inhibitory and excitatory actions on the colonic musculature; our objective was to understand the implications of this for motor patterns occurring in the intact colon of the rat. Methods: The effects of neurotensin with concentrations ranging from 0.1-100 nM were studied in the intact rat colon in vitro, by investigating spatio-temporal maps created from video recordings of colonic motility before and after neurotensin. Results: Low concentration of neurotensin (0.1-1 nM) inhibited propagating long distance contractions and rhythmic propagating motor complexes; in its place a slow propagating rhythmic segmental motor pattern developed. The neurotensin receptor 1 antagonist SR-48692 prevented the development of the segmental motor pattern. Higher concentrations of neurotensin (10 nM and 100 nM) were capable of restoring long distance contraction activity and inhibiting the segmental activity. The slow propagating segmental contraction showed a rhythmic contraction-relaxation cycle at the slow wave frequency originating from the interstitial cells of Cajal associated with the myenteric plexus pacemaker. High concentrations given without prior additions of low concentrations did not evoke the segmental motor pattern. These actions occurred when neurotensin was given in the bath solution or intraluminally. The segmental motor pattern evoked by neurotensin was inhibited by the neural conduction blocker lidocaine. Conclusions: Neurotensin (0.1-1 nM) inhibits the dominant propulsive motor patterns of the colon and a distinct motor pattern of rhythmic slow propagating segmental contractions develops. This motor pattern has the hallmarks of haustral boundary contractions.
    Preview · Article · Feb 2016 · Journal of neurogastroenterology and motility
  • Pengbo Wu · Yongxiang Shu · Ming Li · Hesheng Luo · Guo Zhang · Shiyun Tan
    [Show abstract] [Hide abstract] ABSTRACT: Objective: To study whether matrix metalloproteinases-9 (MMP) -1562C/T (rs3918242) and MMP-2-1306C/T (rs243865) were associated with the susceptibility on nonalcoholic fatty liver disease (NAFLD) and the interactions between the two factors and central obesity. Methods: Genotypes of 545 patients and 636 subjects with NAFLD under control were examined by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). Unconditional logistic regression (ULR) was performed to assess the NAFLD risk. The gene-environment interactions on the risk of NAFLD were explored by generalized multifactor dimensionality reduction (GMDR) and ULR methods. Results: Results from the case-control analysis indicated that there was an increased risk of developing NAFLD for MMP-9 rs3918242 (TT/CT) genotype carriers, when compared with the non-carriers (CC) , with OR=1.67, 95%CI: 1.32-2.12, P=0.001; Adjusted OR=1.65, 95% CI: 1.31-2.01 (P=0.008). However, risk reduction of NAFLD was found when MMP-2 rs243865 (TT/CT) genotype carriers compared with the non-carriers (CC) , with OR=0.68, 95% CI: 0.53-0.86, P=0.001; with adjusted OR=0.66, 95% CI: 0.49-0.90 (P=0.007). Data from the GMDR showed that gene-environment interaction among rs3918242 and central obesity on the risk of NAFLD might be significant (P=0.001). By using the ULR method, subjects as central obesity-positive but with genotype CT/TT, appeared having 4.50 (95% CI: 2.78-7.17, P= 0.007) times risk of NAFLD, when compared to the central obesity-negative subjects with genotype CC after adjusting for the covariates. Conclusion: MMP-9 rs3918242, MMP-2 rs243865 were associated with risk of NAFLD while both rs3918242 and central obesity showing synergistic effects on the risk of the NAFLD.
