Tohru Tezuka

Teikyo University, Edo, Tōkyō, Japan

Are you Tohru Tezuka?

Claim your profile

Publications (31)57.52 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Several essential oils possess pharmacological effects. Among the various constituents of essential oils, 1, 8-cineole has been shown to possess pharmacological effects such as anti-bacterial and anti-inflammatory effects. The effect of 1, 8-cineole on human colorectal cancer cells, however, has not reported previously. In this study, we have investigated the anti-proliferative effect of 1, 8-cineole on human colon cancer cell lines HCT116 and RKO by WST-8 and BrdU assays. The cytotoxicity of 1, 8-cineole was investigated by LDH activity and TUNEL staining. The mechanism of apoptosis by 1, 8-cineole was determined by western blot analyses. In in vivo study, RKO cells were injected into the SCID mice and the effect of 1, 8-cineole was investigated. Specific induction of apoptosis, not necrosis, was observed in human colon cancer cell lines HCT116 and RKO by 1, 8-cineole. The treatment with 1, 8-cineole was associated with inactivation of survivin and Akt and activation of p38. These molecules induced cleaved PARP and caspase-3, finally causing apoptosis. In xenotransplanted SCID mice, the 1, 8-cineole group showed significantly inhibited tumor progression compared to the control group. These results indicated 1, 8-cineole suppressed human colorectal cancer proliferation by inducing apoptosis. Based on these studies 1, 8-cineole would be an effective strategy to treat colorectal cancer.
    No preview · Article · Oct 2013 · Oncology Reports
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: Continuous treatment with FOLFOX therapy is associated with peripheral nerve toxicity, and to improve this inconvenient side effect various methods of administration are being investigated. A regimen of intermittent oxaliplatin administration by continuous infusion therapy, i.e., modified FOLFOX7 (mFOLFOX7) + bevacizumab, was designed with the goal of alleviating severe peripheral nerve disorders and hematological toxicity. A phase II clinical study was conducted to evaluate the efficacy and safety of this regimen. Methods: Previously untreated patients were assigned to mFOLFOX7 (oxaliplatin 85 mg/m(2), levofolinate [l-LV] 200 mg/m(2), 5-fluorouracil [5-FU] 2400 mg/m(2)) + bevacizumab (5 mg/kg) administered every 2 weeks for 8 cycles, maintenance without oxaliplatin for 8 cycles, and reintroduction of mFOLFOX7 + bevacizumab for 8 cycles or until disease progression. Progression free survival (PFS) following the first dose (PFS 1) and following reintroduction of oxaliplatin (PFS 2) were used as indices for assessing the efficacy of intermittent administration. Results: Fifty-two patients were enrolled, with median age of 64 years (range, 36-74). Median PFS 1 was 11.8 months (95 % confidence interval [CI], 9.5 to 13.7), median time to treatment failure was 10.3 months (95 % CI, 5.6 to 12.1), percentage of patients with neutropenia of grade 3 or higher was 7.8 %, and percentage with peripheral nerve disorders was 3.9 %. Response rate was 50 %, and 84.4 % of patients who started modified simplified LV5FU2 + bevacizumab were reintroduced to oxaliplatin. Conclusion: By excluding 5-FU bolus administration and administering bevacizumab continuously the mFOLFOX7 + bevacizumab regimen with preplanned withdrawal of oxaliplatin showed high tolerability and prevented severe peripheral neuropathy and neutropenia without reducing efficacy.
    Full-text · Article · Jul 2013 · Investigational New Drugs
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Fatty acid synthase is highly expressed in many types of human cancers. Cerulenin, a natural inhibitor of fatty acid synthase, induced apoptosis in the human colon cancer cell lines HCT116 and RKO. Oxaliplatin also induced cell death in these cell lines. Cerulenin treatment was associated with reduced levels of phosphorylated Akt, activation of p38 and induced caspase-3 cleavage and finally caused apoptosis. Oxaliplatin induced activation of the p53-p21 pathway and p38. In combination with cerulenin and oxaliplatin, activation of the p53-p21 pathway and p38 occurred in a smaller concentration and finally induced caspase-3 cleavage in a smaller concentration of cerulenin and oxaliplatin. In xenotransplanted SCID mice, the cerulenin + oxaliplatin group significantly inhibited tumor progression compared to the control, cerulenin and oxaliplatin groups. Based on these studies, inhibiting fatty acid synthase would be an effective strategy to treat unresectable colorectal cancer tumors in combination with oxaliplatin. Fatty acid synthase inhibitor would be one of the best counterparts of oxaliplatin, which reduces the dose and side-effects of oxaliplatin and would make it possible to endure the chemotherapy over a longer period.
    Full-text · Article · Jun 2013 · International Journal of Oncology

