Juan Jiang

Qufu Normal University, Küfow, Shandong Sheng, China

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Publications (10)20.98 Total impact

  • Li Jiang · Ruoyi Kang · Li Zhang · Juan Jiang · Zhifang Yu
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    ABSTRACT: Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7 days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P < 0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf.
    No preview · Article · Jun 2015 · Food Chemistry
  • Juan Jiang · Li Jiang · Haibo Luo · Zhifang Yu
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    ABSTRACT: A browning model was proposed to investigate the physiological and biochemical changes of fresh-cut lotus root cv. “3537”. The effects of water (CK), 0.1% (w/v) NaHSO3, 0.2% citric acid (CA), 0.01% ascorbic acid (AA), 0.1% l-cysteine (Cys) and composite preservative (CP: 0.1% CA, 0.005% AA and 0.05% Cys) on the quality and physiology of the lotus root during low-temperature storage were determined. CP treatment minimized browning degree L*, respiration rate and levels of malondialdehyde (MDA), superoxide anion (O2 − 2 2 L * 2 − Lˆ*=65.784−11.062x3−0.101x5+0.003x6 x 3 x 5 2 − x 6
    No preview · Article · Jun 2014 · Postharvest Biology and Technology
  • Li Jiang · Juan Jiang · Haibo Luo · Zhifang Yu
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    ABSTRACT: Effect of nano-packing combined controlled atmosphere (CA + NP) on postharvest physiology and biochemistry of Gynura (Gynura bicolor D.C) was investigated during 20-day storage at 0C. The results showed that the CA + NP had a quite beneficial effect on physicochemical and sensory quality of Gynura compared with nano-packing (NP) or normal polyethylene packaging with a controlled atmosphere (CA). After 20-day storage, decay index was increased to 8.97% for CA + NP, 9.23% for NP and 9.64% for CA, respectively. Relative leakage rate, O2●- production and MDA content of CA + NP were significantly inhibited. Meanwhile, CA + NP effectively reduced the activities of PPO, and enhanced the activities of the enzymes POD, CAT and SOD. These results indicated that CA + NP could provide a promising alternative for extending storage life and improving postharvest quality of Gynura.Practical ApplicationsGynura, a Chinese herbal medicine, has also been used as food substances throughout Asia for centuries. Since the main pharmacological composition on it has not been determined, the fresh Gynura demand increases gradually. However, postharvest decay of Gynura is the main limitation to market acceptance, this study has given an experimental evidence that CA + NP could provide a promising alternative for extending storage life and improving postharvest quality of Gynura. Furthermore, these nano-packing materials have the advantages of simple processing and industrial feasibility in contrast with other storages, some of which are time-consuming and costly.
    No preview · Article · Jun 2014 · Journal of Food Processing and Preservation
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    ABSTRACT: The effect of gibberellic acid (GA3) and 6-benzylaminopurine (6-BA) on lignification of fresh-cut Zizania latifolia were investigated for 15 days at 1C. Results showed that treatment of Z. latifolia slices with GA3 and 6-BA slowed down the increase in cellulose and lignin contents, maintained relatively high cellulase (CEL) activities and total phenol contents. Both treatments also inhibited phenylalanine ammonia-lyase, 4-coumarate CoA ligase, cinnamyl alcohol dehydrogenase and peroxidase activities. GA3 treatment promoted the accumulation of superoxide anion radical (O2·-) and hydrogen peroxide (H2O2) over the first 9 days of storage but reduced their accumulation after 9 days of storage. 6-BA treatment significantly inhibited O2- and H2O2 accumulation throughout storage period. These results indicated that GA3 and 6-BA could retard lignification thereby maintaining a better texture of fresh-cut Z. latifolia during storage at 1C, which provides a theoretical basis for commercial application. The marketing of packaged and refrigerated fresh-cut Zizania latifolia is a choice of increasing interest for vegetables processors. However, fresh-cut Z. latifolia have a faster rate of lignification during retail because of wounding. Therefore, this study was conducted to develop treatments to reduce tissue lignification of fresh-cut Z. latifolia. Results showed that GA3 and 6-BA treatments can effectively inhibit lignification in fresh-cut Z. latifolia, which provides a theoretical basis for commercial application.
