Wei-dong Tian

West China Hospital of Stomatology, Hua-yang, Sichuan, China

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Publications (54)10.83 Total impact

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    ABSTRACT: To develop a new threshold segmentation method for mandible image segmentation. CT data of 12 volunteers were exported into Mimics 10. 01. An improved method usinga narrowed threshold range (the maximum threshold range that can segment mandible without manual efforts) was developed in 3D reconstruction, and compared with the traditional method. We used dilation operations to make up the information loss of image borders, by which we obtained an approxinate segment result. A precise segment resultwas eventually arrived with the help of logical operations and region growing. We compared mean time consumptions of the two methods, as well as their 3D reconstruction results using Geomagic Studio 11. 0. The new method generated a success rate of 91. 67% (11/12), with a mean time consumption of (319. 7±125. 3) s. The traditional method took much longer time [(1,261. 3±427. 3) s, P<0. 05] than the new method. Compared with the reconstruction results of traditional method, the new method had an outward deviation of (0. 066±0. 011) mm and an inward deviation of (0. 070±0. 008) mm. Such deviations were less than the minimum distance that a naked eye can discern. The lower limit of the widest threshold range which mandible could be isolated was (507. 72± 100. 31) HU, while the upper limit was (1,133. 33±47. 57) HU. The new method we proposed can improve the efficiency of threshold segmentation of mandible.
    No preview · Article · May 2015 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
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    ABSTRACT: This study investigates the effects of the Wnt signaling pathway on the osteogenic differentiation of human adipose-derived stem cells (hASCs) under tensile stress. hASCs cultured in vitro were divided into 4 groups: Group A, hASCs; Group B, Wnt5a RNAi-treated hASCs; Group C, hASCs under tensile stress; and Group D, Wnt5a RNAi-treated hASCs under tensile stress. Five days after treatment, the genes associated with the Wnt/β-catenin and Wnt/Ca2+ pathways were analyzed in all groups by real-time RT-PCR; the Wnt10b, Wnt5a, RUNX2 and SPP1 proteins were analyzed by western blot analysis. Compared with the expression in Groups A and B, all the genes and proteins in Groups C and D had higher expression, except for Wnt5ain Group D. Compared with Group C, Wnt5a, RhoA ,RUNX2 and ALPL had lower expression in Group D, but the markers associated with Wnt/β-catenin had higher expression. The results suggest that tensile stress can promote maturation and osteogenic differentiation in hASCs and also activate the Wnt/β-catenin and Wnt/Ca2+ pathways. The Wnt/Ca2+ pathway may have the potential to inhibit the Wnt/β-catenin pathway. Wnt5a knock down seemed to increase the expression of the Wnt/β-catenin pathway, which is activated by Wnt10b.
    Preview · Article · Apr 2015 · Journal of Hard Tissue Biology
  • Shuang Liu · Hai-sen Huang · Mei Yu · Xiao-rin Luo · Wei-hua Guo · Wei-dong Tian
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    ABSTRACT: To determine the effects of exogenous nitric oxide (NO) donor sodium nitroprusside (SNP) on the proliferation and expression of related-gene of adipose-derived stem cells(ADSCs) and its role in chondrogenic differentiation of ADSCs. Rat ADSCs were harvested and cultured, and then induced to osteogenic and adipogenic differentiations, detected with Alizarin red stained and Oil red O stain, respectively. The change of NO during chondrogenic differentiation of ADSCs was tested by NO detection kit. Cell counting kit-8 (CCK8) was used to detect the proliferation of ADSCs under different concentrations of SNP (0.25 mmol/L, 1.00 mmol/L, 4.00 mmol/L). Gene expression level of transformation growth factor (TGF-beta1) and specific gene of chondrogenic differentiation-signaling protein Smad3 and Collage II alPHA (Col-II alpha1), were detected by Real time- PCR (RT-PCR) method. Positive alizarin red staining and Oil red O staining were found after osteogenic and adipogenic induction of cultured ADSCs. Higher concentrations of NO were found in the supernatant of the experimental group with ADSCs chondrogenic differentiations compared with the controls (P<0.05). Low concentrations (0. 25 mmol/L, 1.00 mmol/L) of SNP showed no significant effects on cell proliferations (P>0.05), whereas high concentration (4. 00 mmol/L) of SNP inhibited cell proliferation (P<0.05). RT-PCR revealed that SNP inhibited the gene expression of TGF-beta1 mRNA and chondrogenic differentiation of specific gene Smad3 mRNA, Col-II alpha1 mRNA. SNP can inhibit chondrogenic differentiations by suppressing the production of TGF-beta1 and inhibiting downstream of TGF-beta1 signaling pathways, thereby inhibiting ADSCs differentiation into chondrocytes.
