Xiaohua Huang

Nanjing Normal University, Nan-ching, Jiangsu, China

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Publications (107)340.67 Total impact

  • Huiqing Hu · Lihong Wang · Yueli Li · Jingwen Sun · Qing Zhou · Xiaohua Huang
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    ABSTRACT: A great deal of literature is available regarding the environmental and ecological effects of rare earth element pollution on plants. These studies have shown that excess lanthanum (La) (III) in the environment can inhibit plant growth and even cause plant death. Moreover, inhibition of plant photosynthesis is known to be one of the physiological bases of these damages. However, the mechanism responsible for these effects is still unclear. In this study, the mechanism of La(III)-induced damage to plant photosynthesis was clarified from the viewpoint of the chloroplast ultrastructure, the contents of chloroplast mineral elements and chlorophyll, the transcription of chloroplast ATPase subunits and chloroplast Mg2+–ATPase activity, in which rice was selected as a study object. Following treatment with low level of La(III), the chloroplast ultrastructure of rice was not changed, and the contents of chloroplast mineral elements (Mg, P, K, Ca, Mn, Fe, Ni, Cu, and Zn) increased, but the chlorophyll content did not change significantly. Moreover, the transcription of chloroplast ATPase subunits, chloroplast Mg2+–ATPase activity, the net photosynthetic rate and growth indices increased. Following treatment with high levels of La(III), the chloroplast ultrastructure was damaged, chloroplast mineral elements (except Cu and Zn) and chlorophyll contents decreased, and the transcription of chloroplast ATPase subunits, chloroplast Mg2+–ATPase activity, the net photosynthetic rate and growth indices decreased. Based on these results, a possible mechanism of La(III)-induced damage to plant photosynthesis was proposed to provide a reference for scientific evaluation of the potential ecological risk of rare earth elements in the environment.
    No preview · Article · May 2016 · Ecotoxicology and Environmental Safety
  • Xuanbo Zhang · Lihong Wang · Anhua Zhou · Qing Zhou · Xiaohua Huang
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    ABSTRACT: The extensive use of rare earth elements (REEs) has increased their environmental levels. REE pollution concomitant with acid rain in many agricultural regions can affect crop growth. Cytosol free calcium ions (Ca(2+)) play an important role in almost all cellular activities. However, no data have been reported regarding the role of cytosol free Ca(2+) in plant roots simultaneously exposed to REE and acid rain. In this study, the effects of exposures to lanthanum(III) and acid rain, independently and in combination, on cytosol free Ca(2+) levels, root activity, metal contents, biomass, cytosol pH and La contents in horseradish roots were investigated. The simultaneous exposures to La(III) and acid rain increased or decreased the cytosol free Ca(2+) levels, depending on the concentration of La(III), and these effects were more evident than independent exposure to La(III) or acid rain. In combined exposures, cytosol free Ca(2+) played an important role in the regulation of root activity, metal contents and biomass. These roles were closely related to La(III) dose, acid rain strength and treatment mode (independent exposure or simultaneous exposure). A low concentration of La(III) (20mgL(-1)) could alleviate the adverse effects on the roots caused by acid rain, and the combined exposures at higher concentrations of La(III) and acid rain had synergic effects on the roots.
