[Show abstract][Hide abstract] ABSTRACT: A polyphasic analysis was undertaken of seven independent isolates of gram-negative cocci collected from pathological clinical samples from New York, Louisiana, Florida, Illinois and healthy subgingival plaque from a patient in Virginia. The 16S rRNA gene sequence similarity among these isolates is 99.7 to 100% and the closest species with a valid name is Neisseria lactamica (96.9% similarity). DNA-DNA hybridization confirms that these isolates are of the same species and are distinct from their nearest phylogenetic neighbor, N. lactamica. Phylogenetic analysis of 16S and 23S rRNA genes indicates the new species belongs in the genus Neisseria. The predominant cellular fatty acids (CFA) are 16:0, summed feature 3 (16:1, ω7c/15 iso 2OH), and 18:1 ω7c. The CFA profile, together with other phenotypic characters, further supports inclusion of the new species in the genus. The name Neisseria oralis sp.nov. (type strain 6332(T) =DSM 25276(T) =LMG 26725(T)) is proposed.
Full-text · Article · Jul 2012 · International Journal of Systematic and Evolutionary Microbiology
[Show abstract][Hide abstract] ABSTRACT: Twelve independent isolates of a gram-positive, endospore-forming rod were recovered from clinical specimens in New York State, USA, and from raw milk in Flanders, Belgium. The 16S rRNA gene sequences for all isolates were identical. The closest species with a validly published name, based on 16S rRNA gene sequence, is Sporosarcina koreensis (97.13 % similarity). DNA-DNA hybridization studies demonstrate that the new isolates belong to a species distinct from their nearest phylogenetic neighbours. The partial sequences of the 23S rRNA gene for the novel strains and their nearest neighbours also provide support for the novel species designation. Maximum-likelihood phylogenetic analysis of the 16S rRNA gene sequences confirmed that the new isolates are in the genus Sporosarcina. The predominant menaquinone is MK-7, the peptidoglycan has the type A4α L-Lys-Gly-D-Glu, and the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant fatty acids are iso-C(14 : 0), iso-C(15 : 0) and anteiso-C(15 : 0). In addition, biochemical and morphological analyses support designation of the twelve isolates as representatives of a single new species within the genus Sporosarcina, for which the name Sporosarcina newyorkensis sp. nov. (type strain 6062(T) = DSM 23544(T) = CCUG 59649(T) = LMG 26022(T)) is proposed.
Full-text · Article · Mar 2011 · International Journal of Systematic and Evolutionary Microbiology