Wen-Ming Chen

National Kaohsiung Marine University, Kao-hsiung-shih, Kaohsiung, Taiwan

Are you Wen-Ming Chen?

Claim your profile

Publications (188)510.89 Total impact

  • Wen-Ming Chen · Shih-Yi Sheu · Yi-Ling Chen
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain, designated TTM-43T, was isolated from a water sample taken from the Caohu River in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain TTM-43T were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped that were surrounded by a thick capsule and forming bright yellow colored colonies. Growth occurred at 15-30 oC (optimum, 20 °C), at pH 5-9 (optimum, pH 6) and with 0-0.3 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TTM-43T belonged to the genus Flavobacterium and was most closely related to Flavobacterium terrae R2A1-13T with sequence similarity of 97.8 %. Strain TTM-43T contained iso-C15:0 (24.9 %), iso-C15:1 G (12.6 %), summed feature 9 (iso-C17:1 9c and/or 10-methyl C16:0; 12.2 %), iso-C17:0 3-OH (10.9 %), iso-C15:0 3-OH (7.3 %) and iso-C16:0 (6.7 %) as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. Genomic DNA G+C content of strain TTM-43T was 31 mol%. The DNA-DNA relatedness of strain TTM-43T with respect to recognized species of the genus Flavobacterium was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain TTM-43T should be classified as a novel species, for which the name Flavobacterium brevivitae sp. nov. is presented. The type strain is TTM-43T (=BCRC 80913T =KCTC 42744T =LMG 29004T).
    No preview · Article · Jan 2016 · International Journal of Systematic and Evolutionary Microbiology
  • Wen-Ming Chen · Zih-Han Chen · Shih-Yi Sheu
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain designated SLH-16T was isolated from a fish culture pond in Taiwan and characterized using the polyphasic taxonomic approach. Cells of strain SLH-16T were Gram-staining-negative, aerobic, motile, showing rod-shaped that were covered by large capsules and formed yellow-colored colonies. Growth occurred at 20-40 °C (optimum, 37-40 °C), at pH 4.0-9.0 (optimum, pH 5.0-6.0) and with 0-0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SLH-16T belonged to the genus Novosphingobium and was most closely related to Novosphingobium taihuense T3-B9T with sequence similarity of 97.3 %. The major fatty acids (>10 %) of strain SLH-16T were C18:1 7c, summed feature 3 (C16:17c and/or C16:16c) and C16:0. The major 2-hydroxy fatty acid was C14:0 2-OH. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine and several uncharacterized lipids. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 65.2 mol%. The DNA-DNA hybridization value for strain SLH-16T with Novosphingobium taihuense was less than 43.2 %. Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Novosphingobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain SLH-16T represents a novel species in the genus Novosphingobium, for which the name Novosphingobium piscinae sp. nov. is proposed. The type strain is SLH-16T (=BCRC 80888T =LMG 28418T =KCTC 42194T).
    No preview · Article · Jan 2016 · International Journal of Systematic and Evolutionary Microbiology
  • Zih-Han Chen · Wen-Ming Chen · Shih-Yi Sheu · Chiu-Chung Young
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain, designated KBP-30T, was isolated from a water sample taken from the Banping Lake Wetland Park in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain KBP-30T were Gram-stain-negative, aerobic, motile by gliding rods that were covered by large capsules and formed red-colored colonies. Growth occurred at 10-37 oC (optimum, 20 °C), at pH 6-8 (optimum, pH 6) and with 0-1 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KBP-30T belonged to the genus Hymenobacter and was most closely related to Hymenobacter ocellatus Myx 2105T with sequence similarity of 97.7 % and less than 95.1 % with other members of the genus. Strain KBP-30T contained iso-C15:0, summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B), anteiso-C15:0, iso-C17:0 3-OH and summed feature 3 (C16:1 7c and/or C16:1 6c) as the predominant fatty acids. The major isoprenoid quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine and three unidentified aminophospholipids, unidentified aminolipid, unidentified glycolipid and eight unidentified lipids. The major polyamine was homospermidine. The DNA G+C content of the genomic DNA was 60.3 mol%. The DNA-DNA relatedness of strain KBP-30T with respect to Hymenobacter ocellatus Myx 2105T was less than 42 %. On the basis of the phylogenetic inference and phenotypic data, strain KBP-30T should be classified as a novel species, for which the name Hymenobacter paludis sp. nov. is proposed. The type strain is KBP-30T (=LMG 27293T=KCTC 32237T).