    No preview · Article · Mar 2015 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
  • Hai-Sen Huang · Shuang Liu · Mei Yu · Xiao-Xin Luo · Wei-Hua Guo · Wei-Dong Tian
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    ABSTRACT: Unlabelled: OBEJECTIVE: To construct miRNA-expressing plasmid vector for interfering the expression of beta-catenin in adipose-derived stem cells from Sprague-Dawley rats. Methods: The double strands oligonucleotides of miR-expressing cassette were ligated into plasmid pSWH to generate pSWH-miR. Then the PCR product of enhanced green fluorescence protein (EGFP) open reading frame (ORF) fragment was inserted into BamH I and Xbo I digested pSWH-miR to generate pSWH-EGFP-miR. The adipose-derived stemcells from rats (rADSCs) were transfected with pSWH-EGFP-miR respectively. The expression of beta-catenin was determined by Western blot at 72 h post-transfection. Results: Five miRNA-expressing plasmid vectors were constructed (miR-780, miR-796, miR-1467, miR-1948, miR-1960). miR-780 and miR-796 had the best silencing effect on the expression of beta-catenin (P < 0.05), less did the miR-1960 (P < 0.05), but not the miR-1467 and the miR-1948 (P < 0.05). Conclusion: miR-780 and miR-796 could interference the beta-catenin in ADSCs with high efficiency.
    No preview · Article · Sep 2014 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
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    ABSTRACT: To investigate the effects of human treated dentin matrix (hTDM) extracellular matrix molecules on odontogenetic and neural differentiation of human dental pulp cells (hDPCs) with an aim to find an effective method to collect extracellular matrix molecules to contribute to reparation dental-pulp complex with dentin defects. hDPCs were obtained and biological characteristics such as source of cells and multi-differentiation potentials were assessed using immunofluorescence and flow cytometry. Fabrication of hTDM extracts and hDPCs was induced with it for 1 week. The odontogenetic differentiation associated genes were tested by qRT-PCR. Results qRT-PCR results showed that cells were higher expression of odontogenetic differentiation associated genes ALP, OPN, OCN, BSP, DMP-1, DSP, beta-III tubulin. The method of extracting extracellular matrix molecules from dentin matrix was effective. The extract liquid provides a suitable microenvironment for odontogenetic and neural differentiation of hDPCs and contributes to reparation dental-pulp complex.
    No preview · Article · Jul 2013 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
  • Ting Li · Yong Li · Wei-Dong Tian · Wei-Hua Guo · Mei Yu · Xiao-Dong Li
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    ABSTRACT: To observe whether fetal bovine serum (FBS) will affect the adipogenic ability of adipose derived stem cells (ADSCs) induced by adipose tissue extract (ATE), and to explore the effects of different FBS protein concentrations in ATE on adipogenic ability, cell proliferation and migration of ADSCs. Rat ADSCs were cultured, passaged, and then subjected to osteogenic and neural induction. The adipose explants were cultured in culture medium with or without FBS, then ATE was collected to induce passage four (P4)-ADSCs, which were subsequently detected with oil red staining on the 7th day and the adipogenic ratio was calculated. Different concentrations of ATE without FBS (100 microg/mL, 250 microg/mL and 500 microg/mL) were used to induce P4-ADSCs before the adipogenic events, and the adipogenic ratio of each concentration group was observed. The effect of different protein concentrations on ADSCs proliferation and migration was also observed. Alizarin red staining and NF immunofluorescence staining were positive after the osteogenic and neural induction of cultured ADSCs. Either ATE with or without FBS was able to induce adipogenic differentiation of ADSCs on the 3rd day and there was no significant differences of adipogenic differentiation between ATE with FBS and without FBS on the 7th day. The adipogenic ratio of 500 microg/mL group was higher than that of 100 pg/mL group and 250 microg/mL group (P < 0.05). After two days of induction, all the three different protein concentrations could inhibit cell proliferation, and different protein concentrations in ATE had no effect on the migration of ADSCs. ATE obtained from culture medium without FBS has no effect on adipogenic capacity of ADSCs in a short period of time. The adipogenic ratio of ADSCs is associated with protein concentration in ATE.
