[Show abstract][Hide abstract] ABSTRACT: Human colorectal cancer cells were incubated with medium containing 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU). The cells synthesized Xyl-MU-derivatives which were detected in the culture medium by gel-filtration high-performance liquid chromatography. These included a Xyl-MU-induced glycosaminoglycan and its biosynthetic intermediates, Galbeta1-4Xylbeta1-MU and Galbeta1-3Galbeta1-4Xylbeta1-MU, and other Xyl-MU-induced oligosaccharides, not related to Xyl-MU-induced glycosaminoglycan, were also synthesized. One of these oligosaccharides, sulfate-O-3GlcAbeta1-4Xylbeta1-MU, reacted with HNK-1, a mouse monoclonal antibody raised against human natural killer cells. Human neural cells and skin fibroblasts have also been reported to synthesize HNK-1-reactive sugar chains. Since HNK-1-reactive sugar chains are known to be involved in cell adhesion in the nervous system, the present results suggest that epithelium-derived colorectal cancer cells might also be able to utilize them in cell adhesion.
No preview · Article · Feb 2003 · The Tohoku Journal of Experimental Medicine
[Show abstract][Hide abstract] ABSTRACT: Human colorectal cancer cells were incubated with medium containing 4-methylumbelliferyl-beta-D-Xyloside (Xyl-MU). The cells synthesized Xyl-MU-derivatives which were detected in the culture medium by gel-filtration high-performance liquid chromatography. These included a Xyl-MU-induced glycosaminoglycan and its blosynthetic intermediates, Galbeta1-4Xylbeta1-MU and Galbeta1-3Galbeta1-4Xylbeta1-MU, and other Xyl-MU-induced oligosaccharides, not related to Xyl-MU-induced glycosaminoglycan, were also synthesized. One of these oligosaccharides, sulfate-O-3GlcAbeta1-4Xylbeta1-MU, reacted with HNK-1, a mouse monoclonal antibody raised against human natural killer cells. Human neural cells and skin fibroblasts have also been reported to synthesize HNK-1-reactive sugar chains. Since HNK-1-reactive sugar chains are known to be involved in cell adhesion in the nervous system, the present results suggest that epithelium-derived colorectal cancer cells might also be able to utilize them in cell adhesion.
No preview · Article · Jan 2003 · The Tohoku Journal of Experimental Medicine
[Show abstract][Hide abstract] ABSTRACT: We studied the clinical significance of histological heterogeneity (the histological combination in a single tumor) in colorectal cancer, and examined immunohistologically the expression of urokinase-type plasminogen acticator (uPA). The patients were less than 75 years, and had undergone curative surgery for advanced colorectal cancer. Their cases were investigated for the relation between histological heterogeneity and clinicopathological factors, and the influence on disease free 5-year survival rate and recurrent form. Furthermore, uPA expression was examined immunohistologically. The positive cases of histological heterogeneity showed poor prognoses in disease free 5-year survival rate. Histological heterogeneity related to local recurrence in colon cancer. UPA showed no significance in relation to histological heterogeneity. We speculated that the histological heterogeneity reflected biological malignancy of tumors and proved to be an important prognosis detecting factor.
No preview · Article · Jan 2000 · Nippon Shokaki Geka Gakkai zasshi
[Show abstract][Hide abstract] ABSTRACT: It is important to evaluate the degree of hepatic fibrosis when diagnosing and treating hepatic cirrhosis. We focused on hydroxyproline, which is detected specifically in collagen, which plays a major role in hepatic fibrosis. The correlations between liver tissue hydroxyproline residue levels and the degree of hepatic fibrosis were examined in dogs with dimethylnitrosamine-induced fibrotic livers.
Dimethylnitrosamine was administered to dogs to establish experimental hepatic fibrosis. Paraffinized sections of liver specimens, stained with hematoxylin-eosin and azan, were examined and the degree of hepatic fibrosis was graded. About three-milligram samples of liver tissue were loaded onto a fully automated liquid chromatograph and the levels of hydroxyproline residues were measured.
The liver tissue hydroxyproline appeared to reflect the degree of hepatic fibrosis. The liver tissue hydroxyproline levels and pathological hepatic fibrosis grades correlated significantly (p<0.05).
