Publications (80)

  • Yasemin G Kurt · Bulent Kurt · Ibrahim Aydin · [...] · Fevzi N Aydin
    [Show abstract] [Hide abstract] ABSTRACT: Mitochondrial respiratory chains consist of approximately 100 structural proteins. Thirteen of these structural proteins are encoded by mitochondrial DNA (mtDNA), and the others by nuclear DNA (nDNA). Mutation in any of the mitochondrial structural-protein related genes, regardless of whether they are in the nDNA or mtDNA, might cause mitochondrial disorders. In the recent past, new nuclear genes required for assembly, maintenance, and translation of respiratory chain proteins have been found. Mutation in these genes might also cause mitochondrial disorders (MD). NFU1 gene is one of such genes and has a role in the assembly of iron-sulfur cluster (ISC). ISCs are included in a variety of metalloproteins, such as the ferredoxins, as well as in enzymatic reactions and have been first identified in the oxidation-reduction reactions of mitochondrial electron transport. It is important to be aware of NFU1 gene mutations that may cause severe mitochondrial respiratory chain defects, mitochondrial encephalomyopathies and death, early in life.
    Article · Jul 2016 · Neurology India
  • Article · Jan 2016
  • [Show abstract] [Hide abstract] ABSTRACT: Glycogen Storage Disease type IV (GSD IV) is a rare autosomal recessive disorder caused by deficiency of the glycogen branching enzyme (GBE). The diagnostic hallmark of the disease is the accumulation of a poorly branched form of glycogen known as polyglucosan (PG). The disease is clinically heterogeneous, with variable tissue involvement and age at onset. Complete loss of enzyme activity is lethal in utero or in infancy and affects primarily muscle and liver. However, residual enzyme activity as low as 5-20% leads to juvenile or adult onset of a disorder that primarily affects the central and peripheral nervous system and muscles and in the latter is termed adult polyglucosan body disease (APBD). Here we describe a mouse model of GSD IV that reflects this spectrum of disease. Homologous recombination was used to knock-in the most common GBE1 mutation p.Y329S c.986A>C found in APBD patients of Ashkenzi Jewish decent. Mice homozygous for this allele (Gbe1(ys/ys)) exhibit a phenotype similar to APBD, with wide spread accumulation of polyglucosan. Adult mice exhibit progressive neuromuscular dysfunction and die prematurely. While the onset of symptoms is limited to adult mice, polyglucosan accumulates in tissues of newborn mice but is initially absent from the cerebral cortex and heart muscle. Thus, polyglucosan is well tolerated in most tissues but the eventual accumulation in neurons and their axons causes neuropathy that leads to hind limb spasticity and premature death. This mouse model mimics the pathology and pathophysiologic features of human adult-onset branching enzyme deficiency.
    Article · Sep 2015 · Human Molecular Genetics
  • E Emer · O Yildiz · M Seyrek · [...] · A Sayal
    [Show abstract] [Hide abstract] ABSTRACT: The aim of this study is to assess cardiotoxic effect of testosterone (TES) and dehydroepiandrosterone (DHEA) in Sprague Dawley rats. We compared the impact of subacute (14 days) and subchronic (90 days) administration of suprapharmacologic doses of TES and DHEA on body weight, locomotor activity, muscle strength, echocardiographic parameters, heart histopathology, and oxidative stress markers with the control group. Testosterone (10, 30, and 100 mg/100 g body weight) and DHEA (10 mg/100 g body weight) administration decreased the body weights and locomotor activity (p < 0.05), and the combination of both increased muscle strength (p < 0.05) in rats. In our histopathological evaluation, misshapen cell nuclei, disorganized myocardial fibers, and leukocytic infiltrates were observed in high-dose TES (100 mg/100 g)-treated rats, especially on day 14. On day 90, mild changes such as misshapen cell nuclei, disorganized myocardial fibers, and leukocytic infiltrates were observed in TES and DHEA-treated groups. According to our echocardiographic study on day 14 and day 90, TES, especially at high doses, induced increase in left ventricular posterior wall diameter and ejection fraction (p < 0.05). In this study, blood oxidative stress marker malondialdehyde was increased slightly but not significantly in TES and DHEA groups. On the other hand, antioxidant enzymes such as SOD and glutathione peroxidase (GSH-Px) levels were slightly but not significantly increased in TES and DHEA groups. These data demonstrate that the potential risk to cardiac health due to exogenous androgen use may be related to oxidative stress in rats. © The Author(s) 2015.
