Zivar Salehi

University of Guilan, Resht, Gīlān, Iran

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Publications (70)102.46 Total impact

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    ABSTRACT: Despite enormous progress in the understanding of human reproductive physiology, the underlying cause of male infertility remains undefined in about 50.0% of cases, which are referred to as idiopathic infertility. Human apurinic/apyrimidinic endonuclease 1 (ApE1) is a multifunctional protein that has an important role in the base excision repair pathway. The present study aimed to evaluate whether two functional ApE1 polymorphisms (−656T > G and 1349T > G) are associated with the susceptibility of female infertility. Blood samples were collected from 100 patients diagnosed with female infertility and 100 control subjects and genotyped by tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR). The results indicated that individuals with the variant TG genotypes had a significantly increased risk of female infertility (p = 0.035, OR = 1.98, 95% CI = 1.04–3.74). Whereas, a significant association between 1349T > G polymorphism and female infertility risk was not observed (p = 0.1). Larger studies with more patients and controls are required to confirm the results.
    No preview · Article · Jan 2016 · Journal of Obstetrics and Gynaecology
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    ABSTRACT: Previous studies have suggested that an association exists between the proline dehydrogenase gene (PRODH) and increased schizophrenia risk. We examined the prevalence of the PRODH 757C/T (Arg185Trp), 1766A/G (Gly521Arg), and 1852G/A (intronic mutation) polymorphisms in 175 patients with schizophrenia and 185 control subjects. All subjects were of Iranian ancestry. The PRODH 757TT, 1852AA, and 1766GG genotypes were associated with an increased risk of schizophrenia (odds ratio = 1.38, 95% confidence interval: 0.88-2.16, P = 0.001, P = 0.001, respectively). The activity alleles in the PRODH genotype combinations were associated with an increased risk of schizophrenia (haplotype analysis, TAG genotype P = 0.007). Our findings support a major role for the PRODH 757TT, 1766GG, and 1852AA genotypes alone and in combination for schizophrenia susceptibility.
    Full-text · Article · Oct 2015 · Genetics and molecular research: GMR
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    ABSTRACT: Breast cancer is the most common cancer and one of the main causes of cancer—related death in the world and has become a major public health concern. Many genes have been shown to be involved in the progress of the breast cancer. Human apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that has an important role in the base excision repair (BER) pathway. The aim of this study was to evaluate the association between ApE1 —656T>G and 1349T>G polymorphisms and the susceptibility to breast cancer in northern Iran population. Samples were obtained from 150 patients diagnosed with breast cancer and 150 control subjects and genotyped by tetra - primer amplification refractory mutation system PCR (T-ARMS—PCR). We observed a significant difference in genotype distributions of —656T>G polymorphism between patients and controls (P= 0.03). Findings indicated that individuals with the variant TG genotypes had a significant decreased risk of breast cancer (OR= 0.55, 95% CI= 0.33 - 0.91, P= 0.019). However, the significant association between 1349T>G polymorphism and breast cancer risk was not observed (P = 0.89). Larger studies are needed to confirm our results.
    No preview · Article · Aug 2015 · Cellular and molecular biology (Noisy-le-Grand, France)
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    ABSTRACT: Multiple sclerosis (MS) lesions are characterized by inflammatory demyelination and reactive gliosis, and although remyelination occurs in some lesions it is limited and incomplete. Leukemia inhibitory factor (LIF) is an important cytokine that stimulates oligodendrocyte proliferation and survival in vitro. Opalin is a unique molecular marker for mature oligodendrocytes. The aim of this study was to demonstrate the role of LIF on Opalin and myelin oligodendrocyte glycoprotein (MOG) expression in the cerebral cortex of cuprizone-induced MS mice. The mice were treated with cuprizone for five weeks in order to induce MS. The mice were then divided into 3 groups. The first group was injected intraperitoneally (IP) with LIF for six weeks in the amount of 30 µg/kg bw per day. The second group (SHAM) was injected IP with normal saline and the third group was left without injection as a control. After six weeks the mice were killed, the cerebral cortex was harvested, and the expression of MOG and Opalin was studied. Using western blotting we found that LIF increases Opalin and MOG expression in the cerebral cortex extracts as compared to SHAM and control groups. However, no significant difference in the Opalin and MOG expression was seen between SHAM and control groups. It is concluded that LIF may have an important role in the process of remyelination by increasing Opalin expression and MOG expression.
    No preview · Article · Jul 2015
  • Z Salehi · S Afzali · S Shabanipour · A Rahimi
