[Show abstract][Hide abstract] ABSTRACT: This study aimed to observe the possible protective effects of resveratrol (RSV) against damage induced by di-n-butylphthalate (DBP), on the ductus epididymis and deferens in rats.
SIX GROUPS OF RATS WERE USED IN THE EXPERIMENT: Group 1: Control group; Group 2: Solvent (carboxymethylcellulose (CMC), 10ml/kg); Group 3: 500 mg/kg/day DBP; Group 4: 500 mg/kg/day DBP+20 mg/kg/day RSV; Group 5: 1000 mg/kg/day DBP; Group 6: 1000mg/kg/day DBP + 20 mg/kg/day RSV. Groups were treated by gavage for 30 days. Immunohistochemical, electronmicroscopic and histomorphometric examinations were carried out in the epididymis and deferens.
In the ductus epididymis and deferens mitochondrial crystolysis, exfoliation of the stereocilia and openings in lateral surface increased with DBP dosage, but these structures were recovered with RSV. DBP reduced the epithelial height of epididymis and vas deferens. Lumen dilatation was observed in both tissues. These disorders may lead to dysfunction of epithelial absorption. In the TUNEL examinations in both tissues, there were no apoptotic cells or apoptotic bodies.
In conclusion, DBP administration caused structural degeneration in the epididymis and deferens, parallel to dose evaluation and RSV can reverse these changes with its protective effects.
Full-text · Article · Mar 2014 · Indian Journal of Pharmacology
[Show abstract][Hide abstract] ABSTRACT: Objective: Hyperthermia is the condition of the body temperature rising at 41°C or more. Heat stres on ductus epididymis leads to abnormal spermium production and spermatogenesis. Hyperthermia induced oxidative stres and apopitosis can be blocked by superoxide dismutase (SOD). In this study we aimed to determine the protective effects of SOD on ductus epididymis at hyperthermic conditions by using apoptotic and oxidative stress markers Methods: Wistar Albino male rats were divided into seven groups. Every group has 6 rats. Control group had set for 20 minutes in a pool which was 22°C temperature. On the second, third, fourth groups NaCl + catalase were applied one hour before hyperthermia, and then the rats were abondoned in 42°C water bath for 20 minutes. After that tissue were taken at 6th, 24th, 72th hours respectively. On the fifth, sixth and seventh groups NaCl + catalase + SOD was applied one hour before hyperthermia and then the rats were abandoned in 42" C water bath for 20 minutes. After hyperthermia for 20 minutes tissues were taken at 6th, 24th and 72 th hours respectively. In order to determine apoptosis caused by hyperthermia, removed tissues were subjected to indirect immunohistochemical method with HSP-70 primary antibodies to detect impact on Caspase 9, Caspase 8 and Caspase 3 protein structures. Results: Morfologically abnormal spermium including lumens show positive Caspase-3 reaction. This was accepted as an indicator of abnormal spermiums, which were caused by effects of hyperthermia, can reach ductus epididymis. It was also remarkable that some areas, round spermatide heads directed into epithelium an even intruded into stereocilias. It was thought that, this direction can be caused by a mechanism which is organized by principle cells, which were not destroyed in Sertoli cells. It was observed that, for all caspases, SOD application is reducing the immunreactivity in parallel to time. Conclusion: The scrotal temperature elevation shows its harmfull effects on epididymis, but the SOD application may suppress apoptosis in parallel to time by increasing HSP70 level.
