Qing-Feng Liang

Capital Medical University, Peping, Beijing, China

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Publications (12)5.2 Total impact

  • Qing-Feng Liang · Xu-Guang Sun · Antoine Labbe
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    ABSTRACT: Infectious keratitis remains a severe ocular condition that can lead to irreversible complications and even blindness. An early diagnosis of an infective microorganism leading to effective treatment may improve the outcome of infectious keratitis.However, the accurate and rapid identification of the infectious agent remains a challenge for the ophthalmologist.In vivo confocal microscopy (IVCM) is a noninvasive imaging technique that provides high-resolution images of ocular surface structures at a cellular level and infectious keratitis represents one of its most important clinical uses. The value of IVCM has been demonstrated in the diagnosis of acanthamoeba and filamentous fungal keratitis. The follow-up of such corneal infections may also be improved by this noninvasive imaging technique.However, the current resolution of IVCM limits its use in cases of bacterial and viral keratitis.
    No preview · Article · Oct 2013 · [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
  • Sen Wang · Qing-Feng Liang · Xu-Guang Sun
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    ABSTRACT: Photodynamic therapy(PDT) as a new therapeutic method is increasing widely used in ocular surface disease. Along with intensive studies of microorganism inhibition and promoting function in tissue repairing of PDT have been developed, PDT been gradually applied to treating infectious keratitis in clinical. We review mechanism, sterilization and application in infectious keratitis of PDT.
    No preview · Article · Aug 2013 · [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
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    Zhi-Yuan Chen · Qing-Feng Liang · Guang-Yan Yu
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    ABSTRACT: To establish a rabbit model for keratoconjunctivitis sicca (KCS) to study autologous submandibular gland transfer for treating severe KCS. In 2 groups of 10 rabbits, left eyes were operated and right eyes were controls. In the trichloroacetic acid-treated group, the lacrimal and harderian glands and nictitating membrane were removed surgically; the palpebral and bulbar conjunctiva were swabbed with 50% trichloroacetic acid. In the non-trichloroacetic acid-treated group, the lacrimal and harderian glands and nictitating membrane were surgically removed. The Schirmer test was performed preoperatively and 1, 2, 3, and 4 months postoperatively. Corneal densities of rose bengal and fluorescein staining were scored every month postoperatively. At 4 months, the cornea and bulbar conjunctiva were removed from operated and control eyes for histopathology. The upper bulbar conjunctiva was used to determine goblet cell density. Compared with preoperative conditions, tear secretion of operated eyes significantly decreased in both groups postoperatively, then gradually increased. Scores for corneal rose bengal and fluorescein staining were higher and conjunctival goblet cell density was lower in the operated eyes than in control right eyes in both groups, but no significant difference was found between the operated eyes of the two groups. Inflammatory histopathologic changes of the cornea and conjunctiva were not found in either of the eyes in the two groups. A new rabbit model for KCS could be created by either of these methods. Experimental KCS with reduction of tear production was possible with surgical ablation of the lacrimal and harderian glands and nictitating membrane. It is unnecessary to apply trichloroacetic acid to burn the conjunctiva. Our modified incision better exposed the surgical field.
    Preview · Article · Jun 2011 · Cornea
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    ABSTRACT: Pterygium is a common ophthalmic disease and an important public health problem. It may be affected by many factors such as age, gender, ultraviolet radiation exposure, and time spent outdoors. This study aimed to investigate the prevalence of pterygium and evaluate the risk factors for the development of pterygium in the aged rural population of Beijing. The investigation is a population-based cross-sectional study. Participants from 198 villages of 13 suburban districts, aged 55 - 85 years old, were invited to complete a medical record, and 37 067 individuals were taken external ocular and fundus photos at Beijing ocular disease survey in 2008-2009. The information was uploaded by Internet and diagnosed with stages and grades by ophthalmological specialists. The prevalence, stages and grades of pterygia were observed. Of the 37 067 individuals, 1395 (3.76%) had pterygium. There was a significantly higher prevalence in male (5.13%) than in female (3.17%, P = 0.000). The prevalence rate increased obviously with ages (chi(2) = 7.939, P = 0.019) in rural Beijing. The average prevalence of 5.91% in Daxing and Fangshan districts with low latitude and low precipitation was significantly higher than that in Miyun and Huairou districts with high latitude and deep precipitation (3.17%, P = 0.000). The majority of the pterygia (43.5%) were in active stage and 46.5% pterygia involved pupil area corneas. There is a statistically significant association between latitude and precipitation of habitation region and pterygium formation. Solar radiation, especially ultraviolet exposure represents a significant environment hazard to pterygium development.