    Article · Jul 2015 · Human & Experimental Toxicology
  • Cengizhan Acikel · YaseminGulcan Kurt · Bulent Kurt · [...] · Safak Ekinci
    [Show abstract] [Hide abstract] ABSTRACT: Muscle biopsy samples must be frozen with liquid nitrogen immediately after excision and maintained at -80°C until analysis. Because of this requirement for tissue processing, patients with neuromuscular diseases often have to travel to centers with on-site muscle pathology laboratories for muscle biopsy sample excision to ensure that samples are properly preserved. Here, we developed a preservative solution and examined its protectiveness on striated muscle tissues for a minimum of the length of time that would be required to reach a specific muscle pathology laboratory. A preservative solution called Kurt-Ozcan (KO) solution was prepared. Eight healthy Sprague-Dawley rats were sacrificed; striated muscle tissue samples were collected and divided into six different groups. Muscle tissue samples were separated into groups for morphological, enzyme histochemical, molecular, and biochemical analysis. Chi-square and Kruskal Wallis tests. Samples kept in the KO and University of Wisconsin (UW) solutions exhibited very good morphological scores at 3, 6, and 18 hours, but artificial changes were observed at 24 hours. Similar findings were observed for the evaluated enzyme activities. There were no differences between the control group and the samples kept in the KO or UW solution at 3, 6, and 18 hours for morphological, enzyme histochemical, and biochemical features. The messenger ribonucleic acid (mRNA) of β-actin gene was protected up to 6 hours in the KO and UW solutions. The KO solution protects the morphological, enzyme histochemical, and biochemical features of striated muscle tissue of healthy rats for 18 hours and preserves the mRNA for 6 hours.
    Article · Apr 2015 · Annals of Indian Academy of Neurology
  • Yasemin KURT · Bulent KURT · Omer Ozcan
    [Show abstract] [Hide abstract] ABSTRACT: Muscle biopsy samples must be frozen in liquid nitrogen immediately after taken from patient. This requirement is a major obstacle to the transfer of muscle biopsies. Therefore, patients with neuromuscular disease often have to travel to centers with on-site muscle pathology laboratories for muscle biopsy. We developed a preservative solution named Kurt-Ozcan solution to transfer the patients' biopsy material. We have shown that it protects the morphological features of muscle tissue for 18 hours at +4oC. This study was done to test whether the protective effect of it on muscle tissue continues 6 months after it produced. One Sprague- Dawley rat was sacrificed; striated muscle tissue samples were collected and kept in Kurt-Ozcan Solution. Groups were compared morphologically with samples frozen in liquid nitrogen. Modified Gomori-Trichrome and Hematoxylen-eosine stains were used for morphological evaluation. Evaluation was conducted semiquantitatively by scoring edema, organization and artificial changes. The mean scores of the groups kept in Kurt-Ozcan solution for 3, 6, and 18 hours were 0.50, 0.66 and 0.82 respectively and there were no differences with the control group scores. Kurt-Ozcan solution kept at +4oC for 6 months is seen to continue to preserve the morphological characteristics of muscle tissue up to 18 hours.
    Article · Jan 2015 · Gulhane Medical Journal
  • Armagan Gunal · Bulent Kurt · Muharrem Oztas · [...] · Yildirim Karslioglu
    [Show abstract] [Hide abstract] ABSTRACT: In colorectal resections, to use the number of metastatic lymph nodes as a reliable tumor staging criterion, the total number of dissected lymph nodes must be above a certain threshold. However, it is difficult to reach required number after neoadjuvant treatment. In this study, we especially evaluated the factors which may affect the number of dissected lymph nodes from rectum resections received neoadjuvant therapy. One hundred two rectum resections were reviewed. The relation with the number of dissected lymph nodes and some criteria of patient and tumor were evaluated. The number of dissected lymph nodes was greater in high grade tumors from younger patients showing mucinous component, perineural invasion, positive radial surgical margin, and with metastatic lymph node. Inadequate tumor regression, extramural tumor depth, and being resected in recent years were also correlated with increased number of dissected lymph nodes. We noticed that the diameter of the largest lymph node has an impact on the total number of lymph nodes. Among these criteria, the patient's age, tumor depth and the diameter of the largest dissected lymph node were most statistically significant. We evaluated the rectum resections after neoadjuvant therapy as a unique model and discussed the factors should be considered while dissecting the lymph nodes.