    No preview · Article · Jul 2013 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
  • Kun Li · Feng Li · Li-Juan Guo · Jie Li · Ke Huang · Wei-Hua Guo · Wei-Dong Tian
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    ABSTRACT: To investigate the impact of combined adipose-derived stem cells (ASCs) and platelet-rich plasma (PRP) on the survival of transplanted fat. The ASCs were isolated and cultured from fat tissues by enzyme digestion; and the PRP was prepared by two-step ultracentrifugation. The grafts of fat granules were divided into test groups (ASCs + PRP + fat granules, PRP + fat granules, ASCs + fat granules) and control groups (PBS + fat granules). The grafts were injected into the left and right dorsal subcutaneous areas of nude mice. General observations, volume measurements and microscope examinations were conducted 10 d, 30 d, 60 d and 90 d after transplantation. The grafts of the mice in the ASCs + PRP group showed soft structure, with light yellow color and closer to normal adipose tissue compared with those in other groups. Greater survival volumes (P < 0.05) and better histology were also observed in the grafts of the mice in the ASCs + PRP group. The fat grafts consisting of PRP and ASCs constitute an ideal transplant strategy, which could provide a valuable and needed tool in plastic and reconstructive surgery.
    No preview · Article · May 2013 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
  • Ju-Rong Liao · Jie Li · Ke Huang · Feng Li · Kun Li · Wei-Hua Guo · Mei Yu · Cong Guo · Wei-Dong Tian
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    ABSTRACT: Objective: Study the role for adipose tissue-derived stem cells (ADSCs) of secretome by adipose tissue and hoped that makes some contributions for the fat regeneration. Methods: Direct adherent culture methods and the flow cytometry (FCM) were used to isolate and identify ADSCs. Collected the medium that had contained secreted proteins as the conditional medium (CM) and was used to induce ADSCs, chemical medium group as the positive control and normal medium group as the negative control. The formation of lipid drops was observed through the cell morphology, and the reliability was verified through Real-time PCR of the mRNA level in vitro. To test the effect of CM on ADSCs in vivo, the absorbable gelatin sponge was used as a scaffold for ADSCs. Results: FCM ensured that the cultured cells were ADSCs. Significant increased lipid drops and related mRNA levels were observed in induced ADSCs cultured for 4 days, which indicated that the CM could promote the ADSCs differentiate into adipocytes after cultured for 4 days in vitro. The secreted proteins could promote ADSCS differentiate into vascularization tissues in vivo. Conclusion: The secreted proteome may contain some growth factors that can promote ADSCs to differentiate into adipocytes and vascularization, which may give new directions for the growth factors that could be applied in the repair of soft tissue.
    No preview · Article · Sep 2012 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
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    ABSTRACT: The purpose of this study was to investigate the osteogenic response of human adipose-derived stem cells (hASCs) under mechanical and/or chemical stimulation. hASCs were divided into three groups. In group A, the cells were cultured without any stimulation, in group B, the cells were induced with chemical stimulation, and in group C, the cells were induced with a combination of chemical stimulation and stretch loading. Stretch loading and chemical stimulation were applied using a four-point bending apparatus (0.5 Hz, 2,000 µε, 2 h/day) and osteogenic differentiation medium, respectively. At the 1st, 2nd, 3rd, 5th and 7th day following initiation of stretch loading, we detected alkaline phosphatase activity, mRNA expression (RUNX2, ALPL, osteonectin, osteopontin and type I collagen) and protein expression (RUNX2 and osteopontin) by colorimetric assay, real-time PCR and Western blot methods, respectively. Alkaline phosphatase activity, mRNA expression and protein expression all increased in groups B and C along with the culture time, but were observed to be downregulated by the 7th day in group C (p < 0.05). Compared to group A, most of the above markers were significantly higher in groups B and C (p < 0.05). All of the above markers in group C were higher than those in group B before the 5th day (p < 0.05), except at the 1st day. These results indicated that stretch loading promoted osteogenic differentiation of hASCs and that the combination of mechanical and chemical stimulation could enhance the osteogenic capability up to the 5th day relative to chemical stimulation alone.
