[Show abstract][Hide abstract] ABSTRACT: Staphylococcus aureus is a major human pathogen, implicated in both community and hospital acquired infections. The therapy for methicillin-resistant Staphylococcus aureus (MRSA) infections is becoming more difficult because of multidrug resistance and strong biofilm-forming properties. Schiff bases have attracted attention as promising antibacterial agents. In this study, we investigated the in vitro activity of taurine-5-bro-mosalicylaldehyde Schiff base(TBSSB) against MRSA. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) were determined using a microtitre broth dilution method. TBSSB effectively inhibited planktonic MRSA,with MIC of 32μg/mL. The time-kill curve confirmed that TBSSB exhibited bactericidal activity against MRSA. TBSSB was also found to significantly inhibit MRSA biofilm formation at 24h, especially at 1×MIC and sub-MIC levels. Furthermore, Scanning ele￢ctron microscopy (SEM) and transmission electron microscopy (TEM) showed remarka￢ble morphological and ultrastructural changes on MRSA cell surface due to exposure to TBSSB. This study indicated that TBSSB may be an effective bactericidal agent against MRSA.
Preview · Article · Apr 2014 · Journal of Microbiology and Biotechnology
[Show abstract][Hide abstract] ABSTRACT: As the traditional Chinese medicine, the fresh fruits of Amorpha fruticosa L. were applied for the treatment of carbuncle, eczema and burn (Das et al., 2007). However, little is known about the functional roles of the fruits of Amorpha fruticosa L. during wound healing progress. In the present study, we evaluated both antimicrobial potential against a wide range of microorganisms and wound healing activity of the seven compounds isolated from the fruits of Amorpha fruticosa L in vitro and in vivo. Our results showed that compounds I (6a,12a-dehydroamorphin), V (dehydrosermundone) and VI (tephrosin) isolated from the fruits of Amorpha fruticosa L. performed dominant antimicrobial potential against microorganisms. Moreover, these compounds significantly enhanced fibroblasts proliferation and migration, leading to promotion of wound healing. Thus, it could be possible for the therapeutic utilization of Amorpha fruticosa L. for wound healing in the future.
No preview · Article · May 2013 · African Journal of Traditional, Complementary and Alternative Medicines
[Show abstract][Hide abstract] ABSTRACT: Tannins extracted from immature fruits of Terminalia chebula Fructus Retz. are considered as effective components promoting the process of wound healing. The objective of this study is to explore the optimal extraction and purification technology (OEPT) of tannins, while studying the use of this drug in the treatment of a cutaneous wound of rat as well as its antibacterial effects.
The content of tannin extracts was measured by the casein method, and antibacterial ability was studied by the micro-dilution method in vitro. In wound healing experiment, animals in group Ⅰ, Ⅱ and Ⅲ were treated with vaseline ointment, tannin extracts (tannin content: 81%) and erythromycin ointment, respectively (5 mg of ointment were applied on each wound). To evaluate the process of wound healing, selected pharmacological and biochemical parameters were applied.
After optimal extraction and purification, content of tannin extracts was increased to 81%. Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro. After excision of wounds, on days 7 and 10, the percent of wound contraction of group Ⅱ was higher than that of group Ⅰ. After being hurt with wounds, on days 3, 7, and 10, the wound healing quality of group Ⅱ was found to be better than that of group Ⅰ in terms of granulation formation and collagen organization. After wound creation, on day 3, the vascular endothelial growth factor expression of group Ⅱ was higher than that of group Ⅰ.
The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.
Preview · Article · Oct 2011 · BMC Complementary and Alternative Medicine
[Show abstract][Hide abstract] ABSTRACT: Objective) To study the relationship between plasma endotoxin and renal function in acute renal failure induced by high dose cisplatin (DDP) in rats. (Method) 36 SD rats were divided randomly into 6 groups: DDP 6 h, DDP 48 h, DDP control, normal saline (NS) 6 h, 48 h and NS control group ( n = 6 in each group). 10 mg/kg DDP was injected intraperitoneally while same volume of natural saline was given as the control. Toxic effects of DDP were observed subsequently. Heparinized blood samples were obtained by heart punctures 6 h and 48 h after DDP and NS administration and plasma endotoxin was measured by using the BET-16 bacterium endotoxin cryoscope. At the same time blood samples were obtained from orbital venous sinus and blood urea nitrogen (BUN) and creatinine (Cre) were measured by automatic biochemistry analyzer. (Result) Body weight of the rats decreased significantly at 6 h after DDP administration; diarrhea increased progressively at 48 h after DDP administration and the rats died in 3 d. There were no significant differences in blood BUN and creatinine between cisplatin group and control group 6 hours after administrations (P >0.05); Blood BUN and Cre increased to (18.71 ±9.9) mmol/L and (49.6±14.1) μmol/L respectively in rats 48 h after DDP administrations, significantly higher than the (7.48 ±0.6) mmol/L and (27.17±1.7) μmol/L in control group (P
[Show abstract][Hide abstract] ABSTRACT: Currently, a pandemic swine influenza A virus infection causes a huge negative impact on human beings all over the world. However, the methods of treatment are not satisfactory, therefore it is urgent for us to set up new theory and practice to fight against the intractable virus. Terminalia chebula Retz, as a kind of traditional Chinese medicine, widely distributes and has multiple pharmacological effects. Evidences in laboratory and clinic practice confirm us to the potential of Terminalia chebula Retz inhibiting influenza A virus infection. We thus hypothesize that acetone extracts (tannic acids, A) of Terminalia chebula Ret may as a new alternative treat influenza A infection based on holistic concept of traditional Chinese medicine principle.