    No preview · Article · Apr 2016 · Ecotoxicology and Environmental Safety
  • Huiqing Hu · Lihong Wang · Qing Zhou · Xiaohua Huang
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    ABSTRACT: Acid rain and rare earth element (REE) pollution exist simultaneously in many agricultural regions. However, how REE pollution and acid rain affect plant growth in combination remains largely unknown. In this study, the combined effects of simulated acid rain and lanthanum chloride (LaCl3) on chloroplast morphology, chloroplast ultrastructure, functional element contents, chlorophyll content, and the net photosynthetic rate (P n) in rice (Oryza sativa) were investigated by simulating acid rain and rare earth pollution. Under the combined treatment of simulated acid rain at pH 4.5 and 0.08 mM LaCl3, the chloroplast membrane was smooth, proteins on this membrane were uniform, chloroplast structure was integrated, and the thylakoids were orderly arranged, and simulated acid rain and LaCl3 exhibited a mild antagonistic effect; the Mg, Ca, Mn contents, the chlorophyll content, and the P n increased under this combined treatment, with a synergistic effect of simulated acid rain and LaCl3. Under other combined treatments of simulated acid rain and LaCl3, the chloroplast membrane surface was uneven, a clear “hole” was observed on the surface of chloroplasts, and the thylakoids were dissolved and loose; and the P n and contents of functional elements (P, Mg, K, Ca, Mn, Fe, Ni, Cu, Zn and Mo) and chlorophyll decreased. Under these combined treatments, simulated acid rain and LaCl3 exhibited a synergistic effect. Based on the above results, a model of the combined effects of simulated acid rain and LaCl3 on plant photosynthesis was established in order to reveal the combined effects on plant photosynthesis, especially on the photosynthetic organelle-chloroplast. Our results would provide some references for further understanding the mechanism of the combined effects of simulated acid rain and LaCl3 on plant photosynthesis.
    No preview · Article · Jan 2016 · Environmental Science and Pollution Research
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    ABSTRACT: A new self-tracking nanoscale drug delivery system has been developed to monitor the drug delivery and release in the tumor cells. The small molecule nanodrug was constructed by conjugation and self-assembly of two widely used anticancer drugs, hydrophilic irinotecan (Ir) with blue fluorescence and hydrophobic doxorubicin (DOX) with red fluorescence, which produced colorful fluorescence variations during the drug delivery and release in cells. Owing to the fluorescence resonance energy transfer (FRET), the Ir-DOX conjugate emitted strong red fluorescence when excited at short wavelength. Benefiting from its amphiphilicity, the Ir-DOX conjugate self-assembled into micelles in aqueous medium and the fluorescence was quenched due to the aggregation-caused quenching (ACQ). No obvious red or blue fluorescence was observed in 12 h cell incubation with Ir-DOX, indicating the Ir-DOX entered cells in the form of micelles rather than free conjugate or free drugs. With increasing incubation time, the broken of Ir-DOX linkage resulted in the release of both free drugs, leading to the recovery of dual-color fluorescence. In vitro cytotoxicity studies showed that the Ir-DOX micelles could overcome the multidrug resistance (MDR) of tumor cells, resulting in a prominent growth inhibition against cancer cell proliferation. The Ir-DOX small molecule nanodrug provides a new design for real-time self-tracking the carrier-free and probe-free drug delivery systems in cancer treatment.
    No preview · Article · Jan 2016 · RSC Advances
  • Chanjuan Liu · Xianglin Pei · Mei Mei · Guoquan Chou · Xiaohua Huang · Chun Wei
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    ABSTRACT: A novel aromatic fluorinated diamine monomer, 3,3′-diisopropyl-4,4′-diaminodiphenyl-4′′-trifluoromethyltoluene, was synthesized by coupling of 2-isopropylaniline and 4-(trifluoromethyl)benzaldehyde and its structure was confirmed by Fourier transform infrared spectroscopy, nuclear magnetic resonance, elemental analysis, and mass spectrometry, and then used to prepare a series of fluorinated polyimides (FPIs) by polycondensation reaction with various commercial dianhydrides via conventional one-step method. The obtained FPIs present excellent solubility in most organic solvents and enough to cast into tough and flexible films. All the FPI films show good optical transparency and light color with the cutoff wavelengths in range of 307–362 nm and the average transmittance above 86%. These polymer films also exhibit high glass transition temperature in range of 261–331°C and thermal stability with 10% weigh loss above 463°C under nitrogen atmosphere. Furthermore, they exhibit outstanding mechanical properties with tensile strengths of 65.9–94.3 MPa, elongation at break of 11.4–13.8%, Young’s modulus of 1.6–1.9 GPa, and low dielectric constants in range of 2.75–3.10 at 1 MHz as well as prominent hydrophobicity with the contact angle in the range of 87.3–93.9°.
