Chen Shuang

Harbin Medical University, Charbin, Heilongjiang Sheng, China

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Publications (4)10.71 Total impact

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    ABSTRACT: To explore the interactions among immunity related genes and the risk of breast cancer (BC), 376 invasive ductal carcinoma (IDC) of the breast cases and 366 healthy controls were selected into our study. Twenty single nucleotide polymorphisms (SNPs) of five immunological genes in CD28/B7 pathway were genotyped. Overall, five SNPs filtered by ReliefF algorithm were rs733618, rs11889031, rs4553808, rs4675374 and rs10754339. The best model of multifactor dimensionality reduction (MDR) contained rs733618 and rs11889031. The high risk genotypes combination contributed to increasing risk of breast cancer (odds ratio (OR), 4.36; 95% confidence interval (CI); 3.15-6.02). The information gain (IG) value of these two SNPs was 8.07%, presented strongest interaction effect. Five significant multiplicative interactions and seven significant combining effects were found among the filtered SNPs. Moreover, the filtered SNPs were still stable in the groups of ER(+), PR(+), CerbB2(-) and lymph nodes (LN) involvement positive with the best models including rs733618 and rs11889031. The most frequent haplotype was TACC which significantly increased breast cancer risk (OR, 1.80; 95%CI, 1.43-2.25). These results suggested that interactions among cytotoxic T lymphocyte antigen-4 (CTLA4), inducible co-stimulator (ICOS) and B7H4 might play critical roles on the risk of breast cancer. Copyright © 2015. Published by Elsevier B.V.
    No preview · Article · Apr 2015 · Gene
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    ABSTRACT: OX40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of OX40-OX40L is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. The genetic variants of OX40L can increase the risk of SLE, atherosclerosis, systemic sclerosis and show gender-specific effects in some studies. Accordingly, we performed a case-control study including 557 breast cancer patients and 580 age- and sex-matched healthy controls to investigate whether single nucleotide polymorphisms (SNPs) in the OX40L gene are associated with sporadic breast cancer susceptibility and progression in Chinese Han women. Seven SNPs of OX40L (rs6661173, rs1234313, rs3850641, rs1234315, rs12039904, rs844648 and rs10912580) were genotyped with the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results indicated that rs3850641G allele could increase the susceptibility to breast cancer (P = 0.009662), even in the validation study (P = 0.0001515). A significant association between rs3850641 and breast cancer risk was observed under the additive model and dominant model (P = 0.01042 and 0.01942, respectively). The haplotype analysis showed that haplotype A(rs844648)A(rs10912580) was significantly associated with breast cancer, even after 10,000 permutations for haplotypes in block only (P = 0.0003). In clinicopathologic features analysis, the association between rs1234315 and C-erbB2 status was significant (P = 0.02541). Our data primarily indicates that rs3850641 of OX40L gene contributes to sporadic breast carcinogenesis in a northeast Chinese Han population.
    Full-text · Article · Aug 2012 · PLoS ONE
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    Dataset: Table S6
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    ABSTRACT: Primers, PCR programs, restriction enzyme and restriction fragments for CD40 PCR-RFLP genotyping. (DOC)
    Preview · Dataset · Aug 2011
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    Dataset: Figure S1
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    ABSTRACT: Haplotypic architecture in CD28. Haplotype LD block structure across the CD28 gene was generated from 565 breast cancer patients and 605 healthy individuals in a Chinese Han population using Haploview software. (A) LD prime chart from Haploview that summarizes the LD patterns is shown. The numbers in each box represent the LD value between the adjacent SNPs. (B) r2 prime chart from Haploview that summarizes the r2 patterns is shown. The numbers in each box represent the r2 value between the adjacent SNPs. The physical representations of the SNP positions in (A, B) have been colored to represent the LD between the adjacent SNPs, according to the standard Haploview software color scheme: LOD>2 and D' = 1, red; LOD>2 and D'<1, shades of pink/red; LOD<2 and D' = 1, blue; LOD<2 and D'<1, white. The definition of the LD blocks were based on the method of Gabriel et al. with confidence limits for strong LD (upper, 0.85; lower, 0.70), and confidence interval maximums for strong recombination (upper, 0.85) and strong LD (upper, at least 0.8), in informative comparisons. In order to help keep the display uncluttered, D prime values of 1.0 were never shown (the box is empty). The strongest LD shown above was between rs3116487 and rs3116494 (D’ = 1, r2 = 0.99). Two LD blocks were identified from the CD28 gene are presented. Block 1 covered 14 kb, and block 2 about 5 kb. SNP rs3119686, with minor allele frequency of only 1.75%, was excluded from the haplotype analysis. (TIF)
