Conference Paper: Use of a photonic lantern into an image plane fibre beam combiner
- [Show abstract] [Hide abstract] ABSTRACT: A signal based algorithm resulting in increased depth resolution is presented for medical ultrasound. It relies on multiple foci beamforming that is enabled by current ultrasound imaging systems. The concept stems from optical microscopy and is translated here into ultrasound using the Field II simulation software. A 7 MHz linear transducer is used to scan a single point scatterer phantom that can move in the axial direction. Individual beamformer outputs from 3 different foci are post-processed using the highly-dependent on focusing errors, metric of sharpness to estimate the position of the point scatter. A 37.8 μm uncertainty in depth estimation is achieved, which attains an almost 3-fold improvement compared to conventional ultrasound imaging axial resolution. Future work on the development of this algorithm requires experimental validation in tissue-like materials that provide strong aberrations.
- [Show abstract] [Hide abstract] ABSTRACT: Recent advances in adaptive optics (AO) have led to the implementation of wide field-of-view AO systems. A number of wide-field AO systems are also planned for the forthcoming Extremely Large Telescopes. Such systems have multiple wavefront sensors of different types, and usually multiple deformable mirrors (DMs). Here, we report on our experience integrating cameras and DMs with the real-time control systems of two wide-field AO systems. These are CANARY, which has been operating on-sky since 2010, and DRAGON, which is a laboratory adaptive optics real-time demonstrator instrument. We detail the issues and difficulties that arose, along with the solutions we developed. We also provide recommendations for consideration when developing future wide-field AO systems.
- [Show abstract] [Hide abstract] ABSTRACT: An ultrasound imaging technique providing sub-diffraction limit axial resolution for point sources is proposed. It is based on simultaneously acquired multi-focal images of the same object, and on the image metric of sharpness. The sharpness is extracted by image data and presents higher values for in-focus images. The technique is derived from biological microscopy and is validated here with simulated ultrasound data. A linear array probe is used to scan a point scatterer phantom that moves in depth with a controlled step. From the beamformed responses of each scatterer position the image sharpness is assessed. Values from all positions plotted together form a curve that peaks at the receive focus, which is set during the beamforming. Selection of three different receive foci for each acquired dataset will result in the generation of three overlapping sharpness curves. A set of three calibration curves combined with the use of a maximum-likelihood algorithm is then able to estimate, with high precision, the depth location of any emitter fron each single image. Estimated values are compared with the ground truth demonstrating that an accuracy of 28.6 μm (0.13λ) is achieved for a 4 mm depth range.
- [Show abstract] [Hide abstract] ABSTRACT: This paper compares experimentally temporal and subband implementations of the Minimum Variance (MV) adaptive beamformer for medical ultrasound imaging. The performance of the two approaches is tested by comparing wire phantom measurements, obtained by the research ultrasound scanner SARUS. A 7 MHz BK8804 linear transducer was used to scan a wire phantom in which wires are separated by 10 mm. Performance is then evaluated by the lateral Full-Width-Half-Maximum (FWHM), the Peak Sidelobe Level (PSL), and the computational load. Beamformed single emission responses are also compared with those from conventional Delay-and-Sum (DAS) beamformer. FWHM measured at the depth of 46.6 mm, is 0.02 mm (0.09λ) for both adaptive methods while the corresponding values for Hanning and Boxcar weights are 0.64 and 0.44 mm respectively. Between the MV beamformers a -2 dB difference in PSL is noticed in favor of the subband approach (-31 and -33 dB), whereas values from conventional are not lower than -29 dB. This slight improvement in the case of the subband implementation comes at the expense of increased computational cost; 3.7 TFLOPs per image are required in contrast to 130 GFLOPs of the temporal one, when only 0.5 GFLOPs are needed in DAS beamforming.
- [Show abstract] [Hide abstract] ABSTRACT: This paper compares the performance between temporal and subband Minimum Variance (MV) beamformers for medical ultrasound imaging. Both adaptive methods provide an optimized set of apodization weights but are implemented in the time and frequency domains respectively. Their performance is evaluated with simulated synthetic aperture data obtained from Field II and is quantified by the Full-Width-Half-Maximum (FWHM), the Peak-Side-Lobe level (PSL) and the contrast level. From a point phantom, a full sequence of 128 emissions with one transducer element transmitting and all 128 elements receiving each time, provides a FWHM of 0.03 mm (0.14λ) for both implementations at a depth of 40 mm. This value is more than 20 times lower than the one achieved by conventional beamforming. The corresponding values of PSL are -58 dB and -63 dB for time and frequency domain MV beamformers, while a value no lower than -50 dB can be obtained from either Boxcar or Hanning weights. Interestingly, a single emission with central element #64 as the transmitting aperture provides results comparable to the full sequence. The values of FWHM are 0.04 mm and 0.03 mm and those of PSL are -42 dB and -46 dB for temporal and subband approaches. From a cyst phantom and for 128 emissions, the contrast level is calculated at -54 dB and -63 dB respectively at the same depth, with the initial shape of the cyst being preserved in contrast to conventional beamforming. The difference between the two adaptive beamformers is less significant in the case of a single emission, with the contrast level being estimated at -42 dB for the time domain and -43 dB for the frequency domain implementation. For the estimation of a single MV weight of a low resolution image formed by a single emission, 0.44 * 109 calculations per second are required for the temporal approach. The same numbers for the subband approach are 0.62 * 109 for the point and 1.33 * 109 for the cyst phantom. The comparison demonstrates similar resolution but slightly lower side-lobes and higher contrast for the subband approach at the expense of increased computation time.
