Ailing Sun

Liaocheng Teachers University, Tungchangfu, Shandong Sheng, China

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Publications (46)119.77 Total impact

  • Chunpeng Diao · Cong Li · Xiao Yang · Ailing Sun · Renmin Liu
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    ABSTRACT: Magnetic matrix solid phase dispersion (MMSPD) assisted dispersive liquid liquid microextraction (DLLME) was applied to extract ultra traces of polychlorinated biphenyls (PCBs) from water samples prior to gas chromatography with electron capture detection. PCBs in water were adsorbed by micro particles of magnetic bamboo charcoal and then transferred into the elution solvent. PCBs in the elution solvent of the MMSPD were further concentrated into trace volume extraction solvent of the DLLME procedure. Under optimized conditions, good linearity in the range of 0.2–100 ng L−1 was obtained with regression coefficients (r) higher than 0.9987. Based on a signal-noise ratio of 3, the limits of detection (LODs) range from 0.05–0.1 ng L−1. These LODs are much lower than those of MMSPD or DLLME alone. Relative standard deviations are between 4.9–8.2 %. The method was successfully applied to the determination of PCBs in lake and river water. Relative recoveries were 85.5–117.4 % for the spiked environmental water samples. Graphical Abstract Polychlorinated biphenyls (PCBs) were adsorbed by micro particles of magnetic bamboo charcoal (MBC) and then eluted. PCBs were further concentrated into a trace volume of the extraction solvent used in dispersive liquid liquid microextraction.
    No preview · Article · Jan 2016 · Microchimica Acta
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    ABSTRACT: In this study, a preparative separation method was developed for isolation of eleven polyphenols from water-soluble fraction of Chinese propolis using macroporous absorptive resin (MAR) coupled with preparative high performance liquid chromatography (PHPLC). Water-soluble fraction of Chinese propolis was first “prefractioned” using MAR, which yielded four subfractions. The four subfractions were then isolated by PHPLC with an isocratic elution of methanol-water. Finally, eleven polyphenols were purified from Chinese propolis including caffeic acid, ferulic acid, isoferulic acid, 3, 4-dimethoxy cinnamic acid, pinobanksin, caffeic acid benzyl ester, caffeic acid phenethyl ester, apigenin, pinocembrin, chrysin and galangin. The purities of the compounds were determined by HPLC and the chemical structures were confirmed by UV and NMR analysis. The method developed was simple, effective, rapid, scalable and economical, and it was a promising basis for large-scale preparation of multiple components from natural products.
    No preview · Article · Jan 2016 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • Chunpeng Diao · Xiao Yang · Ailing Sun · Renmin Liu
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    ABSTRACT: Magnetic matrix solid phase dispersion (MMSPD) assisted dispersive liquid-liquid microextraction (DLLME) for the ultrasensitive determination of bisphenol A (BPA) in environmental water was developed with high performance liquid chromatography-fluorescence (HPLC-FLU) detection. Microparticles of magnetic activated carbon (MAC) were synthesized and used to conduct the MMSPD procedure. The specific surface area of the MAC was 287 m2 g-1, and the saturation magnetization of the MAC supported by Fe3O4 nanoparticles was up to 20.7 emu g-1. BPA in water was adsorbed by microparticles of MAC and then transferred into the elution solvent by elution. The DLLME procedure was assisted by the elution solvent acting as the dispersive solvent. BPA in the elute solvent of the MMSPD procedure was concentrated into the trace volume extraction solvent of the DLLME procedure. A sufficiently low limit of detection (LOD) of 0.002 μg L-1 for the determination of BPA in environmental water was obtained. Good linearity was obtained throughout the concentration range of 0.006 to 5.0 μg L-1 with a correlation coefficient (R2) of 0.9991. The relative standard deviation (RSD) was 6.2%. The relative recoveries ranged from 86.6 to 106.3% for the spiked real water samples. The results showed that the MMSPD assisted DLLME technique is highly sensitive and reliable for the determination of trace BPA in environmental water.