    Preview · Dataset · Aug 2011
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    Dataset: Table S5
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    ABSTRACT: Relationship between lymph node involvement status in breast cancer patients and variants detected in the CD28 gene. 1LN, lymph node. 2LN involvement information of 543 breast cancer patients was available in the study with 235 (41.59%) positive and 310 (54.87%) negative ones. 3The P values were accessed using Plink and SPSS software under an additive model (AA vs. Aa vs. aa), dominant model (aa+Aa vs. AA), and recessive model (aa vs. aA+AA) respectively. Significant values (P<0.05) are in bold. (DOC)
    Preview · Dataset · Aug 2011
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    ABSTRACT: Breast cancer is a polygenetic disorder with a complex inheritance pattern. Single nucleotide polymorphisms (SNPs), the most common genetic variations, influence not only phenotypic traits, but also interindividual predisposition to disease, treatment outcomes with drugs and disease prognosis. The co-stimulatory molecule CD40 plays a prominent role in immune regulation and homeostasis. Accumulating evidence suggests that CD40 contributes to the pathogenesis of cancer. Here, we set out to test the association between polymorphisms in the CD40 gene and breast carcinogenesis and tumor pathology.
    Preview · Article · Aug 2011 · PLoS ONE
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    Dataset: Table S2
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    ABSTRACT: Significant associations between CD40 SNPs and PR status in patients. (DOC)
    Preview · Dataset · Aug 2011
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    Dataset: Table S1
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    ABSTRACT: Significant associations between CD40 SNPs and ER status in patients. (DOC)
    Preview · Dataset · Aug 2011
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    Dataset: Table S3
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    ABSTRACT: Significant associations between CD40 SNPs and LN involvement status in patients. (DOC)
    Preview · Dataset · Aug 2011
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    Dataset: Table S4
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    ABSTRACT: Significant associations between CD40 SNPs and C-erbB2 status in patients. (DOC)
    Preview · Dataset · Aug 2011
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    ABSTRACT: Infiltrating ductal carcinoma (IDC) is the most common malignant breast cancer in women, and genetic factors appear to play a significant role in the susceptibility to IDC. Alteration of DNA methylation is an epigenetic change in human cancers, including breast cancer. DNA-methyltransferase 1 (DNMT1) is a major enzyme that determines genomic methylation patterns. In order to clarify the association of DNMT1 polymorphisms with IDC, a case-control study was conducted in women from the Heilongjiang Province, in the northeast of China. We scrutinized the 2 genetic polymorphisms in exons of DNMT1 that may influence the activity of DNMT1. Our research subjects consisted of 305 patients with IDC and 314 age-matched healthy controls. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism. Data were analyzed using the χ2 test by SPSS, version 13.0, and Haploview, version 4.1. The association between DNMT1 polymorphisms and the clinical features of IDC was analyzed. In rs16999593, the frequency of CT genotype and C allele were lower in patients than in controls (P = .028 and P = .017, respectively). Also, rs2228611 AG genotype was higher in patients than in controls (P = .015). The frequency of haplotype CA was lower in patients than in controls (P = .034). Significant association was shown between the 2 single nucleotide polymorphisms of the DNMT1 gene and progesterone receptor (PgR) and p53 status. No association was found between DNMT1 gene polymorphisms and tumor size or estrogen receptor status. Our results was a previous study, which suggested that DNMT1 gene polymorphisms in exons may provide valuable information for predicting the sporadic IDC risk and may be associated with prognosis factors such as PgR and p53 status in Chinese Han women in the Heilongjiang Province.
    No preview · Article · Oct 2010 · Clinical Breast Cancer

Publication Stats

19 Citations
10.71 Total Impact Points

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Institutions

  • 2010-2015
    • Harbin Medical University
      • Department of Immunology
      Charbin, Heilongjiang Sheng, China