Wibool Piyawattanametha

King Mongkut's Institute of Technology Ladkrabang, Siayuthia, Bangkok, Thailand

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Publications (82)116.38 Total impact

  • Zhen Qiu · Wibool Piyawattanametha
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    ABSTRACT: Progress toward early diagnosis of cancer would have significant clinical benefits in reducing mortality or prolonging life in cancer patients; thus, there is an important unmet clinical need to image cellular features of cancer in vivo and in real time to correlate pathological symptoms and underlying cells responsible for such symptoms. In this paper, we describe a review of microelectromechanical systems scanners-based endoscopic optical coherence tomography, confocal, two-photon, and photoacoustic microscopy imaging. These advanced optical imaging modalities can provide subcellular (micron-scale) resolution and deep tissue penetration to reveal both cells and molecular features for early cancer diagnosis, cancer staging, and surgical guidance.
    No preview · Article · Jul 2015 · IEEE Journal of Selected Topics in Quantum Electronics
  • S. Pattanasak · W. Piyawattanametha
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    ABSTRACT: Progress toward early diagnosis of cancer would have important clinical benefits in reducing cancer mortality; thus, there is an urgent need to improve cancer screening by providing in vivo real-time high-resolution diagnosis through optical imaging modalities. Microelectromechanical systems (MEMS) based optical endoscopes have been playing a key role in miniaturization of fiber-optical endoscopy for cancer diagnosis for over 30 years. In this paper, both review and technology outlook of MEMS based fiber-optical microendoscopy for real-time in vivo disease diagnosis will be provided.
    No preview · Article · Jul 2015
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    ABSTRACT: Protein p(16INK4a) (p16) is a well-known biomarker for diagnosis of human papillomavirus (HPV) related cancers. In this work, we identify novel p16 binding peptides by using phage display selection method. A random heptamer phage display library was screened on purified recombinant p16 protein-coated plates to elute only the bound phages from p16 surfaces. Binding affinity of the bound phages was compared with each other by enzyme-linked immunosorbent assay (ELISA), fluorescence imaging technique, and bioinformatic computations. Binding specificity and binding selectivity of the best candidate phage-displayed p16 binding peptide were evaluated by peptide blocking experiment in competition with p16 monoclonal antibody and fluorescence imaging technique, respectively. Five candidate phage-displayed peptides were isolated from the phage display selection method. All candidate p16 binding phages show better binding affinity than wild-type phage in ELISA test, but only three of them can discriminate p16-overexpressing cancer cell, CaSki, from normal uterine fibroblast cell, HUF, with relative fluorescence intensities from 2.6 to 4.2-fold greater than those of wild-type phage. Bioinformatic results indicate that peptide 'Ser-His-Ser-Leu-Leu-Ser-Ser' binds to p16 molecule with the best binding score and does not interfere with the common protein functions of p16. Peptide blocking experiment shows that the phage-displayed peptide 'Ser-His-Ser-Leu-Leu-Ser-Ser' can conceal p16 from monoclonal antibody interaction. This phage clone also selectively interacts with the p16 positive cell lines, and thus, it can be applied for p16-overexpressing cell detection. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd.
    No preview · Article · Mar 2015 · Journal of Peptide Science
  • Zhen Qiu · Wibool Piyawattanametha
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    ABSTRACT: Fiber-optical microendoscopy has recently been an essential medical diagnostic tool for patients in investigating tissues in vivo due to affordable cost, high quality imaging performance, compact size, high-speed imaging, and flexible movement. Microelectromechanical systems (MEMS) scanner technology has been playing a key role in shaping the miniaturization and enabling high-speed imaging of fiber-optical microendoscopy for over 20 years. In this article, both review of MEMS based fiber-optical microendoscopy for optical coherence tomography, confocal, and two-photon imaging will be discussed. These advanced optical endoscopic imaging modalities provide cellular and molecular features with deep tissue penetration enabling guided resections and early cancer assessment.
    No preview · Article · Jan 2015 · Displays
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    C-B Wong · B-Y Khoo · S Sasidharan · W Piyawattanametha · S H Kim · N Khemthongcharoen · M-Y Ang · L-O Chuah · M-T Liong
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    ABSTRACT: Increasing levels of antibiotic resistance by Staphyloccocus aureus have posed a need to search for non-antibiotic alternatives. This study aimed to assess the inhibitory effects of crude and fractionated cell-free supernatants (CFS) of locally isolated lactic acid bacteria (LAB) against a clinical strain of S. aureus. A total of 42 LAB strains were isolated and identified from fresh vegetables, fresh fruits and fermented products prior to evaluation of inhibitory activities. CFS of LAB strains exhibiting a stronger inhibitive effect against S. aureus were fractionated into crude protein, polysaccharide and lipid fractions. Crude protein fractions showed greater inhibition against S. aureus compared to polysaccharide and lipid fractions, with a more prevalent effect from Lactobacillus plantarum 8513 and L. plantarum BT8513. Crude protein, polysaccharide and lipid fractions were also characterised with glycine, mannose and oleic acid being detected as the major component of each fraction, respectively. Scanning electron microscopy revealed roughed and wrinkled membrane morphology of S. aureus upon treatment with crude protein fractions of LAB, suggesting an inhibitory effect via the destruction of cellular membrane. This research illustrated the potential application of fractionated extracts from LAB to inhibit S. aureus for use in the food and health industry.