    No preview · Article · Dec 2015 · High Performance Polymers
  • Xiaohua Huang · Xianglin Pei · Lichun Wang · Mei Mei · Chanjuan Liu · Chun Wei
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    ABSTRACT: A new diamine monomer, 3,3′-diisopropyl-4,4′-diaminophenyl-4′-phenyltouene, was designed, synthesized, and then polymerized with five commercial dianhydrides to obtain a series of novel polyimides via a one-step method. The obtained polymers showed excellent solubility in most common solvents, even in low-boiling solvents, such as chloroform, dichloromethane, and tetrahydrofuran. They exhibited a high thermal stability with the glass-transition temperature in the range 262-318°C and 10% weight loss temperatures in the range 464-488°C under a nitrogen atmospheres. Meanwhile, these polymer films also displayed a high optical transparency with a cutoff wavelength in the range 305-365 nm; prominent mechanical properties with a tensile strength of 65.6-94.9 MPa, a Young's modulus of 1.6-2.8 GPa, and an elongation at break of 9.3-13.7%; a low dielectric constant in the range of 2.91-3.18 at 1 MHz; and an outstanding hydrophobicity with a contact angle above 90.6°.
    No preview · Article · Dec 2015 · Journal of Applied Polymer Science
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    ABSTRACT: Mevalonate diphosphate decarboxylase (GbMVD) of Ginkgo biloba catalyzes the final step of mevalonic acid pathway by converting the six-carbon mevalonate diphosphate into isopentenyl diphosphate, a precursor of ginkgolides and bilobalide collectively termed terpene trilactones (TTLs). This paper presents the isolation of a 1.2-kb fragment (designated as GbMVDpro) of the 5′-flanking region of GbMVD. Extensive sequence analysis revealed the presence of evolutionarily conserved putative cis-acting elements in light-regulated transcription, hormone signaling (abscisic acid, jasmonate, and salicylic acid), and plant defense (W-box/WRKY) in the GbMVD promoter region. Electrophoretic mobility shift analysis suggested possible involvement of W-box in GbMVD promoter function. To assess the organ-specific and environmentally responsive characteristics of GbMVD expression, GbMVDpro was fused to GUS reporter gene. GbMVDpro::GUS was introduced into tobacco. Histological analysis of the transgenic tobacco showed that GUS mainly localized in the leaf epidermis, stem secondary xylem, and root vasculature. These observations closely correlated with the previously reported presence of GbMVD transcripts in the roots, stems, and leaves. Further functional characterizations in transiently transformed tobacco leaves allowed us to identify the region that can be considered as the minimal promoter. Light and hormonal signals (i.e., ethephon, salicylic acid, and methyl jasmonate) induced the activity of GbMVDpro-driven GUS in transgenic tobacco and enhanced the transcription of GbMVD as well as TTL production in ginkgo seedlings. These results, together with the bioinformatic analysis of GbMVDpro, suggest that the GbMVD promoter harbored a numerous TTL biosynthesis-related cis-elements that regulate the flux of terpenoid precursors.