    No preview · Article · Feb 2016 · Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
  • [Show abstract] [Hide abstract] ABSTRACT: Background: The mechanism underlying chronic stress-induced gastrointestinal (GI) dysmotility has not been fully elucidated and GI hormones have been indicated playing a role in mediating stress-induced changes in GI motor function. Aims: Our objective was to study the possible role of substance P (SP) in the colonic hypermotility induced by repeated water avoidance stress (WAS) which mimics irritable bowel syndrome. Methods: Male Wistar rats were submitted to WAS or sham WAS (SWAS) (1h/day) for up to 10 consecutive days. Enzyme Immunoassay Kit was used to detect the serum level of SP. The expression of neurokinin-1 receptor (NK1R) was investigated by Immunohistochemistry and Western blotting. The spontaneous contraction of muscle strip was studied in an organ bath system. L-type calcium channel currents (ICa,L) of smooth muscle cells (SMCs) were recorded by whole-cell patch-clamp technique. Results: Fecal pellet expulsion and spontaneous contraction of proximal colon in rats were increased after repeated WAS. The serum level of SP was elevated following WAS. Immunohistochemistry proved the expression of NK1R in mucosa, muscularis and myenteric plexus. Western blotting demonstrated stress-induced up-regulation of NK1R in colon devoid of mucosa and submucosa. Repeated WAS increased the contractile activities of longitudinal muscle and circular muscle strips induced by SP and this effect was reversed by a selective NK1R antagonist. The ICa,L of SMCs in the WAS rats were drastically increased compared to controls after addition of SP. Conclusions: Increased serum SP level and up-regulated NK1R in colon may contribute to stress-induced colonic hypermotility. And L-type calcium channels play a potentially important role in the process of WAS-induced dysmotility.
    No preview · Article · Jan 2016 · Neuropeptides
  • Xiaojing Quan · Hesheng Luo · Hong Xia · Han Fan · Guang Yu · Qincai Tang
    [Show abstract] [Hide abstract] ABSTRACT: Objective: To explore the effects of hydrogen sulfide (H22S) on colonic contraction of rats at low concentration and ion channel mechanisms. Methods: Organ bath recordings were used to examine the contraction of colonic smooth muscle strips. The whole-cell patch-clamp technique was used to record the currents of L-type calcium and large conductance Ca(2+)-activated K(+) (BKCa) channels in smooth muscle cells isolated from 30 male Wistar rats. Results: The H2S donor NaHS ((1-12)×10(-5)mol/L) increased the spontaneous contractions of longitudinal and circular muscle strips in a dose-dependent manner (all P<0.05) and the effect could not be blocked by tetrodotoxin. NaHS (6×10(-5) and 12×10(-5) mol/L) reversibly increased the L-type calcium current (ICa,L) in a dose-dependent manner. And the peak of ICa,L at 0 mV increased from (-3.16±0.47) to (-3.33±0.54) and (-3.65±0.66) pA/pF respectively (n=6, both P<0.05). Current-voltage (I-V) curve had no shift after NaHS treatment and H2S donor caused no change in the curves of steady-state activation. Likewise BKCa channel was significantly inhibited by NaHS (6×10(-5) and 12×10(-5) mol/L) in a dose-dependent manner. And IBKCa at 60 mV decreased from (16.68±1.23) to (15.26±2.67) and (13.80±3.04) pA/pF respectively(both P<0.05). Conclusions: H2S at low concentrations increases the spontaneous contraction of rat colonic smooth muscle in a dose-dependent manner. Such an effect may be due to a direct activation of L-type calcium channel and an inhibition of BKCa channel in smooth muscle cells.
    No preview · Article · Jun 2015 · Zhonghua yi xue za zhi
  • [Show abstract] [Hide abstract] ABSTRACT: Brain-derived neurotrophic factor (BDNF) has prokinetic effects on gut motility and is increased in the colonic mucosa of irritable bowel syndrome. We aimed to investigate the possible involvement of BDNF in stress-induced colonic hypermotility. Male Wistar rats were exposed to daily 1-h water avoidance stress (WAS) or sham WAS for 10 consecutive days. The presence of BDNF and substance P (SP) in the colonic mucosa was determined using enzyme immunoassay kits. Immunohistochemistry and western blotting were performed to assess the expression of BDNF and its receptor, TrkB. The contractions of muscle strips were studied in an organ bath system. Repeated WAS increased the fecal pellet expulsion and spontaneous contractile activities of the colonic muscle strips. Both BDNF and SP in the colonic mucosa were elevated following WAS. Immunohistochemistry revealed the presence of BDNF and TrkB in the mucosa and myenteric plexus. BDNF and TrkB were both up-regulated in colon devoid of mucosa and submucosa from the stressed rats compared with the control. BDNF pretreatment caused an enhancement of the SP-induced contraction of the circular muscle (CM) strips. TrkB antibody significantly inhibited the contraction of the colonic muscle strips and attenuated the excitatory effects of SP on contractions of the CM strips. Repeated WAS increased the contractile activities of the CM strips induced by SP after BDNF pretreatment, and this effect was reversed by TrkB antibody. The colonic hypermotility induced by repeated WAS may be associated with the increased expression of endogenous BDNF and TrkB. BDNF may have potential clinical therapeutic use in modulating gut motility.