  • No preview · Article · Jan 2013 · Nihon Gekakei Rengo Gakkaishi (Journal of Japanese College of Surgeons)

  • No preview · Article · Sep 2012 · European Journal of Surgical Oncology
  • [Show abstract] [Hide abstract]
    ABSTRACT: We evaluated the efficacy and safety of neoadjuvant chemotherapy using modified OPTIMOX1 plus bevacizumab for advanced rectal cancer. Nine cases with highly advanced rectal cancer for which curative surgery was potentially difficult were enrolled(clinical T4 in 7 cases, lateral node metastasis in 3 cases, M1 in 2 cases). The number of courses of modified OPTIMOX1(mFOLFOX6 and sLV5FU2, alternating administration)plus bevacizumab ranged from 1 to 21(median: 10). Surgical procedures consisted of internal sphincter resection(ISR)in 4 patients, ultra-low anterior resection(ULAR)in 2 patients, pelvic exenteration(TPE)in 2 patients, and Hartmann's procedure in 1 patient. Liver resection was conducted in 2 patients. RM1 was confirmed in 2 patients, but curative surgery was performed in the other patients. Histological efficacy of grade x/1a/1b/2were seen in the above 1/4/2/2 cases, respectively. Neurotoxicity associated with oxaliplatin was mild; no grade 3 neurotoxicity was noted. Recurrence has been confirmed in 5 patients at the median follow-up period of 650 days. It was suggested that modified OPTIMOX1 plus bevacizumab is effective and safe to administer as a neoadjuvant chemotherapy for curative resection or anus-preserving surgery in patients with highly advanced rectal cancer.
    No preview · Article · Jul 2012 · Gan to kagaku ryoho. Cancer & chemotherapy
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: The aim of this study was to clarify the management of acute colon and rectum obstruction caused by colorectal carcinoma using a trans-anal and a naso-intestinal drainage tube before surgery. Method: Forty colorectal cancer patients (26 men and 14 women) with acute large-bowel obstruction were treated in our department during the last three years. Three patients were treated with naso-intestinal tube and the trans-anal tube drainage was performed in 24 cases before surgery. The surgical outcomes of these patients were analyzed. Results: The site of obstruction was cecum in 1 patient, ascending colon in 4, transverse colon in 6, descending colon in 7, sigmoid colon in 8, and rectum in 14. Three patients (27%) among 11 right-sided colon cancer obstruction (RCCO) were treated by inserting a long naso-intestinal tube. Two cases (18%) were treated with a trans-anal drainage tube. Twenty two patients (75%) with left-sided colorectal cancer patients (LCCO) were treated with a trans-anal drainage tube. One patient of LCCO was combined with a long naso-intestinal tube. Two patients were Stage IV in RCCO (18%), whereas 17 patients of LCCO were stage IV (58%). All of RCCO were performed standard colectomy, whereas 9 patients (31%) of LCCO were treated with simple colostomy without removing cancer and 8 patients (28%) were treated with Hartmann’s procedure. Surgical site infection occurred in 3 patients of RCCO and 7 patients of LCCO. In LCCO, postoperative ileus occurred in 5 patients and anastomotic leakage in 3 patients to whom preoperative drainage tube was not inserted. Conclusions: Acute bowel-obstruction was more common in left-sided advanced colorectal cancer. Management of acute colorectal obstruction using either the naso-intestinal or trans-anal drainage tube was found to be effective and safe, considered as a bridged method before curative surgery.
    No preview · Conference Paper · Jun 2012