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    ABSTRACT: Breast cancer (BC) is one of the most frequent cancers among women worldwide. It is a complex polygenic disorder that genetic factors play an important role in disease etiology. The highly significant association of the Fibroblast growth factor receptor 2 (FGFR2) locus with breast cancer risk has been replicated in multiple genome association study; however, it's mechanism of action remains unclear. The aim of this study was to investigate whether the single nucleotide polymorphism (SNP) C—906T within intron 2 of FGFR2 is responsible for susceptibility to breast cancer. This case-control study included 108 breast cancer cases and 108 cancer-free controls. The prevalence of genotype frequencies of the FGFR2 CC/CT/TT was 5.5%, 90.7% and 3.7%, respectively, in cancer cases. Among controls, the distribution of CC, CT and TT genotype was 48.14%, 47.66% and 10.18% respectively. Significant differences in allele and genotype distribution among controls and patients were found (OR, 18.87 95% CI, 7.55—47.16; p = 0.0001). The results from this study suggest that the FGFR2 C—906T polymorphism may be associated to breast cancer in population studied. Well—designed studies with larger sample sizes are needed to confirm the role of FGFR2 SNP in breast cancer risk.
    No preview · Article · May 2015 · Cellular and molecular biology (Noisy-le-Grand, France)
  • F Mashayekhi · S Behrouzi · M Yousefi · Z Salehi
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    ABSTRACT: Infertility is a disease of the reproductive system defined by the failure to achieve a clinical pregnancy after 12 months of regular unprotected sexual intercourse. Both environmental and genetic factors are involved in female infertility. Paraoxonase (PON) is an oxidant enzyme which plays an important role in various diseases and is associated with inflammation, oxidative stress and lipid metabolism. The present study was aimed to evaluate the PON1 192 Q/R gene polymorphism in female infertility. Samples were obtained from 150 patients diagnosed with female infertility and 150 controls subjects and genotyped by Polymerase Chain Reaction—Restriction Fragment Length Polymorphism (PCR—RFLP). The PON genotype frequencies amongst the 70 cases were C/C=40%, C/T=52.8% and T/T=7.2%; the C and T allele frequencies were 66% and 34%, respectively. The PON genotype frequencies amongst the 73 controls were C/C=45.20%, C/T=50.70% and T/T=4.1%; the C and T allele frequencies were 70% and 30%, respectively. We observed a significant difference in the genotype distributions of PON1 192 Q/R polymorphism between patients and controls (P= 0.03). Our findings revealed that individuals with the variant QR had a significant decrease risk of female infertility (OR= 0.55, 95% CI= 0.33 - 0.91, P= 0.019). The data from this study indicates that the PON1 192 Q/R polymorphism may be associated with decreased risk of female infertility. Although more studies should be considered with larger number of patients and control subjects to confirm our results.
    No preview · Article · May 2015 · Cellular and molecular biology (Noisy-le-Grand, France)
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    Full-text · Dataset · May 2015
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    ABSTRACT: Diabetic retinopathy (DR) is the most common microvascular complication of diabetes and the leading cause of blindness in industrialized countries. Oxidative stress plays an important role in the development of microangiopathic complications in diabetes. Manganese superoxide dismutase (MnSOD) is a key mitochondrial antioxidant enzyme in the cellular defense against agents that induce oxidative stress. The aim of the present study was to assess whether the MnSOD A16V (C47T) polymorphism is associated with Diabetic retinopathy in northern Iran. 140 patients with Diabetic retinopathy and 140 healthy individuals, aged 30 to 75 years old, participated in this study. For genotyping of the MnSOD A16V polymorphism the polymerase chain reaction—restriction fragment length polymorphism (PCR-RFLP) method was used. The prevalence of genotype frequencies AA, AV, VV in Diabetic retinopathy subjects were 5.71, 71.43 and 22.86%, respectively, while in controls they were 21.43, 38.57 and 40%, respectively. A significantly increased frequency of the AV genotype was observed in patients as compared with controls (OR = 6.94, 95% CI = 2.98-16.20, P < 0.0001). In conclusion, it is suggested that the MnSOD A16V polymorphism may be associated with the risk of Diabetic retinopathy in northern Iran. However, larger population-based studies are needed for clarifying the relation between Diabetic retinopathy and the MnSOD A16V polymorphism.
    No preview · Article · Apr 2015 · Molecular Biology
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    ABSTRACT: In spite of variety studies in understanding of human reproductive and fertility, the underlying causes of male infertility remains undefined in about 50 % of cases. The polymorphism studies have a crucial role in diseases recognizing. Human apurinic/apyrimidinic endonuclease 1 (ApE1) is a multifunctional protein that has an important role in the base excision repair pathway. The present study was aimed to evaluate whether two polymorphisms -656T>G and 1349T>G ApE1 are related with the susceptibility to idiopathic male infertility. Samples were collected from 180 patients diagnosed with idiopathic male infertility and 120 control subjects and genotyped by tetra-primer amplification refractory mutation system PCR. We observed a significant difference in genotype distributions of -656T>G ApE1 polymorphism between infertile patients and controls (P = 0.0001). Our findings indicated individuals with the variant TG genotypes had a significant increased risk of idiopathic male infertility (OR 1.84, 95 % CI 1.09-3.11, P = 0.021), whereas the significant association between the 1349T>G polymorphism and idiopathic male infertility risk was not observed (P = 0.2). Our data suggest that the -656T>G ApE1 polymorphism may be associated with increased risk of idiopathic male infertility. Larger studies with more patients and controls are needed to confirm the results.
    Full-text · Article · Apr 2015 · International Urology and Nephrology
  • S. P. Hadiyan · F. Mashayekhi · Z. Salehi

