Publications (2)2.3 Total impact
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ABSTRACT: Mastitis is one of the most important bovine diseases causing economic losses to dairy producers. The research aimed to monitor microbiological and related systemic immunological changes in milking cows raised under semi-intensive conditions, showing signs of mastitis in one or more quarters of the udder, to potentially improve the treatment and increase the number of healed animals. Bacteriological (cultivation and API biochemical identification) and immunological (zinc precipitation technique for total IgG, PEG 4.2% precipitation to quantify circulating immune complexes, carbon particle inclusion test for phagocytosis and in vitro blast transformation technique for leukocyte functions) test were used. Extracts of Echinacea angustifolia, Hippophae rhamnoides, Sylibum marianum, Aloe vera and Thymus vulgaris were tested in vitro for their restoring potential of adaptive cell mediated immunity. Microbiological results indicated te prevalence of Gram positive rods, but only one strain of S. aureus was identified (6.6%), the rest of the strains being classified as S. sciuri, S. xylosus and S. lentus. The blood levels of total Ig were statistically significantly increased (0.079-0.090 ODU, p<0.01-0.001) when compared to those in healthy animals, indicating the increase of systemic humoral response to local antigenic stimulation. CIC values did not exceed the physiological limit (0.004±0.0004 ODU). The phagocytic activity at systemic level was very week (0.01±0.008 to 0.05±0.004) when compared to that observed under physiological circumstances. Stimulation indices in control cultures were lower than normal (36.97±10.03%) as well as the response to the clasical mitogen PHA M. The most effective extracts were the Thymus vulgaris and Aloe vera ones, with a significant stimulatig effect over the untreated control culture (+59.43%, p<0.05 and +49.58%, p<0.05, respectively). The data suggested an impeded systemic response during a localised infection, meaning an increased susceptibility to other potential intercurrent pathogens. The tested vegetal extracts could be of help in increasing systemic immunology.
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ABSTRACT: Lyme disease is a perfect model of the complex relationship between host, vector, and the vector-borne bacteria. Both dogs and horses in Romania are exposed to infection. The aim of the present study was to assess the seroreactivity against Borrelia burgdorferi sensu lato in dogs and horses from different regions of Romania. 276 samples from dogs and 260 samples from horses located in different regions of Romania were analyzed by ELISA and IFA, respectively. The effect of several factors potentially affecting seroreactivity (location, age, gender, occupation, and vector exposition risk) was evaluated using Fisher's exact test (R 2.12.0). The overall prevalence of anti-Borrelia antibodies was 6.52% (18/276) in dogs, with a significantly higher positivity (46.15%, 6/13, p = 0.0005) recorded in a midcountry region. Seroreactivity was correlated with occupation, with working dogs being more exposed. The results may indicate that Lyme borreliosis foci are restricted to small areas, but further studies on Borrelia prevalence in tick populations are needed to confirm this hypothesis. In horses, a global seroprevalence of 11.92% (31/260) was observed. No correlations were found between positive results and age, sex, county, or occupation. This is the first serological survey on antibodies to B. burgdorferi sensu lato in Romanian dogs and horses.
Infectious Diseases Hospital of Cluj-NapocaKlausenburg, Cluj, Romania