Tao-Ping Shi's scientific contributionswhile working at 307 Hospital of the Chinese People's Liberation Army (Beijing, China) and other institutions
- Abstract: We compared the clinical outcomes of retroperitoneoscopic and open adrenalectomy for pheochromocytoma. Clinical data on 56 patients who underwent retroperitoneoscopic lateral adrenalectomy were retrospectively compared with those on 50 who underwent open adrenalectomy for pheochromocytoma, including patient demographic data, perioperative indexes and clinical outcomes. Demographic data on patients were similar in the 2 groups. In the retroperitoneoscopic group such perioperative indexes were... Show More
- Abstract: To investigate the association between DNA polymorphisms, including single-nucleotide polymorphisms (SNPs) and insertion/deletion polymorphisms, in exon 1 and promoter of the CDH1 gene, and the risk of transitional cell carcinoma (TCC) of the urinary bladder (TCCB). This was a hospital-based case-control study of 180 patients with TCCB and 110 normal controls. Genomic DNA was extracted from blood samples of all participants and genotypes determined using polymerase chain reaction and DNA... Show More
- Abstract: We investigated the expression of Notch receptors and ligands in normal bladder transitional epithelium and transitional cell carcinoma of the bladder. We also explored its clinical and pathological implications. The expression of Notch-1 to 3, Jagged-1 and Delta-like-1 was detected respectively in 70 cases of bladder carcinoma, 10 of normal urothelium and the 2 cell lines T24 and BIU-87 using immunohistochemistry. Reverse transcriptase-polymerase chain reaction and Western blot were used to... Show More
- Abstract: We introduced a multimodal training program for laparoscopic pyeloplasty (LP) and evaluated its safety, feasibility, and efficacy. The program consisted of box-trainer training, animal-model training, and operative training. Five trainees with different experiences in open pyeloplasty and laparoscopy were exposed to the program. The mentor performed objective and subjective evaluations at each stage to ensure the training quality and operation safety. The perioperative parameters of five... Show More
- Abstract: The expression of fibulin-5 was investigated in urothelial carcinomas of bladder and in normal bladder samples. The effects of fibulin-5 on cell proliferation, motility, and invasion were further explored in bladder cancer cell line 5637. The expression of fibulin-5 in 20 bladder carcinoma samples and 7 normal bladder samples were detected by Western blot. Fibuin-5 cDNA was cloned into p-EGFP-N1 vector and transfected into bladder cancer cell line 5637. Growth curves were estimated by the... Show More
- Abstract: To develop a new porcine model with horn of the uterus to mimic an enlarged ureter for training for laparoscopic ureteral reimplantation (LUR) and to evaluate its feasibility. Ten female pigs were used in the training. The pig was placed to a dorsal position after an anesthetic was administered. The horn of the uterus near the bladder was dissected, then spatulated and trimmed to replace the enlarged ureter. LUR was performed according to standard operation steps. Four trainees completed the... Show More
- Abstract: To study the effect on promoter de-methylation, expression of ALDH1a2 gene and cell apoptosis by treated with 5-Aza-dC and TSA in five human bladder cancer cell lines. Human bladder cancer cell lines RT-4, 253J, 5637, BIU-87 and T24 were cultured and treated with 5-Aza-dC and(or) TSA. The expression of the ALDH1a2 gene was detected by RT-PCR and Western blot. The methylation status of gene promoter was determined by MSP, and the cell cycle profile was established by flow cytometry. ALDH1a2... Show More
- Abstract: To present our surgical techniques and experience with retroperitoneal laparoscopic ureteroureterostomy in 10 patients with retrocaval ureter and review the data on the laparoscopic management of retrocaval ureter published in English. A total of 10 patients with retrocaval ureter underwent laparoscopic ureteroureterostomy. A 3-port, finger and balloon-dissecting, retroperitoneal approach was used. The retrocaval segment of ureter was mobilized and transposed anterior to the inferior vena... Show More
- Abstract: To our knowledge we present the initial experience with and the short-term outcome of laparo-endoscopic single site anatomical retroperitoneoscopic adrenalectomy using conventional instruments. Between June 2009 and April 2010, 25 patients underwent laparo-endoscopic single site anatomical retroperitoneoscopic adrenalectomy. A TriPort™ Access System was inserted through a 2.5 to 3.0 cm transverse skin incision below the tip of the 12th rib. Adrenalectomy was done using a 5 mm 30-degree... Show More
