Rui Hai Liu

Cornell University, Итак, New York, United States

Are you Rui Hai Liu?

Claim your profile

Publications (104)295.91 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Tremella fuciformis Berk, a popular nutritious mushroom in China, has attracted increasing attention due to its various bioactivities. In the present work, T. fuciformis polysaccharides (TP) were extracted with hot water. It mainly consisted of mannose, as well as 10.77% (w/w) uronic acid. In additional, the anti-photoaging effects of TP were evaluated using a 30-day UV-irradiated animal assay. TP could efficiently reduce the water and collagen losses of the skin, and inhibit the increase of glycosaminoglycans. Moreover, a histopathological study showed that, UV-induced skin structural alterations were alleviated as well as repairing endogenous collagen breakdown and maintaining the ratio of type I/III collagen, after oral treatment of TP. The activities of SOD, GSH-Px and CAT were increased compared to the irradiated control group without treatment. Accordingly, TP can be used as a potential functional food supplement for skin function protection.
    No preview · Article · Jan 2016
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Free and bound phenolic compounds were extracted from four varieties (BQ, Dulihuang; CHQK, Changheiqingke; ZQ25, Zangqing 25 and ZQ320, Zangqing 320) of dehulled highland barley. Phenolics composition, antioxidant and antiproliferative activities were analysed. Total phenolics ranged from 333.9 ± 17.1 to 460.8 ± 5.7 mg gallic acid equivalents (GAE)/100 g grain (dry weight, DW), total flavonoids are between 145.5 ± 10.0 and 247.4 ± 20.3 mg catechin equivalents (CE)/100 g grain (DW), and ferulic acid is the dominant phenolic compound. These extracts showed excellent antioxidant activities determined by oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays, as well as potent antiproliferative activity towards HepG2 human liver cancer cells. Bound phenolics make a significant contribution to total phenolics content, antioxidant and anticancer activities. This study suggested that highland barley exhibits the unique phenolic compound profile with potential health benefits, and it may serve as ingredients for functional foods.
    Full-text · Article · Dec 2015
  • [Show abstract] [Hide abstract]
    ABSTRACT: The present study aimed to characterize and investigate the polysaccharide fractions of mulberry fruit, and their antioxidant and hypoglycemic activities. Mulberry fruit polysaccharides (MFP) were extracted by using hot water as the solvent and fractioned by using a DEAE-Sepharose fast-flow column. In total four eluents of water (MFP-1), 0.1 M NaCl (MFP-2), 0.2 M NaCl (MFP-3) and 0.3 M NaCl (MFP-4) were fractionated. Arabinose, galactose, glucose, xylose and mannose were the main components present in MFP-1, while MFP-2 and MFP-3 were composed of arabinose and galactose, and MFP-4 of arabinose, galactose and glucose. High-performance gel permeation chromatography (HP-GPC) analysis indicated a narrower molecular weight distribution in the MFP-1 fraction. Scanning electron microscopy (SEM) exhibited a smooth surface for MFP-1 and MFP-3 fractions, whereas MFP-2 and MFP-4 have pore openings and flocculent fibers on a smooth surface. Tertiary structure analyses indicated that none of the fractions had a triple-helical conformation. On the whole, MFP-3 and MFP-4 showed better antioxidant activity and inhibitory effects on α-amylase and α-glucosidase compared to MFP-1 and MFP-2. These results show significant potential for the antioxidant and hypoglycemic activities of MFP-3 and MFP-4 indicating the need for their further exploration as potential antidiabetic agents.