No preview · Article · Jan 2014 · Gazi Medical Journal
[Show abstract][Hide abstract] ABSTRACT: Objective: The aim of this study is to determine the ultrastructural effect of fasting and applications of histamine and gastrin after fasting in gastric parietal cell. Methods: In this study, nine groups were composed of Swiss albino male rats. Group 1: Control, group 2: fasting for 12 hours, group 3: 12 hours fasting +1hour histamine, group 4: 12 hours fasting + 3min. gastrin, group 5: 12 hours fasting + 3min. DMSO (solvent of gastrin), group 6: 12 hours fasting + 7min. gastrin, group 7: 12 hours fasting + 7min. DMSO, group 8: 12 hours fasting + 15min. gastrin, group 9: 12 hours fasting + 15min. DMSO. At the end of the study time, the tissue samples were passed through routine electron microscopic preparation methods and tissues were embedded in araldite CY212 kit. Thin sections were evaluated on Carl Zeiss EM 900 electron microscope. Results: It was determined in the executed research, by using the electron microscope, that tubulovesicular structures were increased in the fasting group independently of the control group, vacuolar structures were also observed in patches and histamine application did not reveal any difference except for mitochondrial differences in small amounts. However, it was determined that gastrin application increased the cellular degeneration parallel to increasing duration. This transformation was especially observed in nuclear structures, mitochondria and intracytoplasmic canaliculi in the cells. Conclusion: The distinct degeneration which is observed during the gastrin application in this study was evaluated as a result of the augmentation of the acid secretion by gastrin using two ways.
No preview · Article · Sep 2011 · Gazi Medical Journal
[Show abstract][Hide abstract] ABSTRACT: The aim of this study was to evaluate the application of hematoxylin, safranin, light green and picric acid as counterstain in sperm immunohistochemistry. This is important to visualize the best staining procedure and to determine the advantage of picric acid as a counterstain in some situations. Picric acid used for counterstaining in the immunohistochemical procedure gives the best image of reaction on sperms when DAB was used as a chromogen.
No preview · Article · Jul 2009 · Archives of Andrology
[Show abstract][Hide abstract] ABSTRACT: Purpose: To investigate ultrastructural changes in different age groups, from birth to adult ages and in the senescence period. Methods: Fifteen Wistar albino rats were used, in five groups (group 1: newborn; group 2: 5 weeks old; group 3: 7 weeks old; group 4: 3 months old; group 5: 1 year old), with five animals in each. Semi-thin sections were stained with Toluidine blue and evaluated under a photo-light microscope after routine electron microscopic processing had been done. Thin sections were investigated and estimated by Carl Zeiss EM 900. Results: Three types of cell were determined in the olfactory epithelium in all groups. Lamina propria was a loose connective tissue that included blood vessels, connective tissue fibers, and Bowman's glands. We did not observe any degenerative changes in the newborn or young groups, although considerable degeneration in the cilia and microvilli of olfactory neurons and sustentacular cells was seen in the elderly group's olfactory mucosa. Apoptotic figures and cells were seen in the elderly group also. Conclusion: Senescence is a complicated period that decelerates the activities of organelles, tissues, and cells. For this reason, the degeneration and changes determined in our study in olfactory mucosa were considered normal.
No preview · Article · Mar 2008 · Gazi Medical Journal
[Show abstract][Hide abstract] ABSTRACT: Purpose: To analyze the distribution of EGF receptors (EGF-R) in the uterine tubes of rate at various phases of the estrous cycle immunohistochemically. Methods: Rat uterine tube tissue samples from certain phases of the menstrual cycle were processed for paraffin embedding. Indirect immunohistochemical and EGF-R primary antibody analyses were performed on the slides. Afterwards, they were examined by Olympus BH2 photo-light microscope. Results: In uterine tubes, EGF-R expression was not detected in the tuba uterine muscle layer or vessel wall in any estrous phase, but in the other parts its level varied in these phases. At proestrous, EGF-R was homogeneous in the epithelial cell cytoplasm, denser in the apical cell membrane, low in serosa layer mesothelial cells' external cell surface and ranged from low to denser in connective tissues. At estrous, however, EGF-R expression was higher in the apical cytoplasm of cilial cells on the upper surface of mucous folding, but low in the lamina propria. Expression was detectable in some cells near the vessel wall within connective tissue of serosa, but was denser in the mesothelia. At metacstrous, beside higher expression in both the apical cytoplasm and cell membrane of epithelia and some cells in lamina propria, no immunohistochemical staining was observed in serosa. At diestrous, expression in the epithelia layer and in the cell membrane was denser and low in serosa. Conclusion: Blood estrogen level dependent EGF-R expression was determined in the tuba uterine, and, in accordance with increased blood estrogen levels, EGF-R expression was stimulated.
