Nancy F. Refulio-Rodriguez

University of Washington Seattle, Seattle, Washington, United States

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Publications (5)7.71 Total impact

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    Nancy F Refulio-Rodriguez · Richard G Olmstead
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    ABSTRACT: The Lamiidae, a clade composed of approximately 15% of all flowering plants, consists of five orders: Boraginales, Gentianales, Garryales, Lamiales, and Solanales; and four families unplaced in an order: Icacinaceae, Metteniusiaceae, Oncothecaceae, and Vahliaceae. Our understanding of the phylogenetic relationships of Lamiidae has improved significantly in recent years, however, relationships among the orders and unplaced families of the clade remain partly unresolved. Here, we present a phylogenetic analysis of the Lamiidae based on an expanded sampling, including all families together, for the first time, in a single phylogenetic analyses. Phylogenetic analyses were conducted using maximum parsimony, maximum likelihood, and Bayesian approaches. Analyses included nine plastid regions (atpB, matK, ndhF, psbBTNH, rbcL, rps4, rps16, trnL-F, and trnV-atpE) and the mitochondrial rps3 region, and 129 samples representing all orders and unplaced families of Lamiidae. Maximum Likelihood (ML) and Bayesian trees provide good support for Boraginales sister to Lamiales, with successive outgroups (Solanales + Vahlia) and Gentianales, together comprising the core Lamiidae. Early branching patterns are less well supported, with Garryales only poorly supported as sister to the above 'core' and a weakly supported clade composed of Icacinaceae, Metteniusaceae, and Oncothecaceae sister to all other Lamiidae. Our phylogeny of Lamiidae reveals increased resolution and support for internal relationships that have remained elusive. Within Lamiales, greater resolution also is obtained, but some family interrelationships remain a challenge.
    Preview · Article · Feb 2014 · American Journal of Botany
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    ABSTRACT: We investigated the phylogeny of the New World grass Dissanthelium to explore its monophyly, to examine relationships within the genus, and to investigate its relationship with Poa. Molecular phylogenetic analyses including a thorough sampling of Dissanthelium (seventeen of the 20 species) and DNA sequences from the nuclear ribosomal ITS and plastid trnT-trnL-trnF regions suggest that Dissanthelium is not monophyletic and is nested within Poa. However, ten species form a strongly supported clade (the Dissanthelium clade) in the ITS tree. We propose treating Dissanthelium and Tovarochloa as taxonomic synonyms of Poa. We erect two new sections in Poa: sect. Dissanthelium, comprising the Dissanthelium clade and D. peruvianum, and the monotypic sect. Tovarochloa. The necessary new combinations (Poa aequalis, P. amplivaginata, P. calycina var. mathewsii, P. gigantea, P. macusaniensis, P. rahuii, and P. trollii) and new names (Poa sections Dissanthelium and Tovarochloa, P. apiculata, P. arcuata, P. boliviana, P. congesta, P. deminuta, P. linearifolia, P. parvifolia, P. serpaiana, P. swallenii, and P. thomasii) are effected herein.
    Full-text · Article · Mar 2012 · Systematic Botany
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    ABSTRACT: Recent analyses employing up to five genes have provided numerous insights into angiosperm phylogeny, but many relationships have remained unresolved or poorly supported. In the hope of improving our understanding of angiosperm phylogeny, we expanded sampling of taxa and genes beyond previous analyses. We conducted two primary analyses based on 640 species representing 330 families. The first included 25260 aligned base pairs (bp) from 17 genes (representing all three plant genomes, i.e., nucleus, plastid, and mitochondrion). The second included 19846 aligned bp from 13 genes (representing only the nucleus and plastid). Many important questions of deep-level relationships in the nonmonocot angiosperms have now been resolved with strong support. Amborellaceae, Nymphaeales, and Austrobaileyales are successive sisters to the remaining angiosperms (Mesangiospermae), which are resolved into Chloranthales + Magnoliidae as sister to Monocotyledoneae + [Ceratophyllaceae + Eudicotyledoneae]. Eudicotyledoneae contains a basal grade subtending Gunneridae. Within Gunneridae, Gunnerales are sister to the remainder (Pentapetalae), which comprises (1) Superrosidae, consisting of Rosidae (including Vitaceae) and Saxifragales; and (2) Superasteridae, comprising Berberidopsidales, Santalales, Caryophyllales, Asteridae, and, based on this study, Dilleniaceae (although other recent analyses disagree with this placement). Within the major subclades of Pentapetalae, most deep-level relationships are resolved with strong support. Our analyses confirm that with large amounts of sequence data, most deep-level relationships within the angiosperms can be resolved. We anticipate that this well-resolved angiosperm tree will be of broad utility for many areas of biology, including physiology, ecology, paleobiology, and genomics.
    Full-text · Article · Apr 2011 · American Journal of Botany
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    Lynn J. Gillespie · Robert J. Soreng · Roger D. Bull · Surrey W.L. Jacobs · Nancy F. Refulio-Rodriguez