- Abstract: The indication for laparoscopic total or partial adrenalectomy in patients with aldosterone producing adrenal adenoma remains controversial. We compared retroperitoneoscopic partial and total adrenalectomy for aldosterone producing adrenal adenoma in a prospective, randomized, multicenter trial. Patients with aldosterone producing adrenal adenoma were randomized to retroperitoneoscopic partial or total adrenalectomy. Patient characteristics, surgical data, complications and postoperative... Show More
- Abstract: To present our surgical techniques and experience with retroperitoneal laparoscopic upper pole nephroureterectomy for a duplex kidney in adult patients without vesicoureteral reflux. A total of 32 adult patients with a duplex kidney underwent laparoscopic upper pole nephroureterectomy. A 3-port, finger- and balloon-dissecting, retroperitoneal approach was used. The upper pole renal parenchyma was divided circumferentially between the upper and lower poles using a harmonic scalpel,... Show More
- Abstract: Laparoscopic adrenalectomy has become the gold-standard for the surgical treatment of most adrenal lesions. This study evaluated the operative outcome of laparoendoscopic single-site (LESS) retroperitoneoscopic adrenalectomy (LESS-ARA) in comparison with the current standard operation procedure. Between June and December 2009, 19 patients underwent LESS-ARA, and their outcomes were compared with a contemporary 1:2 matched-pair cohort of 38 patients who underwent standard ARA by the same... Show More
- Abstract: Objective To explore the correlation between microRNA-34a (miR-34a) and Notch1, and evaluate the influence of miR-34a overexpression on proliferation of bladder cancer cell line T24. Methods Bioinformatics software were used for predicting the binding site of miR-34a and Notch1, and luciferase assay was performed for confirming the direct regulatory relationship between them. miR-34a plasmid was transfected into bladder cancer cell line T24. Notch1 expression was detected by quantitative... Show More
- Abstract: Purpose: The purpose of this study was to introduce a new method for locating the renal artery during retroperitoneal laparoscopic renal surgery. Patients and methods: The medial arcuate ligament (MAL) is a tendinous arch in the fascia under the diaphragm that arches across the psoas major muscle and is attached medially to the side of the first or the second lumbar vertebra. The renal artery arises at the level of the intervertebral disc between the L1 and L2 vertebrae. We evaluate the... Show More
- Abstract: The oncological and functional results of 329 cases in a population treated with extraperitoneal laparoscopic radical prostatectomy (ELRP) were evaluated retrospectively. A total of 329 inconsecutive patients with prostate cancer (PCa) who underwent ELRP were retrospectively analyzed. The median initial prostate-specific antigen (PSA) level was 17.35 ng/ml. The median biopsy Gleason score was 7.77. Patients with a T2 or T3a clinical stage had received preoperative neoadjuvant hormonal... Show More
- Abstract: The aim of this study was to validate the advantages of the intrafascial nerve-sparing technique compared with the interfascial nerve-sparing technique in extraperitoneal laparoscopic radical prostatectomy. From March 2010 to August 2011, 65 patients with localized prostate cancer (PCa) underwent bilateral intrafascial nerve-sparing extraperitoneal laparoscopic radical prostatectomy. These patients were matched in a 2∶1 ratio to 130 patients with localized PCa who had undergone bilateral... Show More
- Abstract: Partial nephrectomy is currently the standard treatment for clinical T1 renal neoplasms, as it can provide oncologic outcomes equivalent to radical nephrectomy. The aim was to evaluate the efficacy of self-retaining suture (SRS) in renorrhaphy technique in retroperitoneal laparoscopic partial nephrectomy (LPN) for a single renal mass of moderate or high complexity by assessing peri-operative outcomes. A retrospective analysis was done of 64 patients between 2010 and 2012 for complex renal... Show More
Publications citing this author (229)