- [Show abstract] [Hide abstract] ABSTRACT: A phase diversity wavefront sensor includes an optical system including at least one optical element for receiving a light beam; a diffractive optical element having a diffractive pattern defining a filter function, the diffractive optical element being arranged to produce, in conjunction with the optical system, images from the light beam associated with at least two diffraction orders; and a detector for detecting the images and outputting image data corresponding to the detected images. In one embodiment, the optical system, diffractive optical element, and detector are arranged to provide telecentric, pupil plane images of the light beam. A processor receives the image data from the detector, and executes a Gerchberg-Saxton phase retrieval algorithm to measure the wavefront of the light beam.
- [Show abstract] [Hide abstract] ABSTRACT: We have built a HOE-based display capable of reconstructing arbitrary images, in mid-air at fixed focal depths, that can interact with the viewer in real-time. The display system comprises the HOE, a laser projection subsystem, a Kinect motion sensor and an embedded controller. The HOE functions as a fast converging lens and is A4 page sized (20×30cm). We have written a number of simple apps for the display that allow the user to draw in mid-air or to touch icons and buttons that trigger other actions. The reconstructed holographic images are high-resolution, relatively bright and visible under ambient indoor lighting conditions.
- [Show abstract] [Hide abstract] ABSTRACT: It is shown that grisms, a grating and prism combination, are a simple way to achieve chromatic control in 3D multi-plane imaging. A pair of grisms, whose separation can be varied, provide a collimated beam with a tuneable chromatic shear from a collimated polychromatic input. This simple control permits the correction of chromatic smearing in 3D imaging using off-axis Fresnel zone plates and improved control of the axial profile of a focussed spot in multi-photon experiments. (C) 2012 Optical Society of America
- [Show abstract] [Hide abstract] ABSTRACT: We extend the redundant spacings calibration method for finding piston coefficients affecting the elements of a dilute aperture array so that tilt phase coefficients can also be calculated and corrected without the need for assumptions about the object. The tilt coefficient retrieval method is successfully demonstrated in simulation, and the specifics of correction by image sharpness are discussed, showing that in dilute aperture systems this method does not necessarily produce a unique image. (C) 2012 Optical Society of America
- [Show abstract] [Hide abstract] ABSTRACT: Image denoising and signal enhancement are two common steps to improve particle contrast for detection in low-signal-to-noise ratio (SNR) fluorescence live-cell images. However, denoising may oversmooth features of interest, particularly weak features, leading to false negative detection. Here, we propose a robust framework for particle detection in which image denoising in the grayscale image is not needed, so avoiding image oversmoothing. A key to our approach is the new development of a particle enhancement filter based on the recently proposed particle probability image to obtain significantly enhanced particle features and greatly suppressed background in low-SNR and low-contrast environments. The new detection method is formed by combining foreground and background markers with watershed transform operating in both particle probability and grayscale spaces; dynamical switchings between the two spaces can optimally make use the information in images for accurate determination of particle position, size, and intensity. We further develop the interacting multiple mode filter for particle motion modeling and data association by incorporating the extra information obtained from our particle detector to enhance the efficiency of multiple particle tracking. We find that our methods lead to significant improvements in particle detection and tracking efficiency in fluorescence live-cell applications.
- [Show abstract] [Hide abstract] ABSTRACT: This paper discusses a range of phase-diversity and tracking applications that have been demonstrated experimentally, and will present an analysis of experimental errors associated with the system used. Simultaneous imaging in multiple imaging modes is demonstrated and the use of wavefront-sensing techniques to achieve nanometric depth resolution is reviewed.
- [Show abstract] [Hide abstract] ABSTRACT: We describe a method for tracking the position of small features in three dimensions from images recorded on a standard microscope with an inexpensive attachment between the microscope and the camera. The depth-measurement accuracy of this method is tested experimentally on a wide-field, inverted microscope and is shown to give approximately 8 nm depth resolution, over a specimen depth of approximately 6 µm, when using a 12-bit charge-coupled device (CCD) camera and very bright but unresolved particles. To assess low-flux limitations a theoretical model is used to derive an analytical expression for the minimum variance bound. The approximations used in the analytical treatment are tested using numerical simulations. It is concluded that approximately 14 nm depth resolution is achievable with flux levels available when tracking fluorescent sources in three dimensions in live-cell biology and that the method is suitable for three-dimensional photo-activated localization microscopy resolution. Sub-nanometre resolution could be achieved with photon-counting techniques at high flux levels.
