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ABSTRACT: To construct of HESR1 siRNA plasmid expression vector. Cultures human umbilicus vein endothelial cell, according to the computer aided design, HESR1-specific siRNA gene was synthesized and cloned into the RNAi-Ready pSIREN-RetroQ ZsGreen Vector. The constructed HESR1-RNAi plasmid were transfected into human umbilicus vein endothelial cell, RT-PCR detected the effected of HESR1-RNAi plasmid. The recombinant was cloned and the sequence was obtained, HESR1-RNAi plasmid can down-regulated the expression of HESR1 in human umbilicus vein endothelial cell. Conclusion : Successfully cloning the recombinant make it possible searching new mechanism of HESRI in angiogenesis.
Sun Yat-Sen University
Guangzhou, Guangdong Sheng, China
- State Key Laboratory of Oncology