Li-Ping Jiang

Nanjing University, Nan-ching, Jiangsu Sheng, China

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Publications (53)262.15 Total impact

  • Jie Ding · Ying Zhou · Jingjing Li · Li-Ping Jiang · Zhiwei He · Jun-Jie Zhu
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    ABSTRACT: The high-performance and noninvasive screening of heterogeneous tumor subtypes in vivo is particularly desirable for the diagnosis and symptomatic treatment of cancer. Therefore, we report a near-infrared (NIR) fluorescence tracer "smartly identified HER2" (SI-HER2) for rapid, accurate and highly-specific screening of HER2 overexpressed breast cancer. An antibody against HER2 protein receptor, EP1045Y, was conjugated with NIR emitting CdSeTe/CdS/ZnS QDs via polyhistidine-driven self-assembly approach. The further adsorption of black hole quencher 3 on antibody enabled a "turn on" fluorescence response of the fluorescence tracer to HER2 protein receptor. Aside from the capability of differentiating the HER2 overexpressed MCF-7 cells from its counterparts, the fluorescence tracer can also accurately and rapidly identify the HER2 overexpressed breast tumor subtype in two tumors-bearing mouse model, providing a platform for the investigation of advanced pathways to distinguish the different breast cancer subtypes.
    No preview · Article · Nov 2015 · Analytical Chemistry
  • Shanhu Liu · Ziyi Zhang · Shiwei Zhou · Li-Ping Jiang · Jun-Jie Zhu
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    ABSTRACT: An electrochemical-TUNEL method was developed for a cell sensor. A 3-D bio-interface based on CNT@PDA-FA was employed in the cytosensor, which significantly improved the cell capture. By coupling with a QD-based nanoprobe and electrochemical stripping analysis, the cytosensor exhibited attractive performance for detection of apoptotic cells.
    No preview · Article · Oct 2015 · The Analyst
  • Juan Peng · Jufang Guan · Li-Ping Jiang · Jun-Jie Zhu
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    ABSTRACT: Luminescent graphene quantum dots (GQDs) have generated enormous excitement because of their superiority in chemical inertness, biocompatibility and low toxicity. In addition, due to their unique structure, GQDs exhibit the excellent performances of graphene and other special physical properties. Researches on GQDs in aspects of chemistry, physics, material and biology have sprung up rapidly in the past few years. In this review, recent developments in properties and biological applications of GQDs are summarized. An outlook is given to their future and potential development involving the luminescent mechanism, purify, and the potential applications in biological fields.
    No preview · Article · Oct 2015 · Science of Advanced Materials
  • Jie Ding · Jing Yao · Jingjing Xue · Rong Li · Bo Bao · Li-Ping Jiang · Jun-Jie Zhu · Zhiwei He
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    ABSTRACT: Chemotherapy is the use of chemical drugs to prevent cancer cell proliferation, invasion and metastasis, but serious obstacle is that chemotherapeutics strikes not only on cancerous cells, but also on normal cells. Thus, anticancer drugs without side effects should been developed and extracted. (-)-Epigallocatechin-3-gallate (EGCG), a major ingredient of green tea, possesses excellent medicinal values, such as anticancer effects, DNA-protective effects, etc. However, EGCG will be mostly metabolized if it is directly took by oral ingestion. Here, we report a drug delivery system (DDS) for loading EGCG to enhance its stability, promising target and anticancer effect in vitro and in vivo. The designed DDS is composed of three main moieties: anticancer drug, EGCG; drug vector, colloidal mesoporous silica (CMS); target ligand, breast tumor-homing cell-penetrating peptide (PEGA-pVEC peptide). Based on the results of cck-8 assay, confocal imaging, cell cycle analysis and western blot, the anticancer effect of EGCG was increased by loading of EGCG into CMS and CMS@peptide. In vivo treatment displayed that CMS had not obvious influence on breast tumor bearing mice, but CMS@peptide@EGCG showed the greatest tumor inhibition rate with about 89.66%. H&E staining of organs showed no tissue injure in all experimental groups. All above results prove that EGCG is an excellent anticancer drug without side effect and CMS@peptide could greatly promote the efficacy of EGCG on breast tumor by targeted accumulation and release, which provide much evidence for the CMS@peptide as a promising and targeting vector for DDS.