    Full-text · Article · Sep 2014 · Beneficial Microbes
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    ABSTRACT: This study aimed to evaluate anti-staphylococcal properties of crude and fractionated extracts of lactic acid bacteria (LAB) isolated from local meat, dairy, and fermented products. A total of 36 LAB isolates were obtained and identified via 16S rDNA sequencing. Cell-free supernatant (CFS) of all isolates exhibiting a statistically significant inhibition against Staphylococcus aureus (ρ < 0.05), with six LAB isolates exhibiting a more prevalent inhibition. The inhibition effects of cell wall and intracellular extracts from the six prevalent isolates were evaluated. Lactobacillus plantarum USM8613 was the most prominent isolate with both CFS and cell wall extract exhibiting the most prevalent inhibition against S. aureus. Scanning electron micrographs showed alteration of S. aureus membrane morphology upon CFS treatment, suggesting an anti-staphylococcal effect via membrane destruction. Confocal laser scanning micrographs showed inhibition against biofilm formations by S. aureus in porcine skins upon CFS treatment. The CFS from L. plantarum USM8613 was separated into protein, lipid, and polysaccharide fractions for evaluation of anti-staphylococcal activity and chemical characterization. All fractions inhibited growth of S. aureus (ρ < 0.05), with protein fractions exhibiting stronger inhibition effect. Data from our present study showed that extracts from LAB could be applied as biopreservatives in the food industries and/or as an antimicrobial agent against bacterial infections for cosmeceutical and pharmaceutical uses. © 2014, Springer-Verlag Berlin Heidelberg and the University of Milan.
    Full-text · Article · Jun 2014 · Annals of Microbiology
  • C. Zhao · Y.-K. Lee · R. Xu · C. Liang · D. Liu · W. Ma · W. Piyawattanametha · Y. Zohar
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    ABSTRACT: Cancer is a leading cause of mortality worldwide causing human deaths. Circulating tumor cells (CTCs) are cells that have detached from a primary tumor and circulate in the bloodstream; they may constitute seeds for subsequent growth of additional tumors (metastasis) in different tissues. The detection of CTCs may have important prognostic and therapeutic implications but, because their number is very small, these cells are not easily detected. Circulating tumor cells are found in the order of 10-100 CTCs per mL of whole blood in patients with metastatic disease. Isolation of tumor cells circulating in the blood stream, by immobilizing them on surfaces functionalized with bio-active coating within microfluidic devices, presents an interdisciplinary challenge requiring expertise in different research areas: cell biology, surface chemistry, fluid mechanics and microsystem technology. We first review the fundamental of cell biology of CTCs and summarize the key microfluidic techniques for isolation of CTCs via cell-ligand interactions, magnetic interactions, filtration; detection and enumeration of CTCs; in vivo CTCs imaging.
    No preview · Article · Jan 2014
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    ABSTRACT: Our work demonstrates a MEMS based handheld dual-axis confocal microscope for cervical cancer screening. Imaging demonstration is performed with plant and animal tissue biopsies. The data is collected and displayed in real time with 2-5 Hz frame rates.
    No preview · Conference Paper · Oct 2013
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    ABSTRACT: A new chapter in the history of medical diagnosis happened when the first X-ray technology was invented in the late 1800s. Since then, many non-invasive and minimally invasive imaging techniques have been invented for clinical diagnosis to research in cellular biology, drug discovery, and disease monitoring. These imaging modalities have leveraged the benefits of significant advances in computer, electronics, and information technology and, more recently, targeted molecular imaging. The development of targeted contrast agents such as fluorescent and nanoparticle probes has made it possible to selectively view specific biological events and processes in both in vivo and ex vivo systems with great sensitivity and selectivity. Thus, these contrast agents or targeted molecular probes have become a mainstay in modern medicinal and biological research. Many promising results have showed potentials to translate to clinical applications. In this review, we describe a discussion of employing imaging probes and optical microendoscopic imaging techniques for cancer diagnosis to enable favorable treatment outcome.
    No preview · Article · Oct 2013 · Advanced drug delivery reviews
  • Ming C. Wu · J.-C. Tsai · Wibool Piyawattanametha · Pamela R. Patterson
