Emilie Dussossoy

Université de Montpellier, Montpelhièr, Languedoc-Roussillon, France

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Publications (7)10.56 Total impact

  • E. Bony · E. Dussossoy · A. Michel · F. Boudard · J. Giaimis · P. Brat · N. Barouh · G. Piombo
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    ABSTRACT: Awara is a palm tree common in the French Guyana, used for the nutritional properties of the fruit and in the traditional pharmacopoeia of the country. The pulp of this fruit is rich in oil of which the unsaponifiable fraction contains a lot of antioxidant and anti-inflammatory molecules. The aim of this study was to characterize the chemical composition of the unsaponifiable fraction of awara pulp oil and to evaluate the in vitro anti-inflammatory activities. In a first part, chemical analyses of the unsaponifiable fraction allowed us to determined for 1 mg of unsaponifiable fraction: 6.8 μg of α-tocopherol, 125.7 μg of carotenoids (mainly β-carotene), and 152.6 μg of phytosterols (mainly β-sitosterol). In a second part, the unsaponifiable fraction has shown a significant inhibition of the cyclooxygenase 1 and 2 activities (COX-1 and -2 respectively). On LPS/IFNγ-stimulated J774 macrophages, it strongly inhibited the PGE2 production in a dose dependent manner. The unsaponifiable fraction produced a decrease of an important proinflammatory mediator, which might be due by the inhibition of the corresponding enzyme activity showing potential anti-inflammatory properties. These effects could be explained by the presence of antioxidant molecules like tocopherols and carotenoids, and anti-inflammatory molecules like sterols.
    No preview · Article · Jun 2014 · Acta horticulturae
  • E. Dussossoy · E. Bony · A. Michel · P. Brat · F. Vaillant · F. Boudard · J. Giaimis
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    ABSTRACT: The noni fruit (Morinda citrifolia L.) juice is used since more than 2000 years in Polynesia and South-America in traditional medicine. The market of this fruit is continuously increasing despite no clear evidence of its biological activity being established. The aim of this study was therefore to evaluate the anti-oxidative and anti-inflammatory activities of a noni juice from Costa Rica. Total phenolic compounds and ascorbic acid were determined by an optimized Folin-Ciocalteu method. The anti-oxidative activity of noni juice was measured in vitro by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. The antiinflammatory effect was investigated in vitro (cyclooxygenases inhibition) and in rats on a carrageenan-induced paw oedema model, after oral or intraperitoneal administration. The total phenolic content and ascorbic acid contents of noni juice were 476 and 753 mg/L respectively. Noni juice had a medium antioxidant activity, inhibited cyclooxygenases and significantly reduced carrageenan-induced paw oedema. This study showed that noni juice has anti-inflammatory and anti-oxidative effects.
    No preview · Article · Jun 2014 · Acta horticulturae
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    ABSTRACT: Awara (Astrocaryum vulgare M.) pulp oil has been shown to possess anti-inflammatory properties in vivo, and contains an unsaponifiable matter rich in bioactive compounds. This study focused on the ethanolic unsaponifiable fraction (EUF) of awara pulp oil. Its chemical composition has been characterized: carotenoid, phytosterol, and tocopherol contents represent 125.7, 152.6, and 6.8 μg/mg of EUF, respectively. We further evaluated this fraction for anti-inflammatory properties in J774 macrophages activated by lipopolysaccharide (LPS) plus interferon (IFN) γ to understand the biological effects of awara pulp oil. EUF strongly decreased nitric oxide (NO), prostaglandin E(2), tumour necrosis factor (TNF) α, and interleukin (IL) -6 and -10 production in activated J774 cells. Moreover, it inhibited expression of inducible NO synthase and cyclooxygenases-2 in vitro. The anti-inflammatory properties of EUF were also confirmed in vivo by modulation of TNFα, IL-6 and IL-10 serum concentration in an endotoxic shock model. Pre-treatment with awara oil fraction offers promise as a protective means to lower the production of excessive amounts of pro-inflammatory molecules.
    Full-text · Article · Nov 2012 · Plant Foods for Human Nutrition
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    ABSTRACT: Nurses endure daily low-level exposure to cytotoxic drugs, which can lead to significant absorption with potential harmful consequences. New sterile medical devices called cytotoxic safe infusion systems (CSISs), intended by their manufacturers to improve safety and quality of cytotoxic drug infusions, have been made commercially available. CSISs from 3 manufacturers were tested in 2 cancer units and compared with standard infusion sets. The aim of this study is to evaluate the devices regarding occupational exposure, quality of the infusion, and economic aspects.
    No preview · Article · Sep 2012 · Journal of infusion nursing: the official publication of the Infusion Nurses Society
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    ABSTRACT: Awara (Astrocaryum vulgare M.) is a palm fruit mainly used in nutrition. We analysed the pulp oil for fatty acid, tocopherol, carotenoid, and phytosterol and we evaluated whether this oil may attenuate inflammation in vivo. In an endotoxic shock model, awara pulp oil treatment decreased pro-inflammatory cytokines and increased anti-inflammatory cytokines. In a pulmonary inflammation model, awara pulp oil treatment reduced eosinophil and lymphocyte numbers recovered into the broncho-alveolar lavages. These results suggest that awara pulp oil administration can efficiently counteract an acute and chronic inflammatory response in vivo that is probably mediated by fatty acids and minor compounds.
    Full-text · Article · Sep 2011 · Fitoterapia

  • No preview · Article · Mar 2011 · Retina (Philadelphia, Pa.)
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    E Dussossoy · P Brat · E Bony · F Boudard · P Poucheret · C Mertz · J Giaimis · A Michel
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    ABSTRACT: Noni fruit (Morinda citrifolia L.) juice has been used for more than 2000 years in Polynesia as a traditional folk medicine. The aim of the present study was to finely characterize noni juice from Costa Rica and to evaluate its anti-oxidative and anti-inflammatory activities. A microfiltrated noni juice was prepared with Costarican nonis. HPLC-DAD and Electro Spray Ionization Mass Spectrometric detection (HPLC-ESI-MS) were used to identify phenolic compounds and iridoids. The anti-oxidative activity of noni juice was measured in vitro by both Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging methods. The anti-inflammatory effects of noni juice were investigated in vitro by: measuring its effect on nitric oxide and prostaglandin E2 production by activated macrophages, evaluating its inhibitory activities on cyclooxygenase (COX)-1 and -2 and in vivo on a carrageenan-induced paw oedema model in rats. Several polyphenols belonging to the coumarin, flavonoid and phenolic acid groups, and two iridoids were identified. Noni juice demonstrated a mean range free radical scavenging capacity. Furthermore, it also reduced carrageenan-induced paw oedema, directly inhibited cyclooxygenase COX-1 and COX-2 activities and inhibited the production of nitric oxide (NO) and prostaglandins E(2) (PGE(2)) in activated J774 cells, in a dose dependent manner. This study showed that noni's biological effects include: (1) anti-oxidant properties probably associated with phenolic compounds, iridoids and ascorbic acid and (2) anti-inflammatory action through NO and PGE(2) pathways that might also be strengthened by anti-oxidant effects.
    Full-text · Article · Jan 2011 · Journal of ethnopharmacology