    No preview · Article · Jul 2015 · ACS Applied Materials & Interfaces
  • Qin Xu · YuanYuan Shen · JiaQian Tang · Min-Hua Xue · Li-Ping Jiang · Xiaoya Hu
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    ABSTRACT: A simple cyclic voltammetric method has been applied to assemble and orient a model protein, Myoglobin (Mb), into a biocompatible Brij 56 film. Ultra-visible (UV-vis) and circular dichroism (CD) spectra indicated that Mb in Brij 56 matrix preserved its secondary structure. Fourier transform infrared spectra (FT-IR) confirmed the formation of hydrogen bonds between Mb and Brij 56. These hydrogen bonds were acted as the electron tunnel to transfer electrons from Mb's active sites to the underlying glassy carbon electrode. Effective direct electron transfer (DET) of Mb was realized with the presence of a couple of quasi-reversible and well-defined redox peaks at -310 mV (vs. SCE) in the studied potential range. The peaks were attributed to the redox couple of heme Fe(II)/ Fe(III) of the well-oriented Mb in Brij 56 matrix. The surface coverage and the electron transfer rate (ks) of Mb immobilized into the Brij 56 film was about 4.9×10-11 mol cm-2 and 72.6 3.0 s-1, respectively. An excellent electrocatalytic response of the immobilized Mb towards nitrite in the absence of electron transfer mediators was observed. These results emphasized that the biomimetic Brij 56 could be used as an attractive material for immobilizing proteins and constructing biosensors.
    No preview · Article · May 2015 · ACS Applied Materials & Interfaces
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    ABSTRACT: A novel "signal-on" photoelectrochemical (PEC) biosensor for sensitive detection of HTLV-II DNA was developed based on enzymatic amplification coupled with terminal deoxynucleotidyl transferase (TdT)-mediated extension strategy. The intensity of the photocurrent signal was proportional to the concentration of the HTLV-II DNA-target DNA (tDNA) by dual signal amplification. In this protocol, GR-CdS:Mn/ZnS nanocomposites were used as photoelectric conversion material, while pDNA was used as the tDNA recognizing unit. Moreover, the TdT-mediated extension and the enzymatic signal amplification technique were used to enhance the sensitivity of detection. Using this novel "signal-on" dual signal amplification strategy, the prototype of PEC DNA sensor can detect as low as sub-femtomolar HTLV-II DNA with excellent differentiation ability even for single-base mismatches. This PEC DNA assay opens a promising platform to detect various DNA targets at ultra-low levels for early diagnoses of different diseases.
    No preview · Article · Apr 2015 · Analytical Chemistry
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    ABSTRACT: Global characterization and in-depth understanding of phosphoproteome based on mass spectrometry (MS) desperately needs a highly efficient affinity probe during sample preparation. In this work, a ternary nanocomposite of magnetite/ceria co-decorated titanoniobate nanosheet (MC-TiNbNS) was synthesized by electrostatic assembly of Fe3O4 nanospheres and in situ growth of CeO2 nanoparticles on pre-exfoliated titanoniobate, and eventually utilized as the probe and catalyst for enrichment and dephosphorylation of phosphopeptides. The two-dimensional (2D) structured titanoniobate nanosheet not only promoted the efficacy of capturing phosphopeptides with enlarged surface area, but also functioned as a substrate for embracing magnetic anchor Fe3O4 to enable magnetic separation and mimic phosphatase CeO2 to produce identifying signatures of phosphopeptides. Compared to single component TiNbNS or CeO2 nanoparticles, the ternary nanocomposite provided direct evidence on the number of phosphorylation sites while maintaining the enrichment efficiency. Moreover, by altering on-sheet CeO2 coverage, the dephosphorylation activity could be fine tuned, generating continuously adjustable signal intensities of both phosphopeptides and their dephosphorylated tags. Exhaustive detection of both mono- and multi-phosphorylated peptides with precise counting of their phosphorylation sites were achieved in the primary mass spectra in the cases of digests of standard phosphoprotein and skim milk, as well as more complex biological sample, human serum. With the resulting highly informative mass spectra, this multifunctional probe can be used as a promising tool for fast and comprehensive characterization of phosphopeptides in MS-based phosphoproteomics.
    No preview · Article · Mar 2015 · ACS Applied Materials & Interfaces
  • Hongying Liu · Zhimei He · Li-Ping Jiang · Jun-Jie Zhu
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    ABSTRACT: A facile and rapid strategy was developed for the synthesis of ultrabright luminescent carbon nanodots (CDs) with tunable wavelength from 464 to 556 nm by introducing glutaraldehyde into the solution under microwave irradiation. The fluorescence properties, including excitation and emission wavelength, quantum yield (QY), and size of the CDs, were adjusted by changing the amount of glutaraldehyde and poly (ethylenimine). Several methods such as UV-Vis, fluorescence, and Fourier transform infrared (FTIR) spectroscopy, high resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), and dynamic light scattering (DLS) were used to characterize the morphology and the properties of the CDs. And the luminescence mechanism was also discussed. In addition, confocal microscopy imaging revealed that the as-prepared CDs could be used as effective fluorescent probes in the imaging of HeLa cells without obvious cytotoxicity. Furthermore, a novel sensor for the detection of Co2+ was proposed on the basis of Co2+-induced fluorescence quenching. These superior properties demonstrated the potential applications of the CDs in cellular imaging and ion sensing.