    Preview · Article · Jul 2010 · Chinese medical journal
  • Qing-feng Liang · Da-zhai Li · Yi-da Hu · Dong Wei · Shi-jing Deng · Xu-guang Sun
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    ABSTRACT: To design the robotic mechanism of the cornea grafting micro-surgery system and evaluate its experimental feasibility and efficacy. It was an experimental study. Based on computer assisted cornea grafting surgery (CACGS), the prototype about cornea grafting robot, which consists of six subsystems, was developed. The system was used for looping incision and suturing for 20 rabbit corneas. Experimental data were studied including the error of target incision position, the actual cutting depth and the cutting edge span in order to evaluate the system's clinical value. All above statistical descriptions were performed using SPSS software version 11.5. 20 corneas were looping incised and sutured by the robot-assisted cornea grafting micro-surgery system successfully. The error of target incision position was (0.356 + or - 0.040) mm. With CACGS, the cutting edge span (A) was (0.855 + or - 0.040) and the actual cutting depth error was less than 10 microm. Corneal structure and thickness was showed no change and cutting edge of cornea was displayed regularity in pathological examination. To suturing system, suturing span was (2.15 + or - 0.09) mm and the error of span (0.15 + or - 0.02) mm. The cornea grafting micro-surgery system developed is used for cornea grafting of some animals successfully. The experiment results demonstrate the stability and efficiency of the robot.
    No preview · Article · Sep 2009 · [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
  • Qing-feng Liang · Xiu-ying Jin · Xiang-lan Wang · Xu-guang Sun
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    ABSTRACT: Fungal keratitis is a rare but serious corneal disease that may result in loss of vision. The poor prognosis might be due to limited treatment option. This study aimed to evaluate the clinical efficacy of 0.25% terbinafine eye drops comparing with 5% natamycin suspension on fungal keratitis. A retrospective clinical trial was performed on 90 patients presenting with direct smear and/or culture positive fungal keratitis at Beijing Tongren Hospital, Beijing, China from January 2006 to May 2008. Corneal ulcers were categorized as mild or severe. Forty-five patients were treated with topical terbinafine and the next 45 cases received topical natamycin hourly. Filamentous fungi were found in corneal scrapings among all 90 cases. Fungal cultures were positive in 64 patients (71%). Species of Fusarium and Aspergillus were the principal isolates. Forty (89%) patients showed favorable response to terbinafine, while forty-two (93%) patients exhibited favorable response to natamycin (P > 0.05). The mean course of treatment was significantly showed in the terbinafine treatment group than natamycin group ((26.5 +/- 11.2) days versus (19.3 +/- 6.4) days; P < 0.05). In terbinafine group, twenty patients with ulcers smaller than 4 mm had favorable outcome, while 20 of 25 patients with ulcers more than 4 mm in diameter had favorable response (P < 0.05). Twenty-seven patients with depth of infiltration less than half of stroma thickness had favorable response to terbinafine, while 13 of 18 patients with depth of infiltration more than half of stroma responded to terbinafine. This difference was statistically significant (P < 0.05). Our findings suggest that topical terbinafine is an effective antifungal drug for the management of filamentous mycotic keratitis, particularly in cases with smaller and shallower ulcers. Its mean duration of treatment was longer than natamycin.
    No preview · Article · Aug 2009 · Chinese medical journal
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    ABSTRACT: To analyze the types, distribution characteristics and in vitro susceptibility of antibiotic agents of ocular non-viral microbial isolates in children. Experimental research.785 specimens obtained from children below the age of 14 in Beijing Tongren Hospital during 1999 to 2004 were cultured and identified for bacterium, fungus and Acanthamoeba respectively at Beijing Institute of Ophthalmology. Microbial culture-positive rate, susceptibility patterns and the organisms distribution of positive cultures were retrospectively analyzed. The results were statistical analyzed by chi(2). Of the 785 specimens, 211 were culture positive. The percentage of positive culture was 26.88%. Of those positive cultures, 176 (83.41%) had pure bacterial infections, 32 (15.17%) had pure fungal infections, and 3 (1.42%) had pure cultures of Acanthamoeba. Of the 176 bacterial positive cultures, polymicrobial infection was present in 38 cases (33 bacterial, 5 fungal and bacterial). Of 214 bacterial isolates, the most common isolate was Neisseria gonorrhoeae (39/214, 18.22%), followed by Staphylococcus epidermidis (37/214, 17.29%), and Corynebacterium spp. (36/214, 16.82%). The most common fungal pathogen isolated was Fusarium spp. representing 25.00% (8/32) of all positive fungal cultures, followed by Aspergillus spp. (15.63%, 5/32). The sensitivity of tobramycin, norfloxacin, gentamicin, amikacin, ciprofloxacin, ofloxacin, rifampicin and levofloxacin of bacteria was 62.57%, 64.94%, 70.06%, 70.87%, 71.19%, 73.89%, 85.80% and 87.50%. Gram-positive isolates were susceptible to levofloxacin. Gram-negative isolates showed high susceptible to ciprofloxacin, ofloxacin and levofloxacin. The fungal isolates were most susceptible to natamycin, followed by terbinafine, but resistant to fluconazole. Bacteria are the most common pathogens in non-viral microbial eye infection in children. Neisseria gonorrhoeae, Staphylococcus epidermidis, and Corynebacterium spp. are the most common bacterial pathogens, which are highly susceptible to levofloxacin and rifampicin. Fusarium spp. and Aspergillus spp. are the most common fungal pathogens, which are highly susceptible to natamycin but resistant to fluconazole.
