[Show abstract][Hide abstract] ABSTRACT: Ultrastructural and ultracytochemical characteristics of glandular epithelium of endometrium in 10 women with two-phase cycle were studied. Secretory transformation of glandular epithelium at ultrastructural level is manifested by deposits of glycogen in cytoplasm, giant mitochondria, hypertrophic Golgi complex and nuclear channel system. In organ endometrial cultures formation of the nuclear channel system at the stage of proliferation is induced by progesterone. Localization of ribonucleoproteins and activity of nucleoside phosphatases (ATPase and 5'-nucleotidase) in the nuclei and the channel system is described. Ultrastructural and ultracytochemical data permit to exclude nucleolar origin of the nucleolar channel system.
No preview · Article · Sep 1986 · Arkhiv anatomii, gistologii i émbriologii
[Show abstract][Hide abstract] ABSTRACT: Morphological and ultrastructural changes of cells in atypical hyperplasia and focal adenomatosis of the endometrium considered to be precancer processes as well as of endometrial adenocarcinoma before and after treatment (in organ culture) with progesterone and norethysterone were studied. In atypical hyperplasia and focal adenomatosis (after 3-day incubation with hormone), early signs of secretory transformation of the glandular epithelium were observed including subnuclear vacuoles containing glycogen, reduction of microvilli, giant mitochondria, crimping of the cellular membrane, complication of the endoplasmic reticulum. In cancer tissue cultures the hormonal effect is manifested at the ultrastructural level only: signs of morphological differentiation of tumor cells were observed. In these pathological conditions, endometrial organ cultures may be used for the determination of hormone sensitivity of the tumor.
No preview · Article · Feb 1982 · Arkhiv patologii
[Show abstract][Hide abstract] ABSTRACT: Ultracytochemistry was used to study and compare cytochromooxidase, succinate dehydrogenase and NADH-dehydrogenase activity in gastric mucosa parietal cells in health and in gastric carcinoma associated with decreased acidity of gastric juice. The study demonstrated the reduced activity of the enzymes listed in the mucosal parietal cells in gastric carcinoma. This finding is interpreted as a consequence of disturbed energy supply of hydrochloric acid secretion in gastric carcinoma.
No preview · Article · Jul 1981 · Biulleten' eksperimental'noĭ biologii i meditsiny
[Show abstract][Hide abstract] ABSTRACT: The report deals with the ultrastructural cell patterns of gastric carcinoma and electron-microscopy study of the histochemical features of cells. The histochemical features of tumor cells were compared with homologous normal epithelial cells. The localization and activity of ATP-ase, IDP-ase, acid and alkaline phosphatases were studied. The data obtained suggest a structural and functional reorganization in the cells of human stomach tumors. It was also found that the histological and ultrastructural differentiation and the degree of functional maturity of tumor cells do not necessarily coincide.
No preview · Article · Feb 1981 · Voprosy onkologii
[Show abstract][Hide abstract] ABSTRACT: Ultracytochemical study was made of inosine diphosphatase (IDPase) and adenosine triphosphatase (ATPase) in the nuclei of normal epithelial and cancer cells of human gastric tumors. A new incubation medium for ultracytochemical demonstration of IDPase activity in the cell nuclei was developed. The activity of IDPase and ATPase in the nucleoplasm and in the nucleoli of the tumor cells was shown to be lower than in respective normal cells. IDPase activity in the nuclear envelope in the tumor cells was absent in contrast to the normal cells.
No preview · Article · Aug 1980 · Biulleten' eksperimental'noĭ biologii i meditsiny
[Show abstract][Hide abstract] ABSTRACT: An electron-histochemical investigation was made of ATPase in the partietal cells of the mucous membrane and in tumor cells of an adenocarcinoma of the human stomach, with similar ultrastructure. The reaction product for ATPase in the parietal cells was found on membranes of microvilli of the intracellular tubules, on membranes bounding the lateral intercellular spaces, on the basal plasmalemma, and in the nucleoli. No reaction product was found on membranes of the tubulovesicles or on the apical surface of the plasmalemma. The reaction product was observed in the tumor cells on membranes of the villi of the intracellular tubules, on the basal plasmalemma, and in the nucleoli. Comparison of ATPase activity in these cells showed that the part of the mechanism of hydrochloric acid secretion that is connected with H+ and Cl- transport is preserved in the tumor cells. The permanent decrease in hydrochloric acid secretion by the gastric mucosa in the presence of cancer and by the cancer itself is probably attributable to other mechanisms.