Preview · Article · Apr 2010 · African journal of microbiology research
[Show abstract][Hide abstract] ABSTRACT: fatty acids are considered to be effective components to promote wound healing and Lucilia sericata larvae are applied clinically to treat intractable wounds. We aimed to investigate the effect of fatty acid extracts from dried Lucilia sericata larvae on murine cutaneous wound healing as well as angiogenesis.
On day 7 and 10 after murine acute excision wounds creation, the percent wound contraction of fatty acid extracts group was higher than that of vaseline group. On day 3, 7 and 10 after wounds creation, the wound healing quality of fatty acid extracts group was better than that of vaseline group on terms of granulation formation and collagen organization. On day 3 after wounds creation, the micro vessel density and vascular endothelial growth factor expression of fatty acid extracts group were higher than that of vaseline group. Component analysis of the fatty acid extracts by gas chromatography-mass spectrometry showed there were 10 kinds of fatty acids in total and the ratio of saturated fatty acid, monounsaturated fatty acid and polyunsaturated fatty acid (PUFA) was: 20.57%:60.32%:19.11%.
Fatty acid extracts from dried Lucilia sericata larvae, four fifths of which are unsaturated fatty acids, can promote murine cutaneous wound healing probably resulting from the powerful angiogenic activity of the extracts.
Full-text · Article · Mar 2010 · Lipids in Health and Disease
[Show abstract][Hide abstract] ABSTRACT: Human has used maggot to treat diseases for thousands of years. In recent years, with abuse of antibiotic and the rising incidence of antibiotic resistance, maggot therapy, as a surgical alternative, is mainly applied to treat chronic infected wounds on account of its low cost, efficacy and safety. Its mechanisms are disinfection, bio-debridement and enhancement of tissue regeneration. Maggot therapy which serves as a kind of biological therapy is promising. However, living maggot therapy could result in inevitable complications, so that we should apply traditional Chinese medicine theory to investigate and develop new delivery method of maggot. The review summarizes the past and present of maggot therapy.
No preview · Article · Dec 2009 · Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
[Show abstract][Hide abstract] ABSTRACT: Taurine plays various important roles in a large number of physiological and pathological conditions in human body, such as the cytoprotective functions, antioxidant, anti-inflammatory and anti-apoptosis effects. Copper demonstrates a critical effect in the processes of wound healing, including induction of endothelial growth factor, angiogenesis, antimicrobial potency and expression and stabilization of extracellular matrix. Both copper and taurine are effective in accelerating wound healing, but it was rarely reported about the formation of copper complexes of taurine and the effect of the compound in wound healing. Generally speaking, to human body, organic compound could provide a better bioavailability than the inorganic ones. We thus hypothesize that taurine combined with copper would be a new therapeutic candidate for infected wound healing. We name the new compound copper-taurine (CT). Copper-taurine (CT) added into the wound dressings would not only reduce the risk of wound infection, but, more importantly, would stimulate wound repair directly. The sustained release of copper and taurine from the wound dressings into the wound site would together facilitate the wound healing more potently.
No preview · Article · Aug 2009 · Medical Hypotheses
[Show abstract][Hide abstract] ABSTRACT: Several kinds of resins were investigated in the first step and D101 macroporous resin was selected for cleaning-up naringin
(NAR), a major flavonoid glycoside from Fructus aurantii. In the subsequent column chromatography, 10% aqueous ethanol was first used to elute the column to remove the undesired
constituents and 70% aqueous ethanol was used to elute the target. The content of NAR was 57.1% with 95.7% recovery in this
process. In the second step, the obtained crude sample was directly isolated by high-speed counter-current chromatography
(HSCCC) with a two-phase solvent system composed of ethyl acetate–n-butanol–water at a volume ratio of 2: 0.8: 3.2 (v/v/v), and 331mg NAR with 98.3% purity was obtained from 600mg crude extract in only one run. The recovery of the compound in
this step was 95.0%. Thus, the total recovery of NAR was 90.9% after the two step purification. The established protocol for
large-scale isolation and separation of NAR with high purity and recovery from F. aurantii was simple, efficient, and suitable for pharmace- utical and commercial use.