    Full-text · Article · Oct 2015 · Plant Molecular Biology Reporter
  • Xiaohua Huang · Mei Mei · Chanjuan Liu · Xianglin Pei · Chun Wei
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    ABSTRACT: A novel aromatic diamine monomer, 3,3′-diisopropyl-4,4′-diaminodiphenyl-3′′,4′′-difluorophenylmethane (PAFM), was successfully synthesized by coupling of 2-isopropylaniline and 3,4-difluorobenzaldehyde. The aromatic diamine was adopted to synthesize a series of fluorinated polyimides by polycondensation with various dianhydrides: pyromellitic dianhydride (PMDA), 3,3′,4,4′-biphenyltetracarboxylic dianhydride (BPDA), 4,4′-oxydiphthalic anhydride (ODPA) and 3,3′,4,4′-benzophenone tetracarboxylic dianhydride (BTDA) via the conventional one-step method. These polyimides presented excellent solubility in common organic solvents, such as N,N-dimethylformamide (DMF), N,N-dimethyl acetamide (DMAc), dimethyl sulfoxide (DMSO), N-methyl-2-pyrrolidone (NMP), chloroform (CHCl3), tetrahydrofuran (THF) and so on. The glass transition temperatures (Tg) of fluorinated polyimides were in the range of 260–306°C and the temperature at 10% weight loss in the range of 474–502°C. Their films showed the cut-off wavelengths of 330–361 nm and higher than 80% transparency in a wavelength range of 385–463 nm. Moreover, polymer films exhibited low dielectric properties in the range of 2.76–2.96 at 1 MHz, as well as prominent mechanical properties with tensile strengths of 66.7–97.4 MPa, a tensile modulus of 1.7–2.1 GPa and elongation at break of 7.2%–12.9%. The polymer films also showed outstanding hydrophobicity with the contact angle in the range of 91.2°–97.9°.
    No preview · Article · Sep 2015 · Chinese Journal of Chemistry
  • Chong Sun · Yanlian Niu · Xujie Yang · Min Liu · Xiaodi Yang · Xiaohua Huang · Wenbo Zhao
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    ABSTRACT: In this paper, the novel poly(lactic-co-glycolic acid)-F127 nanospheres (PLGA-F127 NSs) were synthesized and used to establish an amperometric glucose biosensor that can be applied in whole blood directly. This property of glucose biosensor was based on the antibiofouling property of PLGAF127 NSs. More details of preparing PLGA-F127 NSs and immobilizing glucose oxidase (GOx) on (PLGA-F127)/glass carbon electrode (GCE) were presented. Then, the electrochemical behaviors of the biosensor in whole blood were studied. The cyclic voltammetric results indicated that GOx immobilized on PLGA-F127 NSs exhibited direct electron transfer reaction, which led to stable amperometric biosensing for glucose with a detection limit of 5.57×10−6 M (S/N = 3). The glucose biosensor did not respond to ascorbic acid (AA) and uric acid (UA) at their concentration normally encountered in blood. The development of materials science will bring significant input to high-performance biosensors relevant to diagnostics and therapy of interest for human health.
    No preview · Article · Sep 2015 · Journal of Nanoscience and Nanotechnology
  • Xiaohua Huang · Mei Mei · Chanjuan Liu · Xianglin Pei · Chun Wei
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    ABSTRACT: A series of novel organo-soluble and optical transparent polyimides were synthesized via conventional one-step polycondensation from a novel diamine, 3,3′-diterbutyl-4,4′-diaminodiphenyl-4″-morpholinophenylmethane (TAMPM) and various commercial aromatic dianhydrides. The structures of monomer and polymers were confirmed by FT-IR and NMR. The solubility, thermal stability, optical transparency, dielectric and hydrophobic properties of polyimides were investigated. The results showed polymers presented excellent solubility in common organic solvents, such as N,N-dimethylformamide (DMF), N,N-dimethyl acetamide (DMAc), dimethyl sulfoxide (DMSO), N-methyl-2-pyrrolidone (NMP), chloroform (CHCl3), tetrahydrofuran (THF) and dichloromethane (CH2Cl2). The polymers exhibited prominent thermal stability and high optical transparency with the glass transition temperature (T g) in the range of 311–355 °C, and the cutoff wavelengths in the range of 287–344 nm. Furthermore, all of the polymers also presented low dielectric constants in the range of 2.85–3.16 at 1 MHz, and outstanding hydrophobic properties with the contact angle in the rang of 85.1–94.2 o.