    No preview · Article · May 2015 · Digestive Diseases and Sciences
  • Pengbo Wu · Yongxiang Shu · Fang Guo · Hesheng Luo · Guo Zhang · Shiyun Tan
    [Show abstract] [Hide abstract] ABSTRACT: To explore the association between patatin-like phospholipase domain-containing protein 3(PNPLA3) gene rs738409 polymorphism and the susceptibility of non-alcoholic fatty liver disease(NAFLD). Data bases were comprehensively searched to retrace all the related studies on the association between PNPLA3 gene rs738409 polymorphism and susceptibility. Of NAFLD, the pooled OR with 95% CI of the association between PNPLA3 gene rs738409 polymorphism and NAFLD susceptibility were performed using different genetic models. Subgroup analysis based on the source of population and sensitivity analysis was performed to detect the stability of results. 28 original studies with 6 216 patients and 8 218 controls were involved in the final combination of data. Findings from the meta-analyses showed that there were strong associations between PNPLA3 gene rs738409 polymorphism and the susceptibility of NAFLD, under different genetic model comparisons[GG vs. CC:OR = 2.42, 95%CI:1.83-3.21, P < 0.001;CG vs. CC:OR = 1.28, 95%CI:1.15-1.43, P < 0.001;CG+GG vs. CC:OR = 1.31, 95%CI:1.17-1.46, P < 0.001; GG vs. CC+GC:OR = 2.26, 95%CI:1.76-2.90, P < 0.001]. Similar results were found in both Asian and Caucasian populations. Results from the Meta-analysis strongly suggested that there appeared significant association between PNPLA3 gene rs738409 polymorphism and the susceptibility of NAFLD.
    No preview · Article · Apr 2015 · Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
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    Xiaojing Quan · Hesheng Luo · Yin Liu · Hong Xia · Wei Chen · Qincai Tang
    [Show abstract] [Hide abstract] ABSTRACT: Objective: To examine the hypothesis that hydrogen sulfide (H2S) regulates the colonic motility by modulating both L-type voltage-dependent calcium channels and large conductance Ca2+-activated K+ (BKCa) channels. Methods: Immunohistochemistry was performed on rat colonic samples to investigate the localization of the H2S-producing enzymes cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE). The contractions of proximal colonic smooth muscle were studied in an organ bath system. The whole-cell patch-clamp technique was used to record both L-type calcium currents (ICa,L) and BKCa currents in colonic smooth muscle cells (SMCs) isolated from male Wistar rats. Results: Immunohistochemistry revealed the presence of CBS and CSE in mucosa, smooth muscle cells and myenteric neurons. The H2S donor NaHS inhibited spontaneous contractions of the longitudinal muscle and circular muscle strips in a dose-dependent manner, and the inhibitory effects were not blocked by tetrodotoxin. NaHS inhibited the peak ICa,L in colonic SMCs at a membrane potential of 0 mV. The current-voltage (I-V) relationship of L-type calcium channels was modified by NaHS, and the peak of the I-V curve was shifted to the right. NaHS (200 μΜ) evoked a significant rightward shift of the steady-state activation curve and inhibited the inactivation of L-type calcium channels. Furthermore, NaHS reversibly decreased the peak ICa,L in a dose-dependent manner. Likewise, BKCa channels were significantly inhibited by NaHS, and the addition of NaHS caused a time- and dose-dependent reduction in the BKCa current. Conclusion: The relaxant effect of H2S on colonic muscle strips may be associated with the direct inhibition of H2S on L-type calcium channels. H2S may be involved in the regulation of calcium homeostasis in colonic SMCs of rat colon.