    No preview · Article · Oct 2013 · Journal of Food Processing and Preservation
  • Haibo Luo · Li Jiang · Li Zhang · Juan Jiang · Zhifang Yu
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    ABSTRACT: The quality changes of whole and fresh-cut Zizania latifolia were investigated for 12 days at 1 °C. The results showed that the respiratory rate of whole Z. latifolia increased over the first 9 days of storage and then decreased quickly. Weight loss and cellulose and lignin contents increased, while whiteness index (WI), firmness, and total sugar content diminished. Polyphenol oxidase (PPO) and peroxidase (POD) activities decreased for the first 3 days and then increased, whereas the activities of SOD, APX, and CAT increased and reached a peak value on the 3rd, 6th, and 9th days, respectively. Fresh-cut exhibited higher respiratory rate, weight loss, and cellulose and lignin contents and lower WI, firmness, and total sugar content; activities of PPO, POD, superoxide dismutase, ascorbate peroxidase, and catalase were enhanced compared with whole Z. latifolia. However, most of these quality attributes were similar for cut and whole Z. latifolia samples during the first 6 days of storage, suggesting that storage at 1 °C delayed quality deterioration thereby extending the shelf life of packaged fresh-cut Z. latifolia.
    No preview · Article · Dec 2012 · European Food Research and Technology
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    ABSTRACT: Peroxidase (POD) from fresh‐cut Zizania latifolia was purified using a combination of (NH4)2SO4 fractionation and anion exchange chromatography, resulting in one cationic (PODc) and two anionic fractions (PODa I and II). The cationic fraction, which accounted for 76% of recovered activity, was further purified by gel filtration. The POD activity was purified 61.51‐fold with 9.32% recovery. The purified enzyme contains three POD isoenzymes (PODc1–3) as estimated by native polyacrylamide gel electrophoresis and showed molecular weights of 20.6–22.1 kDa on sodium dodecyl sulphate polyacrylamide gel electrophoresis. The optimum pH and temperature were 6.0 and 40C, respectively. The enzyme was stable in the pH range 4.0–7.0 while retained only 2.21% of the activity after 1 min at 60C. The Km for guaiacol and H2O2 were 10 mM and 0.15% (v/v), respectively. Influence of various chemicals on the enzyme activity were studied. These results provide a theoretical basis for inhibiting browning and lignification of fresh‐cut Z. latifolia. PRACTICAL APPLICATIONSThe marketing of packaged and refrigerated fresh‐cut Zizania latifolia is a choice of increasing interest for vegetables processors. However, cut surface browning and tissue lignification in fresh‐cut Z. latifolia are two major causes of loss in quality. It has been reported that peroxidase (POD) may be involved in enzymatic browning and lignification. This study investigated the purification and characterization of POD from fresh‐cut Z. latifolia. Results showed that heat treatment and/or chemical dip (L‐cysteine, N‐acetyl‐L‐cysteine and ascorbic acid) might be suitable techniques for inhibiting browning and lignification of the fresh‐cut Z. latifolia.
    No preview · Article · Jun 2012 · Journal of Food Biochemistry
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    ABSTRACT: Browning is a critical problem, which often limits the shelf life and marketability in fresh-cut lotus tuber. Proteome level changes in response to the browning metabolism were investigated using two-dimensional electrophoresis (2-DE) and MALDI-TOF-TOF. A total of 34 functional protein spots were identified by comparing 2-DE protein patterns of fresh-cut lotus tuber before and after browning. These 34 identified proteins could be classified into 7 functional groups based on the NCBI database, that is, material and energy metabolism (35%), stress response (20%), respiration metabolism (12%), cell structure (12%), signal transduction (6%), gene expression regulation (6%), and unclassified proteins (9%). The group with the greatest difference in protein expression was related to material metabolism and regulation, reactive oxygen species metabolism, and respiratory control. The distinct proteins included universal stress protein (USP), superoxide dismutase (SOD), peroxidase (POD), ferritin, and ATPase.