    No preview · Article · Sep 2015 · Journal of Polymer Research
  • Source
    Xuanbo Zhang · Yuping Du · Lihong Wang · Qing Zhou · Xiaohua Huang · Zhaoguo Sun
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    ABSTRACT: Rare earth element pollution (REEs) and acid rain (AR) pollution simultaneously occur in many regions, which resulted in a new environmental issue, the combined pollution of REEs and AR. The effects of the combined pollution on the antioxidant enzyme system of plant roots have not been reported. Here, the combined effects of lanthanum ion (La3+), one type of REE, and AR on the antioxidant enzyme system of soybean roots were investigated. In the combined treatment of La3+ (0.08 mM) and AR, the cell membrane permeability and the peroxidation of cell membrane lipid of soybean roots increased, and the superoxide dismutase, catalase, peroxidase and reduced ascorbic acid served as scavengers of reactive oxygen species. In other combined treatments of La3+ (0.40 mM, 1.20 mM) and AR, the membrane permeability, malonyldialdehyde content, superoxide dismutase activity, peroxidase activity and reduced ascorbic acid content increased, while the catalase activity decreased. The increased superoxide dismutase activity, peroxidase activity and reduced ascorbic acid content were inadequate to scavenge the excess hydrogen peroxide and superoxide, leading to the damage of the cell membrane, which was aggravated with the increase in the concentration of La3+ and the level of AR. The deleterious effects of the combined treatment of La3+ and AR were stronger than those of the single treatment of La3+ or AR. Moreover, the activity of antioxidant enzyme system in the combined treatment group was affected directly and indirectly by mineral element content in soybean plants.
    Preview · Article · Aug 2015 · PLoS ONE
  • Juan Li · Hangqing Xie · Yuhong Liu · Hang Ren · Wenbo Zhao · Xiaohua Huang
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    ABSTRACT: Abstract Nanomaterial-based signal-amplification strategies hold a great promise in realizing sensitive biological detection. A simple label-free electrochemical immunosensor for sensitive detection of carcinoembryonic antigen (CEA) was developed by immobilizing anti-CEA antibodies onto the Au-F127 strawberry-like nanospheres modified glassy carbon electrode (Au-F127/GCE). The Au-F127 strawberry-like nanospheres offered a large surface and multifunctional substrate for the effective immobilization of anti-CEA and the existence of Au could accelerate electron transfer and make the electrochemical signal amplified. The Au-F127 nanocomposites and anti-CEA were characterized by transmission electron microscopy (TEM), polycrystalline electron diffraction ring pattern, ultra-violet visible (UV-vis) spectra and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectra. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were employed to verify the stepwise assembly of the immunosensor and evaluated the analytical performance of the fabricated immunosensor, respectively. The immunosensor showed a wide liner response range between 0.01 and 80 ng mL-1 with a low detection limit of 0.24 pg mL-1 at a signal-to-noise (S/N) ratio of 3. Additionally, the proposed method was successfully applied to determine CEA in human serum samples with satisfactory results.
    No preview · Article · Jul 2015 · Talanta
  • Jiazhi Zhang · Lihong Wang · Man Li · Liya Jiao · Qing Zhou · Xiaohua Huang
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    ABSTRACT: The aim of this study was to evaluate the effects of bisphenol A (BPA) on plant photosynthesis and determine whether the photosynthetic response to BPA exposure varies in different plants. Chlorophyll fluorescence techniques were used to investigate the effects of BPA on chlorophyll fluorescence parameters in tomato (Lycopersicum esculentum), lettuce (Lactuca sativa), soybean (Glycine max), maize (Zea mays), and rice (Oryza sativa) seedlings. Low-dose (1.5 or 3.0 mg L(-1)) BPA exposure improved photosystem II efficiency, increased the absorption and conversion efficiency of primary light energy, and accelerated photosynthetic electron transport in each plant, all of which increased photosynthesis. These effects weakened or disappeared after the withdrawal of BPA. High-dose (10.0 mg L(-1)) BPA exposure damaged the photosystem II reaction center, inhibited the photochemical reaction, and caused excess energy to be released as heat. These effects were more evident after the highest BPA dose (17.2 mg L(-1)), but they weakened after the withdrawal of BPA. The magnitude of BPA exposure effects on the chlorophyll fluorescence parameters in the five plants followed the order: lettuce > tomato > soybean > maize > rice. The opposite order was observed following the removal of BPA. In conclusion, the chlorophyll fluorescence response in plants exposed to BPA depended on BPA dose and plant species.