    No preview · Article · Dec 2015 · Analytical methods
  • Xiaoyuan Ding · Yan Tang · Ailing Sun · Renmin Liu
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    ABSTRACT: A reversed-phase high-performance liquid chromatography method for the simultaneous determination of jatrorrhizine, palmatine and berberine in Huangbo, the dried bark of Chinese Corktree, was established by using 1-hexyl-3-methylimidazolium tertafluoroborate as a mobile phase additive. The chromatographic behavior of the three compounds on the C18 column was studied with four different types of 1-alkyl-3-methylimidazolium-based ionic liquids as the mobile phase additives. The effect of 1-hexyl-3-methylimidazolium tertafluoroborate was the best in the four investigated ionic liquids. The concentration of 1-hexyl-3-methylimidazolium tertafluoroborate and the pH of the mobile phase, which influenced the chromatographic behaviors of the three bioactive compounds, were investigated. The linearity, precision, accuracy, repeatability, limit of detection and quantification of the proposed method were found to be satisfactory. To explain the role of ionic liquids as the mobile phase additives, the possible mechanism was also explored and discussed.This article is protected by copyright. All rights reserved
    No preview · Article · Nov 2014 · Journal of Separation Science
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    ABSTRACT: In this study, a simple and efficient preparative procedure was developed for preparation of seven flavonoids from the peel of Trichosanthes kirilowii Maxim. using polyamide resin followed by semi-preparative high performance liquid chromatography (SPHPLC). First, the ethyl acetate fraction from the peel of T. kirilowii Maxim. obtained “prefractionation” using polyamide resin, which yielded two subfractions. And then the two subfractions were isolated by SPHPLC with an isocratic elution of methanol–water. Finally, seven known flavonoids were purified from 35 g of ethyl acetate extract including quercetin-3-O-[α-l-rhamnose (1→2)-β-d-glucopyranosyl]-5-O-β-d-glucopyranoside (19 mg), quercetin-3-O-rutinoside (24 mg), apigenin-7-O-β-d-glucopyranoside (10 mg), diosmetin-7-O-β-d-glucopyranoside (45 mg), luteolin (21 mg), apigenin (15 mg), and diosmetin (56 mg). The purities of the compounds were determined by HPLC and the chemical structures were confirmed by UV and NMR analysis. In the present study, a simple, effective, and rapid procedure was established for preparative separation of multiple components from the peel of T. kirilowii Maxim. Furthermore, it was scalable and economical, so it was a promising basis for large-scale preparation of flavonoids from other plant extracts.
    No preview · Article · Aug 2014 · Journal of Chromatography B
  • Rui Wang · Xiuhuan Zhu · Ailing Sun · Renmin Liu
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    ABSTRACT: A preparative high speed counter-current chromatography (HSCCC) method for isolation and purification of flavonoid compounds from SCUTELLARIAE RADIX, the roots of Chinese medicinal plant Scutellaria baicalensis Georgi, was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Baicalin and wogonoside were purified from the crude extract of SCUTELLARIAE RADIX by using ethyl acetate-water-[C4mim][PF6] (5:5:0.2, v/v) as two-phase solvent system. From 120 mg of the crude sample, 50.1 mg of baicalin and 45.6 mg of wogonoside were obtained in a one step separation. The purities of baicalin and wogonoside were 99.3% and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by1H-NMR and 13C-NMR.
    No preview · Article · Apr 2014 · Journal of Liquid Chromatography & Related Technologies
  • Minglan Yu · Ailing Sun · Yongqing Zhang · Renmin Liu
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    ABSTRACT: In this paper, a chromatographic method for isolation and purification of coumarin compounds from Cortex fraxinus was established by using Superose 12 as the separation media for the first time. The conditions for separation were optimized. Four kinds of coumarin compounds including aesuletin, aesculin, fraxetin and fraxin were obtained. The purity of these compounds were 98.5, 99.1, 97.9 and 97.3%, respectively, which were determined by HPLC area normalization method. The chemical structures of the separated compounds were identified according to 1H and 13C nuclear magnetic resonance data. The retention behavior of the separated coumarin compounds on Superose 12 was also discussed. The retention is based on a mixture of hydrogen bonding and hydrophobic interactions between the coumarin compounds and the residues of the cross-linking reagents used in the manufacturing process of Superose 12. The results of this paper indicate that Superose 12 is not only suitable for size-exclusion chromatography of proteins and other biological macromolecules but also for low-molecular-weight natural products.
