[Show abstract][Hide abstract] ABSTRACT: The morbidity and mortality rates of very premature infants are essential for prenatal counseling and quality control.Methods: We reviewed all infants with GA< 32w and BW< 1500gr admitted during the years 2006-2009 to a single neonatal intensive care unit.Results: The survival rate until hospital discharge for infants admitted to NICU and early morbidity are shown in table 1 and 2.
[Show abstract][Hide abstract] ABSTRACT: Septicemia due to Bacillus pumilus, with or without the coexistence of predisposing factors, is extremely rare in the literature. The genus Bacillus includes aerobic and optionally anaerobic Grampositive bacteria that rarely cause clinical infection, mainly in immunocompromised patients. Two cases of septicemia due to B. pumilus in two full-term neonates, one in an 8-day-old female infant without risk factors and the other in a 19-day-old male infant in mechanical ventilation with intravenous catheter, are described. In both cases the microorganism was recovered from repeated blood cultures and was identified using biochemical and molecular assays. Both infants were successfully treated with vancomycin.
[Show abstract][Hide abstract] ABSTRACT: Plasmalogens are phospholipids characterized by the presence of a vinyl ether bond at the sn-1 position of the glycerol backbone. They are particularly abundant in the nervous system, the heart and striated muscle. Peroxisomes are essential for their biosynthesis and red blood cell (RBC) plasmalogen levels are a reliable test in the investigation of patients suspect for a peroxisomal defect. The functions attributed to them include protection against oxidative stress, myelin formation and signal transduction. The aim of the present study was the investigation of RBC plasmalogen levels in neonates.
A total of 25 healthy full-term, appropriate for gestational age neonates were studied. RBC plasmalogens were estimated using gas chromatography within the first five days of life. Fifteen healthy children 1-8-year olds served as controls.
Statistically significant lower plasmalogen levels were found in neonates compared to older children.
Our results indicate that a different range of normal values for plasmalogen levels should be used in the investigation of peroxisomal diseases in neonates. The lower levels of plasmalogens in neonates found in our study could render them more vulnerable to oxidative stress.
No preview · Article · May 2009 · Acta Paediatrica
[Show abstract][Hide abstract] ABSTRACT: Long-chain polyunsaturated fatty acids (LC-PUFA) are important for fetal and infant growth and development. The effects of prematurity and perinatal asphyxia on the levels of linoleic acid (LA) and arachidonic acid (AA) in plasma and red blood cell (RBC) membranes were investigated.
Fifty-five neonates were studied: 18 full term neonates with perinatal asphyxia (group A), nine preterm neonates (group B), and 28 healthy term neonates (group C). Non-esterified and total levels of LA and AA in plasma and RBC membranes were estimated using gas chromatography within the first day of life. Malondialdehyde (MDA) levels were measured using the thiobarbituric acid (TBA) reactivity method.
Compared to group C, statistically significant lower levels of plasma free and total AA and free LA were observed in group A, whereas statistically significant higher levels of RBC total LA and AA were observed in RBC membranes of group B. A negative correlation between MDA and LC-PUFA levels was found.
Perinatal asphyxia is associated with a reduction in LC-PUFA levels, most likely as a result of increased oxidative stress. Premature infants soon after birth have higher LC-PUFA levels than term neonates, probably reflecting the overall metabolic activity and/or intrauterine transport of LC-PUFA.
No preview · Article · Sep 2007 · Journal of Maternal-Fetal and Neonatal Medicine
[Show abstract][Hide abstract] ABSTRACT: OBJECTIVE: To investigate the behavior of chitotriosidase, a human chitinolytic enzyme produced mainly by activated macrophages, in neonates with fungal and common microbial infections. METHOD: Chitotriosidase activity was assayed in serial plasma and urine samples from 8 neonates with fungal infections (7/8 Candida albicans, 1/8 Aspergillus niger). At least 4 samples were investigated per neonate at one week intervals. Chitotriosidase activity was also determined in plasma and urine samples from 15 neonates on diagnosis of Gram-positive and Gram-negative infections. RESULTS Increased chitotriosidase activity was found in plasma and/or urine in 7/8 neonates with fungal infections, ranging from 1.25-9 and 1.9-150 times the upper normal limits in plasma and urine respectively. Improvement of the clinical condition was associated with decline in chitotriosidase activity and vice versa. Increased activity, 1.9-12.5 and 1.5-40 times the upper normal limits in plasma and urine respectively, was also found in 13/15 neonates with bacterial infections. CONCLUSIONS: Increased activity of chitotriosidase is observed not only in fungal but also in microbial infections. As a result chitotriosidase activity cannot be used as a specific marker for fungal infections. However serial assays of the enzyme could be of value in monitoring the outcome of treatment of neonates with fungal infection.
No preview · Article · Mar 2006 · Archives of Hellenic Medicine
[Show abstract][Hide abstract] ABSTRACT: Increased plasma and/or urine chitotriosidase activity was found in neonates with fungal infection changing in parallel with their clinical condition. Increased levels were also found in neonates with bacterial infection. Chitotriosidase activity increase is not a response specific to fungi, but serial assays could monitor the course of neonatal fungal infection.
Full-text · Article · Dec 2005 · Archives of Disease in Childhood - Fetal and Neonatal Edition
[Show abstract][Hide abstract] ABSTRACT: A striking elevation of plasma chitotriosidase activity, greater than 150 times the normal median value, was found in two galactosialidosis patients. Furthermore, increased plasma chitotriosidase activity, 10-53 times the normal median value, was also observed in fucosidosis, glycogen storage disease type IV, Alagille syndrome and hydrops fetalis due to congenital herpes virus infection.
No preview · Article · Feb 2004 · Journal of Inherited Metabolic Disease