    No preview · Article · Feb 2015 · ACS Applied Materials & Interfaces
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    ABSTRACT: MicroRNAs(miRNAs) have emerged as new candidate diagnostic and prognostic biomarkers for the detection of a wide variety of cancers, thus sensitive and selective detection of microRNAs is significant for early-phase cancers diagnosis and disease prevention. A novel and simple electrochemical miRNAs biosensor was developed using Cd2+ modified titanium phosphate nanoparticles as signal unit, two DNA as capture probes and Ru(NH3)63+ as electron transfer mediator. Large quantities of cadmium ions were mounted in titanium phosphate spheres to output the electrochemical signal. Because of the presence of Ru(NH3)63+ molecules which interacted with DNA base-pairs as electron wire, the electrochemical signal significantly increased more than 5 times. This approach achieved a wide dynamic linear range from 1.0 aM to 10.0 pM with an ultra-low limit detection of 0.76 aM, exerting a substantial enhancement in sensitivity. Moreover, the proposed biosensor was sufficiently selective to discriminate the target miRNAs from homologous miRNAs and could be used for rapid and direct analysis of miRNAs in human serum. Therefore, this strategy provides a new and ultrasensitive platform for miRNA expression profiling in biomedical research and clinical diagnosis.
    No preview · Article · Jan 2015 · ACS Applied Materials & Interfaces
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    ABSTRACT: Upconversion nanophosphors (UCNPs) are extremely useful for analytical applications, since they display a high signal-to-noise ratio, and the photobleaching can be ignored. Herein, a novel upconversion nanocomposite composed of β-cyclodextrin (β-CD) derivative modified UCNPs and rhodamine B (RB),was prepared for the detection of cholesterol (Cho). The upconversion luminescence (UCL) emission can serve as a Cho-sensing signal by an effective fluorescence resonance energy transfer (FRET) process, using UCNPs as the donor and RB as the quencher. The sensor for Cho detection in human serum shows excellent sensitivity and selectivity, which has the potential for clinical applications in the analysis of other biological and environmental samples.
    No preview · Article · Oct 2014 · Nanoscale
  • Li-Min Liu · Guo-Yuan Lu · Li-Ping Jiang · Jun-Jie Zhu
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    ABSTRACT: An aminoethoxy-functionalized zirconium phosphonate (Zr(O3POCH2CH2NH2)(2)center dot 3H(2)O), abbreviated as ZrRP (R=OCH2CH2NH2); with layered structure has been synthesized. This layered compound possesses the characteristic of inorganic-organic hybrid, due to the covalently linked aminoethoxy in the host layer. The anion exchanged property of this zirconium phosphonate is suitable for the direct intercalation of negatively charged DNA, which is different from these reported zirconium phosphates or zirconium phosphonates. As a precursor, this prepared zirconium phosphonate was utilized to fabricate a novel DNA/ZrRP binary hybrid via a delamination-reassembly procedure. The release behavior of DNA from the DNA/ZrRP composite was investigated at different medium pH, because the combination between zirconium phosphonate sheets and DNA was pH-dependent sensitively. Moreover, the helical conformation of DNA was almost retained after the intercalation and release process. These properties of the DNA/ZrRP composite suggested the potential application of layered zirconium phosphonate as a non-viral vector in gene delivery.
    No preview · Article · Jul 2014 · Journal of Solid State Chemistry
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    ABSTRACT: A surprising electrochemiluminescence (ECL) enhancement effect in graded-gap CdSeTe@ZnS-SiO2 quantum dot (QD) bilayers was observed and used to create an ultrasensitive immunoassay. CdSeTe@ZnS-SiO2 QDs of two different sizes were used as a donor-acceptor pair, owing to their tunable energy and low biotoxicity. The graded-gap CdSeTe@ZnS-SiO2 QD bilayers were fabricated by layer-by-layer assembly of differently sized CdSeTe@ZnS-SiO2 QDs on a glutaraldehyde-activated electrode. Benefiting from a short interlayer distance and perfect spectral overlap in the graded-gap QD bilayers, highly efficient ECL resonance energy transfer (ECLRET)-based energy funneling was observed, wherein excitons from trapped states could be effectively recycled. Consequently, the observed ECL enhancement for the bilayers was more than four times greater than that observed for reference samples. The graded-gap QD bilayers were utilized in an ECL biosensor for the detection of carcinoembryonic antigen (CEA). The proposed method featured a detection limit of 0.4 pg mL(-1) CEA with a linear calibration range from 1 pg mL(-1) to 200 ng mL(-1). This method represents a novel approach for versatile detection of biomolecules in research and clinical applications.