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    ABSTRACT: Micro-electro-mechanical systems (MEMS) technology ignites major breakthroughs in several research areas. Optics/photonics is one of these research fields impacted by MEMS techniques. Generally, microoptical elements with sizes ranging from a few microns to a few millimeters belong to the category of optical MEMS. They are inherently suited for cost effective wafer scale manufacturing as the processes are derived from the semiconductor industry. The advantages of applying microelectronics technology to silicon micromechanical devices were presented by Petersen in his classic paper, ‘Silicon as a Mechanical Material’[1]. The ability to steer or direct light is one of the key requirements in optical MEMS. In the past two decades since Petersen published his silicon scanner[2], the field of optical MEMS has experienced explosive growth[3,4]. In the 80’s and early 90’s, displays were the main driving force for the development of micromirror arrays. Portable digital displays are common places and head mount displays are now commercially available. In the past decade, telecommunications has become the market driver for Optical MEMS. The demand for routing internet traffic through fiber optic networks pushes the development of both digital and scanning micromirror systems for large port-count all-optical switches. In the biomedical arena, microoptical scanners promise low-cost endoscopic 3D imaging systems for in vivo diagnostics. Thanks to the ongoing improvement of fabrication technologies, nano-electro-mechanical systems (NEMS) have become feasible and have steadily attracted attention in the fields of optics and photonics. Nano-photonics is particularly promising in guided-wave devices as the structural dimensions (a few hundred nanometers) are close to the optical wavelengths of interest. This chapter summarizes the state of the art of Optical MEMS and nano-photonics technologies and applications. It is organized into the following sections: Section 10.1 describes the generic actuation mechanisms commonly used for MEMS and NEMS devices. Section 10.2 discusses the applications, dividing into three categories including ‘Display, Imaging, and Microscopy,’ ‘Optical Communication,’ and ‘NanoPhotonics.’
    No preview · Article · Aug 2013
  • Wibool Piyawattanametha
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    ABSTRACT: Progress toward early diagnosis of cancer would have important clinical benefits in reducing cancer mortality; thus, there is an important need to image cellular features of cancer in vivo and in real-time. In this paper, we describe a review of microelectromechanical systems (MEMS) scanner based endoscopic optical coherence tomography (OCT), two-photon (2P), and confocal imaging. These modalities can provide submicron- and micro-scale resolution to reveal both cells and molecular features of early cancer diagnosis.
    No preview · Conference Paper · Aug 2013
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    ABSTRACT: A MEMS based handheld multi-spectral confocal microscope has been developed as a noninvasive imaging instrument for cervical cancer screening. Tissue imaging depth of up to 400 μm was demonstrated. The maximum speed of image collection is up to 10 Hz with field of view (FOV) around 200×158 pixel2 size. Our handheld prototype is able to visualize cancer cells with cellular resolution (transverse resolution = 5 μm and axial resolution = 6 μm). Biological imaging demonstration was carried out on cell culture and tissues of human cervix.
    No preview · Conference Paper · Jun 2013
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    ABSTRACT: In this paper, two different designs of two-dimensional MEMs scanner were tested to find their characteristic responses in dynamic mode by applying a fixed voltage at different electrical biasing waveforms: sinusoidal, triangle, square and saw-tooth waveforms. The collected data from the experiment has proven that the significance of MEMS scanner design and driving input signals.
    No preview · Conference Paper · Dec 2012
  • Numfon Khemthongcharoen · Athisake Ruangpracha · Wibool Piyawattanametha
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    ABSTRACT: This work demonstrates the application of phage display technology for molecular diagnosis utility. We propose a novel phage-displayed peptide which specifically bind to p16INK4a, a cervical cancer biomarker. Whole phage particles were developed as a molecular tracer for ex vivo cells imaging technique. Increase in specific phages binding to p16INK4a overexpressed cells is improved when the cells were initially permeabilized in order to make phage penetrable pores on the target cell membranes. We also proved that fluorescence signal could be obviously enhanced due to tremendous interaction sites for fluorescence dye labeling available on capsid proteins around phage particles. Evaluation of p16INK4a binding phages to discriminate between p16INK4a overexpressed cervical cancer cells versus normal fibroblast cells demonstrated higher fluorescence intensity of 2.5 fold over native phages.
    No preview · Conference Paper · Nov 2012
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    ABSTRACT: We describe a 2-D MEMS scanner for a handheld multispectral confocal microscope for early detection of cervical cancer. The MEMS scanner has an inner gimbal design with torsional springs separated from the reflectors to reduce light loss while maintaining chip size to 3.25 × 3.25 mm2. The devices are large-scale batch fabricated using a double layer SOI process. The scanner has electrostatic optical deflection angles of 3.25° for the inner axis at 75 V and ±1.6° for the outer axis at 60 V. The device has resonance frequencies of 2.84 kHz and 452 Hz for the inner and outer axis torsional modes respectively.
    No preview · Conference Paper · Aug 2012
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    ABSTRACT: We demonstrated a handheld multispectral fluorescence confocal microscope for cervical cancer diagnostic using dual-axis confocal microscope architecture and a microelectromechanical systems scanner. The real time images are acquired with frame rate up to 15 Hz.
    No preview · Conference Paper · Aug 2012