    No preview · Article · May 2012 · Cells Tissues Organs
  • Qin-ce Sun · Jin-gang Xiao · Xiao-juan Yang · Wei-dong Tian
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    ABSTRACT: To investigate PPARgamma2 expression and phosphorylation involved in adipogenic differentiations of Adipose-derived Stem Cells (ASCs) with an aim to reveal the possible mechanisms of adipose development. ASCs were obtained from the inguinal fat pads of GFP (green fluorescent protein) mice. The primary cells were cultured in histiocytic attachment cultivation. Adipogenic differentiation of the third generation of ASCs was induced with the conditional medium (alpha-MEM medium supplemented with 100 mL/L fetal bovine serum, 10(-6) mol/L dexamethasone, 10(-5) mol/L insulin, 0. 2 mmol/L indomethacin, and 0.5 mmol/L 3-isobutyl-1-methylxanthine). The levels of PPARgamma2 mRNA and the PPARgamma2 expression and phosphorylation at day 0, 1, 3, 5, 7, and 9 of induction were detected using RT-PCR, fluorescence-immunocytochemistry and western blot, respectively. The expression of PPARgamma2 and phosphorylation were significantly up-regulated during adipogenic differentiation of ASCs. PPARgamma2 is the key regulator of adipose development. The regulation of PPARgamma2 phosphorylation may promote adipogenic differentiation of ASCs.
    No preview · Article · Jan 2011 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
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    ABSTRACT: We investigated the effect of recombinant human bone morphogenetic protein 2 (rhBMP-2) on new bone formation during rapid-rate mandibular distraction osteogenesis. We also explored the feasibility of using local BMP-2 gene therapy to compensate for bad callus formation caused by a rapid distraction rate. Bone marrow mesenchymal stem cells (MSCs) from Japanese rabbits were transfected with adenovirus (adv)-BMP-2. The right mandibles of the rabbits were distracted after corticotomy. The distraction rate in group A was 0.8 mm/d. The distraction rate in group B was 2.4 mm/d, and the distraction gap was injected with adv-lacZ-transfected bone marrow MSCs. The distraction rate in group C was 2.4 mm/d, and the distraction gap was injected with adv-BMP-2-transfected bone marrow MSCs. New generation bone tissue in the distraction gap was analyzed by plain radiograph examinations, microfocus computerized tomography (micro-CT) examinations, and biomechanical tests at weeks 2, 4, and 8 of the consolidation period. Radiographic and micro-CT examinations showed a better bone quality in group C compared with group A at weeks 2 and 4 of the consolidation period. There was no obvious new bone formation in group B. The trabecular parameters (trabecular thickness, trabecular number, volumetric bone mineral density at tissue, and bone volume fraction) were significantly higher in group C than in group A at weeks 2 and 4. At week 8, no significant difference were detected for all parameters except trabecular number between groups A and C. All biomechanical stress parameters were significantly higher in group C than in group A at week 4, and only peak stress was significantly different at week 8. Gene therapy using rhBMP-2-modified MSCs promoted new bone formation during mandibular distraction osteogenesis, and effectively compensated for the detrimental effect of rapid distraction rate on new bone formation.
    No preview · Article · Dec 2010 · Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
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    ABSTRACT: To construct mouse enhanced green fluorecence protein (EGFP) -peroxisome proliferator-activated receptor (PPAR)gamma2, and to detect EGFP-PPARgamma2 expression in infected mouse bone marrow mesenchymal stem cells (BMSC). Cut the fragment of PPARgamma2 from the expression plasmid pcDNA flag PPARgamma2, then cloned the gene fragment into pEGFP-C1 and pEGFP-N1 vector. Subsequently, subclone the fragment EGFP-PPARgamma2 from pEGFP-C1-PPARgamma2 into the shuttle plasmid DC315. HEK293 cells were co-transfected with the constructed recombinant shuttle plasmid DC315-EGFP-PPARgamma2 and large adenovirus helper plasmid pBHGlox deltaE1, 3Cre in mediation of liposome. The obtained replication-defective recombinant adenovirus Ad-EGFP-PPARgamma2 was confirmed. Then it was propagated in HEK293 cells. After the BMSC were transfected for 72 h, adipogenic differentiation was demonstrated. HEK293 cells were transfected with the pEGFP-C1-PPARgamma2 or pEGFP-N1-PPARgamma2 in mediation of liposome. The former green fluorescence protein was better than the latter by fluorescence microscope. The recombinant plasmids were digested and identified. Western blot analysis showed the expression of EGFP-PPARgamma2 in vitro. EGFP-PPARgamma2 protein was detectable in the nucleus of BMSC. The recombinant adenovirus encoding EGFP-PPARgamma2 fusion protein was successfully constructed, which provided a basis for application of EGFP-PPARgamma2 gene to adenovirus-mediated gene therapy.