Tissue hydroxyproline appears to be a more useful fibrosis marker, because hydroxyproline is influenced less by other factors. Furthermore, our results demonstrate that a very small amount of liver tissue (wet weight 3 mg) was enough to enable the levels of hydroxyproline residues to be measured by an automated amino acid analyzer (JLC-3000) and hepatic fibrosis is expressed as the numerical value by this analysis.
No preview · Article · Nov 1998 · Hepato-gastroenterology
[Show abstract][Hide abstract] ABSTRACT: Two cases of postoperative abnormal prothrombinemia presumably caused by the administration of cefoperazone are herein described. One patient, who had bile duct cancer with obstructive jaundice, underwent resection of the extrahepatic bile duct with hepaticojejunostomy (Roux-en-Y anastomosis) and partial resection of the liver following percutaneous transhepatic cholangial drainage. He developed abnormal prothrombinemia and bleeding 10 days after surgery. The other patient, who had undergone a total gastrectomy 17 years earlier, suffered from pulmonary tuberculosis. She was initiated anti-tuberculous regimen and simultaneously was worked-up for her severe anemia, and was found to have ascending colon cancer. She underwent a right hemicolectomy, cholecystectomy, and repair of ventral incisional hernia, and subsequently developed abnormal prothrombinemia and bleeding 12 days after surgery. Both patients received a chemical bowel preparation prior to surgery. Prothrombin time was normal preoperatively in both patients. Both patients were treated with fresh frozen plasma and intravenous menatetrenon, which improved the clotting disorder within 24h. Antibiotics containing the N-methyl-thio-tetrazol side chain should thus be used with particular prudence in patients with abnormal prothrombinemia and a tendency to develop bleeding disorders.
[Show abstract][Hide abstract] ABSTRACT: This case was a 61-year-old male diagnosed with pancreatic cancer who had a celiotomy. The tumor invasion involved the pancreas body and head. Ultrasonograph examination revealed that all the celiac artery, supramesenteric artery, and portal vein were occluded in the tumor. Because of the unresectability of the tumor, a palliative operation was carried out, and during the following 9 months he was given UFT 300 mg daily. Because abdominal ascites accompanied the tumor growth, the patient underwent combination chemotherapy of cisplatin 5 mg/day x 5/week and continuous infusion of 5-fluorouracil 250 mg/day for 4 weeks. Remarkable reduction of the abdominal ascites and decline of the tumor markers (CEA, CA19-9) was observed in the course of the chemotherapy. Bone marrow function was suppressed by the agents, but granulocyte colony stimulating factor (G-CSF) was very effective for recovery from the damage. Two months after discharge, abdominal ascites recurred. The patient received the same serial chemotherapy for 6 weeks, and now is followed as an outpatient.
No preview · Article · Jul 1997 · Gan to kagaku ryoho. Cancer & chemotherapy
[Show abstract][Hide abstract] ABSTRACT: Transduodenal sphincteroplasty is designed to destroy the sphincteric muscle fibers, producing a terminal choledochoduodenostomy. In the absence of Oddi's sphincter, intestinal contents with both activated pancreatic juice and bacterial flora are refluxed into the bile duct and remain there for a prolonged time. The long-term effect of producing the reflux has not been evaluated to date.
One hundred nineteen consecutive patients undergoing transduodenal sphincteroplasty between February 1973 and July 1984 were included in this study. Postoperative clinical courses of 108 patients could be evaluated by means of a retrospective review of the hospital records. Median follow-up was 18 years.
Eight cases (7.4%) of primary bile duct cancer were found among the 108 cases at intervals of 1 to 20 years after sphincteroplasty. Two patients had concurrent hepatolithiasis. The patency of sphincteroplasty was confirmed in all cases, and the bile was infected in seven cases. Pathologic specimens obtained demonstrated cholangiocarcinomas and various degrees of atypical hyperplastic lesions under the background of chronic cholangitis.
Chronic cholangitis can be an important causative factor in late development of bile duct cancer after sphincteroplasty. Any patients treated with choledochoduodenostomy should be closely monitored for late cholangiocarcinoma.