[Show abstract] [Hide abstract] ABSTRACT: This study aims firstly to assess the most adequate surgical approach for the creation of an ureteropelvic juntion obstruction (UPJO) animal model, and secondly to validate this model for laparoscopic pyeloplasty training among urologists. Thirty six Large White pigs (28.29±5.48 Kg) were used. The left ureteropelvic junction was occluded by means of an endoclip. According to the surgical approach for model creation, pigs were randomized into: laparoscopic conventional surgery (LAP) or single port surgery (LSP). Each group was further divided into transperitoneal (+T) or retroperitoneal (+R) approach. Time needed for access, surgical field preparation, wound closure, and total surgical times were registered. Social behavior, tenderness to the touch and wound inflammation were evaluated in the early postoperative period. After ten days, all animals underwent an Anderson-Hynes pyeloplasty carried out by 9 urologists, who subsequently assessed the model by means of a subjective validation questionnaire. Total operative time was significantly greater in LSP+R (p=0.001). Tenderness to the touch was significantly increased in both retroperitoneal approaches, (p=0.0001). Surgeons rated the UPJO porcine model for training on laparoscopic pyeloplasty with high or very high scores, all above 4 on a 1-5 point Likert scale. Our UPJO animal model is useful for laparoscopic pyeloplasty training. The model created by retroperitoneal single port approach presented the best score in the subjective evaluation, whereas, as a whole, transabdominal laparoscopic approach was preferred.
- As far as we know, UPJO swine model has not been used for training. Zhang et al  reported the use of a healthy porcine animal model for laparoscopic pyeloplasty training by using a small intestine segment to simulate the enlarged renal pelvis. In our opinion, this model is perfect for enhancing intracorporeal suturing skills, whereas it is not as suitable as ours for dissection maneuvers, as it lacks fibrosis, adhesions, etc.
[Show abstract] [Hide abstract] ABSTRACT: Introduction: The safety and efficacy of laparoscopic transperitoneal lateral adrenalec-tomy and retroperitoneoscopic adrenalectomy have been reported. The aim is to report the authors' experience in laparoscopic left adrenalectomy with an alternative trans-peritoneal submesocolic and retropancreatic approach with patient supine. Research methods: The authors have performed laparoscopic transperitoneal submesocolic, retropancreatic adrenalectomy for both benign and malignant, functioning lesions >4cm in diameter or with smaller lesions but having an imaging pattern suspicious of malig-nancy or of sub-clinically secreting tumors. After opening the posterior peritoneum at the root of the transverse mesocolon and Gerota's fascia, the junction of the inferior adrenal vein with the left renal vein is identified. The adrenal vein is then prepared and divided, followed by mobilization and removal of the left adrenal gland. Conclusion: Early ligation of the adrenal vein is the most relevant technical feature of this procedure to avoid the release of catecholamines, hormones or neoplastic cells which could occur during manipulation of the gland prior to ligation of the main adrenal vein. Moreover, this approach makes it possible to perform associated procedures , including a bilateral adrenalectomy, without the need to reposition the patient on the operative table, but simply by positioning additional trocars.
- This initial experience has been followed by others using the same approach or, alternatively , the retroperitoneal approach with the patient in the prone or in the lateral decubitus position [6, 7] and the transperitoneal approach with the patient supine . Several reports have confirmed the safety and efficacy of these techniques, and minimally, invasive adrenalectomy is presently considered the treatment of choice for a variety of benign lesions, including lesions of the medulla [9, 10]. The aim of the present chapter is to report the authors' experience with left laparoscopic adrenalectomy (LA) using an original transperitoneal submesocolic and retropancreatic approach with patient supine, to describe the patient's preparation for surgery as well as the operative details of this procedure.
[Show abstract] [Hide abstract] ABSTRACT: Single nucleotide polymorphisms (SNPs) occurring in noncoding sequences have largely been ignored in genome-wide association studies (GWAS). Yet, amounting evidence suggests that many noncoding SNPs especially those that are in the vicinity of protein coding genes play important roles in shaping chromatin structure and regulate gene expression and, as such, are implicated in a wide variety of diseases. One of such regulatory SNPs (rSNPs) is the E-cadherin (CDH1) promoter -160C/A SNP (rs16260) which is known to affect E-cadherin promoter transcription by displacing transcription factor binding and has been extensively scrutinized for its association with several diseases especially malignancies. Findings from studying this SNP highlight important clinical relevance of rSNPs and justify their inclusion in future GWAS to identify novel disease causing SNPs.