    No preview · Article · Aug 2010 · Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology
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    Jie Long · Wei Tang · Yu-bo Fan · Wei-dong Tian · Fan Feng · Lei Liu · Xiao-hui Zheng · Wei Jing · Ling Wu
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    ABSTRACT: Distraction osteogenesis typically requires a long treatment period, which can lead to bone and soft-tissue infection and considerable patient discomfort. Use of a rapid distraction rate in craniofacial distraction osteogenesis to shorten the distraction period is possible owing to the unique characteristics of craniofacial bones, including an abundant blood supply and rapid bone healing compared with long bones. The effects of using a rapid distraction rate in the treatment of craniofacial deformities are currently unclear, however. The objective of this study was to investigate the effects of a rapid distraction rate on new bone formation during mandibular distraction osteogenesis in goats. Sixteen goats were randomly divided into four groups consisting of four goats each. In Groups A, B, and C, the right mandible of each goat was distracted at a rate of 0.8mm/d, 1.6mm/d, and 2.0mm/d, respectively; Group D was the control group and did not undergo distraction. Six weeks after the conclusion of distraction, bone densitometry and three-point bending testing were performed in all groups. The mean bone density value of goats in Group A was significantly higher than those of all the other groups (p<0.05), and the mean bone density value of goats in Group C was significantly lower than those of all the other groups (p<0.05). The mean curve slope, peak stress, bending modulus, and energy to failure values of Groups A, B, and C were all significantly lower than those of the control group (p<0.05). As the distraction rate increased, the curve slope and peak stress values gradually declined (p<0.05). Use of a rapid distraction rate in mandibular distraction osteogenesis may have detrimental effects on the quality of new bone, despite the abundant blood supply of craniofacial bones.
    Preview · Article · Feb 2009 · Injury
  • Yong-dai Shen · Yong-hong He · Lin Yang · Wei Tang · Lei Liu · Wei-dong Tian
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    ABSTRACT: To identify the expression of antagonist beta-TrCP protein in Sonic hedgehog signal transduction pathway and Wnt signal transduction pathway in hair follicle tissues. The heads of day 18 embryo, and one day and six-days-old postnatal mice were acquired and treated with 40 g/L paraformaldehyde fixation for 48 h and paraffin embedding. The expression of beta-TrCP proteins was examined using LsAB (labelled streptavidin-biotin) method. beta-TrCP proteins were expressed in the cytoplasm of the hair stems of hair follicle, hair cuticle, cuticle of root sheath, Huxley's layer of internal root sheath cells, external root sheath and mesenchymal tissues, but not in connective tissue sheath and Henle's layer of internal root sheath. TrCP express in the developmental hair follicle tissues, which implicates that beta-TrCP regulate the developmental hair follicle by mediating the signal transduction pathways.
    No preview · Article · Aug 2008 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
  • Hong-Bing Jiang · Wei-Dong Tian · Lai-Kui Liu · Yan Xu
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    ABSTRACT: During tooth development, cranial neural crest (CNC) cells represent a population of pluripotent stem cells that give rise to various dental tissues. This study aimed to investigate whether CNC cells could differentiate into odontoblast-like cells by in vitro induction. CNC cells were isolated from explants of cranial neural tubes and cultured in serum-free Dulbecco's modified Eagle's medium (DMEM)/F12 medium which contained fibroblast growth factor 8 (FGF8) and dentin non-collagen proteins (DNCP). The initiation of controlled differentiation was determined using histological assays, and the expression of specific gene phenotypes was detected using immunocytochemical staining and reverse transcription--polymerase chain reaction (RT--PCR). The first branchial arch phenotype of the CNC cells demonstrated negative Hoxa2 expression and positive vimentin expression in the presence of 100 ng/ml FGF8. Following DNCP induction, the CNC cells became bipolar, demonstrated high alkaline phosphatase (ALP) activity, and formed mineralized nodules. In addition, the expression of DSPP, DMP1, and collagen type I confirmed the odontoblast phenotype. The results indicate that the tissue-specific cellular differentiation (odontoblast-like cells) of early-stage embryonic-derived cells (such as CNC cells) can be induced by adult extracellular matrix proteins (such as DNCP). CNC cells may be used as a valuable cell model for research on dental tissue differentiation and regeneration.