    No preview · Article · Oct 2013 · Journal of chromatographic science
  • Jing Wang · Ailing Sun · Renmin Liu · Yongqing Zhang
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    ABSTRACT: A chromatographic method for isolation and purification of soy isoflavones from soybean extracts was established by using 12% cross-linked agarose gel, Superose 12, as the separation media. 30% and 65% methanol in gradient elution mode was employed for separation. Soybean extracts were separated by the proposed method and six soy isoflavones, including glycitin, daidzin, genistin, glycitein, daidzein, and genistein, were obtained. The purity of these compounds was 97.8%, 98.5%, 99.4%, 99.0%, 98.8%, 99.2%, respectively, which was determined by HPLC area normalization method. The retention behavior of soy isoflavones on Superose 12 was also discussed. The retention is based on a mixture of hydrogen bonding and hydrophobic interactions between the hydroxyl groups of soy isoflavones and the residues of the cross-linking reagents used in the manufacturing process of Superose 12.
    No preview · Article · Jun 2013 · Journal of Liquid Chromatography & Related Technologies
  • Jing Liu · Renmin Liu · Ailing Sun · Yongqing Zhang
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    ABSTRACT: High-speed counter-current chromatography (HSCCC) and evaporative light scattering detector (ELSD) were connected together by flow injection for the first time. A new method for isolation and purification of ginkgolide compounds from GINKGO BILOBA L. was developed. Two pairs of two-phase solvent system, n-hexane-ethyl acetate-methanol-water (4:5:1:5, v/v) and n-hexane-ethyl acetate-methanol-water (4:5:2:5, v/v), were used in HSCCC separation. The stationary phase was the upper phase of n-hexane-ethyl acetate-methanol-water (4:5:1:5, v/v). The mobile phase was the lower phase of n-hexane-ethyl acetate-methanol-water (4:5:1:5, v/v) and n-hexane-ethyl acetate-methanol-water (4:5:2:5, v/v) in gradient elution mode. GINKGO BILOBA L. powders were degreased by light petroleum and extracted with 70% ethanol. The extracts were purified with ethyl acetate extraction and acidic alumina oxide column separation, and 2.4 g of purified extracts were obtained from 500 g of GINKGO BILOBA L. 107 mg of ginkgolides A, 64 mg of ginkgolides B, 52 mg of ginkgolides C, and 83 mg of bilobalide were obtained in one-step HSCCC separation from 500 mg of the purified extracts. The purities of the obtained compounds were all above 98% as determined by HPLC-ELSD area normalization method and their chemical structures were identified by 1H-NMR and 13C-NMR.
    No preview · Article · Jun 2013 · Journal of Liquid Chromatography & Related Technologies
  • Yan Tang · Ailing Sun · Renmin Liu · Yongqing Zhang
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    ABSTRACT: A reversed phase high performance liquid chromatography (RP-HPLC) method for simultaneous determination of fangchinoline (FAN) and tetrandrine (TET) in Stephania tetrandra S. Moore was established by using 1-hexyl-3-methylimidazolium tetrafluoroborate as the mobile phase additives in this paper. Four types of 1-alkyl-3-methylimidazolium-based ionic liquids (ILs) were used as additives of the mobile phase to separate FAN and TET by RP-HPLC. The effects of the length of the alkyl group on the imidazolium ring and its counterion, the concentrations of IL and the pH of the mobile phase, which influenced the chromatographic behaviors of FAN and TET, were investigated in detail. The linearity, sensitivity, accuracy and repeatability of the proposed method were also investigated. The probable mechanism of the separation with ILs as the mobile phase additives was explored and discussed.
    No preview · Article · Mar 2013 · Analytica chimica acta
  • Aifeng Li · Ailing Sun · Renmin Liu · Yongqing Zhang
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    ABSTRACT: High-speed counter-current chromatography was applied in the isolation and purification of four isoflavonoids from the Chinese medicinal plant Belamcanda chinensis(L.) DC. Preparative high-speed counter-current chromatography method with two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at volume ratio of 5:5:3:7 was successfully established, which yielded 6.2 mg of tectoridin, 8.5 mg of iridin, 22.6 mg of irigenin and 13.4 mg of irisflorentin from 150 mg of the crude extract in one-step separation. The purities were 98.1%, 98.9%, 99.0% and 99.2%, respectively, as determined by high performance liquid chromatography and their chemical structures were identified by H-NMR and C-NMR.
