Michele M. Schantz

National Institute of Standards and Technology, GAI, Maryland, United States

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Publications (139)320.55 Total impact

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    ABSTRACT: During native subsistence hunts from 1987 to 2007, blubber and liver samples from 50 subadult male northern fur seals (Callorhinus ursinus) were collected on St. Paul Island, Alaska. Samples were analyzed for legacy persistent organic pollutants (POPs), recently phased-out/current-use POPs, and vitamins. The legacy POPs measured from blubber samples included polychlorinated biphenyl congeners, DDT (and its metabolites), chlorobenzenes, chlordanes, and mirex. Recently phased-out/current-use POPs included in the blubber analysis were the flame retardants, polybrominated diphenyl ethers, and hexabromocyclododecanes. The chemical surfactants, perfluorinated alkyl acids, and vitamins A and E were assessed in the liver samples. Overall, concentrations of legacy POPs are similar to levels seen in seal samples from other areas of the North Pacific Ocean and the Bering Sea. Statistically significant correlations were seen between compounds with similar functions (pesticides, flame retardants, vitamins). With sample collection spanning two decades, the temporal trends in the concentrations of POPs and vitamins were assessed. For these animals, the concentrations of the legacy POPs tend to decrease or stay the same with sampling year; however, the concentrations of the current-use POPs increased with sampling year. Vitamin concentrations tended to stay the same across the sampling years. With the population of northern fur seals from St. Paul Island on the decline, a detailed assessment of exposure to contaminants and the correlations with vitamins fills a critical gap for identifying potential population risk factors that might be associated with health effects.
    Full-text · Article · Jul 2015 · Archives of Environmental Contamination and Toxicology
  • Stephen A. Wise · Lane C. Sander · Michele M. Schantz
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    ABSTRACT: The identification of 16 polycyclic aromatic hydrocarbons (PAHs) as priority pollutants by the U.S. Environmental Protection Agency (EPA) in 1976 has been a primary driver for analytical methods development for the determination of PAHs. In this article, the historical development of methods in liquid chromatography (LC) and gas chromatography (GC) to separate these 16 PAHs is discussed. In LC a significant effort was the search for and the fundamental understanding of the unique stationary phase capable of achieving the desired separation of the 16 EPA PAHs. For GC methods, the focus on stationary phase development has been the separation of critical isomers with a broader scope than the 16 EPA PAHs. The current routine LC and GC methods for the 16 EPA PAHs are well established; however, new advances in analytical techniques beyond LC and GC are discussed. Many analysts are now interested in more than just the 16 EPA PAHs (e.g., higher molecular mass PAHs and alkyl-substituted PAHs) and analytical methods have emerged to address these needs. Reference materials and their use in the determination of PAHs are discussed.
    No preview · Article · Mar 2015 · Polycyclic Aromatic Compounds
  • Michele M Schantz · Rebecca S Pugh · Stacy S Vander Pol · Stephen A Wise
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    ABSTRACT: The stability of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and chlorinated pesticides in frozen mussel tissue Standard Reference Materials (SRMs) stored at -80 °C was assessed by analyzing samples of SRM 1974, SRM 1974a, and SRM 1974b Organics in Mussel Tissue (Mytilus edulis) periodically over 25 y, 20 y, and 12 y, respectively. The most recent analyses were performed during the certification of the fourth release of this material, SRM 1974c. Results indicate the concentrations of these persistent organic pollutants have not changed during storage at -80 °C. In addition, brominated diphenyl ethers (BDEs) were quantified in each of the materials during this study. The stability information is important for on-going monitoring studies collecting large quantities of samples for future analyses (i.e., formally established specimen banking programs). Since all four mussel tissue SRMs were prepared from mussels collected at the same site in Dorchester Bay, MA, USA, the results provide a temporal trend study for these contaminants over a 17 year period (1987 to 2004).
