E I Adamskaya

Russian Academy of Medical Sciences, Moskva, Moscow, Russia

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Publications (20)7.4 Total impact

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    ABSTRACT: The role of endogenous luteinizing hormone-releasing hormone (LHRH) in the development of concanavalin A (ConA)-induced proliferative responses was studied in rat fetuses. Preliminary treatment of fetuses in utero with either the LHRH receptor antagonist or anti-LHRH antibodies resulted in the suppression of ConA-induced proliferative responses of thymocytes. LHRH and LHRH-immunopositive cells, morphologically similar to thymocytes, were detected in intact fetal thymus. A significant content of LHRH was also found in the peripheral blood of fetuses. The LHRH content in thymus and plasma was similar in males and females. Surgical ablation of the hypothalamus resulted in 2-fold decreases in thymus and plasma levels of LHRH in 21-day-old fetuses compared to sham-operated fetuses. It was concluded that LHRH regulates mitogen-induced proliferative responses of thymocytes during prenatal ontogenesis in the rat. The main source of plasma LHRH at that period is the hypothalamus. Moreover, LHRH is synthesized in the fetal thymus. Thus, LHRH is suggested to have not only a central effect but also to be involved in autocrine or paracrine regulation of proliferative immune responses.
    No preview · Article · Feb 2005 · NeuroImmunoModulation
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    ABSTRACT: This study used a pharmacological approach to evaluate the consequences of the metabolic perturbations of neurotransmitters on brain development. Pregnant rats received p-chlorophenylalanine (pCPA), an inhibitor of serotonin (5-hydroxytryptamine, 5-HT) synthesis, or saline (control) from the 11th day of gestation once or daily up to the 15th, 17th and 20th day, followed by processing of the forebrain and/or nasal cranium of foetal males and females for high-performance liquid chromatography of monoamines, radioimmunoassay of gonadotropin-releasing hormone (GnRH) and quantitative and semiquantitative immunocytochemistry for GnRH. The pCPA treatment resulted in a 50-70% depletion of 5-HT in the nasal crania and forebrains at any studied age. Radioimmunoassay showed no change in GnRH content in 5-HT deficient foetuses at E16 compared to controls, being higher in both cases in the rostral forebrain than in the hypothalamus. In controls at E21, the GnRH content in the hypothalamus exceeded that in the rostral forebrain, whereas in the 5-HT deficient group the opposite was found. These data suggest that 5-HT provided a stimulating effect on GnRH neurone migration, and this was confirmed by quantification of GnRH-immunoreactive neurones in the forebrain along the trajectory of their migration. At E18 and E21, the fractions of GnRH neurones in the rostral part of the trajectory in pCPA-treated foetuses were greater than those in control foetuses but the opposite was true for the caudal part of the trajectory. Moreover, 5-HT appeared to control the proliferation of the precursor cells of GnRH neurones and their differentiation, as derived from the observations of the increased number of GnRH neurones in the forebrain of foetuses of both sexes, as well as the region-specific decreased neuronal size and content of GnRH in 5-HT-deficient females. Thus, 5-HT appears to contribute to the regulation of the origin, differentiation and migration of GnRH neurones.
    Full-text · Article · Jul 2003 · Journal of Neuroendocrinology

  • No preview · Article · Mar 2003 · Doklady Biological Sciences
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    ABSTRACT: Role of sex hormones in the development of pituitary adenomas was investigated by analyzing the content of nuclear estradiol and testosterone receptors in different tumors of the anterior pituitary: prolactinomas, meningiomas, growth hormone-producing adenomas, astrocytomas, neurinomas, and ependymomas. The concentration of nuclear estrogen and androgen receptors in prolactin-secreting pituitary adenomas was much higher than in growth hormone-producing adenomas and other pituitary tumors.