    No preview · Article · Jul 2015 · Environmental Science and Pollution Research
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    ABSTRACT: Bisphenol A (BPA) is a ubiquitous endocrine-disrupting chemical in the environment that exerts potential harm to plants. Phytohormones play important roles both in regulating multiple aspects of plant growth and in plants' responses to environmental stresses. But how BPA affects plant growth by regulating endogenous hormones remains poorly understood. Here, we found that treatment with 1.5 mg L(-1) BPA improved the growth of soybean seedlings, companied by increases in the contents of indole-3-acetic acid (IAA) and zeatin (ZT), and decreases in the ratios of abscisic acid (ABA)/IAA, ABA/gibberellic acid (GA), ABA/ZT, ethylene (ETH)/GA, ETH/IAA, and ETH/ZT. Treatment with higher concentrations of BPA (from 3 to 96 mg L(-1)) inhibited the growth of soybean seedlings, meanwhile, decreased the contents of IAA, GA, ZT, and ETH, and increased the content of ABA and the ratios of ABA/IAA, ABA/GA, ABA/ZT, ETH/GA, ETH/IAA, and ETH/ZT. The increases in the ratios of growth and stress hormones were correlated with the increase in the BPA content of the roots. Thus, BPA could affect plant growth through changing the levels of single endogenous hormone and the ratios of growth and stress hormones in the roots because of BPA absorption by the roots.
    No preview · Article · Jul 2015 · Environmental Science and Pollution Research
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    ABSTRACT: The terpene trilactones (TTLs) are believed to be important for the pharmacological properties of Ginkgo biloba leaves extract. 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is a critical enzyme involved in the biosynthetic pathway of TTLs. In this study, an 1.2-kb fragment of 5' flanking region of the HMGR gene (GbHMGR), was isolated from G. biloba by genome walking. Extensive sequence analysis revealed the presence of evolutionarily conserved and over-represented putative cis-acting elements in light-regulated transcription, hormone signaling (gibberellic acid, jasmonate and salicylic acid), elicitor and stress responses (cold/dehydration responses), and plant defense signaling (W-box/WRKY) that are common to the promoter region of GbHMGR. EMSA analysis suggested possible functionality of W-box in GbHMGR promoter region. The behavior of gene transcripts in ginkgo callus upon light, low temperature, MeJA and SA treatments further verified the regulatory function of GbHMGR promoter. A significant positive relationship between gene expression level and total TTL contents suggested that GbHMGR might be one of key genes involved in TTL biosynthesis in G. biloba.