    No preview · Article · Feb 2015 · Analytical and Bioanalytical Chemistry
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    ABSTRACT: Two new Standard Reference Materials (SRMs), SRM 3672 Organic Contaminants in Smokers’ Urine (Frozen) and SRM 3673 Organic Contaminants in Non-Smokers’ Urine (Frozen), have been developed in support of studies for assessment of human exposure to select organic environmental contaminants. Collaborations among three organizations resulted in certified values for 11 hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) and reference values for 11 phthalate metabolites, 8 environmental phenols and parabens, and 24 volatile organic compound (VOC) metabolites. Reference values are also available for creatinine and the free forms of caffeine, theobromine, ibuprofen, nicotine, cotinine, and 3-hydroxycotinine. These are the first urine Certified Reference Materials characterized for metabolites of organic environmental contaminants. Noteworthy, the mass fractions of the environmental organic contaminants in the two SRMs are within the ranges reported in population survey studies such as the National Health and Nutrition Examination Survey (NHANES) and the Canadian Health Measures Survey (CHMS). These SRMs will be useful as quality control samples for ensuring compatibility of results among population survey studies and will fill a void to assess the accuracy of analytical methods used in studies monitoring human exposure to these organic environmental contaminants. Graphical Abstract Metabolites of PAHs, Phthalates, Phenols, Parabens, and VOCs in Urine SRMs
    Full-text · Article · Feb 2015 · Analytical and Bioanalytical Chemistry

  • No preview · Article · Jul 2014 · Metrologia
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    ABSTRACT: The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health (NIH), has developed a Standard Reference Material (SRM) to support technology development in metabolomics research. SRM 1950 Metabolites in Human Plasma is intended to have metabolite concentrations that are representative of those found in adult human plasma. The plasma used in the preparation of SRM 1950 was collected from both male and female donors, and donor ethnicity targets were selected based upon the ethnic makeup of the U.S. population. Metabolomics research is diverse in terms of both instrumentation and scientific goals. This SRM was designed to apply broadly to the field, not towards specific applications. Therefore, concentrations of approximately 100 analytes, including amino acids, fatty acids, trace elements, vitamins, hormones, selenoproteins, clinical markers, and perfluorinated compounds (PFCs), were determined. Value assignment measurements were performed by NIST and the Centers for Disease Control and Prevention (CDC). SRM 1950 is the first reference material developed specifically for metabolomics research.
    No preview · Article · Nov 2013 · Analytical Chemistry
  • Margarete S Nocun · Michele M Schantz
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    ABSTRACT: Oxygenated polycyclic aromatic hydrocarbons (oxy-PAHs) have recently received much attention in discussions regarding the negative impacts of particulate matter (PM) on human health and the environment. The National Institute of Standards and Technology provides several environmental matrix standard reference materials (SRMs) with certified and reference values for polycyclic aromatic hydrocarbons (PAHs) and nitrated PAHs. In this study, the concentrations of oxygenated PAHs are determined in three air PM SRMs (1649b, 1648a, and 2786) and three diesel PM SRMs (1650b, 2975, and 1975) using two independent gas chromatography-mass spectrometry methods. Concentrations of oxy-PAHs were at the milligrams per kilogram level with higher overall concentrations in diesel PM (up to 50 mg/kg for 9,10-anthraquinone). One of the highest oxy-PAH concentrations (up to 5 mg/kg) measured in the air particulate SRMs was for 7,12-benz[a]anthracenquinone. These results suggest that oxygenated PAHs should not be neglected in the analysis of PM as their concentrations can be as high as those of some PAHs and are one to two orders of magnitude higher than those for nitro-PAHs.
    No preview · Article · Apr 2013 · Analytical and Bioanalytical Chemistry
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    ABSTRACT: The National Institute of Standards and Technology administers quality assurance programs devoted to improving measurements of nutrients and related metabolites in foods, dietary supplements, and serum and plasma samples. These programs have been developed in collaboration with the National Institutes of Health to assist measurement communities in their efforts to achieve accurate results that are comparable among different laboratories and over time. Targeted analytes include micronutrients, botanical markers, nutritional elements, contaminants, fatty acids, and vitamin D metabolites.
