H Kamano

Kagawa University, Takamatu, Kagawa, Japan

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Publications (24)58.79 Total impact

  • Y. Imai · E. Miyazaki · H. Kamano
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    ABSTRACT: This paper focuses on System design and Evaluation of "Automatic Health Screening System", which can play an important role of Health Education Support System for University. Major characteristics of This health screening system has some major characteristics such as using IC card for user identification, interfacing several kinds of physical measuring devices and retrieving measured data through Web-DB system. In this case, measuring devices we assumed are, for example, height meter, weight meter, blood pressure monitor, vision analyzer, and so on. Automatic identification for examinees can reduce human errors and incorrect recognition by means of IC card. Interfacing physical measuring devices and control-oriented computer can realize speedup of health screening time. Web-DB system and distributed campus network environment can allow effective and efficient retrieval of measured data obtained from health screening system. With such an automatic health screening system, almost students can easily participate in University-level health screening and investigate/retrieve their proper physical data for their healthcare through dedicated Web-DB system on the network environment. Additionally, Consultation Rate of routine physical examination has been improved as an effect of introduction of automatic health screening system. With comparison of the according consultation rates in 2011 and 2012, the latter has been more 20.0% improved than the former.
    No preview · Conference Paper · Jan 2013
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    ABSTRACT: A Health Education Support System has been designed and being implemented for our students of Kagawa University in Japan. This paper describes the detail of An Automatic Health Screening System, which can play the former part role of our Health Education Support System, is described in detail in this paper. Major characteristics of our screening system are using IC card for user identification, interfacing several kinds of physical measuring devices like as height meter, weight meter, blood pressure monitor, etc. and retrieving measured data through Web-DB system. Human errors and incorrect identification can be reduced by means of automatic identification with IC card. Speedup of screening can be realized through interfacing between physical measuring devices and computers. And efficient retrieval of measured data can be performed with Web-DB system and Distributed campuses network environment in our University. With our automatic health screening system, almost students can easily participate in University-level health screening and investigate/retrieve their proper physical data for their healthcare through dedicated Web-DB system on the network environment.
    No preview · Article · May 2012
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    ABSTRACT: A Health Education Support System has been being developed for Students and university staffs of Kagawa University. The system includes an IC card reader(writer), several types of physical measuring devices (height meter, weight meter, blood pressure monitor, etc. for health examination), a special-purpose PC, distributed information servers and campus network environment. We have designed our prototype of a Health Education Support System as follows; Students and/or university staffs can utilize the above system for their health education and/or healthcare whenever they want anywhere in university. They can use IC-based ID cards for user authentication, operate the physical measuring devices very much simply, and maintain their physical data periodically. Measured data can be obtained at any point of university by means of measuring devices connected with the system on-line, transferred through campus network environment, and finally cumulated into a specific database of secured information servers. We have carried out some experiments to design our system and checked behaviour of each subsystem in order to evaluate whether such a system satisfy our requirements to build facilities to support health education described above. In this paper, we will introduce our design concepts of a Health Education Support System, illustrate some experimental results and discuss perspective problems as our summaries.
    No preview · Conference Paper · Jun 2011
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    ABSTRACT: In 2007, a large outbreak of pertussis occurred at a university in Japan. Initially, a student, suffering from nocturnal cough and post-tussive vomiting for 3 weeks was diagnosed with pertussis. During the subsequent outbreak, 361 university students and staff members presented with a primary complaint of a cough. In the present study, we analyzed bacterial agglutinin titers against two Bordetella pertussis strains, Yamaguchi (epidemic strain) and Tohama (vaccine strain), in 310 patients with a cough and evaluated its diagnostic accuracy for adolescent and adult pertussis. These serological analyses showed a significant difference (P<0.001) in the levels of Yamaguchi agglutinin titer, but not in those of Tohama agglutinin titer, between patient and healthy adult groups. Therefore, the bacterial agglutination assay against strain Yamaguchi may be a useful tool for diagnosis of adolescent and adult pertussis, especially in young adults, when an agglutinin titer cutoff value of >or=160x is used in combination with clinical symptoms and other clinical laboratory tests.
    Preview · Article · Mar 2010
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    Preview · Article · Dec 2008 · International Journal of Infectious Diseases
