[Show abstract][Hide abstract] ABSTRACT: To investigate the outcomes of pancreas-sparing duodenectomy (PSD) with regional lymph node dissection vs pancreaticoduodenectomy (PD).
Between August 2001 and June 2014, 228 patients with early-stage ampullary carcinoma (Amp Ca) underwent surgical treatment (PD, n = 159; PSD with regional lymph node dissection, n = 69). The patients were divided into two groups: the PD group and the PSD group. Propensity scoring methods were used to select patients with similar disease statuses. A total of 138 matched cases, with 69 patients in each group, were included in the final analysis.
The median operative time was shorter among the patients in the PSD group (435 min) compared with those in the PD group (481 min, P = 0.048). The median blood loss in the PSD group was significantly less than that in the PD group. The median length of hospital stay was shorter for patients in the PSD group vs the PD group. The incidence of pancreatic fistula was higher among patients in the PD group vs the PSD group. The 1-, 3-, and 5-year overall survival and disease-free survival rates for patients in the PSD group were 83%, 70%, 44% and 73%, 61%, 39%, respectively, and these values were not different than compared with those in the PD group (P = 0.625).
PSD with regional lymph node dissection presents an acceptable morbidity in addition to its advantages over PD. PSD may be a safe and feasible alternative to PD in the treatment of early-stage Amp Ca.
[Show abstract][Hide abstract] ABSTRACT: A family of 2,9-di(3-R-1H-imidazolium-1-yl)-1,10-phenanthroline iodides and hexafluorophosphates (R = allyl, benzyl, mesityl, picolyl) were synthesized from 2,9-diiodophenanthroline and imidazole or N-substituted imidazoles. Simple reactions of these diimidazolium salts with copper powder at room temperature have afforded a series of multinuclear copper(I)-NHC complexes in good yields. The structures vary depending on the N substituents and counterions. [Cu4(L1)2(MeCN)4](PF6)4 (R = allyl) exhibits a zigzag Cu4 chain with two terminal [Cu(NHC)(MeCN)2] and two internal [Cu(phen)(NHC)] moieties. [Cu3(L2)2](PF6)3 (R = benzyl) contains a strictly linear Cu3 framework with two [Cu(NHC)2] units and a [Cu(phen)2] located at the center. Both complexes [Cu3(L4)2](PF6)3 (R = mesityl) and [Cu3(L5)2](PF6)3 (R = picolyl) consist of a triangular Cu3 core in which two copper(I) ions are surrounded by a phen and a NHC group and the third copper(I) is coordinated by two NHC groups. [Cu3(L3)2](PF6)3 derived from 2,9-di(3-benzyl-1H-benzimidazolium-1-yl)-1,10-phenanthroline hexafluorophosphate can undergo transannulation of the benzimidazolylidene ring giving [Cu2(L3')2](PF6)2. The decomposition process might involve solvent-induced rearrangement of the ligand and hydrolysis of carbene moieties. Treatment of 2,9-di(3-mesityl-1H-benzimidazolium-1-yl)-1,10-phenanthroline iodide with copper powder generated dinuclear complex [Cu2(L4)2][Cu2(μ-I)2I2] (R = mesityl) possessing a macrocyclic cation and [Cu2(μ-I)2I2](2-) anion. Tetranuclear complex [Cu4(L5)2(μ-I)2](CuI3) (R = picolyl) has been obtained from its diimidazolium iodide and copper powder. All Cu(I) complexes have been characterized by X-ray single diffraction analysis, elemental analysis, and NMR spectra. Their redox behavior and fluorescent properties have also been studied.