    No preview · Article · May 2001 · Bulletin of Experimental Biology and Medicine
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    ABSTRACT: The effect of endogenous luteinizing hormone-releasing hormone (LHRH) on the proliferation induced by concanavalin A (Con A) in rat fetal thymocytes was studied. A selective antagonist (2 microg per fetus) or antibodies to LHRH (20 microl per fetus) were injected in utero into 20-day-old rat fetuses, and this resulted in a two- or fivefold decrease in the Con A-induced proliferation of thymocytes, respectively. In combined culture of the antagonist (10-5-10-6 M) with fetal thymocytes, the proliferative response was not decreased. The concentration of LHRH was determined by radioimmunoassay in tissues of immunocompetent organs and in blood serum of 18- and 21-day-old fetuses, and the hormone was found in the hypothalamus, thymus, and peripheral blood. The initially low level of LHRH in the thymus increased by 65 and 40%, respectively, on the first day after birth and became similar to the level in the hypothalamus. In the fetal blood serum, the LHRH level was significantly higher than in the thymus and hypothalamus of fetuses of the same age. The hormone concentration was greatest in the 18-day-old fetuses, and it decreased twofold by the 21st day. The findings indicate that LHRH is involved in regulation of T-cell immunity even during prenatal ontogenesis.
    No preview · Article · Nov 2000 · Biochemistry (Moscow)
  • V N Babichev · E I Adamskaya · TA Kuznetsova · I V Shishkina
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    ABSTRACT: 1. Development of experimental diabetes in male rats is associated with reductions in the blood T concentration and in the number of nuclear androgen receptors in the adenohydraphysis. Blood LH and FSH levels did not change. 2. The LH-RH sensitivity of the hypophysis in STZ-treated male rats decreased sharply on incubation for more than 1h. 3. Administration of STZ to female rats led to disruption of the estral cycle, accompanied by a reductions in the cyclic secretion of gonadotropins and sex hormones. 4. The development of diabetes in cycling female rats led to a reduction in the concentration of nuclear E2 receptors in the PR and hypophysis, and the number of T-binding sites decreased only in the hypophysis. 5. The gonadotropin wave stimulated by sex steroids in ovariectomized female rats with diabetes was significantly reduced, due to changes in the activity of the LH-RH-producing system.
    No preview · Article · Jan 1998 · Neuroscience and Behavioral Physiology
  • V N Babichev · E I Adamskaya · T A Peryshkova
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    ABSTRACT: It is known that the disturbance in the functional activity of the reproductive system of the organism in diabetic laboratory animals is associated not only with destructive changes in the gonads, but with dysfunction of the hypothalamo-hypophyseal complex as well [11, 12]. It has been demonstrated that the decrease in the basal secretion of gonadotropins and sex hormones and the absence of its cyclical changes in female rats with experimental diabetes may be determined by disturbances in the central hypothalamic regulation of the reproductive system [6, 14]. The possibility of changes in the secretion of gonadotropins that are associated with a disturbance in the sensitivity of gonadotrops of the hypophysis to the action of luliberin (LH-RH) has not been excluded. Also, it is known from the literature that these disturbances are manifested to different degrees in male and female individuals [3]. The question as to the degree to which the disturbances arising in diabetes depend on the level of sex steroids and insulin in the organism remains controversial [4, 19]. Considering the fact that insulin may participate directly in the regulation of the function of the gonadotrops [2], we investigated under in vitro conditions its influence on the sensitivity of the hypophysis to LH-RH in a model of ovariectomized female rats with streptozotocin-induced diabetes which received estradiol as compensatory hormone therapy.
    No preview · Article · Jan 1995 · Neuroscience and Behavioral Physiology
  • V N Babichev · E I Adamskaya · T A Peryshkova
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    ABSTRACT: Investigations to assess the state of the reproductive system in female rats in experimental streptozotocin (STZ)-induced diabetes have been a logical continuation of studies we have carried out previously [4, 5] to analyze the interrelationship of disturbances of the reproductive system and sexual behavior in male rats in diabetes. It is known that by contrast with males, cyclical changes in the activity of the reproductive system are characteristic for females. Consequently, it is not excluded that the differences observed in the norm in the regulation of the gonadotropic function of the hypophysis in male and female rats may explain the fact that males are in the main more sensitive to the hyperglycemic action of STZ than females [13]. In addition, some data point to nonidentical changes in the organism of males and females in the presence of an insulin deficit [10]. Bestetti et al. [7] have concluded, on the basis of the results of morphological and physiological investigations, that changes in the hypophysis are less clearly manifested in the females than the males. The interpretation of the results obtained by various investigators when studying the reproductive system in diabetic females has been complicated by the presence of cyclical changes. The disturbances in cyclicity in the severe forms of diabetes may be caused by the numerous disturbances of the hypothalamohypophyseogonadal axis, which includes both CNS structures and the ovarian level. A comprehensive investigation was carried out in this study of the of the functional activity of the hypothalamo-hypophyseogonadal system in female rats with streptozotocin-induced diabetes. The concentration of nuclear receptors of the sex hormones in the adenohypophysis and regions of the hypothalamus which participate in the regulation of the secretion of the gonadotropins through the feedback mechanism was determined for this purpose. In addition, the sensitivity of the hypophysis to luliberin (LH-RH) in ovariectomized female rats with compensatory administration of estradiol (E2) and progesterone was determined to exclude a possible disturbance in the secretion of steroids at the ovarian level in diabetes.