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    ABSTRACT: Bifid intrathoracic rib is a very rare anomaly of the ribs that is characterized by an osseous prominence of a rib into the thoracic cavity. We report a 21-year-old woman with bifid intrathoracic rib arising from the anterior-lateral portion of a depressed 4th rib, based on findings from chest radiography and computed tomography (CT). This is only the second reported case of this type of intrathoracic rib worldwide. We discuss differential diagnoses for this case and suggest a classification of intrathoracic rib from the perspective of morphology and developmental biology.
    No preview · Article · Feb 2006 · Internal Medicine
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    ABSTRACT: In this study, we examined the effect of thrombopoietin (TPO) on the aggregation of platelets from 40 patients with myeloproliferative disorders (MPDs), including 17 patients with chronic myelogenous leukemia in the chronic phase (CML-CP), 10 with polycythemia vera, 10 with essential thrombocythemia, and three with myelofibrosis. TPO by itself dose-dependently induced the aggregation of platelets from patients with CML-CP but not from those with other MPDs or with CML-CP in cytogenetical complete remission. The expression of CD63 in CML-CP platelets was induced by TPO treatment. Phosphatidylinositol 3-kinase (PI3-kinase) was constitutively activated in CML-CP platelets. Pretreatment with PI3-kinase inhibitors (wortmannin and LY294002) dose-dependently inhibited TPO-induced aggregation of CML-CP platelets. The Abl kinase inhibitor imatinib mesylate and the Jak inhibitor AG490 suppressed TPO-induced aggregation of CML-CP platelets. Pretreatment with imatinib mesylate, but not with AG490, inhibited the activity of PI3-kinase in CML-CP platelets. In addition, tyrosine phosphorylation of Jak2 was undetected in CML-CP platelets before TPO treatment. These findings indicate that the constitutive activation of PI3-kinase primes CML-CP platelets for the aggregation induced by TPO, and that Bcr-Abl, but not Jak family protein tyrosine kinases, are involved in the constitutive activation of PI3-kinase in CML-CP platelets.
    Preview · Article · Jul 2004 · Leukemia
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    ABSTRACT: Signal transducers and activators of transcription (STAT) proteins are transcription factors activated by phosphorylation on tyrosine residues after cytokine stimulation. In erythropoietin receptor (EPOR)-mediated signaling, STAT5 is tyrosine-phosphorylated by EPO stimulation. Although Janus Kinase 2 (JAK2) is reported to play a crucial role in EPO-induced activation of STAT5, it is unclear whether JAK2 alone can tyrosine-phosphorylate STAT5 after EPO stimulation. Several studies indicate that STAT activation is caused by members of other families of protein tyrosine kinases such as the Src family. We previously reported that reduction of Src by induction of antisense src RNA expression suppressed EPO-promoted erythroid differentiation in K562 cells. In the present study, we explored the function of Src downstream of the EPOR-initiated signaling. Reduction of Src diminished tyrosine phosphorylation of STAT5 in K562 cells regardless of EPO treatment. The tyrosine phosphorylation level of STAT5 induced by EPO in F-36P cells was reduced in the presence of PP1 or PP2 selective Src inhibitor. In addition, the expression of dominant negative Src in F-36P cells reduced the tyrosine phosphorylation of STAT5. When Src and STAT5 were co-expressed in COS7 cells, tyrosine phosphorylation of STAT5 was observed, and tyrosine residue 694 (Tyr 694) of STAT5A was identified as the major phosphorylation site by Src. In vitro kinase assay revealed that GST-STAT5 fusion protein with the conserved C-terminal, but not the C-terminal-truncated mutant which lacks Tyr 694, was tyrosine-phosphorylated by Src. Src can thus directly tyrosine-phosphorylate the activation site of STAT5 (Tyr 694 in STAT5A), and Src may contribute to EPO-induced signal transduction via STAT5.
    Preview · Article · Nov 2001 · Oncogene
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    ABSTRACT: In this study, we examined the molecular mechanism of erythropoietin-initiated signal transduction of erythroid differentiation through Src and phosphatidylinositol 3-kinase (PI3-kinase). Antisense oligonucleotides against src but not lyn inhibited the formation of erythropoietin-dependent colonies derived from human bone marrow cells and erythropoietin-induced differentiation of K562 human erythroleukaemia cells. Antisense p85alpha oligonucleotide or LY294002, a selective inhibitor of PI3-kinase, independently inhibited the formation of erythropoietin-dependent colonies. In K562 cells, Src associated with PI3-kinase in response to erythropoietin. Antisense src RNA expression in K562 cells inhibited the erythropoietin-induced activation of PI3-kinase and its association with erythropoietin receptor. PP1, a selective inhibitor of the Src family, reduced erythropoietin-induced tyrosine phosphorylation of erythropoietin receptor and its association with PI3-kinase in F-36P human erythroleukaemia cells. The coexpression experiments and in vitro kinase assay further demonstrated that Src directly tyrosine-phosphorylated erythropoietin receptor, and associated with PI3-kinase. In vitro binding experiments proved that glutathione S-transferase-p85alpha N- or C-terminal SH2 domains independently bound to erythropoietin receptor, which was tyrosine-phosphorylated by Src. Taken together, Src transduces the erythropoietin-induced erythroid differentiation signals by regulating PI3-kinase activity.
    Preview · Article · Nov 2001 · The EMBO Journal
  • K Okamoto · H Kamano · I Sakata