  • No preview · Article · Jan 2012

  • No preview · Article · Jan 2012 · Nihon Rinsho Geka Gakkai Zasshi (Journal of Japan Surgical Association)

  • No preview · Article · Dec 2011 · International Journal of Colorectal Disease

  • No preview · Article · Apr 2011 · Cancer Research

  • No preview · Article · Jan 2011 · Suizo
  • [Show abstract] [Hide abstract]
    ABSTRACT: The objective of this study was to clarify the surgical outcome of patients with palliative surgery for malignant bowel obstruction. This study investigated the clinical features, operative procedures and postoperative course of 35 patients who underwent a palliative surgery for malignant bowel obstruction. And then the patients were divided into two groups; Patients in A group were consisted of 4 patients with hospital death and 7 patients with postoperative complications. Patients in B group were consisted of 24 patients without hospital death or postoperative complications. Eighteen patients who had been inserted nasogastric tube or ileus tube in the preoperative state could be removed. Thirty-three of 35 patients(94.3%)could become an oral ingestion. Four of 35 patients(11.4%)could not be discharged; 3 patients died of cancer and 1 patient died of acute myocardial infarction. Postoperative complications were seen in 7 patients except 4 patients with hospital death. The median postoperative stay was 18 days(3-58). Twenty six of 35 patients(74.3%) underwent chemotherapy. The median survival time was 137 days(3-1,614). The patients in A group showed a lower level of albumin(p=0.0071)and hemoglobin,(p=0.0006)and poorer performance status(p=0.0178)than the patients in B group. The median hospital stay of the patients in A group and B group were 28 days and 16 days, respectively(p=0.0823). The median survival time of the patients in A group and B group were 42 days and 119 days, respectively(p=0.0035). We concluded that the palliative surgery made an oral ingestion possible and improved a quality of life of the patients with malignant bowel obstruction. However, the surgical indication should be carefully decided for the patients with low albumin, hemoglobin and poor performance status.
    No preview · Article · Dec 2010 · Gan to kagaku ryoho. Cancer & chemotherapy
  • [Show abstract] [Hide abstract]
    ABSTRACT: Incidental detection of pancreatic tumors has in recent years become more frequent owing to progress made in diagnostic imaging. We report a case of a preoperatively diagnosed intrapancreatic accessory spleen performed limited surgery for not being able to ruled out malignancy of cystic component. A 55-year-old woman with suspected appendicitis underwent computed tomography which detected a cystic lesion with a solid component 3cm in diameter at the pancreatic tail. We suspected the tumor to be an intrapancreatic accessory spleen because endoscopic ultrasonography showed identical image patterns for both the solid component of the tumor and spleen. Superparamagnetic iron oxide magnetic resonance imaging showed a decrease in signals, and 99mTc-Sn colloid scintigraphy indicated an uptake in both lesions. The patient was given a diagnosis of intrapancreatic accessory spleen, and informed that malignancy could not be ruled out completely upon which she requested surgery. The tumor at the pancreatic tail was soft, brownish, and 3cm in diameter, and spleen preserving pancreatic tail resection was performed confirming the diagnosis of intrapancreatic accessory spleen by intraoperative frozen section. The postoperative course was uneventful and she was discharged on postoperative day 16. To the best of our knowledge only 9 cases of preoperatively diagnosed intrapancreatic accessory spleen have been reported in the literature, and we therefore report this rare case.
    No preview · Article · May 2010 · Nippon Shokaki Geka Gakkai zasshi
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We investigated the feasibility of new carbon dioxide-enhanced virtual multidetector computed tomography (MDCT) cholangiopancreatography (CMCP) for intraluminal exploration in 73 patients with hepatobiliary and pancreatic disease. CMCP was performed via a percutaneous or transpapillary drainage tube, and, synchronously, intravenous contrast material was employed for virtual angiography; three-dimensional (3D) virtual reality was incorporated using OsiriX and Fovia applications. The capability of carbon dioxide to delineate the biliary and pancreatic system was evaluated. All CMCPs showed complete technical success; complications including pancreatitis or pain never occurred. The incidences of visible third- and fourth-order biliary branches were 100 and 86.0%. The capability of carbon dioxide to pass an obstruction