    Full-text · Article · Apr 2013 · Analytical and Bioanalytical Chemistry
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    ABSTRACT: As part of a collaboration with the National Institutes of Health’s Office of Dietary Supplements and the Food and Drug Administration’s Center for Drug Evaluation and Research, the National Institute of Standards and Technology has developed Standard Reference Material (SRM) 3274 Botanical Oils Containing Omega-3 and Omega-6 Fatty Acids and SRM 3275 Omega-3 and Omega-6 Fatty Acids in Fish Oil. SRM 3274 consists of one ampoule of each of four seed oils (3274-1 Borage (Borago officinalis), 3274-2 Evening Primrose (Oenothera biennis), 3274-3 Flax (Linium usitatissimum), and 3274-4 Perilla (Perilla frutescens)), and SRM 3275 consists of two ampoules of each of three fish oils (3275-1 a concentrate high in docosahexaenoic acid, 3275-2 an anchovy oil high in docosahexaenoic acid and eicosapentaenoic acid, and 3275-3 a concentrate containing 60 % long-chain omega-3 fatty acids). Each oil has certified and reference mass fraction values for up to 20 fatty acids. The fatty acid mass fraction values are based on results from analyses using gas chromatography with flame ionization detection (GC-FID) and mass spectrometry (GC/MS). These SRMs will complement other reference materials currently available with mass fractions for similar analytes and are part of a series of SRMs being developed for dietary supplements. Figure Components of SRM 3274 Botanical Oils Containing Omega-3 and Omega-6 Fatty Acids
    Full-text · Article · Feb 2013 · Analytical and Bioanalytical Chemistry

  • No preview · Article · Jan 2013 · Metrologia
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    ABSTRACT: Ethanol is important both forensically ("drunk driving" or driving while under the influence, "DWI", regulations) and commercially (alcoholic beverages). Blood-and breath-alcohol testing can be imposed on individuals operating private vehicles such as cars, boats, or snowmobiles, or operators of commercial vehicles like trucks, planes, and ships. The various levels of blood alcohol that determine whether these operators are considered legally impaired vary depending on the circumstances, and locality Accurate calibration and validation of instrumentation is critical in areas of forensic testing where quantitative analysis directly affects the outcome of criminal prosecutions, as is the case with the determination of ethanol in blood and breath. Additionally, the accurate assessment of the alcoholic content of beverages is a commercially important commodity. In 2002, the CCQM conducted a Key Comparison (CCQM-K27) for the determination of ethanol in aqueous matrix with nine participants. A report on this project has been approved by the CCQM and can be found at the BIPM website [1]. CCQM-K27 was comprised of three samples, one at low mass fraction of ethanol in water (nominal concentration of 0.8 mg/g), one at high level (nominal concentration of 120 mg/g), and one wine matrix (nominal concentration of 81 mg/g). Overall agreement among eight participants using gas chromatography with flame ionization detection (GC-FID), titrimetry, isotope dilution gas chromatography/mass spectrometry (GC-IDMS), and gas chromatography-combustion-isotope ratio mass spectrometry (ID-GC-C-IRMS) was good. The ninth participant used a headspace GC-FID method that had not been validated in an earlier pilot study (CCQM-P35). A follow-on Key Comparison, CCQM-K27-Subsequent, was initiated in 2003 to accommodate laboratories that had not been ready to benchmark their methods in the original CCQM-K27 study or that wished to benchmark a different method. Four levels of ethanol in water were used in the subsequent study (nominal concentrations of 0.2 mg/g, 1 mg/g, 3 mg/g, and 60 mg/g). The three participants in the CCQM-K27-Subsequent Key Comparison demonstrated their ability to measure ethanol in aqueous matrix in the concentration range of 0.2 mg/g to 60 mg/g. A report on this project has been approved by the CCQM and can be found at the BIPM website [1]. A second follow-on Key Comparison, CCQM-K27.2 Second Subsequent, was initiated in 2006 to accommodate laboratories that had not been ready to benchmark their methods in the previous two CCQM-K27 studies. Two levels of ethanol in water were used in the second subsequent study ranging in concentration between 0.5 mg/g and 4 mg/g. Four of the five participants in the CCQM-K27.2 Second Subsequent Key Comparison demonstrated their ability to measure ethanol in aqueous matrix in that concentration range.
    No preview · Article · Jan 2013 · Metrologia
  • L.C. Sander · M.M. Schantz · S.A. Wise
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    ABSTRACT: The term persistent organic pollutants (POPs) describes a broad range of anthropogenic compounds present in the environment. Persistent organic pollutants can be grouped into subclasses based on their original intended uses or chemical properties. In this chapter, five such groups of compounds are considered: polycyclic aromatic hydrocarbons (PAHs), chlorinated aromatic compounds, pesticides, brominated flame retardants (BFRs), and perfluoroalkyl and polyfluoroalkyl substances (PFASs). The chapter provides an overview of some of the measurement issues and liquid chromatography (LC) methods used in the determination of persistent organic contaminants in environmentally relevant samples. Emphasis has been placed on research carried out since 2000. Purely gas chromatography (GC)-based methods have been excluded from this discussion, as have reports that emphasize sample cleanup and processing research.