    No preview · Article · Dec 2000 · Transplantation Proceedings
  • H Kamano · K Okamoto · I Sakata · Y Kubota · T Tanaka

    No preview · Article · Dec 2000 · Transplantation Proceedings
  • K. Okamoto · H. Kamano · I. Sakata
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    ABSTRACT: Light irradiation onto leukemia cells by LED were performed at 37 °C using several sets of BIOLED placed in a CO<sub>2</sub> incubator under a fixed light intensity. We investigated the effect of light wavelength on the cell division. Irradiations onto cells were done for two kind of culture media: (1) with an addition of metal porphyrin which is used in photodynamic therapy (PDT) of cancers, and (2) without any additive
    No preview · Conference Paper · Feb 1999
  • H. Kamano · K. Okamoto · I. Sakata · Y. Kubota · T. Tanaka
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    ABSTRACT: We applied a high-brightness light emitting diode (LED) and gallium-metal porphyrin (Ga-C10-P) for leukemia cell purging. The blue, blue-green, green, yellow-green, yellow, orange and red color LEDs were constructed in photo-irradiation equipment (BIOLED). We set up the BIOLED in a CO<sub>2</sub> incubator and cultured human chronic myelocytic leukemia cell line K562 in RPMI1640. We irradiated 525 nm green light, 612 nm orange light or 660 nm red fight on to the K562 cells in the presence of 0.1 μM or 1 μM Ga-C10-P. Surprisingly, the 525 nm photo-irradiation induced the suppression of proliferation of leukemia cells in the presence of 1 μM Ga-C10-P. Our results suggest a possibility of eliminating leukemia cells in autologous bone marrow transplantation by photodynamic purging using LED light and Ga-C1O-P
    No preview · Conference Paper · Feb 1999
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    ABSTRACT: We constructed a recombinant plasmid which expresses antisense src RNA after dexamethasone (Dexa) treatment, and transfected it into U937 human monoblastic leukemia cells (U937-ASRC). Induction of antisense src RNA expression diminished the amounts of c-Src and its protein tyrosine kinase (PTK) activity in U937-ASRC cells. The declines in c-Src and its PTK activity subsequently reduced the proliferation of U937-ASRC cells. To elucidate the growth signal transduction pathway downstream of c-Src, tyrosine phosphorylation of Shc was examined in U937-ASRC cells treated with Dexa. The decline in c-Src by induction of antisense src RNA expression decreased the level of tyrosine phosphorylation of Shc. Immunoprecipitated c-Src directly phosphorylated immunoprecipitated Shc on tyrosine residues in vitro. The amounts of Grb2 and Sos co-immunoprecipitated with Shc were decreased after Dexa treatment. However, the amount of Sos co-immunoprecipitated with Grb2 was apparently not affected by Dexa treatment. These results indicate that Grb2 and Sos constitutively associate with each other in U937 cells. Furthermore, the level of phosphorylation on tyrosine (204) essential for MAP kinase activation was decreased after Dexa treatment. Taken together with all these findings, it is suggested that c-Src directly phosphorylates Shc on tyrosine residues, which in turn binds to Grb2 constitutively associated with Sos to form a Shc-Grb2-Sos complex, and that the complex formation is coupled with MAP kinase activation mediated by Ras activation in U937 cells.
    Preview · Article · May 1997 · Leukemia