- [Show abstract] [Hide abstract] ABSTRACT: Fluorescence imaging of dynamical processes in live cells often results in a low signal-to-noise ratio. We present a novel feature-preserving non-local means approach to denoise such images to improve feature recovery and particle detection. The commonly used non-local means filter is not optimal for noisy biological images containing small features of interest because image noise prevents accurate determination of the correct coefficients for averaging, leading to over-smoothing and other artifacts. Our adaptive method addresses this problem by constructing a particle feature probability image, which is based on Haar-like feature extraction. The particle probability image is then used to improve the estimation of the correct coefficients for averaging. We show that this filter achieves higher peak signal-to-noise ratio in denoised images and has a greater capability in identifying weak particles when applied to synthetic data. We have applied this approach to live-cell images resulting in enhanced detection of end-binding-protein 1 foci on dynamically extending microtubules in photo-sensitive Drosophila tissues. We show that our feature-preserving non-local means filter can reduce the threshold of imaging conditions required to obtain meaningful data.
- [Show abstract] [Hide abstract] ABSTRACT: A simple low resolution volumetric display is presented, based on holographic volume-segments. The display system comprises a proprietary holographic screen, laser projector, associated optics plus a control unit. The holographic screen resembles a sheet of frosted glass about A4 in size (20x30cm). The holographic screen is rear-illuminated by the laser projector, which is in turn driven by the controller, to produce simple 3D images that appear outside the plane of the screen. A series of spatially multiplexed and interleaved interference patterns are pre-encoded across the surface of the holographic screen. Each illumination pattern is capable of reconstructing a single holographic volume-segment. Up to nine holograms are multiplexed on the holographic screen in a variety of configurations including a series of numeric and segmented digits. The demonstrator has good results under laboratory conditions with moving colour 3D images in front of or behind the holographic screen.
- [Show abstract] [Hide abstract] ABSTRACT: A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving approximately 10 nm z position resolution by parameterising the images with an image sharpness metric.
- [Show abstract] [Hide abstract] ABSTRACT: A feature-preserving non-local means (FP-NLM) filter has been developed recently for denoising images containing small and weak particlelike objects. It explores the commonly used non-local means filter to employ two similarity measurements taken in the original greyscale image and a feature image which measures the particle probability in the original image. In this paper, we report a new approach to image mapping for constructing the feature image by incorporating both spatial and temporal (2D+t) characteristics of objects. We present a 2D+t FP-NLM filter based on the improved particle probability image. Experiments show that the new filter can achieve better balance between particle enhancement and background smoothing for images under severe noise contamination and has a greater capability in detecting particles of interest in such environments. © 2010. The
- [Show abstract] [Hide abstract] ABSTRACT: We present the application of wavefront sensing to three-component, three-dimensional micro particle tracking velocimetry (μPTV). The technique is based upon examining the defocus of the wavefront scattered by a tracer particle and from such information establishing the 3-D tracer location. The imaging system incorporates a cylindrical lens acting as an anamorphic element that creates different magnifications in the two orthogonal axes. A single anamorphic image is obtained from each tracer, which contains sufficient information to reconstruct the wavefront defocus and uniquely identify the tracer’s axial position. A mathematical model of the optical system is developed and shows that the lateral and depth performance of the sensor can be largely independently varied across a wide range. Hence, 3-D image resolution can be achieved from a single viewpoint, using simple and inexpensive optics and applied to a wide variety of microfluidic or biological systems. Our initial results show that an uncertainty in depth of 0.18 μm was achieved over a 20-μm range. The technique was employed to measure the 3-D velocity field of micron-sized fluorescent tracers in a flow within a micro channel, and an uncertainty of 2.8 μm was obtained in the axial direction over a range of 500 μm. The experimental results were in agreement with the expected fluid flow when compared to the corresponding CFD model. Thus, wavefront sensing proved to be an effective approach to obtain quantitative measurements of three-component three-dimensional flows in microfluidic devices.
- [Show abstract] [Hide abstract] ABSTRACT: The principle of redundant spacings calibration has previously been described for the purpose of calibrating piston phase aberration affecting elements of a dilute aperture array using a system of linear equations in terms of the aperture phases as well as object phase information. Here we develop matrices for the correction of piston phase aberration by use of image sharpness and also by phase retrieval. These are both presented in wavefront sensor formulation in order to draw analogy between the approaches. We then discuss solution ambiguity affecting both methods and describe array design criteria to prevent such ambiguity. The problem of increased image aliasing under image sharpness correction is also highlighted.
Article: Analysis of Pupil Replication[Show abstract] [Hide abstract] ABSTRACT: Pupil replication and hypertelescope systems for imaging telluric exoplanets in scattered light are treated. Analytic expressions for the spread functions in one and two dimensions and in the presence of various forms of error are given. Error effects considered include aperture misalignment, tilts, piston, pointing errors, and unequal beam amplitude. The performance of the two approaches is contrasted, and the analytic results are compared with simulation results.
Edinburgh, SCT, United Kingdom
- School of Engineering and Physical Sciences
Sapporo-shi, Hokkaido, Japan
- Division of Media and Network Technologies
University of GroningenGroningen, Groningen, Netherlands