- but not in black and white Americans . Three case-control studies have observed that the A allele of E-cadherin C/A SNP confers higher risk for bladder cancer in the Chinese , Japanese , and Dutch  and is associated with invasive cancer . Of particular note is a clinical outcome study following 302 patients with superficial bladder cancer after transurethral resection of the tumors for a median follow-up of 27.65 months .
[Show abstract] [Hide abstract] ABSTRACT: Objective To comprehensively review current reports on the complications of laparoendoscopic single-site surgery (LESS), introduced recently into urology as an option for treating various urological pathologies.Methods We reviewed previous reports to August 2011 using Medline, focusing on LESS in urology, with special interest in the complications, evaluating those during and after surgery, as well as conversions to reduced-port laparoscopy, conventional laparoscopy and open surgery.ResultsThere are increasing reports of LESS in urology, with expanding indications. Complication rates both during and after surgery are low and related mostly to the technical difficulty and dexterity with the currently available instruments. Overall, intraoperative complications were reported by 11 published studies, while postoperative complications were reported by 15. Although the overall conversion rates to open surgery and conventional laparoscopy were low, the incidence of reduced-port laparoscopy was significantly higher.Conclusions Although there are expanding indications for LESS in urology, the risk of complications is low. This might be related to the fact that LESS is still restricted to experienced laparoscopic surgeons, and to the criteria for selecting patients.
- There was no analysis of risk factors for conversions. Other smaller series reported complications in two patients (postoperative angina and contralateral atelectasis) of 19 patients who underwent LESS adrenalectomy .
[Show abstract] [Hide abstract] ABSTRACT: The incidence of prostate cancer (PCa) within Asian population used to be much lower than in the Western population; however, in recent years the incidence and mortality rate of PCa in some Asian countries have grown rapidly. This collaborative report summarized the latest epidemiology information, risk factors, and racial differences in PCa diagnosis, current status and new trends in surgery management and novel agents for castration-resistant prostate cancer. We believe such information would be helpful in clinical decision making for urologists and oncologists, health-care ministries and medical researchers.
- For instance, Single-port LRP was preferred by Wen et al.  and has been shown to achieve better results in selected patients. Extraperitoneal LRP was preferred by Zheng et al.  for its advantages in preventing intra-abdominal complications and combined the advantages of minimally invasive laparoscopy and retropubic open prostatectomy. There are now several high-volume centers for RP with a well-designed follow-up system that makes the long-term assessment of the surgery result possible in developed countries such as Japan, Korea, Israel and developing countries such as China and India.
[Show abstract] [Hide abstract] ABSTRACT: Treatment of primary aldosteronism (PA) aims at preventing or correcting hypertension, hypokalemia and target organ damage. Patients with lateralized PA and candidates for surgery may be managed by laparoscopic adrenalectomy. Partial adrenalectomy and non-surgical ablation have no proven advantage over total adrenalectomy. Intraoperative morbidity and mortality are low in reference centers, and day-surgery is warranted in selected cases. Spironolactone administered during the weeks preceding surgery controls hypertension and hypokalemia and may prevent postoperative hypoaldosteronism. In most cases, surgery corrects hypokalemia, improves control of hypertension and reduces the burden of pharmacologic treatment; in about 40% of cases, it resolves hypertension. However, success in controlling hypertension and reversing target organ damage is comparable with mineralocorticoid receptor antagonists. Informed patient preference with regard to surgery is thus an important factor in therapeutic decision-making.
- Adenomectomy is possible and entails no extra complications compared to total adrenalectomy [8,9] . Operative times are similar , although intraoperative blood loss is significantly greater in adenectomy, without, however, requiring supplementary trans- fusion [8,9]. The rationale supporting conservative surgery, however, is unclear in lateralized PA, which never shows recurrence in the contralateral adrenal; moreover, 10–25% of patients have several adjacent nodules, whether or not visible on preoperative imaging, and there is no way of knowing which is responsible for the PA, entailing a risk of incomplete treatment if part of the adrenal is spared .
