[Show abstract][Hide abstract] ABSTRACT: Sub-fractions of all apo B-100 containing lipoproteins (low density lipoproteins, very low density lipoproteins and intermediate density lipoproteins) with reduced contents of sialic acid were found in vivo in human blood. These lipoproteins were inclined to spontaneously form aggregates and were able to stimulate accumulation of cholesterol in cells cultured from human aortic intima. In vitro treatment of apo B-containing lipoproteins with 2,6- and 2,3-specific sialidases, alpha-mannosidase, endoglycosidases F1 or F2 or peptide-N-glycanase F also stimulated aggregation and increased the ability of these particles to potentiate cholesterol accumulation in cells of the intact human aortic intima. So, deglycosylation of various apo B-containing lipoproteins possibly occurs in the blood, decreases their resistance to aggregation and increases the ability of these particles to stimulate accumulation of cholesterol in human aortic intima cells, thereby increasing their atherogenic potential.
No preview · Article · Jan 2009 · Archives of Physiology and Biochemistry
[Show abstract][Hide abstract] ABSTRACT: Binding of low-density lipoproteins to elastins from the media and proteoglycan and muscle sublayers of human intact and atherosclerotic
aorta was studied. Circulating modified lowdensity lipoproteins intensively bound to elastin due to the loss of sialic acid.
No preview · Article · Jan 2000 · Bulletin of Experimental Biology and Medicine
[Show abstract][Hide abstract] ABSTRACT: Blood serum of patients with documented coronary atherosclerosis induces intracellular cholesterol accumulation in a primary
culture of human aortic intimal cells. Serum prepared from blood collected 2–4 h after administration of a tablet containing
300 mg garlic powder (Kwai, Germany) induces a much lower cholesterol accumulation in cultured cells. A tablet with the same
amount of garlic but with a prolonged effect (Allikor, Russia) lowers serum atherogenicity for 12–14 h. The direct antiatherogenic
effect of garlic powder is confirmed by cell culture experiments with aqueous extracts from garlic powder.
No preview · Article · Jul 1996 · Biulleten' eksperimental'noĭ biologii i meditsiny