    No preview · Article · Apr 2015 · Molecular Biology
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    ABSTRACT: Peptic ulcer disease is a common illness, affecting a considerable number of people worldwide, and its occurrence can be influenced by environmental and genetic factors. Heat shock proteins (HSPs) function mostly as molecular chaperones, and are induced by various stresses. The A to G transition at position 1267 of the HSPA1B gene was shown to correlate with changes in the level of HSPA mRNA expression. Here, the relation between A1267G polymorphism of the HSPAIB gene and risk of peptic ulcer in the Iranian population was evaluated. One hundred subjects, who underwent gastroscopy, took part in the study. DNA samples extracted from the biopsy tissues were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). After gastroscopy, peptic ulcer was diagnosed for 50 patients; among them the distribution of AA/AB/BB genotypes was 10, 88 and 2%, respectively. As for the other 50 subjects (without peptic ulcer) included in the control group, the AA/AB/BB genotypes were identified as 40, 52 and 8%, respectively. A significant association was found between the HSPA1B genotype and peptic ulcer (6.76 OR; 95% CI, 2.26-20.2; p = 0.0006). Thus, the HSPA1B A1267G polymorphism may be a marker of susceptibility to peptic ulcer.
    No preview · Article · Apr 2015 · Molekuliarnaia biologiia

  • No preview · Article · Jan 2015 · Molekuliarnaia biologiia
  • S. P. Hadiyan · F. Mashayekhi · Z. Salehi