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    ABSTRACT: In order to elucidate the role of c-myb gene in erythroid differentiation of K562 cell induced by hemin (Hm) and erythropoietin (Epo), we constructed recombinant plasmid that could produce antisense myb RNA after induction with dexamethasone. During treatment with Hm, K562 cells constitutively expressed c-myb mRNA, and 50% of them began to synthesize hemoglobin (Hb). Expression of antisense myb RNA reduced the amount of c-myb mRNA, and the percentage of Hb-synthesizing cells was decreased to 20%. In the presence of Epo, c-myb mRNA declined and 20% of K562 cells synthesized Hb regardless of antisense myb RNA expression. It is suggested that constitutive expression of c-myb mRNA is necessary for Hm-induced differentiation, and that a decrease in the amount of c-myb mRNA induced by antisense myb RNA expression suppresses Hm-induced differentiation. The amount of c-myb mRNA in K562 cells was reduced during the differentiation induced by Epo. Expression of GATA-1 mRNA was almost constant during Hm-induced differentiation, but increased during Epo treatment. It is supposed that the mechanism of Hm-induced differentiation is distinguished from that of Epo-induced differentiation in K562 cells.
    No preview · Article · Sep 1994 · Biochemistry and molecular biology international
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    ABSTRACT: To elucidate the role of pp60c-src in U937 human monoblastoid leukemia cell proliferation, recombinant plasmids containing the src gene or myb gene, which could produce antisense src or antisense myb RNA after dexamethasone treatment, were constructed and transfected into U937 cells (U937-ASRC, U937-AMYB). pp60c-src synthesis in U937-ASRC was diminished by the third day after induction of antisense src RNA and the cell proliferation was reduced, furthermore, the amount of p75c-myb was significantly decreased by the third day. p75c-myb synthesis in U937-AMYB was diminished by the second day after induction of antisense myb RNA and the cell growth was significantly inhibited but the amount of pp60c-src in U937-AMYB was not reduced. These results suggest that a decrease in the amount of pp60c-src leads to an inhibition of p75c-myb expression and subsequent reduction in the U937 cell proliferation.
    No preview · Article · Jul 1994 · Biochemical and Biophysical Research Communications
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    ABSTRACT: We constructed a recombinant plasmid which expresses antisense src RNA in human cells and used it as a tool for investigating the role of pp60c-src in proliferation and differentiation of K562 human leukemia cells. In erythropoietin (EPO)-responsive cells, EPO induces rapid tyrosine phosphorylation of several cellular proteins including EPO receptor (EPOR) although EPOR has no tyrosine kinase domain. Here we show that antisense src RNA expression suppresses pp60c-src synthesis in the recombinant plasmid-transfected K562 cells, reduces the proliferation and inhibits hemoglobin synthesis and glycophorin A expression promoted by EPO in K562 cells. These findings suggest that pp60c-src plays crucial roles in the proliferation and EPO-induced erythroid differentiation of K562 cells.
    No preview · Article · Jul 1994 · Biochemical and Biophysical Research Communications
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    ABSTRACT: Inosine monophosphate dehydrogenase (IMPDH, EC 1.1.1.205) inhibitors including mycophenolic acid (MPA) were reported to induce K562 human leukemia cells to differentiate into erythroid cells. A shuttle vector plasmid pMSG containing E. coli xanthine-guanine phosphoribosyl transferase (Eco gpt) gene was transfected into K562 cells (K562-pMSG cells) to investigate the role of IMPDH in both K562 cell proliferation and erythroid differentiation. Eco gpt provides K562 cells with an additional salvage pathway for GMP production from xanthine. In the presence of xanthine, K562-pMSG cells continued to proliferate and did not differentiate to erythroid cells regardless of the presence of MPA, but they discontinued proliferating and differentiated into the