    No preview · Article · Jan 2016 · International Journal of Systematic and Evolutionary Microbiology
  • Shih-Yi Sheu · Zih-Han Chen · Chiu-Chung Young · Wen-Ming Chen
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain, designated shin9-1T, was isolated from a water sample taken from the water convoevueus field in Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain shin9-1T were aerobic, Gram-stain-negative, rod-shaped that were surrounded by a thick capsule and formed cream colored colonies. Growth occurred at 10-45 °C (optimum, 30 °C), with 0-3.0 % NaCl (optimum, 0.5 %) and at pH 7.0-9.0 (optimum, pH 7.0). Strain shin9-1T did not form nodules on a legume plant, Macroptilium atropurpureum, and the nodulation genes nodA, nodC and the nitrogenase reductase gene nifH were not detected by PCR. Phylogenetic analyses based on 16S rRNA and three housekeeping gene sequences (recA, atpD and rpoB) showed that strain shin9-1T belonged to the genus Rhizobium. Strain shin9-1T had the highest level of 16S rRNA gene sequence similarity with respect to Rhizobium daejeonense L61T (97.6 %). The major fatty acid of strain shin9-1T was C18:1 7c. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine and several uncharacterized lipids. The DNA G+C content was 58.3 mol%. The DNA-DNA relatedness of strain shin9-1T with respect to recognized species of the genus Rhizobium was less than 70 %. Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Rhizobium. On the basis of the phylogenetic inference and phenotypic data, strain shin9-1T should be classified as a novel species, for which the name Rhizobium ipomoeae sp. nov. is proposed. The type strain is shin9-1T (=LMG 27163T =KCTC 32148T).
    No preview · Article · Jan 2016 · International Journal of Systematic and Evolutionary Microbiology
  • Shih-Yi Sheu · Wen-Ming Chen · Yi-Ling Chen
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain, designated TTM-1T, was isolated from a water sample taken from the Caohu River in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain TTM-1T were Gram-stain-negative, aerobic, non-motile, showing rod-shaped that were covered by large capsules and formed pink-colored colonies. Growth occurred at 10-37 oC (optimum, 30-37 °C), at pH 6-8 (optimum, pH 6-7) and with 0-2 % NaCl (optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TTM-1T belonged to the genus Mucilaginibacter and was most closely related to Mucilaginibacter defluvii A5T with sequence similarity of 97.3 %. The predominant fatty acids were summed feature 3 (C16:1 7c and/or C16:1 6c; 37.1 %) and iso-C15:0 (30.7 %). The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major isoprenoid quinone was MK-7. The DNA G+C content of the genomic DNA was 45.1 mol%. The DNA-DNA relatedness of strain TTM-1T with respect to recognized species of the genus Mucilaginibacter was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain TTM-1T should be classified as a novel species, for which the name Mucilaginibacter roseus sp. nov. is proposed. The type strain is TTM-1T (=LMG 28454T=KCTC 42273T).
    No preview · Article · Dec 2015 · International Journal of Systematic and Evolutionary Microbiology
  • Zih-Han Chen · Chiu-Chung Young · Wen-Ming Chen · Shih-Yi Sheu
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain, designated KBP-31T, was isolated from a water sample taken from the Banping Lake Wetland Park in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain KBP-31T were Gram-stain-negative, strictly aerobic, motile and light yellow-pigmented rods. Growth occurred at 10-37 oC (optimum, 25 °C), at pH 6-8 (optimum, pH 6) and with 0-1 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KBP-31T belonged to the genus Ideonella and was most closely related to Ideonella dechloratans ATCC 51718T with sequence similarity of 98.2 %. Strain KBP-31T contained summed feature 3 (comprising C16:1 7c and/or C16:1 6c) and C16:0 as the predominant fatty acids. The major hydroxyl fatty acid was C10:0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminophospholipid and two uncharacterized phospholipids. The DNA G+C content of the genomic DNA was 67.9 mol%. The DNA-DNA relatedness of strain KBP-31T with respect to recognized species of the genus Ideonella was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain KBP-31T should be classified as a novel species, for which the name Ideonella paludis sp. nov. is proposed. The type strain is KBP-31T (=BCRC 80524T =KCTC 32238T).