    No preview · Article · Apr 2012 · Journal of Agricultural and Food Chemistry
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    ABSTRACT: Based on the core skeleton of the total synthesized bisbibenzyl marchantin C, riccardin D and plagiochin E, a series of brominated and aminomethylated derivatives of above three bisbibenzyls have been synthesized and their cytotoxic activity against KB, MCF-7 and PC3 cell lines has been preliminary evaluated. The bio-test results revealed that the brominated derivatives 21, 22, 24, 25 and 28 exhibited excellent antiproliferative activity, with IC(50) value lower than their parent compounds. As a most potent microtubule depolymerization agent, compound 28 was found to arrest cells at the G(2)/M phase of the cell cycle as determined by the flow cytometry assay in PC3 cell line. The remarkable biological profile and novel structure of these bisbibenzyl derivatives make them possible as promising candidates for clinical development as chemotherapeutic agents.
    No preview · Article · Feb 2012 · Bioorganic & medicinal chemistry
  • Li Zhang · Zhifang Yu · Li Jiang · Juan Jiang · Haibo Luo · Linran Fu
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    ABSTRACT: The extracted proteins from the heat-treated peach fruit (dipped in hot water at 48°C for 10min and then stored at room temperature (20°C-25°C) for up to 6 days) were used for proteomic analysis in order to understand the response of post-harvest peach fruit to heat treatment during ripening stage at proteomic level. After two dimensional gels electrophoresis (2-DE) was conducted, more than 600 protein spots were detected. Among them, 35 differently expressed spots (P<0.05) were selected to be excised and analyzed using MALDI-TOF/TOF, and finally 30 protein spots were confidently identified according to NCBI database. The results demonstrated that among the thirty protein spots expressed particularly induced by heat treatment, 43% were related to stress response, 17% to cell structure, 13% to protein fate, 7% to glycolytic pathway, 3% to ripening and senescence and 17% to unclassified. All of them are involved in the regulation of peach fruit development and ripening. All these indicated that the self-defense capability of peach fruit was improved by heat treatment. The study will enable future detailed investigation of gene expression and function linked with peach fruit ripening.
    No preview · Article · Jun 2011 · Journal of proteomics
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    ABSTRACT: Effects of temperature and packaging on the decay and physiology of Gynura bicolor D.C were investigated using selected fresh leaves of G. bicolor packed (half opened, half closed) in plastic bags, and then stored at 0C, 10C and 20C, respectively. The results showed that less water loss and tissue decay, smaller change of peroxidase activity and malondialdehyde and O2-content, slower respiratory and relative leakage rate, higher catalase (CAT) activity and superoxide dismutase (SOD) activity were found in G. bicolor stored at 0C. Closed packaging could somewhat benefit low temperature effect on the weight loss, tissue decay, CAT and SOD activity. Therefore, 0C combined with closed packaging was sufficient to extend storage life to 20 days and prevent decay of G. bicolor. Gynura bicolor D.C is nourishing but just originally grown in the southern part of China. Closed packaging combined with low temperature is an efficient technique to be used for keeping high quality of leaves, extending storage life and preventing decay of the leaves of G. bicolor. Therefore, this technique is appropriate to store and transport G. bicolor, and could also be recommendable to other leafy vegetables.
    No preview · Article · Jul 2010 · Journal of Food Processing and Preservation

Publication Stats

91 Citations
20.98 Total Impact Points


  • 2014-2015
    • Qufu Normal University
      Küfow, Shandong Sheng, China
  • 2010-2014
    • Nanjing Agricultural University
      • College of Food Science and Technology
      Nan-ching, Jiangsu, China
  • 2012
    • Shandong University
      • School of Pharmaceutical Sciences
      Chi-nan-shih, Shandong Sheng, China