    No preview · Article · Jan 1995 · Neuroscience and Behavioral Physiology
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    ABSTRACT: Luteinizing hormone-releasing hormone drops in the hypothalamus of male but not female rat fetuses in serotonin (5-HT) deficiency. This drop is coincident with an increase of the luteinizing hormone (LH) level in blood plasma of males. An influence of testosterone on LH-RH or LH secretion should be ruled out, because the level of testosterone is not changed in the testes and blood plasma.
    No preview · Article · May 1994 · Bulletin of Experimental Biology and Medicine
  • V N Babichev · T A Peryshkova · E I Adamskaya
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    ABSTRACT: 1. The level of LH and FSH in the blood of male rats did not change during the development of experimental diabetes; the concentration of T decreased. 2. The sensitivity of the hypophysis to LH-RH in rats that have received STZ declined sharply when it was incubated for more than 1 h. 3. A decrease in the concentration of the nuclear T receptors in the adenohypophysis was observed during the development of experimental diabetes. 4. The alterations in the concentration of hormones in the blood and in the level of androgen receptors in the hypophysis of rats receiving STZ were restored through insulin replacement therapy.
    No preview · Article · Mar 1994 · Neuroscience and Behavioral Physiology
  • T V El'tseva · E I Adamskaya · T A Peryshkova · V N Babichev
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    ABSTRACT: 1. A decrease in the level of activity of both motivational and copulatory components of sexual behavior was observed during the development of experimental diabetes following the administration of streptozotocin to rat males. 2. The concentration of LHRH in diabetic rats decreased in the ME [sic], and similar changes were recorded in both the PA and the MBH when it was determined in the synaptosomal fraction of the hypothalamus. 3. The concentration of nuclear E2 receptors was increased in the APA at the level of the hypothalamus, whereas the number of testosterone-binding sites did not differ from the values of the control group. 4. The level of LH and FSH in the blood of the rats did not change during the development of diabetes, the concentration of PRL and T decreased, and that of E2 increased.
    No preview · Article · Nov 1993 · Neuroscience and Behavioral Physiology
  • V N Babichev · E I Adamskaya · Ozol' LYu · T V El'tseva
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    ABSTRACT: 1. The noradrenergic and serotoninergic innervation of the OB and the noradrenergic innervation of the POA participate in the interaction of these structures of the limbic system through the LHRH-synthesizing neurons. 2. The secretion of LHRH from the nerve terminals of the OB and the POA is under the inhibitory influence of serotonin, and the secretion of the peptide from the nerve terminals of the MBH is activated by norepinephrine. 3. LHRH exerts a varied influence on sexual behavior of the male rat when it is liberated in different structures of the limbic system: at the level of the OB the peptide influences the length of the post-ejaculatory refractory period, and at the level of the POA, it affects the latent interval of ejaculation.
    No preview · Article · Jul 1991 · Neuroscience and Behavioral Physiology
  • V N Babichev · T A Peryshkova · E I Adamskaya
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    ABSTRACT: 1. The administration of a blocker of a-adrenoreceptors does not influence the blood content of LH and FSH in the early hours of the E stage. 2. The administration of a blocker of DA (haloperidol) decreased, while a stimulator of the action (parlodel) increased the concentration of FSH in the blood at the 1 and 3 h stages of E without changes in the blood of LH. 3. An interconnection between the decrease in the secretion of FSH and the level of nuclear E2 receptors in the adenohypophysis in the early hours of the E stage.