through an occluded hilar bile duct malignancy was 80.0%; it provided feasible information on additional bile duct segments. The full extent of the gallbladder was depicted in 72.7% of the studies. Minimum 2-mm lesions of biliary stones or gallbladder polyps were clearly detected. The main or second-order pancreatic ducts were visible in 100 and 83.3% of the studies, respectively. Carbon dioxide enabled the replacement of mucin and pancreatic juice and facilitated the detection of cystic lesions of intraductal papillary-mucinous neoplasm (IPMN) in 75.0% of the studies. We succeeded in achieving 3D spatial recognition of vascular structures in the cholangiopancreatic region, through the fusion of CMCP and 3DCT arteriography and venography in a single image scanning, and radiation time was decreased. This combined modality proved to be feasible for planning operations and for image-guided navigated surgery in the resection of a malignancy. To our knowledge, this is the first report to demonstrate the diagnostic accuracy of carbon dioxide MDCT cholangiopancreatography and the use of this modality for depicting biliary, pancreatic, and fusion blood vessels simultaneously. Carbon dioxide possesses many advantages over conventional iodinated contrast agents, and it might replace more invasive diagnostic measures in the near future.
    Preview · Article · Oct 2009 · Journal of Hepato-Biliary-Pancreatic Sciences
  • [Show abstract] [Hide abstract]
    ABSTRACT: We applied a new concept of "image overlay surgery" consisting of the integration of virtual reality (VR) and augmented reality (AR) technology, in which dynamic 3D images were superimposed on the patient's actual body surface and evaluated as a reference for surgical navigation in gastrointestinal, hepatobiliary and pancreatic surgery. We carried out seven surgeries, including three cholecystectomies, two gastrectomies and two colectomies. A Macintosh and a DICOM workstation OsiriX were used in the operating room for image analysis. Raw data of the preoperative patient information obtained via MDCT were reconstructed to volume rendering and projected onto the patient's body surface during the surgeries. For accurate registration, OsiriX was first set to reproduce the patient body surface, and the positional coordinates of the umbilicus, left and right nipples, and the inguinal region were fixed as physiological markers on the body surface to reduce the positional error. The registration process was non-invasive and markerlesss, and was completed within 5 min. Image overlay navigation was helpful for 3D anatomical understanding of the surgical target in the gastrointestinal, hepatobiliary and pancreatic anatomies. The surgeon was able to minimize movement of the gaze and could utilize the image assistance without interfering with the forceps operation, reducing the gap from the VR. Unexpected organ injury could be avoided in all procedures. In biliary surgery, the projected virtual cholangiogram on the abdominal wall could advance safely with identification of the bile duct. For early gastric and colorectal cancer, the small tumors and blood vessels, which usually could not be found on the gastric serosa by laparoscopic view, were simultaneously detected on the body surface by carbon dioxide-enhanced MDCT. This provided accurate reconstructions of the tumor and involved lymph node, directly linked with optimization of the surgical procedures. Our non-invasive markerless registration using physiological markers on the body surface reduced logistical efforts. The image overlay technique is a useful tool when highlighting hidden structures, giving more information.
    No preview · Article · Oct 2009 · Journal of Hepato-Biliary-Pancreatic Sciences
  • [Show abstract] [Hide abstract]
    ABSTRACT: Transgastric access is a major route in natural orifice translumenal endoscopic surgery (NOTES); gastrotomy should be performed unless it would damage surrounding organs in the peritoneal cavity. This article describes a novel rendezvous gastrotomy technique over a direct percutaneous endoscopic gastrostomy (PEG). In six live porcines, the gastrotomy involved applying a direct PEG through the abdominal wall into the stomach and exchanging to a needle trocar. An endoscopic balloon catheter was passed through the trocar by rendezvous technique. Then the inflated balloon and endoscope were advanced to the peritoneal cavity through the gastrotomy. Transgastric cholecystectomy was performed with a hybrid needle grasper through the same percutaneous site and the gastrotomy was closed with endoscopic clips. The rendezvous gastrotomy technique could reduce guidewire exchange. The success rate was 100% (6/6). Mean times for transgastric peritoneoscopy and cholecystectomy were 25.5 and 83.5 min. Mortality and morbidity was 0%. The addition of the extra trocar was unnecessary in all procedures. The advantage of this introduction system includes the creation of controlled gastric perforation, which is easier to close. It provides reliable transgastric access and increases safety. It simplifies transgastric NOTES and provides less invasive hybrid NOTES procedure.