[Show abstract] [Hide abstract] ABSTRACT: Fibulin-5 has recently been considered as a potential tumor suppressor in human cancers. Several studies have shown that it is down-regulated in a variety of tumor types and inhibits tumor growth and metastasis. This study was aimed to investigate the clinical significance of fibulin-5 in glioma and its role in cell proliferation and invasion. We found that the expression of fibulin-5 in glioma tissues was significantly lower than those in normal brain (NB) tissues. Negative expression was significantly correlated with advanced clinical stage (grade III+IV) . Furthermore, Fibulin-5 negative expression was correlated with a shorter overall survival of glioma patients. Multivariate Cox repression analysis indicated that fibulin-5 was an independent factor for predicting overall survival of glioma patients. Overexpression obviously inhibited cell proliferation in U251 and U87 cells. Furthermore, it significantly reduced the number of migrating and invading glioma cells. In conclusion, impaired expression of fibulin-5 is correlated with the advanced tumor stage in glioma. Otherwise, Fibulin-5 is an independent prognostic marker for predicting overall survival of glioma patients. Mechanistically, it may function as a tumor suppressor via inhibiting cell proliferation and invasion in gliomas.
- The ECM protein Fibulin-5, a transforming growth factor (TGF)-β-induced glycoprotein, is a multifunctional molecule that regulates various cellular processes, including proliferation, motility and invasion, in normal and malignant cells (Hu et al., 2011;Li et al., 2012;Xiao et al., 2013). Reduced expression of Fibulin-5 has been observed in a number of human cancers (Schiemann et al., 2002;Wlazlinski et al., 2007;Yanagisawa et al., 2009;Hu et al., 2011;Ohara et al., 2011). In this study, the expression of Fibulin-5 was detected in 86 glioma tissues and 20 NB tissues using immunohistochemical staining.
[Show abstract] [Hide abstract] ABSTRACT: Background: Notch signalling regulates cell fate in most tissues, promoting precursor cell proliferation in some, but differentiation in others. Accordingly, downregulation or overactivity variously contributes to cancer development. So far, little is known about Notch pathway activity and function in the normal urothelium and in urothelial carcinoma (UC). We have therefore investigated expression of Notch pathway components in UC tissues and cell lines and studied the function of one receptor, NOTCH1, in detail. Methods: Expression of canonical Notch pathway components were studied in UC and normal bladder tissues by immunohistochemistry and quantitative RT-PCR and in UC cell lines and normal cultured urothelial cells by qRT-PCR, immunocytochemistry and Western blotting. Pathway activity was measured by reporter gene assays. Its influence on cell proliferation was investigated by γ-secretase inhibition. Effects of NOTCH1 restoration were followed by measuring cell cycle distribution, proliferation, clonogenicity and nuclear morphology. Results: NOTCH1 and its ligand, DLL1, were expressed at plasma membranes and in the cytoplasm of cells in the upper normal urothelium layer, but became downregulated in UC tissues, especially in high-stage tumours. In addition, the proteins were often delocalized intracellularly. According differences were observed in UC cell lines compared to normal urothelial cells. Canonical Notch pathway activity in reporter assays was repressed in UC cell lines compared to normal cells and a mammary carcinoma cell line, but was induced by transfected NOTCH1. Inhibitors of Notch signalling acting at the γ-secretase step did not affect UC cell proliferation at concentrations efficacious against a cell line with known Notch activity. Surprisingly, overexpression of NOTCH1 into UC cell lines did not significantly affect short-term cell proliferation, but induced nuclear abnormalities and diminished clonogenicity. Conclusion: Our data indicate that canonical Notch signalling is suppressed in urothelial carcinoma mainly through downregulation of NOTCH1. These findings can be explained by proposing that canonical Notch signalling may promote differentiation in the urothelium, like in many squamous epithelia, and its suppression may therefore be advantageous for tumour progression. As an important corollary, inhibition of canonical Notch signalling is unlikely to be efficacious and might be counter-productive in the treatment of urothelial carcinoma.