    No preview · Article · Jul 2008 · Cell Biology International
  • Feng Zhu · Rong-Rong Nie · Ling Wu · Lei Liu · Wei Tang · Wei-Dong Tian
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    ABSTRACT: To observe the spontaneous odontogenic differentiation of mouse dental papilla mesenchymal cells in blood serum medium. And to detect the critical gene expression of correlated transcription factors what are specific to odontogenic and osteogenic differentiation. The primary dental papilla mesenchymal cells what had been obtained from E16.5 d murine embryo were serially subcultivated in the simple serum medium and the serum medium supplemented with LIF (leukocyte inhibitory factor) respectively. It was observed whether the dental papilla mesenchymal cells differentiated into odontoblast phenotype or kept the undifferentiation phenotype. The mRNA expression of specific transcription factors were detected in cells with or without odontogenic differentiation. The fourth generation and behind of mouse dental papilla mesenchymal cells what were cultured in simple serum medium could spontaneously differentiate to odontoblast, while the undifferentiation phenotype of dental papilla mesenchymal cells could be lasting to ninth generation when they cultured in medium supplemented with 10(6) U/L LIF. Whether the dental papilla cells differentiate to odontogenic phenotype or not, the members of HOX gene family such as Msx1/Msx2, Pax9 and Lhx6/Lhx7 got completely expression. These transcription factors were specific to odontogenic mesenchymal cells. Also the specific gene of mineralized tissue cells such as DSPP, Sox9, Cbfa1 and Osx initiated to express after the odontoblast differentiation. Not only this spontaneous odontogenic differentiation model of mouse dental papilla mesenchymal cells can be the positive control, but also the mode of gene expression can provide an evidence for studying how gene changes when adult stem cells are induced to odontogenic differentiation.
    No preview · Article · Mar 2008 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
  • Feng Zhu · Rong-Rong Nie · Ling Wu · Lei Liu · Wei Tang · Wei-Dong Tian
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    ABSTRACT: To observe the overexpression of dentin sialophosphoprotein (DSPP) in mouse bone marrow mesenchymal stem cells (BM-MSC) and the expression of specific gene involved in odontogenic and osteogenic differentiation in transfected cells. Mouse bone marrow mesenchymal stem cells (BM-MSC) were cultured and then transfected with DSPP gene by adenovirus-mediated way in vitro. The transfection efficiency of DSPP gene was assessed by the marker gene green fluorescent protein (GFP). With having observed the morphological transformation of transfected BM-MSC, we studied whether the transfected BM-MSC would express odontogenic and osteogenic genes by RT-PCR. The mouse BM-MSC were obtained and the adenovirus mediated DSPP gene used to transfect BM-MSC successfully. The transfected efficiency was 42.7%. The transfected BM-MSC were induced to differentiate into odontoblast-like cells and meanwhile expressed specific odontogenic and osteogenic differentiation genes such as DSPP, DMP1, Msx1/2, Pax9, Lhx6/7, Sox9, Cbfa1, Osx, Col I. The bone marrow mesenchymal stem cells can make differentiation with the overexpression of dentin sialophosphoprotein and express specific odontogenic and osteogenic differentiation genes.