    No preview · Article · Mar 2014 · Analytical Chemistry
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    ABSTRACT: We report on a sensitive electrochemical immunoassay for the prostate specific antigen (PSA). An immunoelectrode was fabricated by coating a glassy carbon electrode with multiwalled carbon nanotubes, poly(dimethyldiallylammonium chloride), CeO2 and PSA antibody (in this order) using the layer-by-layer method. The immunosensor is then placed in a sample solution containing PSA and o-phenylenediamine (OPD). It is found that the CeO2 nanoparticles facilitate the electrochemical oxidation of OPD, and this produces a signal for electrochemical detection of PSA that depends on the concentration of PSA. There is a linear relationship between the decrease in current and the concentration of PSA in the 0.01 to 1,000 pg mL−1 concentration range, and the detection limit is 4 fg mL−1. The assay was successfully applied to the detection of PSA in serum samples. This new differential pulse voltammetric immunoassay is sensitive and acceptably precise, and the fabrication of the electrode is well reproducible. Figure A novel electrochemical immunoassay for prostate specific antigen (PSA) was developed. Ceria (CeO2) mesoporous nanospheres facilitated the electrochemical oxidation of o-phenylenediamine (OPD). The developed immunoassay has high sensitivity and can be successfully applied for the detection of PSA in serum samples
    No preview · Article · Mar 2014 · Microchimica Acta
  • Source
    Ying-Di Zhu · Juan Peng · Li-Ping Jiang · Jun-Jie Zhu
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    ABSTRACT: A novel fluorescent immunosensor was developed based on the use of CuS nanoparticles (CuS NPs) as labels for the highly sensitive detection of human prostate cancer biomarker prostate specific antigen (PSA). In the presence of CuS NPs, the non-fluorescent substrate o-phenylenediamine could be oxidized into the stable fluorescent product 2,3-diamiophenazine at physiological pH. Throughout the reaction, no other oxidizing agents (e.g. hydrogen peroxide) were needed. The relatively mild oxidation conditions made the immunoassay robust, reliable and facile. The proposed immunoassay exhibited high sensitivity and specificity for the detection of PSA. A linear relationship between the fluorescent signals and the concentration of PSA was obtained in the range of 0.5 pg mL(-1) to 50 ng mL(-1), with a detection limit of 0.1 pg mL(-1) (S/N = 3). The proposed fluorescent immunoassay can be used as a promising platform for the detection of a variety of other biomarkers.
    Full-text · Article · Dec 2013 · The Analyst
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    ABSTRACT: A multifunctional boron nitride-gold nanocluster composite was fabricated using poly-diallyldimethylammonium chloride as a stabilizer and a linker. The as-fabricated composite could be used as a fluorescent or an electrochemical label for immunosensing in the sensitive detection of interleukin-6.
    No preview · Article · Oct 2013 · Chemical Communications
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    ABSTRACT: Graphene quantum dots (GQDs) with green fluorescence were incorporated onto hexagonal boron nitride sheets (HBN) through electrostatic interaction by using poly(diallyldimethylammonium) (PDDA) as the bridge to fabricate the novel nanocomposites (HBN-GQDs). The HBN-GQDs nanocomposites exhibited strong green fluorescent property, high stability, water solubility, very low cytotoxicity on Hela cells. These properties make the HBN-GQDs nanocomposites as good candidate materials for biological applications. The results for the imaging of live cells indicated that the cell-penetrating HBN-GQDs could be a promising nanoprobe for intracellular imaging and therapeutic applications.