    No preview · Article · Jan 2013

  • No preview · Article · Jan 2013 · Metrologia
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    ABSTRACT: Four new Standard Reference Materials (SRMs) have been developed to assist in the quality assurance of chemical contaminant measurements required for human biomonitoring studies, SRM 1953 Organic Contaminants in Non-Fortified Human Milk, SRM 1954 Organic Contaminants in Fortified Human Milk, SRM 1957 Organic Contaminants in Non-Fortified Human Serum, and SRM 1958 Organic Contaminants in Fortified Human Serum. These materials were developed as part of a collaboration between the National Institute of Standards and Technology (NIST) and the Centers for Disease Control and Prevention (CDC) with both agencies contributing data used in the certification of mass fraction values for a wide range of organic contaminants including polychlorinated biphenyl (PCB) congeners, chlorinated pesticides, polybrominated diphenyl ether (PBDE) congeners, and polychlorinated dibenzo-p-dioxin (PCDD) and dibenzofuran (PCDF) congeners. The certified mass fractions of the organic contaminants in unfortified samples, SRM 1953 and SRM 1957, ranged from 12 ng/kg to 2200 ng/kg with the exception of 4,4'-DDE in SRM 1953 at 7400 ng/kg with expanded uncertainties generally <14 %. This agreement suggests that there were no significant biases existing among the multiple methods used for analysis.
    Full-text · Article · Nov 2012 · Analytical and Bioanalytical Chemistry
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    ABSTRACT: Drinking yerba maté, common in southern South America, may increase the risk of esophageal squamous cell carcinoma (ESCC). In 2006, we found high but variable polycyclic aromatic hydrocarbon (PAH) content in commercial yerba maté samples from eight Brazilian brands. The PAH content of new samples from the same brands, purchased in 2008, and four brands from a single manufacturer processed in different ways, obtained in 2010, were quantified to determine whether PAH concentration was still high, PAH content variation was brand specific, and whether processing method affects PAH content of commercial yerba maté. Concentrations of individual PAHs were quantified using gas chromatography/mass spectrometry with deuterated PAHs as internal standards. Median total PAH concentration was 1500 ng/g (range: 625 to 3710 ng/g) and 1090 ng/g (621 to 1990 ng/g) in 2008 and 2010 samples, respectively. Comparing 2006 and 2008 samples, some brands had high PAH concentrations in both years, while PAH concentration changed considerably in others. Benzo[a]pyrene concentrations ranged from 11.9 to 99.3 ng/g and 5.11 to 21.0 ng/g in 2008 and 2010 samples, respectively. The 2010 sample processed without touching smoke had the lowest benzo[a]pyrene content. These results support previous findings of very high total and carcinogenic PAH concentrations in yerba maté, perhaps contributing to the high incidence of ESCC in southern South America. The large PAH content variation by brand, batch and processing method suggests it may be possible to reduce the content of carcinogenic PAHs in commercial yerba maté, making it a healthier beverage.
    Full-text · Article · Oct 2012 · Environmental Science & Technology
  • Michele M Schantz · Elizabeth McGaw · Stephen A Wise
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    ABSTRACT: Four particulate matter Standard Reference Materials (SRMs) available from the National Institute of Standards and Technology (NIST) were used to evaluate the effect of solvent, number of static cycles and static times, pressure, and temperature when using pressurized liquid extraction (PLE) for the extraction of polycyclic aromatic hydrocarbons (PAHs) and nitrated-PAHs. The four materials used in the study were SRM 1648a Urban Particulate Matter, SRM 1649b Urban Dust, SRM 1650b Diesel Particulate Matter, and SRM 2975 Diesel Particulate Matter (Industrial Forklift). The results from the study indicate that the choice of solvent, dichloromethane compared to toluene and toluene/methanol mixtures, had little effect on the extraction efficiency. With three to five extraction cycles, increasing the extraction time for each cycle from 5 to 30 min had no significant effect on the extraction efficiency. The differences in extraction efficiency were not significant (with over 95% of the differences being <10%) when the pressure was increased from 13.8 to 20.7 MPa. The largest increase in extraction efficiency occurred for selected PAHs when the temperature of extraction was increased from 100 to 200 °C. At 200 °C naphthalene, biphenyl, fluorene, dibenzothiophene, and anthracene show substantially higher mass fractions (>30%) than when extracted at 100 °C in all the SRMs studied. For SRM 2975, large increases (>100%) are also observed for some other PAHs including benz[a]anthracene, benzo[k]fluoranthene, benzo[e]pyrene, benzo[a]pyrene, benzo[ghi]perylene, and benzo[b]chrysene when extracted at the higher temperatures; however, similar trends were not observed for the other diesel particulate sample, SRM 1650b. The results are discussed in relation to the use of the SRMs for evaluating analytical methods.