    No preview · Article · Nov 2015
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: It is evident from various epidemiological studies that consumption of fruits and vegetables is essential to maintain health and in the disease prevention. Present study was designed to examine phenolic contents and antioxidant properties of three varieties of Crataegus pinnatifida (Chinese hawthorn). Shanlihong variety exhibited elevated levels of total phenolics and flavonoid contents, including free and bond phenolics. Procyanidin B2 was most abundant phenolic compound in all samples, followed by epicatechin, chlorogenic acid, hyperoside, and isoquercitrin. The free ORAC values, and free hydro-PSC values were 398.3-555.8μmolTE/gDW, and 299.1-370.9μmolVCE/gDW, respectively. Moreover, the free cellular antioxidant activity (CAA) values were 678-1200μmol of QE/100g DW in the no PBS wash protocol, and 345.9-532.9μmol of QE/100g DW in the PBS wash protocol. C. pinnatifida fruit could be valuable to promote consumer health. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Full-text · Article · Nov 2015 · Food Chemistry
  • Source
    Dataset: LC-多糖3
    Chao Li · Lijun You · Xiong Fu · Qiang Huang · Shu-Juan Yu · Rui Hai Liu

    Full-text · Dataset · Oct 2015
  • Source

    Full-text · Dataset · Oct 2015
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Flaxseed (Linum usitatissimum L.) is well known for its health benefits and chemical composition. This study aimed to evaluate the dynamic changes caused by germination in vitamin C, phenolic compounds and antioxidant activities of flaxseed. It was observed that eight-day germination of flaxseed sprouts caused increases in phytochemical contents, that is 22.1-fold increase in vitamin C, 2.67-fold increase in phenolics and 5.48-fold increase in flavonoid contents as compare to initial concentration in flaxseeds on dry weight basis. The stimulation was also noted in the three identified phenolic acid ingredients including caffeic acid, p-coumaric acid and ferulic acid. Similarly, the total PSC and ORAC values of flax sprouts at day 8 increased by 3- to 4-folds compared to nongerminated flaxseeds. It is revealed that comparatively, 8-day germination of flax sprouts leads to a significant increase in vitamin C, phenolic constituents and total antioxidant activities than those of flaxseeds.
    Full-text · Article · Aug 2015 · International Journal of Food Science & Technology
  • Linzhen Wang · Hong Wang · Qinrun Lai · Tong Li · Xiong Fu · Xinbo Guo · Rui Hai Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: The objective of this investigation was to study the dynamic changes of ascorbic acid, tocopherols and their antioxidant activity of soya bean sprouts during germination. Results showed that the expression levels of genes related to biosynthesis of ascorbic acid and tocopherols dramatically increased during germination. The expression levels of most genes were up to the peak at day 3 and kept constantly until the end of germination. The contents of ascorbic acid, α-tocopherol, γ-tocopherol, δ-tocopherol and total tocopherols increased during soya bean sprouts germination and reached peak levels on day 5, of 74.42 ± 1.64 mg/100 g DW, 7.64 ± 0.13 mg/100 g DW, 15.84 ± 1.85 mg/100 g DW, 12.37 ± 1.06 mg/100 g DW, 35.85 ± 2.81 mg/100 g DW, respectively. The antioxidant activity of soya bean sprouts increased during germination and reached peak level on day 5 to 311.01 ± 49.01 μmol ASA equiv./100 g DW. Therefore, germination significantly increased the biosynthesis of ascorbic acid, tocopherols and antioxidant activity of soya bean sprouts.
    No preview · Article · Jul 2015 · International Journal of Food Science & Technology
  • [Show abstract] [Hide abstract]
    ABSTRACT: The present study evaluated whether acute consumption of freeze concentrated yerba mate (Ilex paraguariensis A. St. Hil.) infusion (CM) promotes improvement on the antioxidant and lipid peroxidation parameters in healthy individuals when compared to the traditional mate infusion (TM). In this cross-over study, 31 fasted volunteers ingested 200mL of TM, CM, or water (control). Blood samples were withdrawn before and 1h after beverages intake for the measurement of the antioxidant enzyme activities, catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), reduced glutathione (GSH), lipid peroxidation (LOOH), and antioxidant capacity of serum (FRAP assay). CM intake promoted significant enhancement of the CAT (28.7%), SOD (21.3%), and GPx (9.6%) activities, and increased the levels of GSH (8.8%) and serum antioxidant capacity (7.5%), while TM increased only GSH values (8.3%). Serum levels of LOOH did not change significantly after consumption of both mate infusions. Acute consumption of CM by healthy individuals improved the blood antioxidant defense systems more efficiently than the traditional yerba mate and did not promote pro-oxidant effects.
    No preview · Article · Jul 2015 · Lebensmittel-Wissenschaft und-Technologie
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Mango (Mangifera indica L.), also called "the king of fruits", is one of the most popular fruits in tropical regions. Pulp and peel samples of mango cultivars were analyzed to estimate total phenolic, total flavonoid and total anthocyanin contents. Phenolic acids, hydrophilic peroxyl radical scavenging capacity (hydro-PSC) and oxygen radical scavenging capacity (ORAC) in vitro were also determined. Total phenolics and flavonoid contents were found maximum in the peel of Xiao Tainang and Da Tainang cultivars, respectively, whereas Xiao Tainang also exhibited significant antioxidant capacity. Noteworthy, concentrations of gallic acid, protocatechuic acid, ferulic acid, chlorogenic acid and caffeic acids at 79.15, 64.33, 33.75, 27.19 and 13.62 mg/100 g fresh weight (FW) were quantified for Da Tainang, Xiao Tainang and of Jidan cultivars, respectively. Comparatively, a higher level of phenolics and significant antioxidant capacity in mango peel indicated that it might be useful as a functional food and value-added ingredient to promote human health.