    Full-text · Article · Oct 2013 · Journal of Biomedical Nanotechnology
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    ABSTRACT: In this paper, hemoglobin/DNA/layered double hydroxide composites were fabricated by co-intercalating hemoglobin (Hb) and DNA in the interlayer galleries of Ni–Al layered double hydroxides (Ni–Al LDH) via a delamination-reassembly procedure. The presence of DNA not only remarkably facilitated the intercalation of Hb into the interlayers of the Ni–Al LDH, but also improved the bioactivity of the entrapped Hb. By depositing the Hb/DNA/LDH composite film onto a glassy carbon electrode, a mediator-free biosensor was obtained. Because of the synergistic effect between the LDH host and the co-intercalated DNA guest, the Hb/DNA/LDH composites modified electrode exhibited a fast direct electron transfer with a rate constant, ks, of 6.95 ± 3.31 s−1 and a sensitive electrocatalytic response to H2O2 and NO2− in the linear range of 4.85 × 10−7 to 1.94 × 10−4 M and 2.5 × 10−7 to 3.0 × 10−5 M, respectively. Furthermore, with the protective effect provided by the LDH nanosheets, the electrocatalytic response of the intercalated Hb to H2O2 was retained, even at high temperatures (85 °C) or in the presence of an organic solvent (CH3CN).
    No preview · Article · Jun 2013 · Analytical methods
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    ABSTRACT: Haemin-functionalised magnetic iron(II, III) oxide (Fe3O4) nanoparticles (Fe3O4/haemin) were synthesised by changing the acidity of a solution of the two compounds. The nanoparticles were characterised by transmission electron microscopy, UV-vis absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, measurement of magnetisation, and electrochemical techniques. The properties of both haemin and Fe3O4 were retained. Thus, Fe3O4/haemin nanoparticles exhibited pronounced electrocatalytic activity towards trichloroacetic acid (TCA) like haemin itself. Interestingly, electrocatalytic activity towards TCA was affected by detection temperature, which was controlled via electrically heated carbon paste electrodes. The maximal catalytic current was 5.8 times higher at 60°C than at room temperature (25°C). This proposed electrochemical sensor for TCA possessed a linear detection range of 5-80μM and a detection limit of 0.3μM at 60°C.
    No preview · Article · Jun 2013 · Analytica chimica acta
  • Feng Lu · Shanhu Liu · Li-Ping Jiang · Jun-Jie Zhu
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    ABSTRACT: CdSexTe1-x semiconductor alloy nanotubes (with an external diameter of 140 nm and an internal diameter of 100 nm) were successfully prepared based on the sacrificial template of Cd(OH)Cl nanorods, and were further in situ assembled with gold nanoparticles (AuNPs) via a sonochemical approach to form the CdSexTe1−x/Au nanotubes. The prepared CdSexTe1−x/Au nanotubes were characterized by X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, energy-dispersive spectrometry, and X-ray photoelectron spectra. CdSexTe1−x/Au nanotubes could integrate the advantages of the electronic properties of CdSeTe and the biocompatible properties of AuNPs. A novel biosensor was fabricated after the immobilization of hemoglobin (Hb) on CdSexTe1−x/Au nanotubes. The immobilized Hb exhibited fast direct electron transfer and good electrocatalytic performance to H2O2.
    No preview · Article · Jan 2013 · Journal of Nanoparticle Research
  • Shanhu Liu · Feng Lu · Yan Liu · Li-Ping Jiang · Jun-Jie Zhu
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    ABSTRACT: Fabrication of multifunctional nanocomposites that are simultaneously magnetic, stable, and biocompatible is critical for their application in bioanalysis but remains a challenge. In this study, Fe3O4@C–Au nanocomposites were prepared by a rapid microwave-hydrothermal procedure followed by the layer-by-layer assembly for this intention. The morphologies, composition, phase structures, and magnetic properties of the nanocomposites were well-characterized by field emission scanning electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectra, and superconducting quantum interference device. The nanocomposites have the sizes of about 200 nm in diameter. They integrate the advantages of the satisfactory stability of carbon shell, the good biocompatibility and conductivity of Au nanoparticles as well as the excellent magnetic properties of Fe3O4 core. Furthermore, a novel biosensor based on the multifunctional nanocomposites was fabricated after the immobilization of hemoglobin and the direct electrochemistry was studied. The proposed biosensor exhibits wide linear range, high sensitivity, good reproducibility, and stability. The presented multifunctional nanocomposites provide an alternative and effective matrix for the direct electrochemistry of proteins and might have potential applications in biosensing and drug delivery.
    No preview · Article · Dec 2012 · Journal of Nanoparticle Research

Publication Stats

1k Citations
262.15 Total Impact Points


  • 2004-2015
    • Nanjing University
      • • Department of Chemical Engineering
      • • Department of Chemistry
      Nan-ching, Jiangsu Sheng, China
  • 2012
    • Henan University
      • Institute of Environmental and Analytical Sciences
      K’ai-feng-shih, Henan Sheng, China