    No preview · Article · Dec 2015 · International Journal of Systematic and Evolutionary Microbiology
  • [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain designated Teta-03T was isolated from a taro field in Taiwan and characterized using the polyphasic taxonomic approach. Cells of strain Teta-03T were aerobic, Gram-stain-negative, rod-shaped, non-motile and formed bright yellow colored colonies. Growth occurred at 10-37 °C (optimum, 20 °C), with 0-1.0 % NaCl (optimum, 0 %) and at pH 3.0-9.0 (optimum, pH 7.0-8.0). The major fatty acids (>10 %) of strain Teta-03T were C18:1 7c, summed feature 3 (C16:17c and/or C16:16c) and C16:0. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine, an uncharacterized glycolipid and an uncharacterized aminolipid. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 65.0 mol%. On the basis of 16S rRNA gene sequence analysis, strain Teta-03T was shown to belong to the genus Novosphingobium and showed the highest similarity to Novosphingobium barchaimii LL02T (96.8 %). Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Novosphingobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Teta-03T represents a novel species in the genus Novosphingobium, for which the name Novosphingobium colocasiae sp. nov. is proposed. The type strain is Teta-03T (=LMG 27385T =KCTC 32255T).
    No preview · Article · Nov 2015 · International Journal of Systematic and Evolutionary Microbiology
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background: Clostridium pasteurianum CH4 was used to produce butanol from glycerol. The performance of butanol fermentation was improved by adding butyrate as the precursor to trigger the metabolic pathway toward butanol production, and by combining this with in situ butanol removal via vacuum membrane distillation (VMD) to avoid the product inhibition arising from a high butanol concentration. Results: Adding 6 g L(-1) butyrate as precursor led to an increase in the butanol yield from 0.24 to 0.34 mol butanol (mol glycerol)(-1). Combining VMD and butyrate addition strategies could further enhance the maximum effective butanol concentration to 29.8 g L(-1), while the yield was also improved to 0.39 mol butanol (mol glycerol)(-1). The butanol concentration in the permeate of VMD was nearly five times higher than that in the feeding solution. Conclusions: The proposed butyrate addition and VMD in situ butanol removal strategies are very effective in enhancing both butanol titer and butanol yield. This would significantly enhance the economic feasibility of fermentative production of butanol. The VMD-based technology not only alleviates the inhibitory effect of butanol, but also markedly increases butanol concentration in the permeate after condensation, thereby making downstream processing easier and more cost-effective.
    Full-text · Article · Oct 2015 · Biotechnology for Biofuels
  • Shih-Yi Sheu · Yi-Ling Chen · Wen-Ming Chen
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain designated TNR-2T was isolated from spring water in Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain TNR-2T were aerobic, Gram-stain-negative, motile by a single polar flagellum, poly--hydroxybutyrate-containing, showing straight and rod-shaped that were covered by large capsules and formed yellow-colored colonies. Growth occurred at 15-37 °C (optimum, 20-30 °C), with 0-1.0 % NaCl (optimum, 0-0.1 %) and at pH 5.0-8.0 (optimum, pH 6.0). According to a phylogenetic tree based on 16S rRNA gene sequence analysis, strain TNR-2T belonged to the genus Sphingomonas and clustered with Sphingomonas alpine S8-3T, with which it shared the highest 16S rRNA gene sequence similarity (95.6 %). The major fatty acids (>10 %) of strain TNR-2T were C18:1 7c, C17:1 6c and C16:0. The DNA G+C content was 62.8 mol%. The major isoprenoid quinone was Q-10. The major polyamine was homospermidine. The polar lipid profile consisted of a mixture of sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylcholine, diphosphatidylglycerol, two uncharacterized glycolipids and an uncharacterized phospholipid. Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Sphingomonas. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain TNR-2T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas fonticola sp. nov. is proposed. The type strain is TNR-2T (=BCRC 80539T =LMG 27384T =KCTC 32258T).