No preview · Article · Sep 2014 · Inorganic Chemistry
[Show abstract][Hide abstract] ABSTRACT: This paper is aimed to assess the effects of red blood cell (RBC) transfusion on clinical outcomes in cardiac surgery. Trials were identified by computer searches of the Pubmed, MEDLINE, Cochrane Library (Issue 10, 2012), from January 1980 to October 2012. References in identified trials and review articles were checked and experts contacted to identify any additional trials. The homogeneous randomized controlled trials (RCTs) were analyzed with RevMan 5.1 software. Five trials involving a total of 1,203 patients were identified. The results of meta-analyses showed that restrictive transfusion strategies reduced the risk of receiving a RBC transfusion (MD = - 1.46, 95% CI -1.18(-) -1.1) and the volume of RBCs transfusion (RR = 0.69, 95% CI 0.53-0.89). No significant difference was noted between the two strategies in terms of mortality, adverse events and hospital or intensive care length of stay. Based on the results mentioned above, one can draw a conclusion that restrictive transfusion strategies reduced the risk of receiving RBC transfusion and the volume of RBCs transfused. Restrictive transfusion strategies did not appear to impact on the rate of adverse events and hospital or intensive care length of stay, compared to liberal transfusion strategies.
No preview · Article · Apr 2014 · Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
[Show abstract][Hide abstract] ABSTRACT: Despite advances in immunosuppressive drugs, long-term success of liver transplantation is still limited by the development of chronic liver allograft dysfunction. Although the exact pathogenesis of chronic liver allograft dysfunction remains to be established, there is strong evidence that chemokines are involved in organ damage induced by inflammatory and immune responses after liver surgery. Chemokines are a group of low-molecular-weight molecules whose function includes angiogenesis, haematopoiesis, mitogenesis, organ fibrogenesis, tumour growth and metastasis, and participating in the development of the immune system and in inflammatory and immune responses. The purpose of this review is to collect all the research that has been done so far concerning chemokines and the pathogenesis of chronic liver allograft dysfunction and helpfully, to pave the way for designing therapeutic strategies and pharmaceutical agents to ameliorate chronic allograft dysfunction after liver transplantation.
Preview · Article · Dec 2013 · Clinical and Developmental Immunology
[Show abstract][Hide abstract] ABSTRACT: To investigate the monitoring of renal microcirculation perfusion alteration with contrast-enhanced ultrasound (CEU) during cardiopulmonary bypass (CPB).
Renal microcirculation perfusion before and during CPB was assessed by CEU. Time-intensity curve (TIC) were derived from three region of interest (ROI) using QLAB post-analysis software. Parameters such as TIC curve wash in slope (A), area under curve (AUC), peak intensity (DPI) as well as time to peak intensity (TTP) were then calculated.
Contrast-enhanced ultrasound showed a significant reduction (or elongation) of perfusion parameter (A, AUC, DPI, TTP) in all three regions (superficial cortex, deep cortex and medulla) during cardiopulmonary bypass in comparison with normal cardiac cycle.
Cardiopulmonary bypass (CPB) has a dramatic impact on renal microcirculation in pediatric patients which manifest as global reduction in renal perfusion as well as significant region perfusion difference. Contrast-enhanced ultrasound (CEU) could detect the renal microcirculation alteration during CPB.
No preview · Article · Jul 2013 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
[Show abstract][Hide abstract] ABSTRACT: We used 3-dimensional computed tomographic images to create a disease-based transesophageal echocardiographic simulation system for complex congenital heart defects. We enrolled 7 pediatric patients with complex congenital heart defects in this proof-of-concept study. Preoperative computed tomographic images and intraoperative transesophageal echocardiographic images were acquired for all patients. Two- and 3-dimensional computed tomographic cross-sectional images were created to simulate the process of transesophageal echocardiographic image acquisition. Computed tomographic images simulating the midesophageal 4- and 5-chamber views, aortic valve short-axis views, long-axis views, and ascending aortic short-axis views were created to correspond with the actual transesophageal echocardiographic images from each patient. Four reviewers then evaluated the image quality of the computed tomographic images, the agreement between the echocardiographic and tomographic images, and the ability of the 3-dimensional computed tomographic full-volume and cross-sectional images to yield the spatial and temporal congruence of transesophageal echocardiograms. In most of the patients, computed tomography yielded images of good-to-excellent quality. Strong agreement was noted between the computed tomographic and transesophageal echocardiographic images acquired in the same patients. The ability of 3-dimensional computed tomography to yield the spatial and temporal congruence of transesophageal echocardiography in selected planes was also good to excellent. We found that 3-dimensional computed tomographic images can simulate the process of transesophageal echocardiography in acquiring the echocardiographic image clearly. This imaging method has the potential to be applied successfully to a disease-based transesophageal echocardiographic simulation system.