No preview · Article · Jul 1978 · Bulletin of Experimental Biology and Medicine
[Show abstract][Hide abstract] ABSTRACT: A study was made of the effect of procedures (freezing-thawing prior to incubation, prefixation with formaldehyde and glutaraldehyde, incubation with DMSO) on the activity of ATPase and beta-glycerophosphatase in leucocytes and erythrocytes of man, and of the effect of these procedures and of homogenization on ATPase activity in the cells of the rat thymus. The homogenization of rat thymocytes decreases ATPase activity by 15%. A repeated freezing-thawing results in a 15% decrease of ATPase activity in the cells of the rat thymus. The homogenization of rat thymocytes decreases ATPase activity in rat thymocytes, in a 2% decrease in human leucocytes, and in a 21% increase in human erythrocytes. Beta-glycerophosphatase activity in leucocytes and in erythrocytes increases thereby by 89 and 38%. Incorporation of 5% DMSO into the medium increases ATPase activity in human leucocytes and erythrocytes by 17 and 16%, while thymocytes this activity drops by 27%. Beta-glycerophosphatase activity increases thereby in leucocytes by 26 and in erythrocytes by 11.5%, resp.
[Show abstract][Hide abstract] ABSTRACT: Ultracytochemical investigation of ATP-ase activity was carried out in parietal cells of the mucosa and in cancer cells of human stomach carcinoma possessing a similar ultrastructure. In parietal cells the reaction product of ATP-ase was observed on the membranes of microvilli of intracellular canaliculi, on the membranes delineating the lateral intercellular space, on the basal plasmolemma and in the nucleoli. The reaction product was absent on the membranes of tubuvesicles and on the apical surface of the plasmalemma. In cancer cells the reaction product was found on the membranes of the microvilli of the intracellular canaliculi, basal plasmolemma and in the nucleoli. Comparative examination of ATP-ase activity in these cells implies that at least the part of the mechanism of hydrochloric acid secretion which is involved in the transfer of H+ and Cl- is retained in cancer cells. A steady decrease in hydrochlorid acid secretion observed in the stomach mucosa in cancer as well as in the tumour itself seems to be associated with other mechanisms.
No preview · Article · Feb 1978 · Biulleten' eksperimental'noĭ biologii i meditsiny
[Show abstract][Hide abstract] ABSTRACT: Electron-microscopic studies of the lymphatic nodes in lympho- and reticulosarcomas of man showed that tumoral cells retained the ultrastructural signs of initial normal cells (lymphoid or reticular) in the degree sufficient for their utilization in differential diagnosis of these two forms of the disease.
No preview · Article · Feb 1977 · Arkhiv patologii
[Show abstract][Hide abstract] ABSTRACT: Activity of acid phosphotase in the urethra discharge in patients with various forms of gonorrhea (acute, torpid, and chronic) was studied with the use of electron-microscopy and biochemical methods. A positive reaction of nuclei of the epithelial cells to acid phosphotase in lysosomes and perichromatin granules was demonstrated. In polymorphononuclear leucocytes the positive reaction to acid phosphotase could be also sometimes observed in granules of the cytoplasm. The electronograms presented testify to heterogeneity and high activity of acid phosphotase in lysosomes. Neither electron-microscopy nor biochemical methods could help reveal any differences in the activity of acid phosphotase in various forms of gonorrhea.
No preview · Article · Feb 1976 · Arkhiv patologii
[Show abstract][Hide abstract] ABSTRACT: Ultrastructural localization of some oxidative and dephosphorylating enzymes was investigated in isolated rat and mouse liver nuclei and in nuclei isolated from various rat hepatomas. In the discussion the significance of changes in the activity of these enzymes in neoplastic cells is considered.
No preview · Article · Feb 1976 · Folia histochemica et cytochemica