    Full-text · Article · Jun 2015 · International Journal of Molecular Sciences
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to evaluate the influence of the in vitro gastrointestinal digestion of yerba mate extract on the cellular antioxidant activity (CAA) and cytotoxic and antiproliferative action toward HepG2 cells. The total reducing substances, isolated phenolic acids, total flavonoids and total antioxidant activity of the yerba mate extract decreased significantly after in vitro digestion. The digested extract promoted lower CAA and inhibition of HepG2 cell proliferation when compared to the non-digested extract (P<. 0.05). No significant difference was observed in the cytotoxicity assay. Dose-response correlations were found for both yerba mate extracts for CAA, antiproliferative activity and cytotoxicity. The simulated gastrointestinal digestion of yerba mate extract promoted a significant decrease in the phytochemical content, total antioxidant activity, CAA and inhibition of HepG2 cell proliferation. Nevertheless, digested yerba mate extract still has considerable CAA and antiproliferative activity, which merits further investigation in in vivo studies.
    No preview · Article · May 2015 · Food Research International
  • [Show abstract] [Hide abstract]
    ABSTRACT: Single-factor experiment and Box-Behnken design (BBD) were applied to optimize the ultrasound-assisted extraction of mulberry fruits polysaccharides (MFP). Under optimum conditions: ratio of water to raw material 40.25, extraction temperature 69°C, ultrasonic power 190W and extraction time 75min, the MFP yield was 3.13% (±0.07%), in accordance to the predicted value of 3.04%. The mulberry fruits polysaccharides fractions was obtained by deproteinization (MFP-1), followed by decolorization and deionization (MFP-2). Carbohydrate content in MFP, MFP-1 and MFP-2 was 58.61% (±1.47%), 69.98% (±0.91%), 81.18% (±1.29%), as well as proteins was estimated 16.50% (±0.86%), 1.57% (±0.63%), 1.02% (±0.18%), respectively. The FT-IR indicated that MFP, MFP-1 and MFP-2 were acidic polysaccharides. The MFP-1 exhibited the strongest antioxidant activity, while MFP-2 showed the strongest hyperglycemic activity in vitro. This may be caused by their different compositions and physical properties in the different mulberry fruit polysaccharides fractions. Copyright © 2015 Elsevier Ltd. All rights reserved.
    No preview · Article · May 2015 · Carbohydrate Polymers
  • Li Zhen Zhang · Rui Hai Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: Commonly consumed foxtail millet varieties Jingu28 and Jingu34 were compared in terms of phytochemical composition, antioxidant property, and antiproliferative activity. The cellular antioxidant activity (CAA) was evaluated based on HepG2 cell cultivation. Antiproliferative properties against HepG2 and MDA cell were assayed by methylene blue assay. Total phenolic content (TPC) was 78.79 and 114.22mg gallic acid equiv/100g DW in Jingu28 and Jingu34. Both varieties contained ferulic acid, chlorogenic acid, caffeic acid and p-coumaric acid, syringic acid. Xanthophylls and zeaxanthin were also detected. Peroxyl radical scavenging capacity of the foxtail millet were 228.13 (Jingu28) and 355.03 (Jingu34) μmol of vitamin C equiv/100g, respectively. CAA values of the foxtail millet varieties ranged from 1.52 to 8.97μmol quercetin equiv/100g DW. The proliferation of MDA and HepG2 cancer cells were significantly inhibited in a dose-dependent manner after exposure to Jingu28 and Jingu34 extractions. Copyright © 2014. Published by Elsevier Ltd.
    No preview · Article · May 2015 · Food Chemistry
  • [Show abstract] [Hide abstract]
    ABSTRACT: Beneficial health effects of polyphenols depend on their daily consumption which may reflect the dietary pattern of a population. In this way, the objective of this study was to evaluate the in vitro and cellular antioxidant activity of Feijoada whole meal compared to its individual ingredients. Kale was the ingredient with the highest total phenolic content and in vitro antioxidant activity. Black beans and rice were the main sources of bound phenolics, accounting for approximately 50% of the total phenolic content in each one. Black beans were also the main providers of phenolics in the feijoada whole meal. Dose dependent effect was observed for black beans, kale, and orange when cellular antioxidant activity (CAA) was measured, being orange the ingredient with the lowest EC50 value. Antiproliferative activity using HepG2 cells was only observed for the orange and kale extracts at 60 mg/ml. Feijoada whole meal extract and the combination of individual ingredients had no effect in this cancer cell line compared to the control. The interaction among ingredients composing a meal as for whole meals itself should be used more often as an approach to understand the relationship between dietary pattern and health benefits.