    Preview · Article · Mar 2015 · PLoS ONE
  • No preview · Article · Oct 2014 · Genes Chromosomes and Cancer
  • Ning Cui · Hesheng Luo
    [Show abstract] [Hide abstract] ABSTRACT: Objective: To explore the correlative factors and clinical characteristics of digestive system injury during the treatment of anticoagulant and (or) antiplatelet-agents. Methods: A total of 1 443 hospitalized patients on anticoagulant and (or) antiplatelet-agents from January 2010 to December 2013 at Renmin Hospital of Wuhan University were analyzed retrospectively. Results: Their length of hospital stay was from 5 to 27 days. Most of them were elderly males (n = 880, 61.0%) with an average age of (62 ± 6) years. 1 138 patients (78.9%) were farmers, workers or someone without a specific occupation. During the treatment of anticoagulant/antiplatelet-agents, statistical difference existed (P = 0.01) between positively and negatively previous digestive disease groups for actively newly occurring digestive system injury (16.0% (41/256) vs 15.9% (189/1 187)). After the dosing of anticoagulant and (or) antiplatelet-agents, 57 (66.3%, 57/86) patients were complicated by hemorrhage of digestive tract, taking 62.9% (61/97) of all positive result patients for Helicobacter pylori test. Comparing preventive PPI group with no PPI group, there was no marked statistical differences (P = 2.67) for digestive system complication (including hemorrhage of digestive tract) while receiving anticoagulant and (or) antiplatelet-agents (13.9% (74/533) vs 17.1% (156/910)). During anticoagulant and/or antiplatelet-agent therapy, 185 patients (12.8%) were complicated by peptic ulcer or peptic ulcer with bleeding, 40 patients (2.8%) had erosive gastritis and 5 (0.3%) developed acute gastric mucosal lesions. And 42 of 76 patients complicated by hemorrhage of digestive tract underwent endoscopic hemostasis while 2 patients were operated. Ninety-seven patients (6.7%) died, including 61 (62.9%, 61/97) from hemorrhage of digestive tract. The remainder became cured, improved and discharged. Moreover, no significant statistical differences existed (P = 2.29) among three combination group (aspirin, clopidogrel, warfarin), two combination group (aspirin, clopidogrel), exclusive aspirin group and exclusive warfarin group in short-term (<27 d) mortality. Conclusions: It is necessary to clearly dictate the details of medication to the patients not highly educated. Elder, male, history of digestive system disease and Helicobacter pylori infection are possibly highly risk correlative factors for digestive system complications during anticoagulant/antiplatelet-agent therapy. The short-term protective effect of routine dose of PPI is inconspicuous. No significant correlation exists between short-term mortality and the dosage (or type) of anticoagulant/antiplatelet-agents.
    No preview · Article · May 2014 · Zhonghua yi xue za zhi
  • [Show abstract] [Hide abstract] ABSTRACT: MicroRNA-126 (miR-126) has been reported to be a tumor suppressor that targets CXCR4 in colorectal cancer (CRC) cells. This study investigated whether miR-126 has any prognostic impact in patients with CRC. MiR-126 and CXCR4 mRNA expression in 92 pairs of CRC and adjacent nontumorous tissues was examined using quantitative real-time PCR, and CXCR4 protein expression was assessed by immunohistochemistry (IHC) and Western blotting. The correlation between miR-126 and CXCR4 protein expression and clinicopathological features and overall survival rate was determined. MiR-126 was downregulated in CRC tissues that expressed high levels of CXCR4 mRNA. IHC and Western blotting detected high expression of CXCR4 protein in CRC tissues. An inverse correlation was observed between miR-126 and CXCR4 protein expression in CRC tissues. Moreover, low miR-126 and high CXCR4 protein expression was associated with distant metastasis, clinical TNM stage, and poor survival. Multivariate analysis indicated that miR-126 was an independent prognostic factor for overall survival, suggesting its clinical significance as a prognostic predictor in CRC patients. © 2014 Wiley Periodicals, Inc.
    No preview · Article · Apr 2014 · Genes Chromosomes and Cancer
  • [Show abstract] [Hide abstract] ABSTRACT: Gemcitabine (GEM) is a first line chemotherapeutic drug for advanced pancreatic cancer. Dendritic cell (DC) vaccine is a promising method of immunotherapy for malignant tumor. Recent research has indicated that gemcitabine can enhance the efficacy of DC vaccine, but precise mechanism is still unknown. Here, we aimed to investigate the effect of GEM on DCs. The results showed that GEM-treated pancreatic cancer cell medium stimulated maturation of DCs. When co-cultured with autologous T lymphocytes, the pulsed DCs promoted the proliferation of T cells, and exhibited specific cytotoxic T lymphocytes (CTLs) antitumor activity. Further research showed that stimulation of DC maturation may be related to the elevated level of Hsp70 induced by GEM. Our study indicates that GEM changes the immunogenicity of tumor cells, and enhances the efficacy of DC based immunotherapy for pancreatic cancer.