    Article · Jan 2015 · Gulhane Medical Journal
  • Article · Oct 2014 · Leukemia Research
  • [Show abstract] [Hide abstract] ABSTRACT: To determine a paradigm that will be helpful for urologists to manage fibrous pseudotumors, which are a very rare condition of the testis. We retrospectively evaluated the patients with fibrous pseudotumors in our uropathological database from 1995 to 2013. Patients who had tumor markers and ultrosonography (USG) screening before surgery and a final pathology report of a fibrous pseudotumor were included in the study. In total, 838 patients with a testis mass were evaluated. Only 6 of these patients met the inclusion criteria. The mean age was 34 years (min: 20, max: 72). Serum tumor markers were in the normal range for all patients. The scrotal tumors were not clearly related to the testis parenchyma, and the radiologists could not definitively determine the nature of the masses (benign or malignant). A concomitant hydrocele was detected in 2 patients. After inguinal exploration, radical orchiectomy was performed in one patient with an ipsilateral atrophic testis, and biopsies were taken from the lesions for frozen section analysis in the other 5 patients. Pathologists reported benign tumors for all of these patients based on the frozen sections, and testicular sparing surgery was then performed in these 5 patients. If scrotal tumors are detected by ultrasonography in patients with normal tumor markers, and the tumor cannot be clear distinguished from the testis, these patients might have a fibrous pseudotumor, and organ-sparing surgery can be performed on these patients.
    Article · Sep 2014 · Turk Uroloji Dergisi
  • Nuri Yigit · Yildirim Karslioglu · Bulent Kurt
    [Show abstract] [Hide abstract] ABSTRACT: A radical prostatectomy affects the prostate, bilateral seminal vesicles (SV), and the distal parts of the bilateral vasa deferentia (VD). SV invasion (SVI) is associated with an increased risk of lymph node metastasis and recurrence. However, the significance of VD invasion (VDI), either with or without the involvement of their surgical margins, has not been fully appreciated. We think VDI might have an independent prognostic significance, as does SVI, and should be incorporated into the pathology guidelines and the staging systems of prostatic adenocarcinoma. Our case illustrates this.
    Article · Jul 2014
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    [Show abstract] [Hide abstract] ABSTRACT: Functional nerve regeneration after reconstructive nerve surgery remains unsatisfying. In this study, vascular endothelial growth factor (VEGF) gene therapy combined with a hyaluronic acid (HA)-enriched microenvironment in nerve regeneration was investigated. Sciatic nerve was transected, and end-to-end neurorrhaphy was performed on 32 male Sprague-Dawley rats, which were randomly divided into four groups (n = 8 per group): nerve coaptation without treatment (group I); nerve coaptation covered with HA film sheath (group II); nerve coaptation with intramuscular VEGF gene in plasmid injection (group III); and nerve coaptation combined with HA film sheath and intramuscular VEGF gene in plasmid injection (group IV). Contralateral sciatic nerves were used as control. VEGF expression was verified from gluteal muscle biopsies surrounding the sciatic nerve by reverse transcriptase-PCR. Electrophysiological, histopathological, and electron microscopic evaluations were performed after 4 weeks. Mean peak amplitude of groups I–IV and nonoperated sciatic nerve were 4.5 ± 0.6 mV, 6.4 ± 0.4 mV, 6.7 ± 0.5 mV, 8.5 ± 0.4 mV, and 9.8 ± 0.5 mV, respectively. Mean myelinated axonal counts of groups I–IV and nonoperated sciatic nerve were 105 ± 24, 165 ± 19, 181 ± 22, 271 ± 23, and 344 ± 17, respectively. Treatment with HA film sheath coverage combined with intramuscular VEGF gene in plasmid injection yielded statistically significant higher peak amplitudes and myelinated axonal counts (P < 0.001). In addition, significantly less scar formation with HA administration (groups II and IV; P < 0.001) was found. Thus, it was found that VEGF might crucially regulate nerve regeneration processes and that HA can reduce the scar formation. This study showed that the combination of HA film sheath and VEGF gene may synergistically promote peripheral nerve regeneration. © 2013 Wiley Periodicals, Inc. Microsurgery, 2013.
    Full-text Article · Mar 2014 · Microsurgery
  • Article · Oct 2013 · International Journal of Experimental Pathology
  • Article · Aug 2013 · Respiratory medicine
  • Article · Jun 2013 · International Journal of Experimental Pathology
  • [Show abstract] [Hide abstract] ABSTRACT: Lumbar epidural varices are rare and usually mimick lumbar disc herniations. Back pain and radiculopathy are the main symptoms of lumbar epidural varices. Perineural cysts are radiologically different lesions and should not be confused with epidural varix. A 36-year-old male patient presented to us with right leg pain. The magnetic resonance imaging revealed a cystic lesion at S1 level that was compressing the right root, and was interpreted as a perineural cyst. The patient underwent surgery via right L5 and S1 hemilaminectomy, and the lesion was coagulated and removed. The histopathological diagnosis was epidural varix. The patient was clinically improved and the follow-up magnetic resonance imaging showed the absence of the lesion. Lumbar epidural varix should be kept in mind in the differential diagnosis of the cystic lesions which compress the spinal roots.