    Full-text · Article · May 2015 · Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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    ABSTRACT: Environmental endocrine disrupter-bisphenol A (BPA) is ubiquitous in the environment, with potential toxic effects on plants. Previous studies found a significant effect of BPA on levels of mineral nutrients in plant roots, but the underlying mechanism remains unknown. To determine how BPA influences root mineral nutrients, the effects of BPA (1.5, 3.0, 6.0, 12.0, 24.0, 48.0 and 96.0 mg L(-1) ) on activities of critical respiratory enzymes (hexokinase, phosphofructokinase, pyruvate kinase, isocitrate dehydrogenase and cytochrome c oxidase) were investigated in soybean seedling roots. After BPA exposure for 7 d, the low concentrations of BPA increased the activities of critical respiratory enzymes in roots while the opposite effects were observed in roots exposed to high concentrations of BPA, and the inhibitory effect was greater for higher BPA concentrations. Meanwhile, evident morphological anomalies and decreases in root lengths and volumes were induced by high concentrations of BPA. Following withdrawal of BPA exposure for 7 d, the activities of respiratory enzymes and visible signs of toxicity recovered, and the extent of recovery depended on the type of enzyme and the BPA concentration. Furthermore, correlation analysis showed that the disturbance by BPA to activities of respiratory enzymes, which led to interference in the energy metabolism in roots, may be effect mechanism of BPA on mineral element accumulation in plant roots. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    No preview · Article · May 2015 · Environmental Toxicology and Chemistry
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    ABSTRACT: Bisphenol A (BPA), an emerging pollutant in the environment, with potential toxic effects on plants; however, the toxicity mechanism remains largely unknown. The antioxidant system plays an important role in protecting plants against the damage of stress. Here, the effects of BPA on the antioxidant system [superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbic acid (AsA), proline, reduced glutathione (GSH)], reactive oxygen species [ROS, hydrogen peroxide (H2 O2 ), superoxide anion (O2 (-) )] accumulation and membrane lipid peroxidation [malondialdehyde (MDA), cell membrane permeability] in soybean seedling roots were investigated. The 1.5 mg L(-1) BPA exposure didn't affect test indices in the roots. The exposure to 3.0, 6.0, 12.0 or 24.0 mg L(-1) BPA caused increases in the SOD (except for 3.0 mg L(-1) BPA) and CAT activities as well as the AsA, proline and GSH (except for 3.0 mg L(-1) BPA) contents, leading to increases in the H2 O2 and O2 (-) contents and to membrane lipid peroxidation. The 48.0 or 96.0 mg L(-1) exposure BPA caused decreases in the CAT activity and AsA/GSH content as well as increases in the SOD and POD activities and the proline content, leading to excess ROS accumulation (i.e. H2 O2 and O2 (-) ) and cell membrane damage. After withdrawal of BPA exposure, ROS accumulation and membrane lipid peroxidation were alleviated through regulating special antioxidant enzyme or substance. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    No preview · Article · Feb 2015 · Environmental Toxicology and Chemistry
  • Qing Yang · Lihong Wang · Qing Zhou · Xiaohua Huang
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    ABSTRACT: The environmental safety of rare earth elements (REEs), especially the toxic effect of REEs on plants, has attracted increasing attention. However, the cellular mechanism of this toxic effect remains largely unknown. Here, the toxic effects of heavy REE terbium ion [Tb(III)] on the cell membrane of horseradish roots were investigated by using electron microscope autoradiography (EMARG) and histochemical methods. The results indicated that Tb(III) was distributed in the extracellular and intracellular spaces of the roots after horseradish was treated with Tb(III). Moreover, the percentage contents of the unsaturated fatty acids in the membrane lipids, the current of the outward K+ channel and the average diameter of membrane proteins in the roots of horseradish treated with Tb(III) were decreased; on the contrary, the percentage contents of the saturated fatty acids and malondialdehyde in the roots of horseradish treated with Tb(III) were increased. Furthermore, the contents of intracellular N, P, Mg and Fe in the roots of horseradish treated with Tb(III) were decreased, while the contents of intracellular K and Ca in the roots of horseradish treated with Tb(III) were increased. Finally, the effects of Tb(III) on horseradish roots were increased with increasing concentration or duration of Tb(III) treatment. In conclusion, after horseradish was treated with Tb(III), Tb(III) could enter the cells of horseradish roots and lead to the toxic effects on horseradish, which caused the oxidation of the unsaturated fatty acids in the membrane lipids, the changes in the membrane proteins (including the outward K+ channel), the decrease in the membrane fluidity, and then the inhibition of the intracellular/extracellular-ion exchange in horseradish roots.