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    ABSTRACT: The worldwide temperate subtribe Poinae comprises the largest grass genus, Poa (500+ species), and multiple additional small genera. We explore generic boundaries and relationships among genera of Poinae using nuclear ribosomal internal transcribed spacer data (ITS) and plastid trnT-trnL-trnF (TLF) sequence data. ITS and TLF analyses are mostly congruent with regards to circumscription of genera, and with respect to relationships among Poinae genera, but tree structure is generally better supported among genera in the ITS strict consensus tree. ITS and TLF both support two main Poinae lineages: (i) Poa and (ii) a clade comprising all other sampled Poinae. Nine small genera were nested within the large Poa clade, including Aphanelytrum, Dissanthelium, and Tovarochloa, supporting inclusion of these as sections within Poa. In the second clade, three subclades support close relationships among Nicoraepoa, Hookerochloa, and Arctagrostis; Arctophila and Dupontia; and Apera, Bellardiochloa, and Ventenata. Genera of the related subtribes Alopecurinae, Cinninae, and Miliinae were mixed among or in part external to Poinae in different ways in ITS and TLF analyses, and only subtribe Puccinelliinae was strongly supported and monophyletic. ITS analyses supported placement of Catabrosella, Hyalopoa, and Paracolpodium in Puccinelliinae (no TLF data available). The position of Poa subgenus Arctopoa is incongruent between the two analyses: TLF data support inclusion within Poa and ITS data place it outside of Poa. Similarly, TLF data resolves the genus Aniselytron outside of Poa, whereas ITS data place it within Poa. Based on these results and a consideration of morphology, we recognize Arctopoa and Aniselytron as genera, probably of ancient hybrid origins. Nothogenus ×Duarctopoa is coined for Arctopoa× Dupontia, with a single nothospecies ×Duarctopoa labradorica. A new combination is provided for Poa subg. Sylvestres.La sous-tribu des régions tempérées mondiales des Poinae comporte les Poa, le plus grand genre d'herbes avec plus de 500 espèces, ainsi que de multiples petits genres additionnels. Les auteurs ont exploré les limites génériques et les relations au sein des Poinae, en utilisant les données de l'espaceur interne transcrit de l'ADN ribosomique nucléique (ITS) et les données de séquences plastidiques trnT-trnL-trnF (TFL). Les analyses ITS et TFL concordent généralement quant à la circonscription des genres et aux relations entre les genres de Poinae, mais le dendrogramme des genres est généralement mieux supporté dans l'arbre ITS à strict consensus. Les ITF et les TFL supportent tous les deux, deux lignées principales de Poinae: (i) Poa et (ii) un clade comportant tous les autres échantillons de Poinae. Neuf petits genres se retrouvent au sein du grand clade Poa, incluant Aphanelytrum, Dissanthelium et Tovarochloa, supportant leur regroupement dans une section les Poa. Dans le second clade, trois sous clades supportent d'étroites relations entre Nicoraepoa, Hookerochloa et Arctagrostis; Arctophila et Dupontia; et Apera, Bellardiochloa et Ventenata. Les genres des sous tribus apparentées Alopecurinae, Cinninae et Miliinae se retrouvent en partie mêlés avec ou en marge des Poinae de différentes manières selon les analyses ITS et TFL; et seulement la sous tribu Puccinelliinae trouve un solide support monophylétique. Les analyses ITS supportent la localisation des Catabrosella, Hyalopoa et Paracolpodium dans les Pucinelliinae (aucun TFL disponible). Il n'y a pas de concordance entre les deux analyses pour la position des Poa subg. Arctopoa. De même, les données TFL situent le genre Anisetylon à l'extérieur des Poa, alors que les données ITS le placent dans les Poa. Sur la base de ces résultats et des observations morphologiques, les auteurs reconnaissent les Arctopoa et les Aniselytron comme genres, probablement d'origine hybride ancienne. On crée le nothogenre ×Duarctopoa, contenant une seule nothoespèce ×Duractopoa labradorica. On crée une nouvelle combinaison pour les Poa subg. Sylvestres.
    Full-text · Article · Jul 2008 · Botany
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    Jeffery M. Saarela · Paul M. Peterson · Nancy F. Refulio-Rodriguez
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    ABSTRACT: Bromus ayacuchensis, a new species from Departamento Ayacucho, Peru, is described and illustrated. The new species is similar to species in Bromus sect. Ceratochloa, particularly B. cebddilla, because of its strongly keeled lemmas. It differs from B. cebddilla by having densely pilose culms near the base, adaxially pilose ligules 3-6.6 mm long, lower glumes 1-veined, upper glumes 3-veined, pilose lemmas (especially along the margins and near the apex), lemma margins that are tightly involute on lower 1/5-1/4, and lemma awns 3-5.5 mm long. Phyloge- netic analyses of sequence data from the internal transcribed spacer regions of nuclear ribosomal DNA place B. ayacuchensis in a clade with species of sect. Ceratochloa, whereas analyses of plastid data from the trnL intron and the 3'-end of ndhF indicate that B. ayacuchensis is closely related to this clade. Confirmed records are pro- vided for B. modestus and B. cebadilla in Peru, and B. coloratus and B.flexuosus are reported for the first time in Peru. A key to the 12 species of Bromus now known from Peru is included.
    Full-text · Article · Nov 2005

Publication Stats

276 Citations
7.71 Total Impact Points


  • 2011-2014
    • University of Washington Seattle
      • Department of Biology
      Seattle, Washington, United States
  • 2012
    • Claremont Graduate University
      Клермонт, California, United States
  • 2005-2008
    • Rancho Santa Ana Botanic Garden
      Claremont, California, United States