    No preview · Article · Jan 2015 · Molekuliarnaia biologiia
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    ABSTRACT: Background: Development of gastric cancer (GC) is a multistep process that requires alterations in the expression of oncogenes and tumor suppressor genes, occurring over several decades. The p53 tumor suppressor protein is involved in cell-cycle control, apoptosis and DNA repair. One of the most important regulators of p53 is MDM2, which acts as a negative regulator in the p53 pathway. Based on the key role of p53 and MDM2 in tumor suppression, polymorphisms that cause change in their function might affect cancer risk. We therefore elevated associations of the polymorphisms of p53 (R72P) and MDM2 (SNP309) with GC in Iran. Materials and methods: A total of 104 patients with gastric cancer and 100 controls were recruited. Genomic DNA was extracted from fresh gastric samples. Genotyping of the p53 and MDM2 genes was performed using allele specific PCR (AS-PCR). Results: There was no significant difference between the p53 codon 72 polymorphism distribution in control and patient groups (p=0.54), but the G allele of MDM2 was found to be over-represented in patients (p=0. 01, Odds Ratio=2. 08, 95% Confidence Interval= 1.37-4.34). Conclusions: The p53 R72P seems not to be a potential risk factor for development of GC among Iranian patients, but our data suggest that MDM2 SNP309 might modify the risk related to GC.
    No preview · Article · Sep 2014 · Asian Pacific journal of cancer prevention: APJCP
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    ABSTRACT: Endometriosis is defined as the presence of ectopic endometrial glands and stroma outside of the uterine cavity. Recent studies have shown that the oxidative stress causes irreparable damage, which leads to oxidative enzymopathies. Catalase gene encodes an antioxidant enzyme, detoxifying hydrogen peroxide to H2O and O2. The aim of this study was to determine whether the polymorphism at position -262 in the promoter region of catalase gene (C-262T), which alters the expression and enzyme blood levels, could have an impact on the risk of endometriosis. Extracted DNA from peripheral blood leucocytes was genotyped using allele-specific PCR (AS-PCR). The χ(2)-test was used for statistical analyses. In endometriosis subjects, the frequencies of the CAT CC/CT/TT were 67.5%, 32.5% and 0%, respectively, while in healthy women, they were 12%, 68% and 20%, respectively. Significant differences in allele and genotype distribution among controls and patients were found (OR, 178.76 95% CI, 10.11-3159.1202; p = 0.0004). This study indicates that catalase C-262T polymorphism is associated with the endometriosis. Randomised multicentre trials with greater sample sizes are still needed to clarify our results.
    No preview · Article · Aug 2014 · Journal of Obstetrics and Gynaecology
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    ABSTRACT: Spontaneous abortion is the most common complication of early pregnancy. Genetic factors have been hypothesised to play a role in spontaneous abortion. Since it is possible that the balance of oxidants and antioxidants can be affected by different genetic variants, gene polymorphisms have been proposed as a susceptibility factor that increases the chance of abortion. Manganese superoxide dismutase is an important antioxidant enzyme encoded by manganese superoxide dismutase (MnSOD) gene. The aim of this experiment was to assess whether Val16Ala polymorphism of MnSOD gene is associated with abortion in north of Iran. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for genotyping. Statistical analyses were conducted using the χ(2)-test. The genetic distributions did not differ significantly between cases and controls, however slightly more Val/Val genotypes were found among the patients compared with control subjects (p = 0.059). No correlation was observed between susceptibility to abortion and MnSOD Val16Ala polymorphism. Larger population-based studies are needed for clarifying the relationship between abortion and MnSOD genotypes.
    Full-text · Article · Aug 2014 · Journal of Obstetrics and Gynaecology
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    ABSTRACT: Abstract About 10%-15% of conceptions are lost spontaneously prior to 20 weeks. Apart from the clinical problems, genetic variations have also been proposed as a susceptibility factor to miscarriage. Glutathione peroxidase 1 (GPX1) and catalase (CAT) encode two antioxidant enzymes that detoxify H2O2 and protect the cells from oxidative damage. A functional polymorphism at codon 198 of the GPX1 gene causes a C/T substitution in exon 2, which encodes for either proline or leucine (Pro198Leu). The CAT gene has a polymorphic site in the promoter region at position -262 (C-262T) which alters the expression and enzyme blood levels, leading to some pathological clinical conditions. In this study, we evaluated the association of these two polymorphisms with the risk of spontaneous abortion. Genomic DNA from 105 cases with spontaneous abortion and 90 healthy women were genotyped using allele-specific PCR (AS-PCR) and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The genetic distributions for GPX1 did not differ significantly between cases and controls (p = 0.680). However, C-262T polymorphism was significantly associated with the risk of the disease (OR, 5.50; 95% CI, 1.43-21.09; p = 0.012). In conclusion, this study indicates that CAT -262T/T genotype confers less susceptibility to spontaneous abortion, while GPX1 Pro198Leu polymorphism may not be correlated with the disease.
    Full-text · Article · Jul 2014 · Systems Biology in Reproductive Medicine