    No preview · Article · Sep 2015 · International Journal of Systematic and Evolutionary Microbiology
  • [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain, designated Npb-02T, was isolated from a freshwater river in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain Npb-02T were Gram-stain-negative, aerobic, poly--hydroxybutyrate-accumulating, rod-shaped and non-motile. Growth occurred at 15-40 °C (optimum, 25-30 °C), at pH 7.0-8.0 (optimum, pH 7.0) and with 0-1 % NaCl (optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-02T belonged to the genus Vogesella and was most closely related to Vogesella perlucida DS-28T with sequence similarity of 98.3 %. Strain Npb-02T contained summed feature 3 (comprising C16:1 7c and/or C16:1 6c) and C16:0 as the major fatty acids. The major respiratory quinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminophospholipid and an uncharacterized phospholipid. The genomic DNA G+C content of strain Npb-02T was 64.1 mol%. The DNA-DNA hybridization values for strain Npb-02T with Vogesella perlucida DS-28T, Vogesella mureinivorans 389T and Vogesella lacus GR13T were less than 25 %. On the basis of the phylogenetic inference and phenotypic data, strain Npb-02T should be classified as a novel species, for which the name Vogesella amnigena sp. nov. is proposed. The type strain is Npb-02T (=BCRC 80887T =LMG 28419T =KCTC 42195T).
    No preview · Article · Jul 2015 · International Journal of Systematic and Evolutionary Microbiology
  • [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain designated TNR-22T was isolated from freshwater river in Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain TNR-22T were faculative anaerobic, Gram-stain-negative, rod-shaped, motile by a single polar flagellum and forming cream colored colonies. Growth occurred at 4-45 °C (optimum, 25-30 °C), with 0-1.0 % NaCl (optimum, 0.5 %) and at pH 7.0-8.0 (optimum, pH 7.0). Strain TNR-22T did not form nodules on a legume plant, Macroptilium atropurpureum. The nifH gene encoding denitrogenase reductase was not detected by PCR. The major fatty acids (>10 %) of strain TNR-22T were C18:1 ω7c and C16:0. The DNA G+C content was 60.3 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, an uncharacterized aminoglycolipid and an uncharacterized phospholipid. Comparative analysis of the 16S rRNA gene sequences showed that strain TNR-22T constituted a distinct branch within the genus Rhizobium, showing the highest level of sequence similarity with respect to Rhizobium rosettiformans W3T (96.3 %). Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Rhizobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain TNR-22T represents a novel species in the genus Rhizobium, for which the name Rhizobium alvei sp. nov. is proposed. The type strain is TNR-22T (=BCRC 80408T =LMG 26895T =KCTC 23919T).
    No preview · Article · Nov 2014 · International Journal of Systematic and Evolutionary Microbiology
  • Source

    Full-text · Dataset · Oct 2014
  • Wen-Ming Chen · Jhen-Ci Chen · Shih-Yi Sheu
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain, designated Jyi-05T, was isolated from a flooded rice field and characterized using a polyphasic taxonomy approach. Cells of strain Jyi-05T were Gram-stain-negative, strictly aerobic, bright yellow-pigmented rods that were motile by gliding. Growth occurred at 10-30 °C (optimum, 30 °C), at pH 6.5-7.5 (optimum, pH 7.0) and with 0-2 % NaCl (optimum, 1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Jyi-05T belonged to the genus Flavobacterium and was most closely related to Flavobacterium flevense DSM 1076T with sequence similarity of 97.6 %. Strain Jyi-05T contained iso-C15:0 (16.6 %), anteiso-C15:0 (10.8 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c; 10.3 %), iso-C17:0 3-OH (9.2 %), C16:0 (7.3 %) and iso-C15:0 3-OH (7.2 %) as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. Genomic DNA G+C content of strain Jyi-05T was 34.2 mol%. The DNA-DNA relatedness of strain Jyi-05T with respect to recognized species of the genus Flavobacterium was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain Jyi-05T should be classified as a novel species, for which the name Flavobacterium oryzae sp. nov. is presented. The type strain is Jyi-05T (=BCRC 80522T =LMG 27292T =KCTC 32240T). Emended descriptions of Flavobacterium flevense, Flavobacterium yonginense and Flavobacterium myungsuense are also proposed.