No preview · Article · Jun 2013 · Texas Heart Institute journal / from the Texas Heart Institute of St. Luke's Episcopal Hospital, Texas Children's Hospital
[Show abstract][Hide abstract] ABSTRACT: To evaluate the feasibility of using trans-esophageal echocardiogram (TEE) in dynamic monitoring of renal blood flow (RBF) in pediatric patient undergoing cardiac surgery and to identify potential factors that affect RBF during cardiopulmonary bypass (CPB).
Pediatric patient (n = 46) undergoing open surgical repair for congenital heart defect were recruited in this study. Velocity of RBF and renal artery diameter were measured during surgery for calculating volumetric RBF. Cardiopulmonary bypass and hemodynamic data were recorded.
RBF was measured successfully in 42 patients (91%). Resistance index of renal artery increased significantly after CPB compared to the preoperative stage. A strong linear correlation was found between perfusion volume (Vmean) and RBF. Non-linear correlation was found between mean arterial pressure and RBF.
Perfusion volume is the major factor that determines RBF during CPB. Measuring renal blood flow using TEE in pediatric patient is highly feasible and it can serve as dynamic renal perfusion monitoring during CPB.
No preview · Article · Apr 2013 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
[Show abstract][Hide abstract] ABSTRACT: : Peripheral nerve injuries that provoke neuropathic pain are associated with chronic inflammation and nervous lesions. The authors hypothesized that chronic neuropathic pain might be caused by chronic inflammation resulting from a nervous autoimmune reaction triggered by nerve injury.
: The authors observed chronic inflammation and neuropathic behaviors for up to 12 weeks after nerve injury in T lymphocyte-deficient nude mice and their heterozygous littermates. Lymphocyte proliferation and Schwann cell apoptosis were examined after coculture of each population with various neural tissues from normal rats and those with nerve injury.
: Nude mice recovered faster and exhibited less thermal hyperalgesia after nerve injury compared to their heterozygous littermates. A large number of IL-17 cells indicative of lymphocyte activation were found in the injured sciatic nerve and spinal cord (L4-6) of heterozygous littermates, but far fewer of these populations were found in nude mice. In vitro lymphocyte proliferation was enhanced after coculture with nerve tissues from normal rats compared to nerve tissue-free phosphate-buffered saline controls. In particular, coculture with sciatic nerve tissue enhanced proliferation by 80%, dorsal root ganglion by 46%, and spinal cord by 14%. Moreover, neural tissues from rats with nerve injury markedly increased the lymphocyte proliferation compared to coculture with tissues from corresponding normal rats. Schwann cell apoptosis was triggered in vitro when cocultured with lymphocytes from neuropathic rats.
: Our study suggests that chronic neuropathic pain might be caused by chronic inflammation resulting from a nervous autoimmune reaction triggered by nerve injury.
[Show abstract][Hide abstract] ABSTRACT: To explore the optimal steroid therapeutic strategy for autoimmune pancreatitis (AIP).
This study was conducted retrospectively in two large institutions in China. Patients with clinically, radiologically and biochemically diagnosed AIP were enrolled. The performed radiological investigations and biochemical tests, the regimen of the given steroid treatment, remission and relapse whether with and without steroid therapy were analyzed.
Twenty-eight patients with AIP received steroid treatment, while 40 patients were treated surgically by pancreatoduodenectomy, distal pancreatectomy and choledochojejunostomy, radiofrequency ablation for the enlarged pancreatic head, percutaneous transhepatic biliary drainage and endoscopic biliary drainage. The starting oral prednisolone dose was 30 mg/d in 18 (64.3%) patients and 40 mg/d in 10 (35.7%) patients administered for 3 wk. The remission rate of AIP patients with steroid treatment (96.4%) was significantly higher than in those without steroid treatment (75%). Maintenance therapy (oral prednisolone dose 5 mg/d) was performed after remission for at least 6-12 mo to complete the treatment course. Similarly, the relapse rate was significantly lower in AIP patients with steroid treatment (28.6%) than in those without steroid treatment (42.5%). Steroid re-treatment was effective in all relapsed patients with or without steroid therapy.