No preview · Article · Mar 2008 · Gazi Medical Journal
[Show abstract][Hide abstract] ABSTRACT: To comparatively analyze the ultrastructural changes in the submandibular and parotid glands and in the exocrine pancreas following diabetes induced by Streptozotocin exposure and the effects of fasting and insulin treatment on these alterations.
For experimental procedure, we included 48 Sprague-Dawley type rats in July 2001-March 2002 at Gazi University, Turkey. We divided the rats into 8 groups following the infusion of Streptozotocin.
While the degeneration manifested itself as accumulation of secretions within the mucous cells in the submandibular gland, lipid droplets were absent, being replaced by vacuolar structures. The parotid gland and exocrine pancreas, having similar properties, were affected similarly. Diabetes-induced loss of granules was observed in the serous cells in both glands. There was diffuse lipid accumulation within these cells. Regarding granule content, we observed the most prominent degenerative changes in the parotid gland. While cellular loss was observed in neither the submandibular, nor the parotid gland, we noted presence of apoptotic cells was noted in the pancreas. State of fasting was found to cause alterations within the glands indicating increased activity. While insulin treatment was seen to restore the structure to normal in general in both of the 3 glands.
This study demonstrated that both of the 3 glands are affected by diabetes and concomitant fasting, and this effect manifests itself via the granule content.
Preview · Article · Feb 2007 · Saudi medical journal
[Show abstract][Hide abstract] ABSTRACT: This study was intended to investigate the effects of alcohol on the ultrastructure of fetal cerebellar Purkinje cells. Twelve adult female rats of Sprague-Dawley species were utilized. Control and experiment groups were formed. Rats were made pregnant. Rats in experiment group were administered liquid diet containing 6% alcohol. Cerebellums of infant rats were taken on 6th, 8th, and 10th days after birth. For electron microscopy, tissue sections were processed and stained with the usual methods. When control and experiment groups were compared for electron microscopic investigation, degeneration of mithocondria as cristolysis, dilatations of rough endoplasmic reticulum tubuli, and ring-shaped appearance of Golgi apparatus unit were determined. In some groups, nuclear membrane disintegrated. In cytoplasms of Purkinje cells, multivesicular bodies were distinguished. It was determined that liquid diet containing 6% alcohol had toxic effects on Purkinje cells and caused ultrastructural signs of degeneration in these cells.
No preview · Article · Jan 2006 · International Journal of Neuroscience
[Show abstract][Hide abstract] ABSTRACT: The effects of various estrogen replacement protocols to prevent bone loss following ovariectomy have been the subject of many studies in rats. This study was designed to determine the effects of early intermittent high-dose estrogen replacement therapy, which has hitherto not been studied, on bone structure of ovariectomized rats.
Bilateral ovariectomies were performed in 20 female mature non-pregnant Wistar rats. All the animals were randomly assigned to two groups to receive either subcutaneous 17 beta-estradiol (25 mg/kg) or only sesame oil on days 15 and 22 after ovariectomy. Fourteen days after the last injection, the rats were sacrificed and proximal femurs were removed for both light and electron microscopic analyses.
In the light microscopic analysis, control femurs exhibited a marked destruction in the structure of the cancellous bone, whereas estradiol-treated rats had almost normal cancellous bone. Ultrastructural analysis showed degeneration and increased turnover in bone cells of the control femurs, whereas the bone cells and the bone matrix appeared almost normal in the treatment group. A statistically significant increase in serum estrogen levels was found in estradiol-treated rats (580+/-124 pg/ml versus 62+/-16 pg/ml, p<0.001).
Intermittent high-dose estrogen treatment prevents cancellous bone loss in the proximal femurs of ovariectomized rats through inhibition of bone turnover and results in significantly increased serum estrogen levels.