    No preview · Article · Sep 2012 · Analytical Chemistry
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    ABSTRACT: Linzhou, China has one of the highest rates of esophageal squamous cell carcinoma in the world. Exposure to carcinogenic polycyclic aromatic hydrocarbons (PAHs), such as benzo[a]pyrene (BaP), may have a role in this increased risk. To better understand PAH sources, we measured PAHs in the air and food of 20 non-smokers over multiple days and compared the concentrations with a urinary PAH biomarker, 1-hydroxypyrene glucuronide (1-OHPG). Sampling occurred over 4 consecutive days. Kitchen air samples (days 2-3) and duplicate diet samples (days 1-4) were analyzed for 14 or more unique PAHs, including BaP. Daily urine samples (days 1-3) were analyzed for 1-OHPG. Mixed-effects models were used to evaluate the associations between air or food PAH concentrations and urine 1-OHPG concentrations. The median kitchen air BaP concentration was 10.2 ng/m(3) (interquartile range (IQR): 5.1-20.2 ng/m(3)). The median daily food BaP concentration and intake were 0.08 ng/g (IQR=0.04-0.16 ng/g) and 86 ng/day (IQR=41-142 ng/day), respectively. The median 1-OHPG concentration was 3.36 pmol/ml (IQR=2.09-6.98 pmol/ml). In mixed-effects models, 1-OHPG concentration increased with same-day concentration of food BaP (P=0.07). Although PAH concentrations in air were not associated with 1-OHPG concentrations, the high concentrations of PAHs in both air and food suggest that they are both important routes of exposure to PAHs in this population. Further evaluation of the role of PAH exposure from air and food in the elevated rates of esophageal cancer in this region is warranted.Journal of Exposure Science and Environmental Epidemiology advance online publication, 18 July 2012; doi:10.1038/jes.2012.73.
    No preview · Article · Jul 2012 · Journal of Exposure Science and Environmental Epidemiology

  • No preview · Article · Jul 2012 · Reproductive Toxicology
  • Stephen A Wise · Karen W Phinney · Lane C Sander · Michele M Schantz
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    ABSTRACT: The certification of chemical constituents in natural-matrix Standard Reference Materials (SRMs) at the National Institute of Standards and Technology (NIST) can require the use of two or more independent analytical methods. The independence among the methods is generally achieved by taking advantage of differences in extraction, separation, and detection selectivity. This review describes the development of the independent analytical methods approach at NIST, and its implementation in the measurement of organic constituents such as contaminants in environmental materials, nutrients and marker compounds in food and dietary supplement matrices, and health diagnostic and nutritional assessment markers in human serum. The focus of this review is the important and critical role that separation science techniques play in achieving the necessary independence of the analytical steps in the measurement of trace-level organic constituents in natural matrix SRMs.
    No preview · Article · Jun 2012 · Journal of Chromatography A
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    ABSTRACT: Standard reference materials (SRMs) are homogeneous, well-characterized materials used to validate measurements and improve the quality of analytical data. The National Institute of Standards and Technology (NIST) has a wide range of SRMs that have mass fraction values assigned for legacy pollutants. These SRMs can also serve as test materials for method development, method validation, and measurement for contaminants of emerging concern. Because inter-laboratory comparison studies have revealed substantial variability of measurements of perfluoroalkyl acids (PFAAs), future analytical measurements will benefit from determination of consensus values for PFAAs in SRMs to provide a means to demonstrate method-specific performance. To that end, NIST, in collaboration with other groups, has been measuring concentrations of PFAAs in a variety of SRMs. Here we report levels of PFAAs and perfluorooctane sulfonamide (PFOSA) determined in four biological SRMs: fish tissue (SRM 1946 Lake Superior Fish Tissue, SRM 1947 Lake Michigan Fish Tissue), bovine liver (SRM 1577c), and mussel tissue (SRM 2974a). We also report concentrations for three in-house quality-control materials: beluga whale liver, pygmy sperm whale liver, and white-sided dolphin liver. Measurements in SRMs show an array of PFAAs, with perfluorooctane sulfonate (PFOS) being the most frequently detected. Reference and information values are reported for PFAAs measured in these biological SRMs. Figure NIST SRMs 1946 Lake Superior Fish Tissue and 1947 Lake Michigan Fish Tissue
    Full-text · Article · Apr 2012 · Analytical and Bioanalytical Chemistry