- NOTCH1 and DLL1 were most consistently diminished in both cell lines and tissues. Downregulation of these factors is in accord with a previous report , whereas another paper reported decreased NOTCH1 expression in high-grade invasive UC tissues only, with more pervasive cytoplasmic localization . The mechanisms underlying these changes remain to be determined.
[Show abstract] [Hide abstract] ABSTRACT: The article explains the scientific reasons for the diaphragm muscle being an important crossroads for information involving the entire body. The diaphragm muscle extends from the trigeminal system to the pelvic floor, passing from the thoracic diaphragm to the floor of the mouth. Like many structures in the human body, the diaphragm muscle has more than one function, and has links throughout the body, and provides the network necessary for breathing. To assess and treat this muscle effectively, it is necessary to be aware of its anatomic, fascial, and neurologic complexity in the control of breathing. The patient is never a symptom localized, but a system that adapts to a corporeal dysfunction.
- Their tendons are shaped as a tendinous arc in front of T12 (median arcuate ligament), and is crossed by the aortic artery and thoracic duct.8,12 The lateral pillars insert here and split into two robust tendons: one, the medial arcuate ligament (above the psoas muscle) bridging the L1 vertebra and the transverse apophysis,13 and the other, the lateral arcuate ligament (above the quadratus lumborum muscle) connecting the transverse process of L1 and the apex of the twelfth rib.14 The medial and lateral arcuate ligaments act as a bridge between the thoracolumbar fascia posteriorly and the transversalis fascia anteriorly.15–17
[Show abstract] [Hide abstract] ABSTRACT: Laparo-endoscopic single-site adrenalectomy (LESS-A) is commonly performed using specialized access devices and/or instruments. We report a LESS-A in a 47-year-old woman with a left aldosteranoma via a subcostal approach utilizing conventional laparoscopic ports and instruments. The feasibility and cost-effectiveness of this approach are highlighted and the literature on the subject is reviewed.
- Both retroperitoneoscopic (R‑LESS‑A) and transperitoneal (T‑LESS‑A) approaches to LESS‑A have been described, and different variations in the technique are practiced for each. Zhang et al. attempted 25 R‑LESS‑A using a TriPort (Advanced Surgical Concepts, Bray, Ireland) through a 2.5‑3 cm incision and two conventional instruments.  An additional 5 mm port was required in one patient and one patient required a conversion to LA. They noticed a reduction in the mean operative time from 62 minutes in the first 10 cases to 52 minutes in the subsequent 15. Chung et al. reported R‑LESS‑A in 7 patients using a glove‑port made with an Alexis wound retractor combined with s
[Show abstract] [Hide abstract] ABSTRACT: . Retrocaval ureter is a rare congenital anomaly. Open surgery was the classic treatment for this condition. Laparoscopy is currently an admitted procedure to treat many urological diseases. The objective of our study is to present our experience and discuss the safety and the feasibility of transperitoneal laparoscopic pyelopyelostomy for treatment of retrocaval ureter (RCU). Materials and Methods . Three symptomatic patients underwent laparoscopic repair for RCU in our department. The diagnosis was suspected on the computed tomography scan (CT) and confirmed on ascending pyelography. After placement of a JJ stent, and, using the transperitoneal approach, the retro peritoneum was exposed; the ureter was identified in both sides of the vena cava. The retrocaval segment was entirely mobilized and pulled from behind of the vena cava after section of renal pelvis. A pyelopyelostomy was done in a normal anatomic position. Results . All operations were achieved laparoscopically without conversion to open surgery. The mean operative time was 140 minutes (110–190). No intraoperative complication occurred. Blood loss was less than 50 mL in all patients. The mean hospital stay was 5 days (4–6 days). All patients were symptom-free after surgery and had reduction of hydronephrosis in control imagery. Conclusion . Laparoscopy seems safe, feasible, and reproducible in managing retrocaval ureter.
- The mean blood loss was 20 mL (range 15–50). Li et al.  operated on a total of 10 patients. All operations were completed laparoscopically.