Preview · Article · Feb 2003 · acta orthopaedica et traumatologica turcica
[Show abstract][Hide abstract] ABSTRACT: The distribution of EGF receptors (EGF-R) was examined in normal, hyaline membrane diseased and pneumonic newborn lung tissues by immunohistochemical methods under the light microscope. The PAP technique with polyclonal antibodies was performed to demonstrate the EGF receptor localisation in these tissues. Strong EGF-R reactivity was observed on bronchiolar epithelium and type I and type II alveolar cells in normal newborn lung tissues; whereas, poor reactivity was observed in alveolar macrophages. On the other hand, strong immunoreactivity was detected in type I alveolar cells and alveolar macrophages in hyaline membrane disease, but no reactivity was present in type II alveolar cells. The strongest immunoreactivity was observed in alveolar macrophages of newborn pneumonic lung tissues. In conclusion, the most meaningful form of reactivity was observed in normal newborn lung tissues of airway track and respiration area. This result is related with the maturation of the lungs after birth.
No preview · Article · Feb 2001 · Acta Physiologica Hungarica
[Show abstract][Hide abstract] ABSTRACT: The fetal skeleton double staining method is used to reveal developmental abnormalities in the skeletal system. We used alizarin red S and alcian blue successfully with microwave irradiation for skeletal double staining. The fixation time was reduced from 4-7 days to 2-2.5 min and the staining time was reduced from 4 days to 23 min.
No preview · Article · Apr 1999 · Biotechnic and Histochemistry
[Show abstract][Hide abstract] ABSTRACT: The integrin receptors are a family of transmembrane glycoproteins comprising non-covalent heterodimers. They interact with a wide variety of ligands including extracellular matrix glycoproteins, complement and other cell, while their intracellular domains interact with the cytoskeleton. They participate in cell-matrix and cell-cell adhesion in many physiologically important processes including embryological development, hemostasis, thrombosis, wound healing, immune and nonimmune defense mechanisms, and in oncogenic transformation. This investigation was focused on the histological distribution of the beta 1-integrins in the human tonsil using an indirect immunoperoxidase method. Present data suggest that lymphocyte and antigen presenting cells (FDCs, IDCs, and macrophages) interact with each other following adhesion to extracellular matrix proteins (e.g. fibronectin) through their integrin receptors in order to carry out special immunological functions. In addition, stromal elements and epithelial components were shown to express VLA integrins providing interactions for tissue organization and compartmentalization.
No preview · Article · May 1997 · Annals of Anatomy - Anatomischer Anzeiger
[Show abstract][Hide abstract] ABSTRACT: The integrin receptors are a family of transmembrane glycoproteins comprising non-covalent heterodimers. They interact with a wide variety of ligands including extracellular matrix glycoproteins, complement and other cells while their intracellular domains interact with the cytoskeleton. They participate in cell-matrix and cell-cell adhesion in many physiologically important processes including embryological development, hemostasis, thrombosis, wound healing, immune and nonimmune defense mechanisms, and oncogenic transformation. This investigation is focused on the histological distribution of the beta 1-integrins in the human thymus, using an indirect immunoperoxidase method. With the exception of VLA-4, none of the beta 1 integrins were expressed on thymocytes which were strongly positive in the cortex and perivascular compartment, somewhat weaker in the medulla. Thymic epithelial cells were positive for VLA-1, VLA-2, VLA-3 and VLA-6, but the distribution pattern of these molecules in epithelial cells at certain locations was quite different. VLA-1 was weakly expressed by both cortical and medullary epithelial cells. VLA-2 was strongly positive in cortical epithelial cells forming a dense framework at the peripheral cortex. VLA-3 and VLA-6 selectively stained a single flattened epithelial cell layer (perilobular epithelial cells) demarcating the peripheral cortex from the surrounding perivascular compartment. VLA-1,3,5,6 were also demonstrated in the endothelial cells and subendothelial layer of the thymic vasculature. In conclusion: the distribution of integrins in human thymus tissues is of special interest. Such distribution shows that the VLA integrins may have different functions in different areas. The data presented in this study may be important in evaluating the functional role of the VLA integrins in thymocyte maturation in different compartments of the thymus.
No preview · Article · Feb 1996 · Annals of Anatomy - Anatomischer Anzeiger