    No preview · Article · Mar 2009 · [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
  • Qing-Feng Liang · Zhi-Qun Wang · Ran Li · Shi-Yun Luo · Shi-Jing Deng · Xu-Guang Sun
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    ABSTRACT: To establish a method to measure mutant prevention concentration (MPC) in vitro, and to measure MPC of antibacterial agents for ocular bacteria caused keratitis. It was an experimental study. Forty strains of ocular bacteria were separated from cornea in Beijing Institute of Ophthalmology, which included 8 strains of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Pseudomonas aeruginosa and Klebsiella pneumoniae respectively. The minimal inhibitory concentration (MIC) of the levofloxacin (LVF), ofloxacin (OFL), ciprofloxacin (CIP), norfloxacin (NFL), tobramycin (TOB) and chloromycetin (CHL) were determined by agar dilution method from National Committee of Clinical Laboratory Standard (NCCLS). The MPC were measured by accumulate-bacterial methods with bacterial population inoculated more than 1.2 x 10(10) colony forming units per milliliter with Mueller-Hinton broth and tryptic soy agar plate. With the software of SPSS 11.0, the datum such as the range of MIC, MPC, MIC90 and MPC90 were calculated, and the selection index (MPC90/ MI90) and mutant selection window (MSW) were obtained. The MI90 of LVF and TOB (4 mg/L) to Staphylococcus aureus strains were the lowest. CIP showed the lowest MIC90 (0.25 mg/L) to Pseudomonas aeruginosa among six kinds of antibacterial agents. The MIC90 of LVF to Staphylococcus epidermidis (256 mg/L), Streptococcus pneumoniae (1 mg/L) and Klebsiella pneumoniae (0.25 mg/L) were lower than other antibacterial agents. The MPC90, MSW and the MPC90/MIC90 of levofloxacin showed lower values compared with other antibacterial medicines. From all the datum, the MIC90 of CHL was the highest and the activity was the weakest. Although the activity of LVF was higher to every kind of bacteria, CIP had the highest activity antibacterial to Pseudomonas aeruginosa. The capacity of CHL and TOB was weaker than Quinolones for restricting resistant mutants on ocular bacteria. LVF had the strongest capacity for restricting resistant mutants among Quinolones. LVF has better antibacterial effects and stronger capacity for restricting the selection of resistant mutants on ocular bacteria than other antibacterial agents.
    No preview · Article · Feb 2009 · [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
  • Zhe Ren · Qing-feng Liang · Sheng Guo · Yu-mei Zhou · Ran Li · Xu-guang Sun
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    ABSTRACT: To assess the prevalence and risk factors for trachoma among primary school children in Datong City, China. An epidemiological survey was carried out in Datong City in 2006. Children aged 6 to 14 years old in primary school were selected by a cluster sampling in which class shift was the sampling unit. Trachoma was clinically assessed according to the simplified classification for trachoma proposed by the World Health Organization (WHO). If the child was clinically diagnosed as trachoma, a conjunctival swab would be taken for detecting C. trachomatis by antigen test and polymerase chain reaction (PCR). A total of 1236 children were examined and 135 cases of trachoma were found, the prevalence of trachoma was 10.9% (95% CI: 9.2% - 12.6%). Follicular trachoma was found in 86.7% of the cases and intense trachoma in 13.3%. The overall prevalence of trachoma was higher for females than for males (13.1% versus 8.8%, P = 0.016). Thirteen (9.6%) cases were positive in C. trachomatis antigen test, and 86 (63.7%) cases were positive by PCR. Poor ocular hygiene and sharing wash cloth and face basin with households were the main risk factors. Trachoma is endemic among primary school children in Datong City in China.