erythroid lineage in the absence of xanthine. Proliferation and differentiation of control K562 cells into erythroid cells were suppressed by MPA regardless of the presence or absence of xanthine. Addition of guanosine maintained the proliferation and blocked the erythroid differentiation of K562 and K562-pMSG cells induced by MPA even in the absence of xanthine. These data indicate that a decrease in the activity of IMPDH and a subsequent decline in the concentration of guanine nucleotides caused by MPA resulted in the induction of the erythroid differentiation and discontinuation of the proliferation of K562 cells.
    No preview · Article · Apr 1992 · Biochemistry international
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    ABSTRACT: The effects of 4-carbamoylimidazolium 5-olate (SM-108), an antipurine compound, on a human leukemia cell line, K562, were studied. Treatment with SM-108 induced erythroid differentiation of K562 cells. During a 6-day culture with 100 microM SM-108, the cell number decreased to 37% of the control number, 77% of the cells became benzidine-positive, and the hemoglobin content increased from 2.1 +/- 0.2 to 10.6 +/- 1.3 pg/cell. Cell differentiation was associated with reduction of IMP dehydrogenase activity and intracellular GTP content to 25 and 36%, respectively, of the control values within 1.5 hr. The differentiation and decrease in the GTP pool induced by SM-108 were blocked by the presence of 25 microM guanine or guanosine. SM-108 also induced erythroid differentiation of K562 subline cells transfected with pMSG (K562/pMSG), which have an additional salvage pathway for GMP production from xanthine. The addition of 100 microM xanthine prevented erythroid differentiation of this subline and restored the GTP pool. These findings suggest that the induction of erythroid differentiation of K562 cells by SM-108 may be due to an early decrease in IMP dehydrogenase activity and a subsequent decrease in GTP content in the cells. Thus, purine metabolism may have an important role in SM-108-induced differentiation.
    No preview · Article · Dec 1991 · Biochemical Pharmacology
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    ABSTRACT: Recombinant plasmids containing v-myb' (803 bp fragment of the 3' end of v-myb) were constructed to induce sense or antisense v-myb' RNA expression with dexamethasone in human cells. These plasmids were used as a tool for the investigation of the role of c-myb gene in human leukemia cell proliferation and differentiation. They were transfected by electroporation into the K562 human leukemia cell line derived from a patient with chronic myelogenous leukemia in blastic crisis. After induction of transcription by dexamethasone, the plasmid with antisense v-myb' repressed the expression of p75c-myb from the endogenous c-myb gene of K562 cells. It also reduced the proliferation rate of K562 cells to 50% of the control level, and induced these K562 cells to express the myelomonocytic differentiation cell surface marker CD13 and increased NBT reducing activity. The plasmid with sense v-myb' did not have an effect on p75c-myb expression, the proliferation of K562 cells or the expression of myelomonocytic differentiation phenotypes. These observations suggest that antisense v-myb' RNA represses p75c-myb expression and that a decrease of p75c-myb suppresses K562 cell proliferation and induces its differentiation towards the myelomonocytic lineage.
    No preview · Article · Feb 1990 · Leukemia Research

Publication Stats

276 Citations
58.79 Total Impact Points

Institutions

  • 2000-2013
    • Kagawa University
      • • Graduate School of Engineering
      • • Faculty of Medicine
      Takamatu, Kagawa, Japan
  • 1988
    • Nagai Internal Medicine Clinic
      Okayama, Okayama, Japan