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    ABSTRACT: Implantation failure is a major limiting step for in-vitro fertilisation (IVF). Embryo implantation is the result of the interaction of the embryo with the endometrium. Oxidative stress (OS) can cause defective embryo development and retardation. Genetic polymorphisms of detoxicating enzymes, such as glutathione S-transferases (GSTs), may play an important role in the outcome of embryo implantation. GSTM1 and GSTT1 are known to be highly polymorphic. The aim of this study was to examine the association of GSTM1 and GSTT1 gene polymorphisms with IVF-ET outcome in a population in northern Iran. Blood samples were collected from 120 infertile women who underwent an IVF cycle, and 108 healthy volunteers. Genomic DNA was prepared from peripheral blood leucocytes. Genotype frequencies were determined in patients and healthy controls using polymerase chain reaction (PCR). It was found that 25.8% of the infertile women and 0% of the controls had the GSTM1 null genotype (odds ratio (OR) = 76.37; 95% CI = 4.6-1,265.7; p = 0.0025). On the other hand, 5% of the cases and 0% of the controls had the GSTT1 null genotype (OR = 12.3, 95% CI = 0.68-221/3, p = 0.088). These results suggest that GSTM1 null type might be associated with IVF outcome in a population in northern Iran.
    No preview · Article · Jul 2014 · Journal of Obstetrics and Gynaecology
  • Mikhak Jahantab · Mohammad Haseli · Zivar Salehi
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    ABSTRACT: Recently, it has been shown that many nematode species are in fact species complex, using exact morphological and genetic studies. In this case, there are no such studies related to the genus Raphidascaris Railliet & Henry, 1915. Herein, the morphological and genetic variations among the Iranian population of the species Raphidascaris acus (Bloch, 1779) Railliet & Henry, 1915 and the other allopatric populations with morphological and genetic information were compared to show whether this species can be considered as a species complex. R. acus is an anisakid species and has been frequently reported from different host species from the Caspian Sea. Nonetheless, there are no morphological and genetic information for this species from the region. In the present study, a total of 20 specimens of R. acus were collected from Esox lucius Linnaeus, and the morphology of the Caspian population of this species was surveyed for the first time using both light and scanning electron microscopy. Meanwhile, some parts of ribosomal DNA (rDNA) including internal transcribed spacer 1 (ITS1), 5.8 s, and ITS2 were sequenced and presented as the genetic marker for this species. To understand whether R. acus can be considered as a species complex, the Caspian population of this species was compared morphologically with the allopatric populations of Czech and Canada and genetically with the allopatric population of Poland (Vistula lagoon). Morphologically, there was no difference between the Caspian and Czech populations, but the Caspian and Canadian populations differed in the length of ejaculatory duct and the presence of small triangular elevation between the bases of subventral lips. The nucleotide difference between the Caspian and Polish populations was 4.48 %. In comparison with the interspecific genetic distances in the genus Raphidascaris, this value is notable. In conclusion, based on morphological and genetic differences among the allopatric populations of R. acus, this species is probably a species complex. Nonetheless, the definitive taxonomic decision in recognizing R. acus as a species complex and the description of its sibling species depend on surveying other allopatric populations morphologically and genetically accompanied by an evaluation of reproductive isolation among them.
    No preview · Article · Jul 2014 · Parasitology Research
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    S Sabouhi · Z Salehi · M H Bahadori · M Mahdavi
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    ABSTRACT: Infertility is the failure of a couple to engender after endeavouring at least one full year of unprotected intercourse. It has been reported that reactive oxygen species contributed to pathogenesis of various disease. To inactivate ROS cells biosynthesise several antioxidant enzymes, one of them is catalase which contributes H2 O2 to H2 O and O2 . This study set out to delineate the association of catalase C-262T polymorphism with idiopathic male infertility. The study included 195 men with idiopathic infertility and 190 healthy volunteers. Genomic DNA was extracted from peripheral blood leucocytes. Genotype and allele frequencies were determined in patients and controls using allele-specific PCR (AS-PCR). The prevalence of genotype frequencies of the CAT CC/CT/TT was 31.79%, 65.12% and 3.07%, respectively, in infertile subjects, as against 24.73%, 55.26% and 20%, respectively, in healthy volunteers. Statistical analysis has emerged significant difference from the comparison of either genotype (P < 0.05). Taking into accounts of results, the catalase C-262T polymorphism indicates that CAT-262T/T genotype confers less susceptibility to male infertility. Further studies with larger numbers of patients are required for further evaluation and confirmation of our finding.
    Full-text · Article · Jan 2014 · Andrologia

Publication Stats

435 Citations
102.46 Total Impact Points

Institutions

  • 2004-2015
    • University of Guilan
      • Department of Biology
      Resht, Gīlān, Iran
  • 2009-2011
    • Islamic Azad University - Tonekabon Branch
      Shahsavār, Māzandarān, Iran
    • Guilan University of Medical Sciences
      Resht, Gīlān, Iran