    No preview · Article · Apr 2015 · Lebensmittel-Wissenschaft und-Technologie
  • Source
    Chao Li · Lijun You · Xiong Fu · Qiang Huang · Shu-Juan Yu · Rui Hai Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: A new heterpolysaccharide, here called P1, was isolated from the fruit clusters of Prunella vulgaris using hot water extraction method. Chemical and physical analyses indicated that P1 had a spherical conformation with an average molecular weight of 1,750 kDa, and consisted of arabinose (28.37%), xylose (54.67%), mannose (5.61%), glucose (5.46%), and galactose (5.89%). The main linkage types of P1 were proved to be (1→5)-linked α-L-Ara, (1→)-linked α-L-Ara, (1→3)-linked α-D-xyl, (1→3)-linked β-D-Gal, (1→3,6)-linked β-D-Gal, (1→3,6)-linked α-D-Man and (1→6)-linked α-D-Glc according to periodate oxidation-Smith degradation and NMR analyses. P1 could significantly enhance the secretion of NO, TNF-α, and IL-6 in murine RAW 264.7 cells involving the toll-like receptor 2 (TLR2), TLR4 and complement receptor 3 (CR3). Further studies showed that P1 exhibited stable immune activities in the pH range of 4.0-10.0 and below 121 ℃. The results suggested that P1 could be used as a potent immunomodulatory agent in functional foods and pharmacological fields.
    Full-text · Article · Mar 2015
  • Source
    Dan Gan · Xiaoxiong Zeng · Rui Hai Liu · Hong Ye
    [Show abstract] [Hide abstract]
    ABSTRACT: The antiproliferative and apoptosis-inducing activities of an acidic polysaccharides fraction from Pholiota dinghuensis Bi mycelium (PDP-3) against human breast cancer MCF-7 cells were investigated. As a result, PDP-3 displayed a significant prevention of cell growth towards MCF-7 cells, with an EC50 value of 38.35 µg/mL. Furthermore, PDP-3 induced significant apoptosis towards MCF-7 cells. From Western blot results, up-regulated p21 and down-regulated cyclin D1, CDK4 and PCNA, up-regulated Bax and down-regulated Bcl-2, caspase-9 and caspase-3 were found in the protein expressions of MCF-7 cells. In MCF-7 cells treated with PDP-3, down-regulated TRAF2 and up-regulated ASK1, phosphorylated of p38 and p53 were also found. Notably, PDP-3 treatment represented a significant decrease in both nuclear and cytoplasmic expression of ERα in MCF-7 cells. Therefore, p38/MAPK signal transduction pathway was the potential mechanism of PDP-3 in regulating both cell proliferation and apoptosis in oestrogen-dependent human breast cancer MCF-7 cells.
    Preview · Article · Jan 2015 · Journal of Functional Foods
  • Chao Li · Qiang Huang · Xiong Fu · Xiu-Jie Yue · Rui Hai Liu · Li-Jun You
    [Show abstract] [Hide abstract]
    ABSTRACT: Water-soluble polysaccharides from Prunella vulgaris Linn (P. vulgaris) were fractionated using DEAE-Sepharose fast-flow column to obtain several eluents of water (PV-P1), 0.1M NaCl (PV-P2) and 0.2M NaCl (PV-P3). Structural analyses showed that PV-P1 had a higher molecular weight and degree of branching as compared to PV-P2 and PV-P3. Tertiary structure analyses indicated that PV-P1, PV-P2 and PV-P3 did not have triple-helical conformation. PV-P2 and PV-P3 showed stronger antioxidant activities than PV-P1, as measured radical scavenging capacities. PV-P1 showed stronger immunomodulatory activities than PV-P2 and PV-P3 in term of stimulation of the production of pro-inflammatory cytokines, including nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in murine macrophage RAW 264.7 cells. PV-P1, PV-P2 and PV-P3 did not exhibit cytotoxicities against RAW 264.7 at the concentrations tested. These results suggest that P. vulgaris polysaccharides could be explored as potential antioxidant and immunomodulatory agents for the complementary medicine or functional foods. Copyright © 2015. Published by Elsevier B.V.