    No preview · Article · Jan 2014 · International immunopharmacology
  • [Show abstract] [Hide abstract] ABSTRACT: Recent evidence shows that altered microRNA-126 (miR-126) expression is implicated in the progression of colorectal cancer (CRC). However, the precise roles and mechanisms of miR-126 in CRC remain unclear. The aim of this study was to investigate the roles of miR-126 in CRC cells and to elucidate miR-126-mediated mechanisms in CRC cells. First, miR-126 expression was analyzed using qRT-PCR in 4 human CRC cell lines (SW480, SW620, HT-29 and HCT-116). Furthermore, the biological properties of miR-126 in CRC cells in vitro were examined by applying Cell Counting Kit 8, cell cycle, cell apoptosis and transwell assays. The mechanisms and pathways of miR-126-mediated in CRC cells were detected by using qRT-PCR, western blotting and luciferase reporter assay. We found that miR-126 overexpression inhibited cell proliferation, migration and invasion, and induced cell arrest in the G0/G1 phase of CRC cells, suggesting that miR-126 functions as a tumor suppressor in CRC cells. Furthermore, we identified the CXC chemokine receptor 4 (CXCR4) as a target of miR-126, and showed that it was negatively regulated by miR-126. We demonstrated that miR-126-mediated tumor suppression might be partly dependent on AKT and ERK1/2 signaling pathways. In conclusion, our data revealed that miR-126 functions as a tumor suppressor in CRC cells by regulating CXCR4 expression via the AKT and ERK1/2 signaling pathways and might be a novel target for therapeutic strategies in CRC.
    No preview · Article · Nov 2013 · International Journal of Oncology
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    Chuncui Ye · Shiyun Tan · Lin Jiang · Ming Li · Peng Sun · Lei Shen · Hesheng Luo
    [Show abstract] [Hide abstract] ABSTRACT: The aim of this study was to determine the clinical and endoscopic manifestations, and pathological characteristics of intestinal schistosomiasis in China, in order to raise awareness of intestinal schistosomiasis and prevent misdiagnosis and missed diagnosis. The retrospective analysis of clinical and endoscopic manifestations, and histopathological characteristics, were conducted for 96 patients with intestinal schistosomiasis. Among these patients, 21 lived in areas that were not infected with Schistosoma and 25 (26%) had no history of schistosome infection or contact with infected water. These patients were mainly hospitalized due to symptoms of diarrhea, mucus and bloody purulent stool. Sixteen cases were of the acute enteritis type, and colonoscopy results determined hyperaemic edema and dispersed small mucosal ulcers. The acute infection in patients was pathologically characterized by the deposition of intact ova with a large quantity of eosinocyte infiltration. Forty‑one cases were of the chronic enteritis type which predominantly manifested with yellow nodules and disorder of the vascular surfaces in the intestines. Thirty‑nine cases were diagnosed with mixed type enteritis, which demonstrated acute and chronic histopathological appearances. In addition, six cases of complicated colorectal cancer were observed. Of the 24 misdiagnosed patients, eight were misdiagnosed with ulcerative colitis, five with colorectal cancer, five with colorectal tuberculosis, four with chronic bacillary dysentery and two with irritable bowel syndrome. Intestinal schistosomiasis demonstrated no specific clinical or endoscopic manifestations and it was determined that patients with abdominal pain, diarrhea and mucous stool may be infected with intestinal schistosomiasis. Epidemiological investigations and colonoscopy combined with multi‑block and multi‑site biopsies may improve the diagnosis of intestinal schistosomiasis. In addition, it is necessary for intestinal schistosomiasis to be followed up by colonoscopy, due to its possible correlation with colorectal tumors.