    No preview · Article · Aug 2009 · Journal of Hepato-Biliary-Pancreatic Surgery
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose : We clarified the feasibility of inguinal hernia surgery by junior residents. Materials & Methods : We analyzed 139 retrospective cases of radical inguinal hernia surgery between April 2005 and December 2007. Of these, 134 involved mesh-plug repair and 5 Prolene hernia repair. We retrospectively compared the recurrence rate, the rate of complications, blood loss volume, operating time, and the length of postoperative hospital stay between patients treated surgically by junior residents (group R, n=72) and those treated by surgical staff (group S, n=67) . Results : Total rates of recurrence and complication were 5.8% in group R and 8.6% in group S, respectively. Significant statistical differences were seen between groups in recurrence rate (group R, 9.7%; group S, 1.5% : p<0.05) and operating time (group R, 88 min : group S, 64 min ; p<0.001). No statistically significant difference was seen in blood loss volume, rate of complications, or the length of postoperative hospital stay. Conclusions : The incidence of short-term complications in inguinal hernia surgery conducted by junior residents was identical to that of surgical staff. The high recurrence rate seen in inguinal hernia surgery by junior residents evoked, however, the need to implement surgical treatment guidelines for junior residents.
    No preview · Article · Jun 2009 · Nippon Shokaki Geka Gakkai zasshi
  • [Show abstract] [Hide abstract]
    ABSTRACT: Natural orifice translumenal endoscopic surgery (NOTES) is a novel concept using an endoscope via a translumenal access for abdominal surgery. This study was designed to evaluate the feasibility and technical aspects of NOTES cholecystectomy from our experience on humans and animals. NOTES cholecystectomies were performed in 12 animal experiments, including 8 pigs (6 by transgastric and 2 by transvaginal accesses) and 4 dogs (4 transvaginal accesses), and a human female cadaver. The entire gallbladder could be removed under direct vision in all experiments. The average time was 60 min by transgastric and 40 min by transvaginal in animals. It was 87 min for human transvaginal cholecystectomy. In all animal and human procedures, there was no major complication concerning the operation. The transvaginal route may be the easiest route for abdominal NOTES. Percutaneous endoscopic gastrostomy (PEG) allowed the safe performance of a controlled gastric perforation and shortened the time. The hybrid method allowed performance of a safe procedure and shortened the time. Transvaginal and transgastric NOTES cholecystectomy is technically feasible and safe in both humans and animals. New instrumentation needs to be developed to perform a pure NOTES cholecystectomy without transabdominal assistance.
    No preview · Article · May 2009 · Journal of Hepato-Biliary-Pancreatic Surgery
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to examine correlations between pressure profile of the anal canal and postoperative defecatory disorder after sphincter-preserving operation (SPO) for rectal cancer. Using three-dimensional vector manometry, pressure profile and length of the anal canal were evaluated more than 1 year after SPO according to operation method and degree of postoperative defecatory function in 53 patients with rectal cancer. Compared with high anterior resection as a control, the anal canal was shorter in operations with a pelvic floor maneuver, namely, low anterior resection, ultra-low anterior resection, and intersphincteric resection. Patients with postoperative defecatory disorder showed significantly shorter anal canal length than patients with fair function. Length of the circular high-pressure zone (> or = 20 mmHg) < 20 mm in the resting state was a strong predictor of severe postoperative defecatory malfunction, with Wexner score> or =10. Operative maneuvers at the pelvic floor during SPO for rectal cancer may damage anal sphincter or levator ani muscles. The circular high-pressure zone can be measured only by three-dimensional manometry and may offer a useful indicator of sphincter damage after SPO for rectal cancer.
    No preview · Article · Apr 2009 · Journal of the American College of Surgeons