Steroid therapy should be considered in all patients with active inflammatory phase of AIP. However, the optimal regimen still should be trailed in larger numbers of patients with AIP.
Preview · Article · Jan 2013 · World Journal of Gastroenterology
[Show abstract][Hide abstract] ABSTRACT: Background:
The noble gas anaesthetic, xenon has previously been shown to protect the adult myocardium from ischaemia/reperfusion (I/R) injury, however its effect on immature myocardium is unclear. The aim of this study was to investigate the effect of xenon on the isolated immature heart.
Isolated, immature (2-3weeks old) New Zealand rabbit hearts were perfused with Krebs-Henseleit buffer via Langendorff-mode. After 20min of baseline equilibration, hearts were pretreated with 75% xenon, 75% xenon+100μM diazoxide, or 75% xenon+100μM 5-hydroxydecanoate, and then subjected to 1h of global ischaemia and 3h of reperfusion.
Pretreatment with 75% xenon significantly improved cardiac function (P<0.01 vs. the I/R group, respectively), limited myocardial infarct size (20.83±2.16%, P<0.01 vs. 35.82±2.14% of the I/R group), reduced cardiac enzyme release (CK-MB, 1.00±0.19IU/L, P<0.01 vs. 0.44±0.14IU/L of the I/R group; LDH, 6.15±1.06IU/L P<0.01 vs. 3.49±0.37IU/L of the I/R group) and decreased apoptosis (6.17±0.56%, P<0.01 vs. 11.31±0.93% of the I/R group). In addition, the mitochondrial structure changes caused by I/R injury were largely prevented by 75% xenon pretreatment (1.37±0.16, P<0.01 vs. 2.32±0.13 of the I/R group). The mitochondrial adenosine triphosphate-sensitive potassium (mitoKATP) channel opener diazoxide did not influence the effect of xenon, but the specific mitoKATP channel blocker 5-hydroxydecanoate completely abolished this effect.
Our study demonstrated that pretreatment with 75% xenon protected immature heart from I/R injury, and this protection was probably mediated by preservation of myocardial mitochondria and opening of mitoKATP channel.
No preview · Article · Dec 2012 · Heart, Lung and Circulation
[Show abstract][Hide abstract] ABSTRACT: The gene encoding a group 3 late embryogenesis abundant (LEA) protein was cloned from callus of the drought-tolerant grass Pogonatherum paniceum. Three alternatively spliced transcripts of this gene were amplified by RT-PCR. According to the bioinformatics analysis, the gene contained three exons and two introns. The PpLEA3.1 transcript which was the most abundant one in P. paniceum contained all three exons, and the PpLEA3.2 transcript lacked fragments of the first two exons which encoded a 41-amino acid-long region of the PpLEA3 protein. The PpLEA3.3 transcript retained the second intron. The three splicing patterns resulted in changes in the number of repeats of an 11-amino acid motif, hydropathy and the predicted 3-dimensional structure. When expressed in Escherichia coli, the three proteins differentially affected growth responses to salt, cold and heat stress. These results confirmed that the complete motif repeat structures and an appropriate hydrophilic/hydrophobic balance are important for the LEA protein in providing protection against various forms of stresses.
Full-text · Article · Aug 2012 · Journal of plant physiology
[Show abstract][Hide abstract] ABSTRACT: Reactions of [FeL2(CH3CN)2]2+ (L = N-pyrimid-2-ylimidazolylidene) with various N-, P-, O-, and S-donors were investigated. By replacing the labile acetonitrile, various iron-NHC complexes containing additional N-, P-, O-, and S-ligands were prepared. All the iron-NHC complexes were fully characterized by NMR spectroscopy and X-ray crystallography. [FeL2(CH3CN)2]2+ could efficiently catalyze the coupling reactions of various Grignard reagents with heteroaryl bromides or chlorides.
Full-text · Article · Jul 2012 · Chinese Science Bulletin
[Show abstract][Hide abstract] ABSTRACT: Transcatheter closure is the mainstay of treatment for patent ductus arteriosus (PDA) in the pediatric patient but it is technically challenging and does not always succeed, especially in a younger age child with a large PDA. We present a technique of using a transesophageal echocardiogram-guided minimally invasive perventricular closure for the pediatric patient with a large PDA who failed transcatheter closure.