    Preview · Article · Aug 2013 · Molecular Medicine Reports
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    Dataset: Video S3
    [Show abstract] [Hide abstract] ABSTRACT: The tandem contraction. One tandem contraction is shown. See boxed area in figure 1c. The colon section in the movie was 7.5 cm long, not visible are the proximal 1.5 cm and the distal 1.5 cm of the colon. The video shows in real time. (MOV)
    Full-text · Dataset · Apr 2013
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    Dataset: Video S2
    [Show abstract] [Hide abstract] ABSTRACT: The interrupted LDC. One interrupted LDC is shown. See boxed area in figure 1b. The colon section in the movie was 7.5 cm long, not visible are the proximal 1.5 cm and the distal 1.5 cm of the colon. The video shows in real time. (MOV)
    Full-text · Dataset · Apr 2013
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    Dataset: Video S1
    [Show abstract] [Hide abstract] ABSTRACT: Induced and spontaneous LDCs. Two LDCs are shown, the first one occurred in response to liquid infusion, the second one occured spontaneously. See boxed area in figure 1a. The colon section in the movie was 7.5 cm long, not visible are the proximal 1.5 cm and the distal 1.5 cm of the colon. The video shows in real time. (MOV)
    Full-text · Dataset · Apr 2013
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    Dataset: Video S4
    [Show abstract] [Hide abstract] ABSTRACT: Rhythmic propulsive motor complexes (RPMCs). RPMCs are shown followed by a spontaneous LDC. See boxed area in Figure 1d The colon visible in the movie is 9.1 cm long. Not visible are 1.3 cm at the proximal end and 1.4 cm at the distal end that were used for anchoring colon on in and outflow tubes. The video shows in real time. (MOV)
    Full-text · Dataset · Apr 2013
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    [Show abstract] [Hide abstract] ABSTRACT: To investigate the potential role of hydrogen sulphide (H(2)S) and ATP-sensitive potassium (K(ATP)) channels in chronic stress-induced colonic hypermotility. Male Wistar rats were submitted daily to 1 h of water avoidance stress (WAS) or sham WAS (SWAS) for 10 consecutive days. Organ bath recordings, H(2)S production, immunohistochemistry and western blotting were performed on rat colonic samples to investigate the role of endogenous H(2)S in repeated WAS-induced hypermotility. Organ bath recordings and western blotting were used to detect the role of K(ATP) channels in repeated WAS. Repeated WAS increased the number of fecal pellets per hour and the area under the curve of the spontaneous contractions of colonic strips, and decreased the endogenous production of H(2)S and the expression of H(2)S-producing enzymes in the colon devoid of mucosa and submucosa. Inhibitors of H(2)S-producing enzymes increased the contractile activity of colonic strips in the SWAS rats. NaHS concentration-dependently inhibited the spontaneous contractions of the strips and the NaHS IC(50) for the WAS rats was significantly lower than that for the SWAS rats. The inhibitory effect of NaHS was significantly reduced by glybenclamide. Repeated WAS treatment resulted in up-regulation of Kir6.1 and SUR2B of K(ATP) channels in the colon devoid of mucosa and submucosa. The colonic hypermotility induced by repeated WAS may be associated with the decreased production of endogenous H(2)S. The increased expression of the subunits of K(ATP) channels in colonic smooth muscle cells may be a defensive response to repeated WAS. H(2)S donor may have potential clinical utility in treating chronic stress- induced colonic hypermotility.
    Preview · Article · Feb 2013 · PLoS ONE
  • [Show abstract] [Hide abstract] ABSTRACT: EUS elastography is a novel technique that can be used for distinguishing benign from malignant lymph nodes and focal pancreatic masses. However, the studies pertaining to EUS elastography for differential diagnosis of solid pancreatic masses have reported widely varied sensitivities and specificities. A meta-analysis of all relevant articles was performed to estimate the overall diagnostic accuracy of EUS elastography for differentiating benign and malignant solid pancreatic masses. The literatures were identified by searching in PubMed and Embase databases. Two reviewers independently extracted the information from the literatures for constructing 2 × 2 table. A random-effect model or a fixed-effect model was used to estimate the sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio. A summary receiver operating characteristic curve (SROC) also was constructed. Meta-regression and subgroup analysis were used to explore the sources of heterogeneity. 13 studies including a total of 1042 patients with solid pancreatic masses were selected for meta-analysis. The pooled sensitivity and specificity of EUS elastography for differentiating benign and malignant solid pancreatic masses were 95% (95% confidence interval [CI], 93%-96%), 69% (95% CI, 63%-75%), respectively. The area under SROC (AUC) was 0.8695. Two significant variables were associated with heterogeneity: color pattern and blinding. As a less invasive modality, EUS elastography is a promising method for differentiating benign and malignant solid pancreatic masses with a high sensitivity, and it can prove to be a valuable supplement to EUS-FNA.
    No preview · Article · Sep 2012 · Pancreatology