    No preview · Article · May 1991 · Neuroscience and Behavioral Physiology
  • T V El'tseva · E I Adamskaya · Ozol' LYu · V N Babichev
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    ABSTRACT: We have studied the level of LH-RH in the synaptosomal fraction of the olfactory bulbs, preoptic area, and mediobasal hypothalamus, and the blood level of HL in adult male rats with sexual activity after noradrenergic denervation of the preoptic area using 6-oxydopamine. A neurotoxic effect of a 0.1% solution of ascorbic acid, preventing 6-oxydopamine disintegration was observed. Both sexual activity and level of LH-RH in synaptosomes of the preoptic area were reduced in male rats with noradrenergic denervation of the preoptic area. The maximum blood level of LH was observed during exposure to a recipient female rat, the LH-RH concentration in the synaptosomal fraction of the olfactory bulbs being raised, and that in the mediobasal hypothalamus being decreased. The results showed that LH-RH synthesizing cerebral neurons with terminals in the olfactory bulbs, preoptic area, and mediobasal hypothalamus are involved in the regulation of sexual behavior and the interaction of LH-RH-containing terminals with noradrenergic nerve endings at the level of the preoptic area.
    No preview · Article · May 1989 · Neuroscience and Behavioral Physiology
  • V N Babichev · N G Bukiya · E I Adamskaya
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    ABSTRACT: 1. Administration of phentolamine (α-adrenoblocker) decreases the LH level in the blood of male rats. Administration against this background of mezatone (α-adrenostimulator) or apomorphine (DA stimulator) reverses the effect of phentolamine. 2. On administration into the PA or ARC, NA stimulates the release of gonadotropins from the adenohypophysis, by acting on the neurons of the hypothalamus producing LH-RH. The steroiddependent effect of NA is expressed only at the level of the rostral hypothalamus. 3. Administration of DA into the PA and ARC changes the concentration of LH-RH in the rostral and mediobasal sections of the hypothalamus, and increases the level of gonadotropins in the blood of intact males. However, the DA effect was steroid-dependent only at the level of the mediobasal hypothalamus.
    No preview · Article · Nov 1988 · Neuroscience and Behavioral Physiology
  • E I Adamskaya · V N Babichev
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    ABSTRACT: 1. The amplitude of the circadian fluctuations of the level of NE, DA, and 5-HT in the hypothalamus of female rats depends on the phase of the estrous cycle: the most pronounced deviations from the circadian rhythm were observed in the preovulatory period in the anterior and mediobasal divisions of the hypothalamus. 2. All three investigated monoamines participate in the regulation of cyclic LH secretion. The stimulatory effects of NE and the inhibitory effects of 5-HT at the level of the cyclic center of the hypothalamus in the control of the gonadotropic function of the pituitary are manifested in the presence of an increased content of estrogens in the circulating blood. At the level of the tonic center of the hypothalamus, the stimulating effect of DA is also estrogen-dependent.
    No preview · Article · Mar 1986 · Neuroscience and Behavioral Physiology
  • V N Babichev · E I Adamskaya
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    ABSTRACT: Injection of serotonin into the mediobasal hypothalamus inhibits LH secretion in phase P. An analogous effect with serotonin injection into the PO region is seen only in the initial period of this stage. Injection of serotonin into the PO region of OE rats does not alter blood concentration of LH, while injection into the mediobasal region results in a decrease in blood LH. Pretreatment of the OE animals with estradiol leads to a recovery of the inhibitory effects of serotonin injected into the rostral hypothalamus and blocks its effects on the release of LH when it is injected into the mediobasal hypothalamus.
    No preview · Article · Mar 1983 · Neuroscience and Behavioral Physiology
  • E I Adamskaya · V N Babichev
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    ABSTRACT: 1. DA is involved in the control of the ovulatory release of LH and acts in the ARC-ME. 2. The effects of DA on the gonadotropic function are determined by the levels of circulating estrogens in the blood. The maximum response is recorded in the second half of the diestrus-2 stage.
    No preview · Article · Sep 1982 · Neuroscience and Behavioral Physiology
  • E. I. Adamskaya · V. N. Babichev

    No preview · Article · Jun 1981 · Bulletin of Experimental Biology and Medicine
  • V N Babichev · E I Adamskaya
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    ABSTRACT: 1. The monoamine content in the various parts of the hypothalamus changes during the estrous cycle. 2. In the course of the estrous cycle the DA concentration in the arcuate region reaches a maximum at 6 p.m. of stage D2 and falls to a minimum at 10 a.m. of stage P. 3. The NA level in the preoptic region is raised at 6 p.m. of stage D2 and lowered at 10 a.m. of stage P.
    No preview · Article · Jul 1976 · Neuroscience and Behavioral Physiology