    No preview · Article · Jan 2015 · International Journal of Biological Macromolecules
  • [Show abstract] [Hide abstract]
    ABSTRACT: Traditional medicines comprise a variety of health practices, approaches, knowledge, and beliefs. Documentation of traditional knowledge, estimation of total phenolics and antioxidant properties of plant species used as wild vegetables and in traditional medicines by the local communities of Lesser Himalayas-Pakistan are targeted. Interviews, questionnaires, and focus group conversation with local informants are carried out to record ethno-medicinal values. Used value, percentage of people who have traditional knowledge, preference ranking and informant consensus factors were also measured. Standard analytical methods were applied to estimate phenolic contents and antioxidant properties in water and acetone extracts. A total of 39 plant species used as culinary vegetable and to treat 44 different health disorders are investigated. Significant levels of use value (0.571) and preference ranking (58% PPK, PR-5) are calculated for Ficus palmata, Ficus carica and Solanum nigrum respectively. Elevated levels of total phenolics (144.5mg GAE/100g FW), and flavonoid contents (142.5mg RtE/100g FW) are deliberated in the water extracts of Origanum vulgare, while Ficus palmata exhibits highest flavonol contents (142.7mg RtE/100g FW). Maximum DPPH activity is noted in the flowering buds of Bauhinia variegata (85.34%). However, highest values for OH(-) radical scavenging activity (75.12%), Fe(+3) reducing antioxidant power (54.50µM GAE/100g, FW), and total antioxidant capacity (180.8µM AAE/100g, FW) are measured in the water extracts of Origanum vulgare. Lesser Himalayas is a rich source of traditional cultural heritage, and plant biodiversity, which are under threat and necessitate urgent documentation. Present study is focused on the plant species used in traditional medicines and culinary vegetables as well. Preliminary determination of phenoloic contents and antioxidant properties of various plant species are carried out. Present work will introduce new resource of medicinal and food plants, which could be used as functional food. Furthermore, phytochemical profiling, cellular based antioxidant properties, and isolation of active ingredients will be useful for consumers, and in the pharmaceutical and nutraceutical industries of the country. Copyright © 2014. Published by Elsevier Ireland Ltd.
    No preview · Article · Jan 2015 · Journal of Ethnopharmacology
  • Source
    Yongsheng Chen · Gu Chen · Xiong Fu · Rui Hai Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: Consumption of plant foods has been negatively associated with risk of developing chronic diseases, which is partly attributed to their rich and diverse phytochemicals. In order to promote the rational and effective application of Adinandra tea (Adinandra Jack), a traditional Chinese tea (Shiyacha) widely consumed as a health beverage, the complete phytochemical profiles and antioxidant activities of four varieties of Adinandra tea were analyzed. They were rich in phenolics and flavonoids, ranging from 71.29 to 140.54 mg gallic acid equivalents /g, and from 19.13 to 88.72 mg catechin equivalents /g respectively. Their antioxidant capacities were high as revealed by ORAC, PSC and CAA assay. Obvious antiproliferation effect was observed in HepG2 and MCF-7 cells, with EC50 ranging from 1.05 to 6.44 mg/mL, and from 2.26 to 8.02 mg/mL respectively. Among the four varieties compared, Nitida and Millettii had higher CAA value and anti-proliferation activity, while Latifolia contained considerable bound phenolics.
    Full-text · Article · Dec 2014 · Journal of Agricultural and Food Chemistry
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to investigate the synergistic antioxidant potential and protective effect of grape seed procyanidins (GSP) in combination with Auricularia auricular-judae polysaccharides (AAP IV) on radiation injury in splenocytes. Rat splenocyte irradiation resulted in significantly higher apoptosis rate, malondialdehyde (MDA) (p < 0.005), reactive oxygen species (ROS) (p < 0.01); cell viability, total superoxide dismutase (T-SOD) (p < 0.01), catalase (CAT) (p < 0.01), glutathione peroxidase (GSH-PX) (p < 0.05), activity and glutathione (GSH) (p < 0.01) levels were significantly reduced, compared with the control group. "GSP + AAP IV" treatment of rat splenocytes at doses of "GSP (0.3 μg/mL) + AAP IV (50 μg/mL)" displayed higher radioprotective and antioxidative effects than the administration of either GSP or AAP IV, as evident by lower levels of MDA (p < 0.001) concentration, as well as higher cell viability and T-SOD (p < 0.05), CAT (p < 0.005), GSH-PX (p < 0.01) and GSH content compared to the radiation group. In addition, in vivo studies have shown that "GSP + AAP IV" significantly ameliorated the decrease of spleen index (p < 0.005) and spleen GSH (p < 0.005) levels and significantly inhibited the increase of MDA (p < 0.005) levels of spleen with radiation-induced damage, compared with the non-treated group. The in vivo and in vitro results suggested that GSP and AAP IV have a synergistic protective effect against radiation-induced injury by improving the antioxidant and immunomodulation activities.
    Preview · Article · Dec 2014 · Molecules