  • No preview · Conference Paper · Jul 2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Our work demonstrated a MEMS based handheld dual-axis confocal microscope for cervical cancer screening. Imaging demonstration is performed with plants and animal tissue biopsies. The data is collected and displayed in real time with 2-5 Hz.
    No preview · Conference Paper · May 2012
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    ABSTRACT: We present a 9.6-mm fiber-coupled probe for femtosecond laser microsurgery and nonlinear imaging. Towards enabling clinical use, we successfully reduced the volume of our earlier 18-mm surgery probe by 5 times, while improving optical performance.
    Full-text · Article · Apr 2012
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    ABSTRACT: Near-infrared confocal microendoscopy is a promising technique for deep in vivo imaging of tissues and can generate high-resolution cross-sectional images at the micron-scale. We demonstrate the use of a dual-axis confocal (DAC) near-infrared fluorescence microendoscope with a 5.5-mm outer diameter for obtaining clinical images of human colorectal mucosa. High-speed two-dimensional en face scanning was achieved through a microelectromechanical systems (MEMS) scanner while a micromotor was used for adjusting the axial focus. In vivo images of human patients are collected at 5 frames/sec with a field of view of 362×212 μm(2) and a maximum imaging depth of 140 μm. During routine endoscopy, indocyanine green (ICG) was topically applied a nonspecific optical contrasting agent to regions of the human colon. The DAC microendoscope was then used to obtain microanatomic images of the mucosa by detecting near-infrared fluorescence from ICG. These results suggest that DAC microendoscopy may have utility for visualizing the anatomical and, perhaps, functional changes associated with colorectal pathology for the early detection of colorectal cancer.
    Full-text · Article · Feb 2012 · Journal of Biomedical Optics

Publication Stats

1k Citations
116.38 Total Impact Points


  • 2015
    • King Mongkut's Institute of Technology Ladkrabang
      • Faculty of Engineering
      Siayuthia, Bangkok, Thailand
  • 2010-2015
    • Chulalongkorn University
      • Faculty of Medicine
      Krung Thep, Bangkok, Thailand
  • 2006-2012
    • Stanford University
      • • Department of Electrical Engineering
      • • School of Engineering
      • • Department of Applied Physics
      Stanford, California, United States
  • 2007
    • University of California, Berkeley
      • Department of Electrical Engineering and Computer Sciences
      Berkeley, California, United States
  • 2000-2006
    • University of California, Los Angeles
      • Department of Electrical Engineering
      Los Angeles, California, United States