    No preview · Article · Jan 2015 · Ecotoxicology and Environmental Safety
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    ABSTRACT: As a novel class of dynamic and non-covalent polymers, supramolecular polymers not only display specific structural and physicochemical properties, but also have the ability to undergo reversible changes of structure, shape, and function in response to diverse external stimuli, making them promising candidates for widespread applications ranging from academic research to industrial fields. By an elegant combination of dynamic/reversible structures with exceptional functions, functional supramolecular polymers are attracting increasing attention in various fields. In particular, functional supramolecular polymers offer several unique advantages, including inherent degradable polymer backbones, smart responsiveness to various biological stimuli, and the ease for the incorporation of multiple biofunctionalities (e.g., targeting and bioactivity), thereby showing great potential for a wide range of applications in the biomedical field. In this Review, the trends and representative achievements in the design and synthesis of supramolecular polymers with specific functions are summarized, as well as their wide-ranging biomedical applications such as drug delivery, gene transfection, protein delivery, bio-imaging and diagnosis, tissue engineering, and biomimetic chemistry. These achievements further inspire persistent efforts in an emerging interdisciplin-ary research area of supramolecular chemistry, polymer science, material science, biomedical engineering, and nanotechnology.
    No preview · Article · Jan 2015 · Advanced Materials
  • Chanjuan Liu · Xianglin Pei · Xiaohua Huang · Chun Wei · Xiaoyi Sun
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    ABSTRACT: A novel aromatic diamine monomer bearing tertbutyl and 4-tertbutylphenyl groups, 3,3′-ditertbutyl-4,4′-diaminodiphenyl-4′′-tertbutylphenylmethane (TADBP), was prepared and characterized. A series of non-coplanar polyimides (PIs) were synthesized via a conventional one-step polycondensation from TADBP and various aromatic dianhydrides including pyromellitic dianhydride (PMDA), 3,3′,4,4′-biphenyltetracarboxylic dianhydride (BPDA), 4,4′-oxydiphthalic anhydride (OPDA), 3,3′,4,4′-benzophenone tetracarboxylic dianhydride (BTDA) and 4,4′-(hexafluoroisopropylidene)dipthalic anhydride (6FDA). All PIs exhibit excellent solubility in common organic solvents such as N,N-dimethylformamide (DMF), N,N-dimethylacetamide (DMAc), N-methyl-2-pyrrolidone (NMP), dimethyl sulfoxide (DMSO), chloroform (CHCl3), tetrahydrofuran (THF), and so on. Furthermore, the obtained transparent, strong and flexible polyimide films present good thermal stability and outstanding optical properties. Their glass transition temperatures (Tgs) are in the range of 298 to 347°C, and 10% weight loss temperatures are in excess of 490°C with more than 53% char yield at 800°C in nitrogen. All the polyimides can be cast into transparent and flexible films with tensile strength of 80.5–101 MPa, elongation at break of 8.4%–10.5%, and Young's modulus of 2.3–2.8 GPa. Meanwhile, the PIs show the cutoff wavelengths of 302–356 nm, as well as low moisture absorption (0.30% –0.55%) and low dielectric constant (2.78–3.12 at 1 MHz).
    No preview · Article · Jan 2015 · Chinese Journal of Chemistry

Publication Stats

938 Citations
340.67 Total Impact Points

Institutions

  • 2006-2016
    • Nanjing Normal University
      • • College of Chemistry and Materials Science
      • • College of Chemistry and Environmental Science
      • • Department of Chemistry
      Nan-ching, Jiangsu, China
  • 2015
    • Huanggang Normal University
      Ng kng, Guangdong, China
  • 2013-2015
    • Guilin University of Technology
      Ling-ch’uan, Guangxi Zhuangzu Zizhiqu, China
  • 2014
    • Jiangnan University
      • School of Environmental and Civil Engineering
      Wu-hsi, Jiangsu Sheng, China
  • 2011-2014
    • Shanghai Jiao Tong University
      • State Key Laboratory of Metal Matrix Composites
      Shanghai, Shanghai Shi, China
  • 2003-2012
    • Yangtze University
      Hu-pei-ts’un, Shanxi Sheng, China