    No preview · Article · Jul 2008 · [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
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    ABSTRACT: To assess the in vitro susceptibilities of ocular bacterial isolates to Gatifloxacin and other fluoroquinolones which are often used in ophthalmic treatment. Non-randomized, non-comparative, retrospective study. Ocular bacterial isolates (n = 619) from Beijing Institute of Ophthalmology were tested for their susceptibilities to gatifloxacin, ciprofloxacin, ofloxacin and levofloxacin in vitro by the Kirby-Bauer disk diffusion method. The total number of ocular bacterial isolates was 619 from January, 2005 to December, 2006 including gram-positive cocci 372(60.1%), gram-negative cocci 7 (1.1%), gram-positive bacilli 60 (9.7%), gram-negative bacilli 177 (28.6%), other 3 (0.5%). The percentage of susceptibility in total ocular bacterial isolates to gatifloxacin, ciprofloxacin, ofloxacin and levofloxacin was 88.4%, 72.1%, 64.8% and 54.4%. The susceptibility of Staphylococcus species to gatifloxacin (89.9%) was significantly higher than ciprofloxacin (38.8%), ofloxacin (48.2%) and levofloxacin (61.6%). Streptococcus species showed significantly higher susceptibility to gatifloxacin (93.2%), levofloxacin (89.2%) and ofloxacin (87.8%) than ciprofloxacin (62.2%). The susceptibility of gram-positive bacilli to gatifloxacin (86.7%) was significantly higher than ciprofloxacin (58.3%); All above differences are significant (P < 0.0083). Gram-negative cocci, enteric bacilli family and Pseudomonas species have the equivalent susceptibility to gatifloxacin, ciprofloxacin, ofloxacin and levofloxacin (P > 0.05). Most of ocular bacterial are susceptible to gatifloxacin in vitro, especially gram-positive bacteria including Staphylococcus species, Streptococcus species and gram-positive bacilli.
    No preview · Article · Apr 2008 · [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
  • Qing-feng Liang · Xu-guang Sun · Ying Li · Zhi-qun Wang · Shi-yun Luo · Ran Li
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    ABSTRACT: Laser in situ keratomileusis (LASIK) is currently the most popular keratorefractive procedure performed worldwide with generally excellent outcomes. I However, infectious keratitis after LASIK is a devastating, vision-threatening complication. The common pathogens 2 were bacteria and fungi. Nontuberculous mycobacterium (NTM) accounted for a substantial proportion of these pathogens. Most NTM infections (86% of cases) were 2 involved in unilateral cornea. In this study, we report six patients with bilateral NTM keratitis following LASIK.
    No preview · Article · Dec 2007 · Chinese medical journal
  • Qing-feng Liang · Zhi-qun Wang · Shi-jing Deng · Ran Li · Shi-yun Luo · Xu-guang Sun
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    ABSTRACT: To study clinical manifestation and pathology change of different time points of rabbit keratitis caused by non-tuberculous mycobacteria (NTM). Forty-eight eyes from 48 rabbits were randomly divided into three groups:under-flap infection group (UFI group), under-flap infection group with corticosteroid (UFIC group) and surface infection group (SI group). The cornea infiltration area of all rabbits was observed. Bacterial quantitative culture and histopathological examination were performed. In the earliest stage (5 days), the cornea was cloudy and edematous. In the middle stage (7 - 14 days), cornea stromal infiltration became its major clinical sign. In the last stage (21 days), corneal neovascularization and fibrosis were developed. The mean stromal infiltration area in UFIC group was greater than those of UFI group and SI group (F = 9.362, 8.341, P < 0.05). The number of NTM in UFIC group was higher than those of UFI group and SI group (F = 411.272, P < 0.05). In histopathological examination, there were infiltrates with polymorphonuclear leukocytes (PMN) in the corneal stroma in the early stage and acid-fast bacilli was found. A plenty of lymphocytes in the cornea was found in the middle stage of NTM keratitis. In the latter stage, the fibroblast cells proliferation and corneal ulcer were cured. During the course of this infection, the number of CD4+, CD8+ T lymphocyte increased significantly, and then decreased gradually following the regress of the infection. In the stage (5 - 21 days), CD4+ T lymphocyte decreased significantly in UFIC group compared with other two groups (F = 21.907, 196.521, 12.552, 11.100, P < 0.01). The clinical characteristic of rabbit keratitis is the multifocal dense superficial stromal infiltrates. CD4+ T lymphocyte may play an important role in NTM keratitis.
    No preview · Article · Aug 2007 · [Zhonghua yan ke za zhi] Chinese journal of ophthalmology

Publication Stats

47 Citations
5.20 Total Impact Points


  • 2008-2013
    • Capital Medical University
      Peping, Beijing, China