[Show abstract][Hide abstract] ABSTRACT: Filaments were induced from cut edges of segments of Gloiosiphonia capillaris, Gloiopeltis furcata, Chondrus ocellatus, Solieria tenuis, Ceramium kondoi, Chondria tenuissima, Laurencia okamurai and from the intact surfaces of Ceramium kondoi and Chondria tenuissima under unialgal culture conditions. The filaments of Gloiosiphonia capillaris can grow vegetatively under free-living conditions, while filaments of Gloiopeltis furcata tended to differentiate after excision from explants and grew autotrophically to the 10- to 20-cell stage. Filaments of other species usually survived attached to generating explants. Two distinct modes of filament regeneration were discerned among the species. In Gloiosiphonia capillaris, Gloiopeltis furcata, Chondrus ocellatus and Solieria tenuis, filaments initially developed into crust or crust-like compact tissue in suspension culture; upright thalli developed from the crusts. In Ceramium kondoi, Chondria tenuissima and Laurencia okamurai, thalli were generated from pseudoparenchyma that was developed from filaments, while creeping parts (rhizoids) formed a prostrate system.
[Show abstract][Hide abstract] ABSTRACT: Chrysochromulina planisquama sp. nov. is described from the cultures originating from water samples collected in Jiaozhou Bay, Yellow Sea. Cells are spherical to subspherical with a diameter of 3.0-6.0 mu m. They have two unequal flagella and a haptonema, which is shorter than the flagella. Two types of scales form multiple layers on the cell surface. Both types of scales are flat and circular. The larger scales measure 0.70-0.85 mu m in diameter and are ornamented with a pattern of 52-60 radiating ribs on the proximal surface and faint concentric rings on the distal surface. The smaller scales measure 0.38-0.45 mu m in diameter. They have a similar pattern but with fewer radiating ribs (ca. 28-30) on the proximal surface. Each cell contains two chloroplasts, a nucleus and two mitochondria. Scales are produced in the Golgi apparatus.