    No preview · Article · Aug 2014 · International Journal of Systematic and Evolutionary Microbiology
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The South African invasive legume Dipogon lignosus (Phaseoleae) produces nodules with both determinate and indeterminate characteristics in New Zealand (NZ) soils. Ten bacterial isolates produced functional nodules on D. lignosus. The 16S ribosomal RNA (rRNA) gene sequences identified one isolate as Bradyrhizobium sp., one isolate as Rhizobium sp. and eight isolates as Burkholderia sp. The Bradyrhizobium sp. and Rhizobium sp. 16S rRNA sequences were identical to those of strains previously isolated from crop plants and may have originated from inocula used on crops. Both 16S rRNA and DNA recombinase A (recA) gene sequences placed the eight Burkholderia isolates separate from previously described Burkholderia rhizobial species. However, the isolates showed a very close relationship to Burkholderia rhizobial strains isolated from South African plants with respect to their nitrogenase iron protein (nifH), N-acyltransferase nodulation protein A (nodA) and N-acetylglucosaminyl transferase nodulation protein C (nodC) gene sequences. Gene sequences and enterobacterial repetitive intergenic consensus (ERIC) PCR and repetitive element palindromic PCR (rep-PCR) banding patterns indicated that the eight Burkholderia isolates separated into five clones of one strain and three of another. One strain was tested and shown to produce functional nodules on a range of South African plants previously reported to be nodulated by Burkholderia tuberum STM678(T) which was isolated from the Cape Region. Thus, evidence is strong that the Burkholderia strains isolated here originated in South Africa and were somehow transported with the plants from their native habitat to NZ. It is possible that the strains are of a new species capable of nodulating legumes.
    Full-text · Article · May 2014 · Microbial Ecology
  • Shih-Yi Sheu · Zih-Han Chen · Chiu-Chung Young · Wen-Ming Chen
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain designated KBP-21T was isolated from a water sample taken from the Banping Lake Wetland Park in Taiwan and characterized using the polyphasic taxonomic approach. Cells of strain KBP-21T were Gram-staining-negative, facultatively anaerobic, poly-β-hydroxybutyrate-accumulating, motile rods and forming yellow-colored colonies. Growth occurred at 15-40 oC (optimum, 30 °C), at pH 5.0-8.0 (optimum, pH 8.0) and with 0-2 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KBP-21T belonged to the genus Paludibacterium within the family Neisseriaceae of the class Betaproteobacteria and its most closely related neighbour was Paludibacterium yongneupense 5YN8-15T with sequence similarity of 96.4 %. Strain KBP-21T contained summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C16:0 and C18:1 ω7c as the predominant fatty acids. The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 62.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one uncharacterized aminophospholipid and several uncharacterized phospholipids. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain KBP-21T represents a novel species in the genus Paludibacterium, for which the name Paludibacterium paludis sp. nov. is proposed. The type strain is KBP-21T (=BCRC 80514T =LMG 27230T =KCTC 32182T).
    No preview · Article · May 2014 · International Journal of Systematic and Evolutionary Microbiology
  • Source
    Shih-Yi Sheu · Jhen-Ci Chen · Chiu-Chung Young · Wen-Ming Chen
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterium strain, designated Npb-03T was isolated from freshwater river in Taiwan and was characterized using the polyphasic taxonomy approach. The cells were Gram-negative, straight rod-shaped, non-motile, non-spore forming and faculatively anaerobic. Growth occurred at 10-37 °C (optimum, 30-35 °C), at pH 6.0-8.0 (optimum, pH 6.0-7.0) and with 0-1.0 % NaCl (optimum, 0 %). The predominant fatty acids were summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) and C16:0. The major isoprenoid quinone was Q-8 and the DNA G+C content was 64.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and three uncharacterized phospholipids. The major polyamines were putrescine, 2-hydroxyputrescine, cadaverine and spermidine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-03T forms a distinct lineage with respect to closely related genera within the family Neisseriaceae of the class Betaproteobacteria, most closely related to Aquaspirillum, Laribacter, Leeia and Microvirgula, and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 93 %. On the basis of the genotypic and phenotypic data, strain Npb-03T represents a novel genus and species of the family Neisseriaceae, for which the name Rivicola pingtungensis gen. nov., sp. nov. is proposed. The type strain is Npb-03T (=BCRC 80376T =LMG 26668T =KCTC 23712T).
    Preview · Article · Mar 2014 · International Journal of Systematic and Evolutionary Microbiology
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A continuous fixed-bed biosorption process was established for cadmium (Cd) removal by Scenedesmus obliquus CNW-N (isolated from southern Taiwan) cells immobilized onto loofa sponge. This immobilized-cell biosorption process allows better recovery and reusability of the microalgal biomass. The growth of microalgae on the matrix support with appropriate nutrient supplementation could enhance the overall metal removal activity. Major operating parameters (e.g., feeding flow rate, cycle number of medium replacement, and particle diameter of the sponge) were studied for treatability evaluation. The most promising cell growth on the sponge support was obtained at a flow rate of 0.284 bed volume (BV)/min, sponge particle diameter of 1cm, and with one cycle of medium replacement. The performance of fixed-bed biosorption (adsorption capacity of 38.4mg, breakthrough time at 15.5h) was achieved at a flow rate of 5ml/min with an influent concentration of 7.5mgCd/l.