    No preview · Article · Dec 2012 · Journal of Liquid Chromatography & Related Technologies
  • Xia Li · Ailing Sun · Renmin Liu · Yongqing Zhang
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    ABSTRACT: A preparative high-speed counter-current chromatography (HSCCC) method for separation and purification of biondnoid I and magnolin from the Chinese medicinal plant FLOS MAGNOLIAE was successfully established by using light petroleum-ethyl acetate-methanol-water (3:7:4:6, v/v) as the two-phase solvent system. From 500 mg of crude extracts of FLOS MAGNOLIAE 48 mg of biondnoid I with the purity of 99.3% and 135 mg of magnolin with the purity of 98.7% were separated in a one-step separation. The structures of biondnoid I and magnolin were identified by H-NMR.
    No preview · Article · Oct 2012 · Journal of Liquid Chromatography & Related Technologies
  • Aifeng Li · Yongqing Zhang · Ailing Sun · Renmin Liu
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    ABSTRACT: A new high speed counter-current chromatography (HSCCC) method for preparative separation and purification of three phenolic acids from the dried buds of Lonicera japonica Thunb was developed by using gradient elution mode. Two pairs of two-phase solvent system composed of n-hexane-ethyl acetate-methanol-0.01 M hydrochloric acid at volume ratios of 1:5:1:5 and 1:5:2:5 were used in HSCCC separation. The upper phase of n-hexane-ethyl acetate-methanol-0.01 M hydrochloric acid (1:5:1:5, v/v) was used as the stationary phase. The lower phase of n-hexane-ethyl acetate-methanol-0.01 M hydrochloric acid (1:5:1:5, v/v) and (1:5:2:5, v/v) were used as the mobile phase in gradient elution mode. From 200 mg of the crude extract 51.6 mg of 3-caffeoylquinic acid, 7.5 mg of caffeic acid, and 42.4 mg of 3,5-O-dicaffeoylquinic acid were obtained in one-step HSCCC separation. The purities of the obtained compounds were all above 95% as determined by HPLC area normalization method, and their chemical structures were identified by H-NMR and C-NMR.
    No preview · Article · Aug 2012 · Journal of Liquid Chromatography & Related Technologies
  • Wenwen Dong · Ailing Sun · Yongqing Zhang · Renmin Liu
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    ABSTRACT: A chromatographic method for isolation and purification of active components from Polygonum cuspidatum Sieb. et Zucc was established by using Superose 12 as the separation media. 65% methanol was used as the mobile phase for separation. Four components including piceid, resveratrol, anthraglycoside B and emodin were obtained in one-step separation. The purity of these compounds was 98.3%, 99.3%, 98.7%, 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of these compounds were identified according to their H NMR and C NMR data.
    No preview · Article · Jan 2012 · Journal of Liquid Chromatography & Related Technologies
  • Aifeng Li · Ailing Sun · Renmin Liu
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    ABSTRACT: High-speed counter-current chromatography was applied in the isolation and purification of three flavonoids from the Chinese medicinal plant Alpinia katsumadai Hayata. Two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at volume ratios of 5:5:7:3 was used, which yielded 17.2 mg of alpinetin, 3.5 mg of pinocembrin, and 25.1 mg of cardamomin from 100 mg of the crude extract in one-step separation. The purities of them were 98.1%, 97.5% and 99.2% as determined by high performance liquid chromatography and their chemical structures were identified by H-NMR and C-NMR.