No preview · Article · Mar 2012 · The Annals of thoracic surgery
[Show abstract][Hide abstract] ABSTRACT: A postoperative acquired left ventricular (LV) to right atrium (RA) shunt in a pediatric patient is a rare complication, which requires immediate intervention. We report a case of successful management of an acquired LV-RA shunt using an intraoperative closure device.
No preview · Article · Feb 2012 · Journal of Cardiac Surgery
[Show abstract][Hide abstract] ABSTRACT: Proline is one of the most important osmoregulatory solutes subjected to osmotic stresses. In this study, low nitrogen supply
suppressed the dry biomass, leaf area, and proline biosynthesis of the seedlings of the energy plant Jatropha curcas, which could grow in poor, dry soil. Low-nitrogen stress induced JcP5CS mRNA expression and decreased the activity of P5CS enzyme and the content of free proline in leaves of J. curcas seedlings. When the seedlings grown in low-nitrogen conditions were suddenly exposed to PEG-6000 (−1.6MPa) stress, the expression
of JcP5CS gene was highly induced, and both the activity of P5CS and the content of free proline increased and maintained at high levels
to mitigate the impact of drought stresses. This may be one of the reasons why J. curcas could adapt to poor and drought conditions.
KeywordsLow nitrogen supply–Proline–Drought stress–P5CS–
No preview · Article · Sep 2011 · Acta Physiologiae Plantarum
[Show abstract][Hide abstract] ABSTRACT: A D laser scanning system for rock surface measurement was developed based on structured light measuring technique. The structure of the system was designed. A fast calibration method for the D laser scanning system was presented in detail. After the system was calibrated, the measuring precision was verified in the depth direction. Experimental results proved that the measuring precision of the system is better than 0.02mm. And the system can achieve high performance during practical application.
No preview · Article · Jun 2011 · Proceedings of SPIE - The International Society for Optical Engineering
[Show abstract][Hide abstract] ABSTRACT: N-heterocyclic carbene ligands( NHCs) are playing an increasingly important role in many areas such as organometallic, organic synthetic, pharmaceutical and polymer chemistry. While numerous powerful catalytic systems incorporating NHC ligands have been described, methods of synthesizing them have advanced more slowly. This review describes the recent progress of the methods used in the preparation of N-heterocyclic carbene complexes. According to the nature of the NHC precursor and to the activation method employed, the common routes for preparing NHC-metal complexes are insertion of metal ions into the carbon-carbon double bonds of highly electron-rich alkenes, coordination of preformed, isolated free carbenes, deprotonation of an Imidazolium salt with an external base or with metal complex having a basic ligand, thermal decomposition of corresponding carbene adducts, transmetallation from a silver-NHC complex or from a gold-NHC complex, oxidative addition of the C2-X (X= Me, halogen, H) bond of an imidazolium precursor. In addition, our research group firstly found that iron, cobalt, nickel, and copper complexes of N-heterocyclic carbenes can be directly synthesized by using commercially available metal powders.
No preview · Article · Mar 2011 · Chemical Communications
[Show abstract][Hide abstract] ABSTRACT: 2-(4-Bromophenyl)-3-(4-diphenylaminophenyl)acrylonitrile was prepared by the base catalysed condensation of 4-diphenylaminobenzaldehyde and 2-(4-bromophenyl)acetonitrile. A series of α,β-diarylacrylonitrile derivatives containing both a biphenyl group and a triphenylamine unit were synthesized from 2-(4-bromophenyl)-3-(4-diphenylaminophenyl)acrylonitrile and boronic acids via a Suzuki reaction. The structures of these compounds were characterized using FTIR, 1H NMR, 13C NMR, MS and elemental analysis. The UV–vis absorption and photoluminescent spectra of these derivatives were investigated. These compounds emit green fluorescence with maximum emission peaks of 500–550 nm in CH2Cl2 solution and in the solid state under UV irradiation. Analysis revealed good thermal stability with a decomposition temperature ranging from 279–386 °C.
No preview · Article · Mar 2011 · Dyes and Pigments