[Show abstract][Hide abstract] ABSTRACT: Liver regeneration after partial hepatectomy is accompanied by hepatocyte proliferation and alteration of the extracellular matrix. Glycosaminoglycans, which are components of the extracellular matrix, interact with other matrix components, and are related to hepatocyte growth. The aim of this study was to investigate the relationship between hepatocyte proliferation and changes in glycosaminoglycan.
Hepatocyte proliferation and changes in glycosaminoglycan were investigated in dogs after 55% partial hepatectomy. Hepatocyte mitosis was investigated by immunohistochemistry using anti-proliferating cell nuclear antigen antibody. The amount of glycosaminoglycan was determined by the carbazole-sulfuric acid method. We used a new method for analysis of glycosaminoglycan chains, involving endo-beta-xylosidase digestion and fluorescence labelling, to investigate the components of glycosaminoglycan.
Hepatocyte mitosis was increased after hepatectomy, reaching a peak at postoperative day 7. The total amount of hepatic glycosaminoglycan reached a maximum at 1 to 2 weeks afer hepatectomy, and the ratio of the components showed a concomitant change, the amount of heparan sulfate increasing, and that of chondroitin sulfate/dermatan sulfate decreasing. Increased heparan sulfate has shorter chains at 1 to 2 weeks after hepatectomy.
These results suggest that the transient changes in heparan sulfate with a decreased chain length and chondroitin sulfate/dermatan sulfate and observed during liver regeneration are associated with hepatocyte proliferation.
No preview · Article · Jun 1997 · Journal of Hepatology
[Show abstract][Hide abstract] ABSTRACT: Donor livers with massive fatty infiltration reportedly are susceptible to ischemia/reperfusion injury after transplantation, which contributes to risk of primary nonfunction. We investigated the effect of warm ischemia and reperfusion on sinusoidal microcirculation in rats with fatty livers from a choline-deficient diet. Rats were subjected to partial hepatic warm ischemia for 30, 60, or 90 min. In a second study, an anti-ICAM-1 monoclonal antibody was injected intraportally 2 min after a 60-min ischemic period. In both studies, injury was assessed by liver histology 6 hr after vascular clamp release and by animal survival. After 30 min of hepatic warm ischemia, almost all control and fatty-liver rats survived 7 days. After 60-min ischemia, however, survival was significantly less in rats with fatty livers than in controls with normal livers (10% vs 90%, P < 0.0001). Histologically, rats with fatty livers showed marked sinusoidal congestion, especially in the midzone of the acinus, while control rats showed no disturbance in microcirculation. In rats with fatty livers treated with intraportal injection of an anti-ICAM-1 antibody, sinusoidal microcirculation was well preserved and the 7-day survival rate after warm ischemia was improved (50% vs no antibody 10%; P = 0.0112). In fatty livers, midzonal sinusoidal flow block occurs after hepatic warm ischemia and reperfusion. Although intraportal injection of an anti-ICAM-1 monoclonal antibody corrected this microcirculatory failure, animal survival was not as good as for controls without fatty livers. These results suggest that both sinusoidal microcirculatory failure and ischemic hepatocellular damage contribute to warm ischemia/reperfusion injury in fatty livers.
No preview · Article · Jun 1997 · Journal of Surgical Research
[Show abstract][Hide abstract] ABSTRACT: To study the role played by hepatic bile mucus glycoprotein in the development of hepatolithiasis, mucus glycoprotein, isolated from the bile of patients with intrahepatic gallstones by gel filtration and ultracentrifugation, was examined for precipitability in control hepatic bile obtained postoperatively from patients successfully treated for cholecysto- and/or choledocho-lithiasis. When the mucus glycoprotein was incubated at 38 degrees C for 48 h in the control hepatic bile in the presence of calcium ions, massive precipitation was produced. The precipitation was inhibited by treating the mucus glycoprotein with acid, alkali, a reducing reagent, or protease, the inhibition being most effective with acid, which splits up carbohydrate chains. This suggests that the precipitability of the mucus glycoprotein resides mainly in its carbohydrate chains. These observations imply that the development of intrahepatic gallstones, calcium bilirubinate stones in particular, could be prevented by degrading mucus glycoprotein in hepatic bile.
No preview · Article · Jan 1996 · Journal of Gastroenterology