    No preview · Article · Jan 2012 · Journal of Liquid Chromatography & Related Technologies
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    ABSTRACT: A method for extraction and preparative separation of tanshinones from Salvia miltiorrhiza Bunge was successfully established in this paper. Tanshinones from Salvia miltiorrhiza Bunge were extracted using ethyl acetate as the extractant under reflux. The extracts were then purified by high speed counter-current chromatography (HSCCC) with light petroleum-ethyl acetate-methanol-water (6:4:6.5:3.5, v/v) as the two phase solvent system. The upper phase was used as the stationary phase and the lower phase as the mobile phase. 8.2mg of dihydrotanshinone I, 5.8 mg of 1,2,15,16-tetrahydrotanshiquinone, 26.3mg of cryptotanshinone, 16.2mg of tanshinone I, 25.6 mg of neo-przewaquinone A, 68.8 mg of tanshinone IIA and 9.3mg of miltirone were obtained from 400mg of extracts from Salvia miltiorrhiza Bunge in one-step HSCCC separation, with the purity of 97. 6%, 95.1%, 99.0%, 99.1%, 93.2%, 99.3% and 98.7%, respectively, as determined by HPLC area normalization method. Their chemical structures were identified by ¹H NMR.
    No preview · Article · Jul 2011 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
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    ABSTRACT: A new high-speed counter-current chromatography method for semi-preparative separation and purification of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn was developed by using pH-gradient elution mode. Diethyl ether was used as the stationary phase of the two-phase solvent system and Na(2)HPO(4)/NaH(2)PO(4) buffer solution with pH values of 7.5 and 7.2 in gradient mode as the mobile phase. Consequently, 33 mg of liensinine, 42 mg of isoliensinine, and 67 mg of neferine were obtained from 200 mg of crude extracts. The purities of them were all over 98% as determined by HPLC area normalization method, and the structures were identified by (1)H-NMR and (13)C-NMR.
    Full-text · Article · Jun 2010 · Journal of Separation Science
  • Renmin Liu · Lili Xu · Aifeng Li · Ailing Sun
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    ABSTRACT: A preparative high-speed counter-current chromatography method for isolation and purification of flavonoid compounds from Oroxylum indicum was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Two flavonoid compounds including baicalein-7-O-diglucoside and baicalein-7-O-glucoside were purified from the crude extract of O. indicum by using ethyl acetate-water-[C(4)mim][PF(6)] (5:5:0.2, v/v) as two-phase solvent system. 36.4 mg of baicalein-7-O-diglucoside and 60.5 mg of baicalein-7-O-glucoside were obtained from 120 mg of the crude extract. Their purities were 98.7 and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by (1)H-NMR and (13)C-NMR.
    No preview · Article · Feb 2010 · Journal of Separation Science
  • Lili Xu · Aifeng Li · Ailing Sun · Renmin Liu
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    ABSTRACT: A preparative high-speed countercurrent chromatography method for isolation and purification of neomangiferin and mangiferin from Rhizoma anemarrhenae was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Neomangiferin and mangiferin were purified from the crude extract of R. anemarrhenae by using ethyl acetate-water-[C(4)mim][PF(6)] (5:5:0.2 v/v) as two-phase solvent system. In total, 22.5 mg of neomangiferin and 70.6 mg of mangiferin were obtained from 150 mg of the crude extract. The purities of neomangiferin and mangiferin were 97.2 and 98.1%, respectively, as determined by HPLC. The chemical structures of the isolated compounds were identified by (1)H-NMR and (13)C-NMR.
    No preview · Article · Jan 2010 · Journal of Separation Science
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    Li Meng · Renmin Liu · Ailing Sun · Sujuan Wu · Ningnig Liu
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    ABSTRACT: A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of rutin and acaciin from the Chinese medicinal herb Herba Cirsii was successfully established. The crude extracts obtained from Herba Cirsii by water under reflux were subjected to a macroporous resin column and eluted with 10% and 60% ethanol, respectively. The fraction of 60% ethanol was used as the sample for HSCCC separation of rutin and acaciin. The two-phase solvent system used for the separation was ethyl acetate—n-butanol—water (5:1.5:5, v/v) and the upper phase was used as the stationary phase. Rutin (25.2 mg) and acaciin (21.8 mg) with a purity of 99.2% and 99.6%, respectively, were purified successfully from 60 mg of sample. The chemical structures of rutin and acaciin were identified by 1H-NMR and 13C-NMR.
    Full-text · Article · May 2009 · Journal of chromatographic science