    No preview · Article · Mar 2008 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
  • Xin Nie · Yan Jin · Jie Long · Ling Wu · Wei Jing · Yun-Feng Lin · Wei-Dong Tian
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    ABSTRACT: To construct the tissue culture model in vitro, and investigate the potential of dental pulp fibroblast differentiating into the odontoblast and the promoting role of transforming growth factor beta1 (TGF-beta1) on it. Human pulps were cultured for 3, 7, 14 and 21 days on bone matrix gelatin (BMG) in DMEM cultural medium supplemented with TGF-beta1. The characteristics of matrix were studied through toluidine blue and Mallory stain. Meanwhile, the expression of dentin salivary protein (DSP) on the pulp cells was investigated with immunohistochemical staining. This experiment found that the pulp tissue in vitro were able to develop into more progressive stage, and some pulp fibroblast cells to differentiate into odontoblast-like cells. Toluidine blue and Mallory staining analysis revealed the localized deposition of mineralized bone-dentin matrix that was detected at the site of dental pulp cells. Immunohistochemical analysis proved that the DSP synthesized in these cells with the presence of TGF-beta1. The results demonstrate that this culture condition can maintain the phenotype of human pulp tissue. The model of organ culture is suitable to study the development of pulp tissue in vitro. TGF-beta1 can promote the potential of pulp cell into odontoblast, which provides an academic basis for tooth repair and regeneration.
    No preview · Article · Mar 2008 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
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    ABSTRACT: To construct recombinant adenovirus carrying the mouse dentin caveolin-1 gene using the recombinant adenoviral vector system AdEasy. The cDNA fragment of caveolin-1 was derived from pTRE2-caveolin-1 by restriction enzyme digestion and subcloned into shuttle plasmid pAdtrack-CMV. The resulting plasmid pAdtrack-CMV-caveolin-1, after linearized by digesting with restriction endonuclease Pme I, was transformed into E. coli 1 BJ5183 which had been transformed by adenoviral backbone plasmid pAdEasy-1. The recombinant plasmid pAd-caveolin-1 was screened by kanamycin LB plate and then identified by restriction enzyme digestion. The linearized adenovirus plasmid pAd-caveolin-1 was packaged in 293 cells, then the recombinant adenovirus Ad-caveolin-1 was harvested. The expression of green fluorescence protein was observed under fluorescent microscope. With further amplification and purification, the titer of recombinant adenovirus was determined. The recombinant adenovirus was identified by restriction enzyme digestion analysis and gene sequencing. Cytopathic effect and the expression of GFP were observed in the infected 293 cells. The sequence of caveolin-1 gene insert was the same as that published in the GenBank. The titer of the recombinant adenovirus was 2 x 10(9) pfu/mL. The mouse caveolin-1 recombinant adenovirus was constructed successfully by using AdEasy adenovirus system. Cell transfection and expression of exogenous gene can be detected directly by observing the expression of GFP. These results provide the basis for the further study on the role of caveolin-1 gene in other scopes.
    No preview · Article · Mar 2008 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
  • Xin Nie · Yan Jin · Jie Long · Ling Wu · Wei Jing · Yun-Feng Lin · Wei-Dong Tian
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    ABSTRACT: To compare the growth and development of tissue engineered tooth germ implanted into different tissues, and explore a suitable growing environment for the tissue engineered teeth in vivo. SD rat/porcine tooth germ cells from postnatal 4 days were used as seeding cells, which combined various scaffolding biomaterials to construct the compound with tissue engineered teeth. The allografts were implanted into renal subcapsule, the mesenteries and subcutaneous tissues. Then, the implants were retrieved at special time points for histological analysis. Further developments were not observed in the graft implanted into mesenteries and subcutaneous tissues. Partial grafts were fallen off and lost from the subcutaneous tissues after implanted, and there were obvious lymphocyte infiltrations in the mesenteries. Moreover, the enamel and pulp-dentin complex were observed within the graft implanted in the subrenal capsule, which indicated there to be good condition. The subrenal capsule can provide a promising implantation environment for the further growth of allogeneic tissue engineered tooth germ, and the subrenal capsule implantation can be used as a new alternative method for tissue-engineering tooth in vivo.
    No preview · Article · Mar 2008 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition

Publication Stats

132 Citations
10.83 Total Impact Points

Institutions

  • 2006-2015
    • West China Hospital of Stomatology
      Hua-yang, Sichuan, China
    • Zhejiang University
      • Division of Oral and Maxillofacial Surgery
      Hang-hsien, Zhejiang Sheng, China
  • 2004-2015
    • Sichuan University
      • • State Key Laboratory of Oral Diseases
      • • Department of Oral and Maxillofacial Surgery
      • • Key Laboratory of Oral Biomedical Engineering
      Hua-yang, Sichuan, China
  • 2005
    • Kunming Medical College
      Yün-nan, Yunnan, China