[Show abstract][Hide abstract] ABSTRACT: The plant Plumbago zeylanica Linn. of Plumbaginaceae known as Chitraka/ Chitramoolam is a popular drug in Ayurveda and Siddha. The root of this plant finds place in several of the compound formulations of these systems of medicine, as one of the ingredients. The root being a vital component in these formulations, its quality and consistency is of prime importance. Plumbagin being the major bioactive chemical was used as a reference standard with which the HPTLC chromatogram obtained for each sample was compared. Plumbagin present in each sample was quantified using a calibration graph of the reference standard. The present study deals with the study of the presence of any variation in the plumbagin content due to change in season and storage. Sufficient quantities of root was collected during the month of July 2008 and stored at room temperature for the assay of Plumbagin. From August onwards every month, fresh root samples were also collected till June 2009. Simultaneous comparison of the fingerprints of n-hexane extract of the roots as well as estimation of plumbagin was done on the samples collected and the stored sample at room temperature using HPTLC technique. The plumbagin content showed a sharp decline from July 2008 to June 2009 on the stored root samples. The study established more concentration of Plumbagin constituent in February followed by January. The content was found to be very less in April. Hence the root may show more efficacy if collected in February. The technique is simple and cost effective.
No preview · Article · Apr 2011 · International Journal of Pharmacy and Technology
[Show abstract][Hide abstract] ABSTRACT: Root bark of Alangium salvifolium (Linn.f.) Wang. (Family Alangiaceae) is a reputed drug mentioned in the ancient books of Ayurveda and Siddha for the treatment of epilepsy, jaundice, hepatitis etc. Root bark of the plant was subjected to macro-microscopic, photomicrographic, physico-chemical, fluorescence, preliminary phytochemical, TLC and HPTLC to fix quality standards for this drug. Microscopic studies have shown stratified phellem, rhytidome, cluster crystals of calcium oxalate and uni- to triseriate medullary rays in the root bark. Chloroform, ethyl acetate, ethanol extracts and alkaloid fraction revealed characteristic chromatographic patterns with presence of alkaloids in varying concentrations. This study would be useful in the identification and authentication of the raw drug.
Full-text · Article · Aug 2010 · Pharmacognosy Journal
[Show abstract][Hide abstract] ABSTRACT: Root of Cocos nucifera Linn. (Arecaceae) is astringent, diuretic, anthelmintic and useful in curing pharyngodynia, uterine disorders, blenorrhagia, bronchitis, hepatopathy, strangury and helminthiasis. It is an important commodity in folklore medicines of south India for urinary troubles. So far there is no record on detailed pharmacognostic evaluation of this crude drug, hence this communication throws light on parameters essential to fix standards for this medicinal material.
Full-text · Article · Aug 2010 · Pharmacognosy Journal
[Show abstract][Hide abstract] ABSTRACT: Two flavonoids and two carboxylic acids have been isolated from the ethyl acetate extract of the roots. The structures of these compounds have been established as myricetin-3,3',5',7-tetra methyl ether 1. ampelopsin-3',4',5',7-tetramethyl ether 2, plumbagic acid 3 and roseanoic acid 4 on the basis of UV, IR, H-1 and C-13 NMR and mass spectrum studies. The two carboxylic acids 3 and 4 arc reported here for the first time. Compounds 1 and 2 are the first report from the genus plumbago.
[Show abstract][Hide abstract] ABSTRACT: Two flavonoids and two carboxylic acids have been isolated from the ethyl acetate extract of the roots. The structures of these compounds have been established as myricetin-3,3′,5′,7-tetra methyl ether 1, ampelopsin-3′, 4′, 5′,7-tetramethyl ether 2, plumbagic acid 3 and roseanoic acid 4 on the basis of UV, IR, 1H and 13 C NMR and mass spectrum studies. The two carboxylic acids 3 and 4 are reported here for the first time. Compounds 1 and 2 are the first report from the genus plumbago.
[Show abstract][Hide abstract] ABSTRACT: Physicochemical parameters of roots of three Plumbago species, Plumbago capensis, P. rosea and P. zeylanica belonging to Plumbaginaceae were analyzed. Microbial contamination, aflatoxins, pesticide residue and heavy metal content were also determined. Attempt has also been made to estimate the biologically active chemical plumbagin present in them and the data compared. The study ensures that the quality control parameters do help in the proper standardization of the crude drugs in drug development process for global acceptance.
No preview · Article · Jan 2010 · Indian Journal of Pharmaceutical Sciences
[Show abstract][Hide abstract] ABSTRACT: Bark of the species of Ficus namely F. bengalensis Linn., F. racemosa Linn. and F. religiosa Linn. (Moraceae) are reported to be medicinally useful. The authentic samples of stem bark of the three species were subjected to HPTLC analysis. Heavy metal content such as lead, cadmium, mercury and arsenic and minerals like iron, copper, manganese, zinc, nickel, cobalt, chromium analysis using atomic absorption spectroscopy and pesticide residues by GC-MS were carried out. Study revealed that heavy metals are within the permissible limit and organochlorine pesticide residues are not detected in the three species. The data evolved in the present work will aid in identifying these drugs in dry form and in standardization of the drug.
[Show abstract][Hide abstract] ABSTRACT: The present communication attempts to evaluate the pharmacognostical and physicochemical parameters of the roots of Plumbago capensis Thunb.; (syn. Plumbago auriculata Lam.) fam. Plumbaginaceae, a sub-scandent shrub with diffuse branches, oblong or oblong-spathulate leaves with pale blue flower. It is indigenous to South Africa and grown in the gardens of India as an ornamental plant. The roots are used as styptic, scrofula, emetic, smeared to remove warts, headache, decoction is used in black water fever. As there is no pharmacognostic work reported on root, the macro-and microscopic characters are carried out and demonstrated along with their physicochemical parameters and fluorescence analysis. The root shows thick walled lignified groups of pericyclic fibres, xylem region and medullary ray cells consists of simple starch grains microscopically. Physicochemical studies revealed, total moisture content (8.11%), total ash (2.62%), acid insoluble ash (0.68%), water-soluble ash (1.89%), alcohol soluble extractive value (9.14%), and water-soluble extractive value (13.18%). Preliminary organic analysis revealed the presence of quinone, flavones, steroid, phenol in difference extracts respectively. These specific identities will be useful in identification of the raw drug in dried form.