    Full-text · Article · Feb 2014 · Bioresource Technology
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated strain KIS83-12T, was isolated from soil of Gaui island in Taean region, South Korea. The strain grew at 15-33 °C (optimum, 28 °C), at pH 5.0-8.0 (optimum, pH 7.0). Growth did not occur in the presence of NaCl. The strain was catalase-negative and oxidase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that KIS83-12T was related most closely to Solimonas soli DCY12T (96.9%), Solimonas variicoloris MN 28T (96.5%), Solimonas flava CW-KD 4T (96.5%) and Solimonas aquatica NAA16T (96.0%), and formed a robust phyletic lineage with members of the genus Solimonas. Main isoprenoid quinone was Q-8. Major polar lipids included phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. A large and moderate amounts of fatty acids (>5.0%) were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, C16:1 ω5c, summed feature 2 (iso-C16:1 I and/or C14:0 3-OH) and C12:0. The DNA G+C content was 67.9 mol%. On the basis of taxonomic data obtained in this study, KIS83-12T represents a novel species of the genus Solimonas, for which the name Solimonas terrae sp. nov. is proposed, with KIS83-12T ( = KACC 16967T = DSM 27281T) as the type strain.
    Preview · Article · Jan 2014 · International Journal of Systematic and Evolutionary Microbiology
  • Source
    Shih-Yi Sheu · Yang-Shun Lin · Jhen-Ci Chen · Wen-Ming Chen
    [Show abstract] [Hide abstract]
    ABSTRACT: A bacterial strain designated CMJ-9T was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain CMJ-9T were strictly aerobic, Gram-negative, motile by a single polar flagellum, poly-β-hydroxybutyrate-containing and forming light yellow-pigmented colonies. Growth occurred at 10-37 °C (optimum, 20-30 °C), with 0-0.8 % NaCl (optimum, 0-0.1 %) and at pH 6.0-9.0 (optimum, pH 6.0-7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-9T belonged to the genus Undibacterium and its closest neighbour was Undibacterium seohonense SHS5-24T with sequence similarities of 96.7 %. The predominant cellular fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16:0. The major cellular hydroxy fatty acid was C10:0 3-OH. The polar lipid profile consisted of the predominant lipids phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 47.7 mol%. On the basis of the phylogenetic and phenotypic data, strain CMJ-9T should be classified as representing a novel species, for which the name Undibacterium macrobrachii sp. nov. is proposed. The type strain is CMJ-9T (=BCRC 80406T =LMG 26891T =KCTC 23916T).
    Preview · Article · Dec 2013 · International Journal of Systematic and Evolutionary Microbiology
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Variations in the patterns of diversity of symbionts have been described worldwide on Mimosa pudica, a pan-tropical invasive species that interacts with both α and β-rhizobia. In this study, we investigated if symbiont competitiveness can explain these variations and the apparent prevalence of β- over α-rhizobia. We developed an indirect method to measure the proportion of nodulation against a GFP reference strain and tested its reproducibility and efficiency. We estimated the competitiveness of 54 strains belonging to four species of β-rhizobia and four of α-rhizobia, and the influence of the host genotype on their competitiveness. Our results were compared with biogeographical patterns of symbionts and host varieties. We found: (i) a strong strain effect on competitiveness largely explained by the rhizobial species, with Burkholderia phymatum being the most competitive species, followed by B. tuberum, whereas all other species shared similar and reduced levels of competitiveness; (ii) plant genotype can increase the competitiveness of Cupriavidus taiwanensis. The latter data support the likelihood of the strong adaptation of C. taiwanensis with the M. pudica var. unijuga and help explain its prevalence as a symbiont of this variety over Burkholderia species in some environments, most notably in Taiwan.
    Full-text · Article · Sep 2013 · Environmental Microbiology

Publication Stats

4k Citations
510.89 Total Impact Points

Institutions

  • 2004-2016
    • National Kaohsiung Marine University
      Kao-hsiung-shih, Kaohsiung, Taiwan
  • 2009-2014
    • National Chung Hsing University
      • Department of Soil and Environmental Sciences
      臺中市, Taiwan, Taiwan
  • 2008
    • National Cheng Kung University
      • Department of Chemical Engineering
      臺南市, Taiwan, Taiwan