    Article · Jun 2013 · Asian spine journal
  • Article · May 2013 · Annals of Hematology
  • Article · May 2013 · Biochemical and Biophysical Research Communications
  • [Show abstract] [Hide abstract] ABSTRACT: Adequate lymph node evaluation is required for proper staging of colorectal cancer, and the number of lymph nodes examined is associated with survival. In this study, we aimed to evaluate the factors affecting the number of lymph nodes retrieved from specimens of patients operated for colorectal cancer. Medical records of 320 consecutive patients with colorectal cancer were evaluated retrospectively whom had curative resection between 2002 and 2007. Variables such as age, gender, tumor localization, depth of tumor invasion, number of lymph nodes retrieved, specimen length, stage and grade of disease, type of surgery, primary/recurrence disease' presence of preoperative chemo radiotherapy (CRT), surgeon, staff surgeon, pathologist, and staff pathologist were recorded and the results were evaluated statistically. Mean number of lymph nodes retrieved was 14.98 (0 to 129) and mean metastatic lymph node number was 2.37(0-25). Tumor localization, staging, primary/recurrence disease, length of specimen, type of operation, pathologist (resident pathologist), staff pathologist, staff surgeon, presence of CRT, affected statistically significiant in terms of lymph nodes harvested (p≤0.05). The hypothesis that disease recurrence occurred due to inaccurate staging. Maximal attention should be paid while doing oncologic surgery and should be paid to the total number of lymph nodes retrieved.
    Article · Mar 2013 · Gulhane Medical Journal
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    [Show abstract] [Hide abstract] ABSTRACT: : Many studies have shown that skin tissue extracted by circumcision can cause differences in sexual function, especially at the time of ejaculation. Sensitivity changes in penile skin and sexual satisfaction deriving from circumcision starting from premature ejaculation (PE) are discussed. Furthermore, most of these studies rely on questionnaires. Extracted free nerve endings (FNE) on the foreskin, which can detect temperature, mechanical stimuli (touch, pressure, stretch) or pain (nociception), have not been researched. Our aim is to determine FNEs in foreskin and the affects on sexual function, especially PE. This prospective study was done on adults who voluntarily applied to be circumcised between September 2010 and October 2011. The ejaculation latency times (ELT) before circumcision have been assessed, and a PE diagnostic tool (PEDT) form was filled out by the urologist according to the answers given by the volunteers. The proximal and distal ends of the foreskin were marked before circumcision, and the extracted foreskin was sent to the pathology department to determine FNEs. Twenty volunteers (average age 21.25 ± 0.44 years) were included in the study. The average ELT was 103.55 ± 68.39 seconds, and the average PE score was 4.35 ± 3.13. Proximal, middle, and distal tip nerve densities were compared. Proximal and distal (P / .003) and proximal and middle (P = .011) segments differed from each other, whereas middle and distal were similar (P = .119). There were not any correlations between PEDT scores and total nerve endings number (r = .018, P = .942). Also there were not any correlations between mean ELT and PEDT scores (r = .054, P = .822). The tissue extracted by circumcision has intensive FNEs, yet FNE intensity has no relation to PE.
    Full-text Article · Nov 2012 · Journal of Andrology
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    Zafer Demirer · Murat Zor · Bulent Kurt · [...] · Ibrahim Yildirim
    [Show abstract] [Hide abstract] ABSTRACT: Objective: To report the first case of a bilateral renal solitary fibrous tumor (SFT) as a metastasis of an inguinal malignant SFT. Clinical presentation and intervention: A 60-year-old male patient with a history of a right inguinal 7 × 8 cm soft tissue mass excision 9 years ago was referred to our clinic with abdominal pain. Both physical examination and chest X-ray were normal. Computed tomography revealed bilateral renal tumor. He was successfully treated with left partial and right radical nephrectomy. Histopathological examination showed a metastasis of the previous inguinal SFT. Conclusion: This case showed that although malignant SFT is extremely rare in the urogenital tract, this tumor should be included in the differential diagnosis when identified in the kidneys.
    Full-text Article · Aug 2012 · Medical Principles and Practice